This document summarizes a study on the impact of heavy metal pollution on the molecular genetics of three medicinal plant species. DNA was extracted from plants collected from polluted and non-polluted sites. Inter simple sequence repeats (ISSR) and amplified fragment length polymorphisms (AFLP) markers were used to analyze genetic differences at the molecular level. ISSR analysis generated 172 bands from 5 primers, showing differences between polluted and non-polluted plants. AFLP analysis using 2 primer pairs produced 116 bands, with 79 being polymorphic. Dendograms based on ISSR and AFLP data showed genetic differences between polluted and non-polluted plant populations, indicating heavy metal pollution affects plant genetics at the molecular level
Synthesis of silver nanoparticle using Portulaca oleracea L. extracts. Articl...Nanomedicine Journal (NMJ)
Objective(s):
To evaluate the influences of aqueous extracts of plant parts (stem, leaves, and root) of Portulaca oleracea L. on bioformation of silver nanoparticles (AgNPs).
Materials and Methods:
Synthesis of silver nanoparticles by different plant part extracts of Portulaca oleracea L. was carried out and formation of nanoparticles were confirmed and evaluated using UV-Visible spectroscopy and AFM.
Results:
The plant extracts exposed with silver nitrate showed gradual change in color of the extract from yellow to dark brown. Different silver nanoperticles were formed using extracts of different plant parts.
Conclusion:
It seems that the plant parts differ in their ability to act as a reducing and capping agent.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Studying the expression of proteins in Chrysichthys nigrodigitatus muscle, is essential to understand the biological, physiological and ecological aspects that may be of advantage in ecotoxicology as a tool for biomonitoring the effects of environmental pollution, as well as food safety. This study was aimed to accomplish a systematic characterization of the muscle proteome as well as to identify a putative set of protein biomarkers in C. nigrodigitatus to environmental pollution in Ologe and Badagry lagoons. Fifteen fi sh samples were used
as representative of the population for proteomics analysis. 116 proteins was expressed with 70 up-regulated, 25 down-regulated from Ologe Lagoon and 30 up-regulated, 17 down-regulated proteins from Badagry Lagoon expressed in the muscle of C. nigrodigitatus in response to environmental stressors using iTRAQ, while 8 protein spots from 2-D gels, representing 8 proteins with 2 up-regulated and 6 down-regulated, have been identifi ed using MALDI TOF/TOF MS. Pearson correlation revealed signifi cant correlation (p < 0.05) betwee environmental variables and protein markers. The investigation revealed that the expressed proteins in the muscle of C. nigrodigitatus served as a prognostic tool to assess the fi sh health and pollution status, which was observed that the fi sh were physiologically perturbed by environmental stressors in Ologe and Badagry lagoons.
Synthesis of silver nanoparticle using Portulaca oleracea L. extracts. Articl...Nanomedicine Journal (NMJ)
Objective(s):
To evaluate the influences of aqueous extracts of plant parts (stem, leaves, and root) of Portulaca oleracea L. on bioformation of silver nanoparticles (AgNPs).
Materials and Methods:
Synthesis of silver nanoparticles by different plant part extracts of Portulaca oleracea L. was carried out and formation of nanoparticles were confirmed and evaluated using UV-Visible spectroscopy and AFM.
Results:
The plant extracts exposed with silver nitrate showed gradual change in color of the extract from yellow to dark brown. Different silver nanoperticles were formed using extracts of different plant parts.
Conclusion:
It seems that the plant parts differ in their ability to act as a reducing and capping agent.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Studying the expression of proteins in Chrysichthys nigrodigitatus muscle, is essential to understand the biological, physiological and ecological aspects that may be of advantage in ecotoxicology as a tool for biomonitoring the effects of environmental pollution, as well as food safety. This study was aimed to accomplish a systematic characterization of the muscle proteome as well as to identify a putative set of protein biomarkers in C. nigrodigitatus to environmental pollution in Ologe and Badagry lagoons. Fifteen fi sh samples were used
as representative of the population for proteomics analysis. 116 proteins was expressed with 70 up-regulated, 25 down-regulated from Ologe Lagoon and 30 up-regulated, 17 down-regulated proteins from Badagry Lagoon expressed in the muscle of C. nigrodigitatus in response to environmental stressors using iTRAQ, while 8 protein spots from 2-D gels, representing 8 proteins with 2 up-regulated and 6 down-regulated, have been identifi ed using MALDI TOF/TOF MS. Pearson correlation revealed signifi cant correlation (p < 0.05) betwee environmental variables and protein markers. The investigation revealed that the expressed proteins in the muscle of C. nigrodigitatus served as a prognostic tool to assess the fi sh health and pollution status, which was observed that the fi sh were physiologically perturbed by environmental stressors in Ologe and Badagry lagoons.
Biochemical Monitoring of Detoxifying Enzyme Levels in Field Population of Mo...BRNSS Publication Hub
The major cause of resistance mechanism in mosquitoes is the detoxification and degradation of
insecticides by overproduction of various metabolic enzymes. Quantitative metabolic enzyme assays
of carboxylesterases (α and β), mixed function oxidases (MFO), and glutathione S-transferases (GST)
have been commonly used in the detection of insecticide resistance due to its sensitive nature even at low
frequencies. For the present study, larval strains of Culex quinquefasciatus Say and Aedes aegypti (L) were
collected from the Cochin Corporation, Kerala, India, and were assayed to organophosphate temephos
and carbamate propoxur. The resistance ratio of median lethal time for temephos and propoxur from the
field population of C. quinquefasciatus and A. aegypti is higher than the laboratory population. Elevated
levels of α and β esterase enzyme were observed with the ratio of 1.6 and 1.54 for C. quinquefasciatus
and 1.51 and 1.47 for A. aegypti. In Culex mosquitoes, 1.71, and in Aedes, 1.64 fold increase in GST
enzyme level and 1.38 and 1.3 fold increase for the MFO level determined. The study results revealed
the urgent needs of improving the vector control methods by introducing alternative techniques and
strategies against mosquitoes.
Assimilation Of Phosphite By A. Stolonifera L. And Its In Vitro Effect On Mic...JJDempsey
Abstract on the research into the assimilation and accumulation of phosphite in A. stolonifera and the in vitro effect phosphite has on the mycelial growth of M. nivale
biological activity of Calotropis procera against desert locust and migratory...Narimene Kaidi
The treatments based on acetone extracts of Calotropis procera against imagos of Schistocerca gregaria and Locusta migratoria have been
very convincing due to the speed and efficiency of the action of said plant, on mortality and on haemogram of treated locusts.
My talk at BASF Science Symposium: sustainable food chain - from field to table, Jun 23-24, 2015, Chicago.
Notes and acknowledgements at http://kamounlab.tumblr.com/post/122151022390/plant-pathology-in-the-post-genomics-era
My talk to the PhD students NRP at the Doctoral Training Programme Summer Conference 2015, The Assembly House, Norwich, Thursday 18th June.
Notes and acknowledgments at http://kamounlab.tumblr.com/post/121748816600/what-are-world-class-science-outputs
The influence of reduced oxygen availability on gene expression in laboratory...Santhi Devasundaram
Virtually all dormant
models against tuberculosis tested in animals used laboratory strain H37Rv or Erdman strain. But major
outbreaks of tuberculosis (TB) occur with the strains that have widely different genotypes and phenotypes
compared to H37Rv. In this study, we used a custom oligonucleotide microarray to determine the overall
transcriptional response of laboratory strain (H37Rv) and most prevalent clinical strains (S7 and S10) of
M. tuberculosis from South India to hypoxia.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Rice is one of the most important cereal crops of developing countries and the staple food of about 65% of the world’s population. The rice crops have been greatly disturbed by the heavy metals. The present study deals with the toxic effect of sodium arsenate on morphological and molecular variation through SDS-PAGE in 10 rice (Oryza sativa L.) varieties. Ten varieties of rice were grown under different concentration (25 ppm, 50 ppm and 100 ppm) of sodium arsenate against control. Morphological parameters like shoot length, root length, leaf area and biomass showed marked differences among ten rice varieties. The proteins were separated through SDS-PAGE gel electrophoresis and calculated their molecular weight. The morphological and molecular variations induced in rice varieties by arsenic stress provide a new insight leading to a better understanding of the heavy metal response in plants.
Article Citation:
John De Britto R, Mary Sujin R, Steena Roshan Sebastian and Dharmar K.
Toxic effect of arsenic on ten rice varieties.
Journal of Research in Agriculture (2011) 1(1): 011-016.
Full Text:
http://www.jagri.info/documents/AG0003.pdf
Biochemical Monitoring of Detoxifying Enzyme Levels in Field Population of Mo...BRNSS Publication Hub
The major cause of resistance mechanism in mosquitoes is the detoxification and degradation of
insecticides by overproduction of various metabolic enzymes. Quantitative metabolic enzyme assays
of carboxylesterases (α and β), mixed function oxidases (MFO), and glutathione S-transferases (GST)
have been commonly used in the detection of insecticide resistance due to its sensitive nature even at low
frequencies. For the present study, larval strains of Culex quinquefasciatus Say and Aedes aegypti (L) were
collected from the Cochin Corporation, Kerala, India, and were assayed to organophosphate temephos
and carbamate propoxur. The resistance ratio of median lethal time for temephos and propoxur from the
field population of C. quinquefasciatus and A. aegypti is higher than the laboratory population. Elevated
levels of α and β esterase enzyme were observed with the ratio of 1.6 and 1.54 for C. quinquefasciatus
and 1.51 and 1.47 for A. aegypti. In Culex mosquitoes, 1.71, and in Aedes, 1.64 fold increase in GST
enzyme level and 1.38 and 1.3 fold increase for the MFO level determined. The study results revealed
the urgent needs of improving the vector control methods by introducing alternative techniques and
strategies against mosquitoes.
Assimilation Of Phosphite By A. Stolonifera L. And Its In Vitro Effect On Mic...JJDempsey
Abstract on the research into the assimilation and accumulation of phosphite in A. stolonifera and the in vitro effect phosphite has on the mycelial growth of M. nivale
biological activity of Calotropis procera against desert locust and migratory...Narimene Kaidi
The treatments based on acetone extracts of Calotropis procera against imagos of Schistocerca gregaria and Locusta migratoria have been
very convincing due to the speed and efficiency of the action of said plant, on mortality and on haemogram of treated locusts.
My talk at BASF Science Symposium: sustainable food chain - from field to table, Jun 23-24, 2015, Chicago.
Notes and acknowledgements at http://kamounlab.tumblr.com/post/122151022390/plant-pathology-in-the-post-genomics-era
My talk to the PhD students NRP at the Doctoral Training Programme Summer Conference 2015, The Assembly House, Norwich, Thursday 18th June.
Notes and acknowledgments at http://kamounlab.tumblr.com/post/121748816600/what-are-world-class-science-outputs
The influence of reduced oxygen availability on gene expression in laboratory...Santhi Devasundaram
Virtually all dormant
models against tuberculosis tested in animals used laboratory strain H37Rv or Erdman strain. But major
outbreaks of tuberculosis (TB) occur with the strains that have widely different genotypes and phenotypes
compared to H37Rv. In this study, we used a custom oligonucleotide microarray to determine the overall
transcriptional response of laboratory strain (H37Rv) and most prevalent clinical strains (S7 and S10) of
M. tuberculosis from South India to hypoxia.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Rice is one of the most important cereal crops of developing countries and the staple food of about 65% of the world’s population. The rice crops have been greatly disturbed by the heavy metals. The present study deals with the toxic effect of sodium arsenate on morphological and molecular variation through SDS-PAGE in 10 rice (Oryza sativa L.) varieties. Ten varieties of rice were grown under different concentration (25 ppm, 50 ppm and 100 ppm) of sodium arsenate against control. Morphological parameters like shoot length, root length, leaf area and biomass showed marked differences among ten rice varieties. The proteins were separated through SDS-PAGE gel electrophoresis and calculated their molecular weight. The morphological and molecular variations induced in rice varieties by arsenic stress provide a new insight leading to a better understanding of the heavy metal response in plants.
Article Citation:
John De Britto R, Mary Sujin R, Steena Roshan Sebastian and Dharmar K.
Toxic effect of arsenic on ten rice varieties.
Journal of Research in Agriculture (2011) 1(1): 011-016.
Full Text:
http://www.jagri.info/documents/AG0003.pdf
Rice is one of the most important cereal crops of developing countries and
the staple food of about 65% of the world’s population. The rice crops have been
greatly disturbed by the heavy metals. The present study deals with the toxic effect of
sodium arsenate on morphological and molecular variation through SDS
-
PAGE in 10
rice (
Oryza sativa
L.) varieties. Ten varieties of rice were grown under different
concentration (25 ppm, 50 ppm and 100 ppm) of sodium arsenate against control.
Morphological parameters like shoot length, root length, leaf area and biomass
showed marked differences among ten rice varieties. The proteins were separated
through SDS
-
PAGE gel electrophoresis and calculated their molecular weight. The
morphological and molecular variations induced in rice varieties by arsenic stress
provide a new insight leading to a better understanding of the heavy metal response
in plants.
4 16s rna partial sequencing of isolated strains of metal resistant bacteria ...BIOLOGICAL FORUM
ABSTRACT: Elaeocarpus is a diverse genus within the family Elaeocarpaceae. There is wide distribution of Elaeocarpus in the world among the tropical and subtropical climatic zones. In India, rudraksha (Elaeocarpus sphaericus) has important medicinal and religious values and its history dates back to ancient times. However, the evolutionary relationship of rudraksha with other species of Elaeocarpus is not much explored specially at the molecular and phylogenetic level. The present study establishes evolutionary relationship between rudraksha and other species of Elaeocarpus through phylogenetic algorithms like neighbor joining and maximum likelihood. Thirty species of Elaeocarpus found in the Indo-Australian region were grouped into clusters based on the rDNA and ITS sequence based phylogenetic analysis. This studies paves a way for further studies on evolutionary history of rudraksha with respect to other species of Elaeocarpus and their geographical distribution.
Characterization of Arsenic contaminated Rice (Oryza Sativa L.) through RAPD ...IOSR Journals
Rice being cultivated under anaerobic condition is vulnerable to arsenite, a mobile arsenic speciation Both arsenate and arsenite are highly toxic to human body. Experiments were conducted in Nonaghata (Nadia) and De-ganga (North 24 parganas) with 40 genotypes of rice in Boro season. A few varieties have been identified with low arsenic. Simultaneously some of them were characterized at molecular level by RAPD technique. It is worth to be seen how the low grain and high grain arsenic varieties behave in RAPD fingerprinting. Fourteen out of the 18 decamer random primers showed amplification of genomic DNA in all individuals. A total of 59 fragments were scored, of these fragments, 10 (16.94%) were common to all accessions, 49 (83.06%) were polymorphic and shared by at least eight accessions. It is interesting to mention that the genotypes Azucena and Lemont have already been identified as low grain arsenic genotypes and occupied a distinct different cluster for all primers at the molecular level also. More research is needed in arsenic research in crop plants in different agro climatic situation to have a meaningful and stable conclusion so that the farmers and also the people of West Bengal do not suffer from arsenic hazards at least from the consumption of rice.
Mutagenic and genotoxic assessment of atrazine-based herbicide to freshwater ...Taqprimer institute
Mutagenic and genotoxic assessment of atrazine-based
herbicide to freshwater fishChanna punctatus(Bloch) using
micronucleus test and single cell gel electrophoresis
The objectives of this topic are to understand, acquire, and demonstrate the concept of nanomineral synthesis, their absorption in the body, and effect on livestock productivity.
Genetic variability and phylogenetic relationships studies of Aegilops L. usi...Innspub Net
Studying of genetic relationships among Aegilops L. species is very important for broadening the cultivated wheat genepool, and monitoring genetic erosion, because the genus Aegilops includes the wild relatives of cultivated wheat which contain numerous unique alleles that are absent in modern wheat cultivars and it can contribute to broaden the genetic base of wheat and improve yield, quality and resistance to biotic and abiotic stresses of wheat. The use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in plant biotechnology and their genetics studies. There are different types of markers, morphological, biochemical and DNA based molecular markers. These DNA-based markers based on PCR (RAPD, AFLP, SSR, ISSR, IRAP), amongst others, the microsatellite DNA marker has been the most widely used, due to its easy use by simple PCR, followed by a denaturing gel electrophoresis for allele size determination, and to the high degree of information provided by its large number of alleles per locus. Day by day development of such new and specific types of markers makes their importance in understanding the genomic variability and the diversity between the same as well as different species of the plants. In this review, we will discuss about genetic variability and phylogenetic relationships studies of Aegilops L. using some molecular markers, with theirs Advantages, and disadvantages.
Copper is an essential metal for normal plant growth and development, although it is also potentially toxic. Copper participates in numerous physiological processes and is an essential cofactor for many metalloproteins, however, problems arise when excess copper is present in cells. Excess copper inhibits plant growth and impairs important cellular processes (i.e., photosynthetic electron transport).The mechanisms involved in the acquisition of this essential micronutrient have not been clearly defined although a number of genes have recently been identified which encode potential copper transporters. The present investigation is an attempt to understand of the copper toxicity and tolerance in rice cultivars, and to compare the ten rice cultivars of Karimganj district of Assam. Copper caused growth reduction in rice and among the screened cultivars Amu Sali seems to be a copper sensitive cultivar.
Article Citation:
Upadhyaya H, Bhattacharjee MK, Deboshree Roy, Soumitra Shome.
Toxic effect of copper on ten rice cultivars.
Journal of Research in Plant Sciences (2011) 1(1): 038-044.
Full Text:
http://www.plantsciences.co.in/documents/PS0011.pdf
Ultrastructural and physiological aspects of eucalyptus clones submitted to b...Innspub Net
This work aimed evaluate ultrastructural and physiological aspects of 35-days old eucalyptus, clones 13; 59 and 131, by submitting them to the following barium doses: null, 125; 250; 500 and 1000 ppm of barium solution, with four repetitions. Due to the exposition to barium, there was appearance of phytotoxicity on the leaves. Then, it was determined the following variables: Phloem of Diameter of Leaf and Root (PDL and PDR); Xylem of Diameter of Leaf and Root (XDL and XDR) and Thickness of Palisade Parenchyma (PP); Spad Index (SPADI); Inferior Face of Stomata Functionality of the Inferior Face (IFSF) and Stomach Density of the Inferior Face (SD). Barium negatively influenced the leaf ultrastructure of eucalyptus and its physiology. Eucalyptus clones 13; 59 and 131 are susceptible to barium solution. Barium caused root and ultrastructural leaves and root damages on eucalyptus clones. Xylem diameter of leaves of eucalyptus clones 13 and 59 were not harmed as exposed to Barium.
ABSTRACT- In this study, the effect of ZnO and TiO2-NPs on beneficial soil microorganisms and their secondary metabolites production was investigated. The antibacterial potential of NPs were determined by growth kinetics of P. aeruginosa, P. fluorescens and B. amyloliquefaciens. Significantly decreased in the cell viability based on optical density measurements were observed upon treatment with increasing concentrations of NPs. While comparing the effect of the different concentrations of the NPs (200 µg/ml) on IAA production by different bacterial strains, ZnO nanoparticles showed greater inhibitory effect than TiO2-NPs on IAA production by bacterial strains. The effect of Nanoparticles on phosphate solubilization was found inhibitory at 200 µg/ml. Treatment with ZnO showed concentration dependent enhancement in siderophore production by bacteriaby exposure to ZnO-NPs whereas TiO2-NPs showed concentration dependent progressive decline for iron binding siderophore molecules. Reduction in antibiotic production by P. aeruginosa and P. fluorescens was noticed in the presence of ZnO and TiO2 as compared to the control. The fluorescence of NADH released by P. aeruginosa was observed to be quenched in presence of ZnO and TiO2-NPs as compared to control. The present study highlights that the impact of nanoparticles on bacterial strains and the release of plant growth promoting substances by PGPR strains was dose dependent, which gives an idea about the level of toxicity of these nanoparticles in the environment. Therefore, the discharge of nanoparticles in the environment should be carefully monitored so that the loss of both structure and functions of agronomically important microbes could be protected from the toxicity of MO-NPs.
Key-words- MO-NPs, IAA, Phosphate Solubilization, Siderophore, PCA, NADH, ZnO-NPs, TiO2-NPs
Effect of Different Weed Control Practices On Proximate Composition, Nutrient...IOSRJAVS
A field experiment was conducted during 2004 and 2005 cropping seasons to determine effect of different weed control practices on proximate composition, nutrient concentration and nutrient uptake of maize (Zea maysL.) at the Teaching and Research Farm of Ambrose Alli University, Ekpoma, Nigeria. Seven treatments were used for the experiment, viz no weeding (control), Primextra (3.0kg a.i./ha), mulching (wood shavings), one- hoe weeding at 3 weeks after planting (WAP), two hoe- weedings at 3 and 7 WAP, cover cropping with melon minus hoe-weeding, cover-cropping with melon plus one hoe-weeding at 3WAP. The seven treatments were laid out in randomized complete block design with four replicates. Results showed that mulched plot with wood shavings had the lowest weed density and highest proximate composition, grain nutrient concentration, ear leaf concentration and nutrient uptake followed by Primextra treated plot .The study recommends that small holder farmers to adopt mulching technique with wood shaving to improve the nutritional quality of maize grain since it does not involve any technical rigor besides signifying a non-chemical weed control.
Physiological Quality of Bean Seeds Related To Azotobacter spp. InoculationIOSRJAVS
Research aimed at improving the quality of crops. The results obtained with seed coating were very influential with regard to fertilization and disease resistance. Nitrogen fertilization increases costs in agricultural production and the loss of fertile lands, altering the natural conditions and has negative consequences for the microorganisms (MO), which regulate the balance between quality of crops and soils. The biological nitrogen fixation (BNF) consists on MO work which provide nitrogen in soil and secrete substances that promote plant growth, these can be made by inoculation of seeds. The germination rate, emergency and vigor are an indicator that determines the productivity and physiological quality of a plant variety. The germination rate, number of emerged seeds per pot in favorable field conditions and vigor by accelerated aging test were evaluated. The seed treatments were: the seed inoculation with Azotobacter spp., Immersion in nutrient broth without bacteria, sterilization and zero handling. The experiment has shown that inoculation of common bean seeds with Azotobacter spp. does not adversely affect germination, emergency or vigor and stimulate the development of abnormal seedlings.
Prevalence and Antibiotic sensitivity pattern of Salmonella isolates from mil...IOSRJAVS
The study was conducted to determine the presence of Salmonella in retail milk products and water reservoirs in Maiduguri, Nigeria. A total of 150 samples were collected using convenient sampling technique. One hundred samples; fifty each of full cream milk or Kindirmo (n=50) and Skimmed milk or Nono (n=50) were collected from Bulumkutu and Monday market retail sellers. Furthermore, of the fifty samples obtained from the two different locations, twenty five samples each of kindirmo and nono were collected. The samples were aseptically kept in sterile plastic bags. Additionally, fifty water samples were collected from reservoirs within the University campus. They were processed according to standard bacteriological protocols followed by Gram’s staining and biochemical test; Triple sugar iron test, citrate and urease. The Salmonella isolates were further subjected to ten different antibiotics to determine their sensitivity. The overall prevalence of Salmonella in milk samples was found to be 10.00%, while the total prevalence of Salmonella from water sample was 40.00%. Of the fifteen isolates tested, resistance to Amoxicillin, Ceftriaxone and Erythromycin was 100.00%, Gentamicin had 80.00% and Cotrimoxazole was 53.33%, whereas Gentamycin, Ceftriaxone and Amoxicillin displayed 100.00% sensitivity. Moderate sensitivity of 53.33% to Streptomycin and Pefloxacin, 46.67% to Ciprofloxacin and 86.67% sensitivity to Ofloxacin was found in this study. Antibiotic resistance is associated with frequent usage both in livestock and humans as they are commonly available. Adequate sanitary measures should be ensured in milk processing and use of water reservoirs. Antibiotics should be used based on their antibiogram pattern. Prudent use of antibiotics is essential and its continuous use as growth promoters should be discouraged, as this may result to failure in the treatment of Salmonella-associated diseases due to resistance. Disease surveillance programmes should be established as a means for curtailing salmonellosis.
Molecular Detection and Therapeutic Management of Feline MycoplasmosisIOSRJAVS
Mycoplasma haemofelis and Candidatus Mycoplasma haemominutum (formerly known as Haemobartonellafelis) are the causes of hemotropic mycoplasmosis in cats. The parasites attach to the surface of the red blood cell, and have the potential to cause severe alterations of the cell’s shape, resulting in anaemia. A three-year-old tom cat was presented in University Veterinary Hospital with symptoms of lethargy, reduced appetite and fever for past 3 days. Clinical examination revealed increased temperature(103º – 105º F), blanched mucous membranes and lymphadenopathy. Upon peripheral blood smear examination small coccoid organisms could be noticed in the periphery of the RBCs. Hematobiochemical examination revealed anaemia, thrombocytopaenia and decreased haematocrit values. The blood samples were subjected to DNA extraction and followed by Polymerase Chain Reaction which confirmed mycoplasmosis due to Mycoplasma haemofelis. The animal was treated with intravenous administration of oxytetracycline@ 10mg/kg BW for five days along with prednisolone and vitamin supplements. Uneventful clinical recovery was noticed 7 days post therapy.
Inspection of the imported food products and internally produced food product...IOSRJAVS
This research had done on Export and Internal products which use by Afghan people. Tests on eggs showed that the internal eggs have better condition because of good weight, shell size, Albumin and yolk, and also there is no report of Salmonella. Tests on milk showed that the internal milk is good at high percentage of fat, Protein, Carbohydrates but there is always adulteration in these milks like adding water and starch. The research on water showed that there are hardness, No3, No2, P, some kinds of Bacteria but no Arsenic in tap, well and mineral water.In meat test we had found that, both import frozen meat and daily slaughter had the same condition of total bacteria and Coliforms but the percentage was normal, with no Salmonella. Results showed that import apple had high Bricks in refract meter than Herati apple.Tests of sausage and tuna products of our country and the external samples had no coliforms but high percentage of total bacteria.
Agro-Economic Benefits of Weed Biomass and Crop Residue in Maize Production S...IOSRJAVS
The climatic conditions of coastal Kenya favour rapid weed growth, leading to the accumulation of large biomass of weeds between cropping seasons. Smallholder farmers in the region usually slash and remove the weed biomass and crop residue from their farms during land preparation in order to facilitate easy planting. The impact of such practice on the production of maize has not been assessed. The aim of this study was to determine the effect of the farmers’ practice on the performance of maize and fertilizer requirement. Three methods of managing weed biomass and crop residue (removal from field, incorporation into soil, or use as surface mulch) and five fertilizer rates (60 kg N ha-1 , 20 kg P ha-1 , 30 kg N ha-1 , 10 kg P ha-1 , and no fertilizer application) were evaluated. Removal of weed biomass and crop residue from the field led to 20-26% loss in grain yield and reduced the returns to labour by 41-51%.There was no response to applied P where weed biomass and crop residue had been incorporated into soil. Efforts should therefore be made to educate farmers on the advantages of retaining weed biomass and crop residue on their farms as they prepare land for subsequent crops.
Mutagenic Action of Sodium Azide on Germination and Emergence in Landraces of...IOSRJAVS
Phaseolus vulgaris (Common beans) landraces are one of the most cultivated legumes for human consumption on the montaneclimate of the Jos Plateau and is renowned for the production of reasonable quantities of the beans in Africa.Common beans is rich in fiber, micronutrients, minerals and has strong medicinal value. Despite its potential for improving nutrition and poverty reduction, it is highly under-utilized and research efforts to improve the genotypes of the plant found in this area for better productivity are few. A study was thus carried out to access the germination and emergence responses of three landraces of Phaseolus vulgaris (pinto, red kidney and navy) after treatment with sodium azide. Four doses of sodium azide were applied in concentrations of 0.1M, 0.04M, 0.03M and 0.02M for each landrace for both germination and emergence experiments. The results showed that at doses 0.02M and 0.03M, the pinto indicated significantly (p<0.05) higher mean germination which surpassed the control. All sodium azide doses in the red kidney and pinto had reduced mean germination. Sodium azide reduced the mean emergence of pinto, red kidney and navy at all the doses of sodium azide. The lethality of sodium azide was highest in navy and lowest in pinto. The decrease in germination and emergence was not dose related especially in red kidney and navy. The best response to the mutagen was observed in pinto with higher germination and the least percentage lethality. Sodium azide effectiveness and efficiency were strongest at the 0.1M dose of the mutagen.
The Use of in vitro Gas Production Technique as an Index of the Nutritive Val...IOSRJAVS
: The in vitro gas production technique was used to predict rumen fermentable organic matter, gas production kinetics, organic matter digestibility as well as metabolizable energy were evaluated in green shoot, leaves, fruits flesh and seed cakes of Ziziphus spina-christi tree. A gas production was measured by incubating samples in buffered rumen fluid from cannulated steer for 72 h. Total gas production was recorded at 0,3, 6, 9, 12, 24, 48, and 72 h of incubation periods and kinetics of gas production was described. The chemical analysis of these Ziziphus spina-christi parts, showed that, Leaves contained high protein (14.77±0.23g/kg) and green shoot less protein (8.03±0.15g/kg),however, high ash content was observed in green shoot (10.03±0.07g/kg) compare to other parts. seed cakes has the highest crude fibre content in comparison to other parts (32.46±0.01),while fruits flesh contained the highest ether extract(72.39±0.03).The maximum gas volume was highest for fruits flesh followed by seed cakes, green shoot and leaves after 24hr of incubation. In this study flesh had a significantly higher (P<0.05) gas production from rapid soluble fraction (a) than the other parts, while Ziziphus spina-christi leaves showed the highest gas production from slowly degradable fraction (b) .Organic matter digestibility range was (51.90– 43.79%) and Metabolisable energy was found to be (9.16– 6.74MJ/kgDM) in the flesh and green shoot, respectively. It was concluded that, green shoots, leaves, fruit flesh and seed cake of Ziziphus spina-christi have the potential to be used as protein, energy and mineral supplements for ruminants especially during the dry season
Epithelial reticular Cells of Egyptian Water buffalo (Bosbubalis)IOSRJAVS
Thymus obtained from10 clinically healthy Egyptian water male buffalo (Bosbubalis). Their ages ranged between 2to 3 years old. The thymus of Egyptian water buffalo was divided by septa into many lobules; each lobule had outer, cortex and inner medulla. The epithelial reticular cells were classified according to their staining affinity, morphology and position into 4 types, three were found within the cortex and one in the medulla. In cortex epithelia reticular cells type I (ER 1) had elongated nucleus of irregular or triangular shape with marginal heterochromatin clumps along the nuclear membrane. Epithelial reticular cells type II(ER II) were irregular in shape with pale stained cytoplasm and the nuclei were large and spherical having euchromatin. Epithial reticular cells type III(ERIII) were characterized by large nucleus of irregular shape having marginal heterochromatin .In medulla epithelial reticular (ER) cells of type IV, they were the largest epithelial cells forming the unique elements of the thymic medulla (Hassall's corpuscles)). Cytoplasm was occupied by many small vacuoles ,very large vacuole appeared in the cytoplasm pushed the nucleus into one side.
The Development Strategy for Community Empowerment in the Framework of Fire P...IOSRJAVS
Land and forest fire disasters occur every year. Such conditions lead to disaster smog that many negative impact on society. Limited access to information community, especially around the area of Coconut Plantation potentially cause many problems, such as reduced household income caused by smog, the highaffected community respiratory tract infections as well as conflict disharmony social relationships that exist in society. Mistakes in communicating an information, could lead to uncertainty which aggravated the situation. In this situation, communication and community empowerment approach is urgently needed, taking into account the circumstances and social change. This study aims to examine how socioeconomic conditions of communities around oil palm plantations and the extent of the role of community institutions in the activities of prevention and suppression of fires and haze, and determine the communication strategy of community empowerment in the prevention and suppression of fires and smoke haze oil palm plantations in South Kalimantan, Indonesia. the results showed that the alternative chosen strategy is as follows: (a) Improving socialization, campaigns and the effectiveness of inter-institutional communication for the prevention of forest fires; optimization of the institutional role of Fire Response Group at the grassroots level and improve the knowledge and skills of the community in the management of land resources in order to prevent fires.
Use of Information Communication Technologies Among Female Block Extension Ag...IOSRJAVS
Block Extension Agents are the extension workers who are specifically females and who carry out Gender sensitive issues for female farmers in Nigeria. They have the objective of seeing to the extension needs of the female farmers. The study assessed the use of Information Communication Technologies (ICTs) among Block Extension Agents (BEAs) in South-East Agro-Ecological Zone of Nigeria. Multi-stage sampling technique was used in the selection of the sample for the study. Data were collected with the aid of a structured questionnaire. The data was analysed using descriptive statistics. The mean age of the respondents was 40 years. All the BEAs sampled had formal education where more than half of them (51.04%) had first degree (B.Sc/B.Agric). The mean working experience of the respondents was 10 years. The mean income level of the respondents was N 33,875 per month. The extension agents claimed that twenty-five ICT facilities were available to them in varying degree. The attitude of the respondents towards the use of ICT facilities was positive (mean score=3.11). The extent of use of ICTs by the BEAs was low (mean score=1.45) which can be attributed to the low degree of availability of the ICTs than to their attitude towards the use of the ICT facilities. The BEAs identified thirteen (13) pressing problems that hindered their use of ICTs in varying degree and their major constraint (89.58%) as very limited access to the internet. It is recommended that the ADPs should provide such desirable working conditions that will encourage the BEAs to put in more years of their productive service to the ADPs; Workshops, conferences and seminars should be organized to train the BEAs in the use of ICTs which will improve efficiency in their work. ICT facilities should be made available to BEAs to enhance their productivity.
Study of Genotypic and Phenotypic Correlation among 20 Accessions of Nigerian...IOSRJAVS
Morphological techniques were used to evaluate the diversity in 20 cowpea accessions collected from some parts of Nigeria for two years (2007 and 2008) at Ibadan, South Western Nigeria. Correlation analysis was employed to show the relationships among the traits. Similarly, genotypic and phenotypic variances, genotypic coefficients of variation, heritability and expected genetic advance were estimated for the twelve traits in cowpea for each season. This study shows that for cowpea yield improvement, number of main branches, pod numbers, pods per plant, pods per peduncle and seeds per pod should be part of the selection criteria.
Analysis of Resource Use Efficiency in Small-Scale Maize Production in Tafawa...IOSRJAVS
This paper analyzed the resource-use efficiency of small-scale Maize production in Tafawa-Balewa local government area of Bauchi State. Data were collected from a sample of 120 Maize farmers selected through multi-stage sampling procedure using questionnaire and analyzed using simple descriptive statistics, double-log function and marginal value productivity analysis. The result showed that 90.17% had formal education; 51.67% were males; 90.17% were between the ages of 21-50. Majority 72.50% were married. In terms of farming experience, majority (86.67%) of the respondent had farming experience between 5-20 years. 75.00% had no contact with extension. The double-log function gave the best fit with Adjusted R2 of 81.16%. Production inputs such as seed, fertilizer, labour affected output significantly. Maize production in the study area has an increasing return to scale from the sum of elasticity of production (1.747). Seed and fertilizer were underutilized in Maize production, whereas labour was over used. The major problem confronting the farmers include high cost of inputs (77.50%); Untimely disbursement of credit/inputs (62.50; inadequate extension services (59.17); unstable price (41.67%); draught (33.33%), inadequate credit facilities (31.67%) etc. Profit could be enhanced by increasing the quantity used of seed and fertilizer inputs, its timely supply. Labour should be reduced to optimum level for increase output and total revenue respectively. It is also recommended that extension education and financial support to farmers be improved to allow them increase output and total revenue. There is need for adjustment in resource use in order to improve farm profit at this level of technology used by Maize farmers in the study area.
Role of Trace Minerals in Bolstering Reproductive Health in HeifersIOSRJAVS
Trace minerals play a significant role in improving reproductive health. A study was carried out to evaluate the role of trace minerals in alleviating reproductive health in heifers. A total of 20 heifers were allotted into three groups T0, T1 and T2. T0 (n=4) was kept as control. T1 (n=10) was supplemented with Uniselit (M/S Ayurvet) along with standard basal diet. T2 (n=6) was supplemented with CofeCu along with standard basal diet. Results revealed that there was significant improvement in the physical and chemical parameters of estrus exhibition in the Uniselit supplemented group T1 as compared to control. The conception rate was also higher in group T1 in comparison to control. Thus, it can be inferred that trace minerals have significant influence in improving reproductive health in heifers
Effect of Growth Retardants on Shoot and Root Development of Stevia(Steviareb...IOSRJAVS
Stevia rebaudianaBertoni is a natural sweetener herb, which is promising in food and pharmaceutical production. In Egypt, the gap between sugar production and consumption represents a serious problem, in order to close this gap; Stevia could be cultivated in the Egyptian environmental conditions. The objective of this study was to evaluate the effect of paclobutrazol(PBZ) and ethephon (ET) on shooting and rooting of stevia plant in vitroto improve the survival and growth of Stevia plantlets during acclimatization.The highest shoot number (48.0 shoots/explant) and the highest number of leaves (7.34) were recorded on MS medium containing 0.1mg/l PBZ. Whereas, the highest shoot length (7.36 cm) was obtained on MS medium without supplementation withPBZ or ET. The highest fresh and dry weights of shoots was recorded on MS medium containing 0.5 mg/l PBZ, which gave2.83 and 0.39 g, respectively. The highest number of roots (8.44 roots/shoot) was obtained with 0.1mg/l PBZ. Also, this study indicates that PBZ or ET increased chlorophyll a, chlorophyll b, carotenoids and carbohydrates content in the plant. PBZ and ET had significant effect on thesurvival of plants ex vitro, which recorded 85 and75%, respectively.
Effect of Different Levels of Fat on the Chemical Composition and Sensory Cha...IOSRJAVS
The effect of different levels of fat on the chemical composition and sensory characteristics has been made from the following levels of milk fat: 3%, 2.5% and 2%. The produced cheese was packed into sterile polyethylene bags (300 gram capacity) and stored at 4°C for three months. The chemical analysis was done at 0, 30, 60, 90 and 120 days. The results revealed that significant differences (P< 0.05) were found in protein, ash, total solid, pH, volatile fatty acids and fat contents of the different cheese samples. Sensory characteristics and storage periodof the cheese samples showed significant variations (P< 0.05). The protein, total solids and ash increased throughout the storage period, the lowest values were 21.82±3.49%, 59.29±4.03% and 5.60±0.89% respectively obtained at day zero of the storage. The highest pH occurred at day zero of storage. It was also found that the volatile fatty acids(VFA) increased with the decrease levels of fat, the highest VFA (2.62±0.50%01N ml NaOH/100 gm) was observed in the cheese with 2.5% fat. The statistical analysis showed sensory characteristics of the cheese samples significant (P< 0.05) affected by the fat levels. The present study concluded that production of low-fat cheeses with valuable chemical composition and good quality for the health benefits of the consumers.
Physicochemical and Sensory Characteristics of Sudanese LowFat Cheese during ...IOSRJAVS
This study was carried out in the milk processing units at College of Animal Production Science and Technology, Sudan University of Sciences during September 2014-January 2015. The chemical and sensory characteristics of Sudanese low-fat cheese were investigated as affected by different levels of gum Arabic and storage period. Three hundred and fifty liters (350) of standardized milk with (3%, 2.5% and 2%) of fat were prepared after purchase full cream milk from a private farm at Al Haj Yousif, Khartoum North. Seven treatments were carried out as follows: First treatment is the control in which cheese milk had no additive (3%) of fat. In the second and third with 3% of fat, fourth and fifth with 2.5% of fat and sixth and seventh with 2% of fat, 0.5 and 0.75% of gum Arabic were added respectively to cheese milk before pasteurization then low-fat cheese was made and stored at refrigerator (4°C) for 120 days. Physicochemical and sensory evaluation was done for the cheese samples at day zero, 30, 60, 90 and 120 days intervals. The cheese statistical analysis obtained that gum Arabic significantly (P< 0.05) affected the chemical composition of the cheese. The results also showed that crude protein fat, total solids, pH, volatile fatty acid and ash were significantly (P< 0.05) affected by both levels of gum Arabic and storage period. The results obtained there were significantly differences (P<0.05) between control cheese and the other with 0.5 and 0.75 gum Arabic respectively in all parameters
Assessmens of the Disease Index and the Behavior of Some Peach and Nectarine ...IOSRJAVS
Leaf curl of nectarine and peach, caused by Taphrina deformans (Berk.) Tul, is a difficult disease to control throughout Albania. Heavily infected fruit trees are poorly nourished, develop fewer flower buds, and are more easily damaged from freezing in winter. In this research, susceptibility of 2 peach and 2 nectarine varieties to leaf curl disease was investigated in ecological conditions of Tirane, Albania province in 2014 and 2016. Disease severity was calculated by the 0-5 scale when typical symptoms of leaf curl were evaluated on peach and nectarine leaves in naturally infected orchards. The conditions of natural infection allowed grouping the biological material in 4 classes (plant growth stages) and 6 groups of resistance for leaf curl disease severity. From the data we see that the of disease index (Imc-ja in%) during the three years of study varies from 4.22% in cultivar tire and reaches 22.8% in the Red Star cultivar we also comparisons this level too Control high infection which ranges from 40.66% in Roajal Black cultivar and up to 43.66% in Gomea cultivar.
Characterization and Geographical Classification of Greek Fir Honeys Based on...IOSRJAVS
The objective of the present study was to characterize commercial fir honey produced in different regions in Greece and investigate the possibility of geographical classification using data sets of physicochemical parameters, colour attributes, and volatile compounds using multivariate analysis techniques. For this purpose, 43 fir honey samples were collected during harvesting periods 2010-2013 from five different regions in Greece. Physicochemical parameters (electrical conductivity, ash, pH, moisture, free acidity, lactonic acidity, total acidity, lactonic to free acidity ratio) were determined using official methods of analysis, while colour attributes (L*, a*, b*) and browning index were determined using CIE recommendations and methodology published in the literature, respectively. Finally, volatile profiles were determined using headspace solid phase microextraction coupled to gas chromatography/mass spectrometry. Results showed that honey samples analyzed met the criteria set by regulatory standards for fir honey, and exhibited variations in physicochemical parameter values, colour attributes, and volatile profiles, according to geographical origin. On the basis of the results obtained, it is possible to classify (correct prediction 69.8%) commercial fir honeys produced in specific regions, using a data set of 21 parameters and chemometrics
Impact of Maize Silks Extract, its Application Methods and Their Interaction ...IOSRJAVS
The current study was conducted during the growing season (2014 – 2015), at a private orchard at Safwan area in Basra province-Iraq; to study the effect of several concentrations of maize silks, their application methods and their interaction on some indicators of growth and yield of tomato plants cv. Wijdan. The experiment was factorial and included (24) factorial treatments (4*2*3) resulted from: four levels of maize silks and two application methods, each in three replicates. The results showed a superiority of (60 ml. L.-1) of maize silks extract on most studied characteristics, which was non-significantly differed from the concentration (40 ml. L.-1). For the methods of application, the applying to soil method was superior with most characteristics. Concerning the interaction, the treatment (60 ml. L.-1 maize extract level + applying to soil method) was superior in its effect on most characteristics of tomato plant cv. Wijdan.
Hybrid optimization of pumped hydro system and solar- Engr. Abdul-Azeez.pdffxintegritypublishin
Advancements in technology unveil a myriad of electrical and electronic breakthroughs geared towards efficiently harnessing limited resources to meet human energy demands. The optimization of hybrid solar PV panels and pumped hydro energy supply systems plays a pivotal role in utilizing natural resources effectively. This initiative not only benefits humanity but also fosters environmental sustainability. The study investigated the design optimization of these hybrid systems, focusing on understanding solar radiation patterns, identifying geographical influences on solar radiation, formulating a mathematical model for system optimization, and determining the optimal configuration of PV panels and pumped hydro storage. Through a comparative analysis approach and eight weeks of data collection, the study addressed key research questions related to solar radiation patterns and optimal system design. The findings highlighted regions with heightened solar radiation levels, showcasing substantial potential for power generation and emphasizing the system's efficiency. Optimizing system design significantly boosted power generation, promoted renewable energy utilization, and enhanced energy storage capacity. The study underscored the benefits of optimizing hybrid solar PV panels and pumped hydro energy supply systems for sustainable energy usage. Optimizing the design of solar PV panels and pumped hydro energy supply systems as examined across diverse climatic conditions in a developing country, not only enhances power generation but also improves the integration of renewable energy sources and boosts energy storage capacities, particularly beneficial for less economically prosperous regions. Additionally, the study provides valuable insights for advancing energy research in economically viable areas. Recommendations included conducting site-specific assessments, utilizing advanced modeling tools, implementing regular maintenance protocols, and enhancing communication among system components.
Cosmetic shop management system project report.pdfKamal Acharya
Buying new cosmetic products is difficult. It can even be scary for those who have sensitive skin and are prone to skin trouble. The information needed to alleviate this problem is on the back of each product, but it's thought to interpret those ingredient lists unless you have a background in chemistry.
Instead of buying and hoping for the best, we can use data science to help us predict which products may be good fits for us. It includes various function programs to do the above mentioned tasks.
Data file handling has been effectively used in the program.
The automated cosmetic shop management system should deal with the automation of general workflow and administration process of the shop. The main processes of the system focus on customer's request where the system is able to search the most appropriate products and deliver it to the customers. It should help the employees to quickly identify the list of cosmetic product that have reached the minimum quantity and also keep a track of expired date for each cosmetic product. It should help the employees to find the rack number in which the product is placed.It is also Faster and more efficient way.
Courier management system project report.pdfKamal Acharya
It is now-a-days very important for the people to send or receive articles like imported furniture, electronic items, gifts, business goods and the like. People depend vastly on different transport systems which mostly use the manual way of receiving and delivering the articles. There is no way to track the articles till they are received and there is no way to let the customer know what happened in transit, once he booked some articles. In such a situation, we need a system which completely computerizes the cargo activities including time to time tracking of the articles sent. This need is fulfilled by Courier Management System software which is online software for the cargo management people that enables them to receive the goods from a source and send them to a required destination and track their status from time to time.
Vaccine management system project report documentation..pdfKamal Acharya
The Division of Vaccine and Immunization is facing increasing difficulty monitoring vaccines and other commodities distribution once they have been distributed from the national stores. With the introduction of new vaccines, more challenges have been anticipated with this additions posing serious threat to the already over strained vaccine supply chain system in Kenya.
About
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
• Remote control: Parallel or serial interface.
• Compatible with MAFI CCR system.
• Compatible with IDM8000 CCR.
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
• Easy in configuration using DIP switches.
Technical Specifications
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
Key Features
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
• Remote control: Parallel or serial interface
• Compatible with MAFI CCR system
• Copatiable with IDM8000 CCR
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
Application
• Remote control: Parallel or serial interface.
• Compatible with MAFI CCR system.
• Compatible with IDM8000 CCR.
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
• Easy in configuration using DIP switches.
Industrial Training at Shahjalal Fertilizer Company Limited (SFCL)MdTanvirMahtab2
This presentation is about the working procedure of Shahjalal Fertilizer Company Limited (SFCL). A Govt. owned Company of Bangladesh Chemical Industries Corporation under Ministry of Industries.
Welcome to WIPAC Monthly the magazine brought to you by the LinkedIn Group Water Industry Process Automation & Control.
In this month's edition, along with this month's industry news to celebrate the 13 years since the group was created we have articles including
A case study of the used of Advanced Process Control at the Wastewater Treatment works at Lleida in Spain
A look back on an article on smart wastewater networks in order to see how the industry has measured up in the interim around the adoption of Digital Transformation in the Water Industry.
Overview of the fundamental roles in Hydropower generation and the components involved in wider Electrical Engineering.
This paper presents the design and construction of hydroelectric dams from the hydrologist’s survey of the valley before construction, all aspects and involved disciplines, fluid dynamics, structural engineering, generation and mains frequency regulation to the very transmission of power through the network in the United Kingdom.
Author: Robbie Edward Sayers
Collaborators and co editors: Charlie Sims and Connor Healey.
(C) 2024 Robbie E. Sayers
Explore the innovative world of trenchless pipe repair with our comprehensive guide, "The Benefits and Techniques of Trenchless Pipe Repair." This document delves into the modern methods of repairing underground pipes without the need for extensive excavation, highlighting the numerous advantages and the latest techniques used in the industry.
Learn about the cost savings, reduced environmental impact, and minimal disruption associated with trenchless technology. Discover detailed explanations of popular techniques such as pipe bursting, cured-in-place pipe (CIPP) lining, and directional drilling. Understand how these methods can be applied to various types of infrastructure, from residential plumbing to large-scale municipal systems.
Ideal for homeowners, contractors, engineers, and anyone interested in modern plumbing solutions, this guide provides valuable insights into why trenchless pipe repair is becoming the preferred choice for pipe rehabilitation. Stay informed about the latest advancements and best practices in the field.
Student information management system project report ii.pdfKamal Acharya
Our project explains about the student management. This project mainly explains the various actions related to student details. This project shows some ease in adding, editing and deleting the student details. It also provides a less time consuming process for viewing, adding, editing and deleting the marks of the students.
Halogenation process of chemical process industries
Impact of heavy metals pollution on molecular genetics of some medicinal plants
1. IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS)
e-ISSN: 2319-2380, p-ISSN: 2319-2372. Volume 10, Issue 1 Ver. I (January. 2017), PP 66-71
www.iosrjournals.org
DOI: 10.9790/2380-1001016671 www.iosrjournals.org 66 | Page
Impact of heavy metals pollution on molecular genetics of some
medicinal plants
Esraa. A. El Sherbeny1
A.A. Morsy2
and A.H. Hassan1
1
Desert Research Center, Ministry of Agriculture, Mataria, Cairo, Egypt
2
Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Abstract: Heavy metals are natural constituents of the environment, but indiscriminate use for human purposes
has altered their biochemical and molecular genetic balance. Prolonged exposure and higher accumulation of
such heavy metals can have deleterious health effects on human life. Impact of heavy metals pollution may be
effect on plant in the DNA molecular genetics level. In the present investigation we focus to evaluate the
pollution of heavy metals among three plant species from two sites of polluted and non polluted regions based
on analysis of molecular genetics level of ISSR, AFLP. Five out of the 10 ISSR primers were HB9, HB10, HB11,
HB12 and HB14 which were succeed to amplify 172 reproducible and polymorphic bands on the other hand
AFLP analysis also was used depend on pairs of primers EcoR I- ACA and MseI – CTC which provided a total
of 116 bands ranging from 1550 to 154 bp. Molecular genetics ISSR and AFLP markers appeared more
significant differences between polluted and non polluted plants which will provide a new insight for better
understanding of the molecular basis of nutritional stress responses of wild medicinal plants to pollution which
reflect the genetic defense action and reaction against genetically through appearance some bands product on
the transcription and translation level.
Keywords: heavy metals pollution, molecular genetic, ISSR, AFLP and wild plants The present research aimed
to study the effects of metal pollution stress among three wild plants response on two sites of polluted and non
polluted regions and changes in its profile by using molecular analysis, which provides additional information.
I. Introduction
Several studies indicate that grain production worldwide should increase in the coming years to sustain
the food needs of the growing human population. The world population is expected to reach 10 billion by 2050.
Alkaline and acid soils represent one of the very few options for agricultural expansion for example of abiotic
stress. During the two last decades, studies have been conducted to understand the physiological, biochemical
adaptive strategies used by plants to grow in acid and alkaline soils. Only recently, developments in molecular
biology have resulted in identification, isolation and molecular characterization of genes involved in heavy
metal tolerance and nutrient scavenging, which have important implications for improving plant growth by
means of transgenic approaches.
Heavy metal stress also is one of the major abiotic stresses that cause environmental pollution effect on
plants in recent decades. These metals unlike other organic pollutants are not degraded and converted into
harmless compounds via biological processes in plants (Gisbert et al., 2003). Hence, they can persist for a long
time in the environment and entry into plant food chain. A common feature of environmental stress is their
ability for production of toxic oxygen derivatives (Arora et al., 2002). The transport of heavy metals into plant
roots is increased in acid soils. At high concentrations, plant micronutrients such as Fe, Mn, Cu and Zn and
other metals such as Al, Cd, Pb and Ni for example cause phytotoxic effects, which include reduction of root
growth, reduction in chlorophyll content and photosynthesis, inhibition of enzyme activities and damage to
chloroplasts and mitochondria. Thus, many plant species have developed genetic tolerance to survive in natural
metal-rich soils or in those soils subjected to increasing heavy metal pollution. It is common to find species of
Gramineae, Lamiaceae, Fabaceae and Brassicaceae widely distributed throughout heavy-metalcontaminated
ecosystems worldwide Herrera-Estrella et al., (1999).
In the present study, we report the application of ISSR markers to the study of plant species with
populations adapted to heavy metals, this technique allowed us to show that adaptation to metal-contaminated
soils in populations could be associated with a significant reduction in genetic variation Mengoni et al. (2001).
On the other hand AFLP markers also play effective role for the molecular genetic analysis for polluted and non
polluted plants sites Karp et al. (1997) who reported that AFLP technique is rapidly becoming the method of
choice for estimating genetic diversity in both cultivated and natural populations.
2. Impact of heavy metals pollution on molecular genetics of some medicinal plants
DOI: 10.9790/2380-1001016671 www.iosrjournals.org 67 | Page
II. Materials and methods
Plant materials
Three plant species were used in our study under pollution of heavy metals of cement factory, Alexandria, Borg
El Arab and their replicate under non polluted plants.
LocationFamilyScientific name
Alexandria, Borg El Arab,
Latitude: North, 30°52'52.59
Longitude: East, 29°27'56.96
ChenopodioideaeAtrplix halimus
SalsoloideaeSalsola tetrandum
AsteraceaeEchinops spinosus
Methods
DNA extraction
Extraction of total DNA was performed using methods for medicinal and aromatic plants according to Anna et
al. (2001). To remove RNA contamination, RNase A (10 mg/ml, Sigma, USA) was added to the DNA solution
and incubated at 37°C for 30 min. Estimation of the DNA concentration in different samples was done by
measuring optical density at 260 nm according to the following equation:
Conc. (ug/ml) = OD260 × 50× dilution factor.
Inter simple sequence repeats (ISSRs)
Microsatellite DNA, (simple sequences repeats) which consists of short tandem repeats units 3 to 5 bp
in length that repeats. They also had known as variable number of tandem repeats. Microsatellite polymorphism
is based on the different numbers of a short repeated motif at a given locus SSRs are becoming the markers of
choice in many plant-breeding programs because they are transferable, multiallelic co-dominant markers, PCR-
based, easily reproducible, randomly and widely distributed along the genome, and they can be analyzed
efficiently by the polymerase chain reaction (PCR), using primers specific to their flanking regions variation in
(PCR) product length is a function of the number of SSR units. SSRs have been recommended as standard
markers maps.The ISSR marker is simple and has reproducibility. It requires small amounts of DNA and does
not require information on DNA sequence. ISSR primers are designed from SSR motifs and can be undertaken
for any plant species containing a sufficient number and distribution of SSR motifs in the genome (Gupta et al.,
1996; Buhulikar et al., 2004).
Amplified fragment length polymorphisms (AFLPs) AFLP is a technique based on the detection of genomic
restriction fragments by PCR amplification and can be used for DNA of any origin or complexity. The
fingerprints are produced, without any prior knowledge of sequence, using a limited set of genetic primers.
AFLP allows for the selective amplification of restriction fragments from a total digest of genomic DNA and is
extremely useful in detection of polymorphism between closely related genotypes.
AFLP procedure was applied according to (Vos et al., 1995). Samples were prepared by cutting the genomic
DNA with two restriction enzymes (EcoRI and MseI) and ligating with double stranded EcoR 1and Mse1
adaptors. The adaptors were ligated with the overhanging sticky ends produced by the restriction enzymes.
Statistical data analysis
The distinct and reproducible molecular data bands for both of ISSR and AFLP were scored as either
present (1) or absent (0). The binary data (matrix) was used for calculating Jaccard’s coefficient of genetic
similarity between all possible pairs of collections. A dendrogram (cluster diagram) was constructed with
similarity coefficient using the un weighted pair-group arithmetic averages (UPGMA) procedure of the
NTSYSpc program, version 2.1. The confidence limits for the dendrogram groupings were performed by
bootstrapping using the Win Boot programme (Yap and Nelson, 1996).
III. Results and Discussion
Optimization of ISSR-PCR system and primers screening
Five out of the 10 ISSR primers amplified reproducible and polymorphic bands which used to study the
pollution effect on the molecular level, the results are summarized in (Table 1). The five primers were HB9,
HB10, HB11, HB12 and HB14 which succeed to amplified 172 bands of distinct molecular sizes, the number of
bands per primer ranged from 22 to 31 with an average of 13 bands per primer. A representative ISSR banding
pattern amplified by the primers is presented in Fig 1, also the ISSR molecular data has been revealed some
molecular markers bands in contaminated plants and absence in the other non polluted plants which reflect also
the molecular genetics action against the heave metal pollution. SPSS computer program was applied. The
degree of similarity of ISSR data was represented by similar values, which ranged between 0-1 for the most
dissimilarity and most similar between any given pair of species. To perform and draw the phylogenetic
dendrogram SPSS program was applied. The similarity index ranged from 0.32 to 1.0 with a mean similarity of
0.81, indicating considerable variability detected by ISSR markers. The lowest similarity was observed between
1 and 6 (32%), and the highest similarity was observed between 1 and 2 with 91% similarity which was the most
3. Impact of heavy metals pollution on molecular genetics of some medicinal plants
DOI: 10.9790/2380-1001016671 www.iosrjournals.org 68 | Page
closely related. The result of the dendrogram was similar although it was observed that two distinct clusters
were formed. The polymorphism in presence/absence of ISSR fragments was used to construct a dendogram
(Fig 2) based on Jaccard’s similarity coefficient. In the present analysis of samples, two major clusters were
identified, the first included Atrplix halimus, while the latter included two clusters; one for Salsola tetrandum,
while the other for Echinops spinosus.
Identification of AFLP markers
AFLP analysis using pairs of primers EcoR I- ACA and MseI – CTC provided a total of 116 bands
ranging from 1550 to 154 bp (Figure 3). 79 out of 116 bands were polymorphic and 17 of them were
monomorphic bands.The pair-wise differences among the polluted three sites and their replicate of non polluted
plants were calculated by statistical package for social science (SPSS ver.10) computer program. The analysis
was based on the number of markers that can differentiate between any given pair of genotypes or species to
calculate the similarity matrix.
Dendrogram SPSS program evaluation
SPSS computer program was applied to calculate the degree of similarity which was represented by similar
values, which ranged between 0-1 for the most dissimilarity and most similar between any given pair of species.
To perform and draw the phylogenetic
Sites 3 and 4 were found to be linked together and have closer relationship (84%) with each other within
polluted and non polluted plants. While, sites 1 and 5 were found to be linked together and have not closer
relationship (19%) with each other within polluted and non polluted plants, (Table 3).
Dendrogram was constructed using UPGMA clustering to show the relationships among/between tha two sites
of the polluted and non polluted plants (Figure 4). Analysis of genetic similarity within polluted and non
polluted plants, ranged from 0.1 to 1.0.
The similarity index ranged from 0.19 to 1.0, indicating considerable variability detected by AFLP
markers. The lowest similarity was observed between 1 and 5 (19%) and the highest similarity was observed
between 3 and 4 with 84% similarity which was the most closely related. The result of the dendrogram was
similar although it was observed among the two distinct clusters of ISSR and AFLP which were formed. The
polymorphism in presence/absence of AFLP fragments was used to construct a dendogram (Fig 5) based on
Jaccard’s similarity coefficient. In the present analysis of samples, two major clusters were identified, the first
included Atrplix halimus, while the latter included two clusters one for Salsola tetrandum while the other for
Echinops spinosus.
IV. Conclusion
Molecular genetics studies should complement the morphological and proteomic approaches
concerning the effect of heavy metals stress in genetic expression for increased plant resistance to metal stress.
The results indicated that the application of ISSR markers to the study of plant species with populations adapted
to heavy metals, this technique allowed us to show that adaptation to heavy metal-contaminated in populations
could be associated with a significant reduction in genetic variation, this is consistent with Mengoni et al. (2001)
chloroplast microsatellite analysis showing a large reduction in genetic diversity associated with Silene
paradoxa populations growing in copper-contaminated soils also agreed with Pharmawati et al. (2005) who was
reported that 17 out of 584 ISSR markers were found to be cultivar–specific markers among 30 Leucadendron
cultivars. In addition, it was also agreed with Abdel-Tawab et al. (2007) showed that 48 out of 164 ISSR
markers were specific markers among seven genotypes of Ocimum species using 10 ISSR primers. In general,
the ISSR-PCR was efficient in discriminating among the polluted and non polluted plants under investigation.
On the other hand, results indicated that AFLP markers gave also adequate distinctions among the
polluted and non polluted plants sites, these results agreed also with those Karp et al. (1997) who reported that
AFLP technique is rapidly becoming the method of choice for estimating genetic diversity in both cultivated and
natural populations. Neqi et al. (2000) also used AFLP markers to study inter- and intraspecific genetic
variations using 35 individuals of Withania somnifera and five individuals of W. coagulans which is an
important wild medicinal plants, they suggested that AFLP marker is as an efficient tool for estimating genetic
similarity in plant species and effective management of genetic resources among the polluted and non polluted
plants sites.Bands of DNA markers on the molecular genetics level were successfully to identify the pollution
effect based on the techniques of molecular ISSR and AFLP which were succeed to have great relevance on
taxonomic studies for pollution stress.Conservationist may use the genetics molecular markers bands
information of the present study to make effective decisions regarding the global protection and management
from polluted and non polluted plants in Egypt, which lead us to understand and conclude the genetics
translation defense against pollution by increasing transcription and translation rate during pollution stress
through coding some resist genes against pollution action as defense pathway.
4. Impact of heavy metals pollution on molecular genetics of some medicinal plants
DOI: 10.9790/2380-1001016671 www.iosrjournals.org 69 | Page
In general, the appropriate timeframe for sampling genetic diversity to detect meaningful changes will
vary according to species and to the environment (Gupta et al., 1996). Most of population sites were mining
areas well known for the severity of environmental degradation, it is established that the long lived the species
and the longer the time to reproductive maturity, the longer it will take in absolute time to detect genetic
changes in such environment therefore it was expected that heavy metal tolerant herbaceous species are likely to
exhibit changes in gene expression than conifer tree populations growing in contaminated areas.In finally,
monitoring the genetic diversity of populations has been useful in detecting trends that should alert ecologists to
potential problems of large loss in diversity such as in Cobalt areas could have negative effects on fitness and
survival in long term and the ability to changing environmental conditions.
Table (1): ISSR primer data and the percentage of polymorphic bands.
Figure (1). ISSR electrophoresis patterns of the primers HB9, HB10, HB11, HB12 and HB14. Lane M DNA
marker; lanes 1-6 are identified in Table 1.
Figure (2). Dendrogram based on (UPGMA) method between polluted and non polluted plants based on ISSR
data.
Primer 5’-3’ sequence Number of
monomorphic
bands
Number of
unique
bands
Number of
polymorphic
bands
Total bands
amplified
Polymorphism
(%)
HB9 (GT)6 GG 0 9 13 22 100
HB10 (GA)6 CC 2 3 18 23 91.3
HB11 (GT)6 CC 0 11 20 31 100
HB12 (CAC)3 GC 0 10 19 29 100
HB14 (CTC)3 GC 2 7 16 25 92
5. Impact of heavy metals pollution on molecular genetics of some medicinal plants
DOI: 10.9790/2380-1001016671 www.iosrjournals.org 70 | Page
Figure (3): AFLP profiles between polluted and non polluted plants
Table (2). Similarity matrixes between polluted and non polluted plants based on ISSR data .
1 2 3 4 5 6
1 1.000
2 .916 1.000
3 .506 .519 1.000
4 .485 .404 .832 1.000
5 .383 .422 .513 .589 1.000
6 .323 .344 .526 .585 .850 1.000
Table (3). Similarity matrixes between polluted and non polluted plants. based on AFLP data .
Figure (4). Dendrogram based on (UPGMA) method between polluted and non polluted plants based on AFLP
data.
1 2 3 4 5 6
1 1.000
2 .514 1.000
3 .615 .402 1.000
4 .313 .559 .840 1.000
5 .198 .397 .548 .294 1.000
6 .438 .434 .566 .731 .672 1.000
6. Impact of heavy metals pollution on molecular genetics of some medicinal plants
DOI: 10.9790/2380-1001016671 www.iosrjournals.org 71 | Page
Table (4). Similarity matrixes between polluted and non polluted plant based on ISSR |& AFLP data.
Figure (5). Dendrogram based on (UPGMA) method between polluted and non polluted plants based on ISSR&
AFLP data.
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1 1.000
2 .817 1.000
3 .252 .533 1.000
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