Objective(s):
To evaluate the influences of aqueous extracts of plant parts (stem, leaves, and root) of Portulaca oleracea L. on bioformation of silver nanoparticles (AgNPs).
Materials and Methods:
Synthesis of silver nanoparticles by different plant part extracts of Portulaca oleracea L. was carried out and formation of nanoparticles were confirmed and evaluated using UV-Visible spectroscopy and AFM.
Results:
The plant extracts exposed with silver nitrate showed gradual change in color of the extract from yellow to dark brown. Different silver nanoperticles were formed using extracts of different plant parts.
Conclusion:
It seems that the plant parts differ in their ability to act as a reducing and capping agent.
Enlargement of biologically stimulated
investigational processes for the synthesis of nanoparticles is
budding into an important branch of nanotechnology. Eco
responsive methods of green mediated synthesis of nanoparticles
are the present research in the extremity of nanotechnology. The
bioreduction behavior of leaf extracts of Morinda citrifolia L.
(Rubiaceae) in the green synthesis of silver nanoparticles was
investigated employing UV/Visible Spectrophotometry, Particle
size analyzer, Zeta potential, Filed emission scanning electron
microscopy, Energy Dispersive X-ray Analysis and FourierTransform
Infrared Spectroscopy. The antifungal property of the
silver nanoparticles was tested against Candida albicans,
Candida tropicalis and Candida krusei. The Antifungal assay
tests Zone of inhibition revealed the concentrations of more than
10µl of silver nanoparticles were inhibited the growth of fungal
pathogens.
Term ‘Nano’ comes from the Greek word ‘nanos’ meaning dwarf and denotes a measurement on the scale of one billionth (10⁹) of a meter in size. Nanoparticles are defined as a particulate dispersions of solid particles with atleast one dimension at a size range of 10-1000 nm. The most important feature of Nanoparticles is their surface area to volume aspect ratio, allowing them to interact with other particles easier.
Enlargement of biologically stimulated
investigational processes for the synthesis of nanoparticles is
budding into an important branch of nanotechnology. Eco
responsive methods of green mediated synthesis of nanoparticles
are the present research in the extremity of nanotechnology. The
bioreduction behavior of leaf extracts of Morinda citrifolia L.
(Rubiaceae) in the green synthesis of silver nanoparticles was
investigated employing UV/Visible Spectrophotometry, Particle
size analyzer, Zeta potential, Filed emission scanning electron
microscopy, Energy Dispersive X-ray Analysis and FourierTransform
Infrared Spectroscopy. The antifungal property of the
silver nanoparticles was tested against Candida albicans,
Candida tropicalis and Candida krusei. The Antifungal assay
tests Zone of inhibition revealed the concentrations of more than
10µl of silver nanoparticles were inhibited the growth of fungal
pathogens.
Term ‘Nano’ comes from the Greek word ‘nanos’ meaning dwarf and denotes a measurement on the scale of one billionth (10⁹) of a meter in size. Nanoparticles are defined as a particulate dispersions of solid particles with atleast one dimension at a size range of 10-1000 nm. The most important feature of Nanoparticles is their surface area to volume aspect ratio, allowing them to interact with other particles easier.
Detection of DNA Damage Using Comet Assay Image AnalysisIJRST Journal
Reactive species such as free radicals are constantly produced in vivo and DNA is the very important target of oxidative stress. Oxidative DNA damage is considered as a predictive biomarker to monitor the risk of development of many diseases. The comet assay is widely used for specifying oxidative DNA damage at a single cell level. The analysis of comet assay output images, however, poses considerable challenges. The comet assay, also known as single-cell gel electrophoresis (SCGE), is a simple, sensitive and reliable method for studying DNA damage caused by physical and chemical agents. So the comet assay is a well-established, simple, versatile, visual, rapid, and sensitive method used extensively to assess DNA damage quantitatively and qualitatively at single cell level. The comet assay is most frequently used to analyze white blood cells or lymphocytes in human bio monitoring studies. So through the analysis of comet assay image we can detect edge of damaged DNA comet isolating it from undamaged DNA.
Research Inventy : International Journal of Engineering and Science is published by the group of young academic and industrial researchers with 12 Issues per year. It is an online as well as print version open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as: civil, mechanical, chemical, electronic and computer engineering as well as production and information technology. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published by rapid process within 20 days after acceptance and peer review process takes only 7 days. All articles published in Research Inventy will be peer-reviewed.
Impact of heavy metals pollution on molecular genetics of some medicinal plantsIOSRJAVS
Heavy metals are natural constituents of the environment, but indiscriminate use for human purposes has altered their biochemical and molecular genetic balance. Prolonged exposure and higher accumulation of such heavy metals can have deleterious health effects on human life. Impact of heavy metals pollution may be effect on plant in the DNA molecular genetics level. In the present investigation we focus to evaluate the pollution of heavy metals among three plant species from two sites of polluted and non polluted regions based on analysis of molecular genetics level of ISSR, AFLP. Five out of the 10 ISSR primers were HB9, HB10, HB11, HB12 and HB14 which were succeed to amplify 172 reproducible and polymorphic bands on the other hand AFLP analysis also was used depend on pairs of primers EcoR I- ACA and MseI – CTC which provided a total of 116 bands ranging from 1550 to 154 bp. Molecular genetics ISSR and AFLP markers appeared more significant differences between polluted and non polluted plants which will provide a new insight for better understanding of the molecular basis of nutritional stress responses of wild medicinal plants to pollution which reflect the genetic defense action and reaction against genetically through appearance some bands product on the transcription and translation level.
Seminar presentation entitled 'Towards the development of cost-effective and moderate throughput plant phenotyping system' that was formerly presented during Regional Training Course on Mutation Breeding and Efficiency Enhancing Techniques held by International Atomic Energy Agency (IAEA) 10-20 VI 2014 (Seibersdorf, Austria). Enjoy & share comments!
Biosynthesis and characterization of silver nanoparticles using ficus benghal...eSAT Publishing House
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology
what is radio active isotopes
uses of various fields in tracers
disadvantages of tracers
what are radioactive tracers
application in research fields in tracers
application in agriculture fields
Development of an Automated Comet Assay for Genotoxicity Assessment on TK6 ce...HCS Pharma
Quantifying DNA damage is mandatory to assess potential adverse effects of candidate drugs or molecules or extracts developed in the dermo-cosmetic industry, but also to assess the efficacy of therapeutic approaches with the aim of producing tumor cell genotoxicity in cancer treatment.
The comet assay is a sensitive, well established technique for quantifying DNA damage in eukaryotic cells. Compatible with the detection of a wide range of DNA damaging agents, its principle consists in the migration of fragmented DNA in an electrophoresis gel (damaged DNA forming the tail of the comet), while intact DNA moves at a slower rate (head of the comet). The percentage of fragmented DNA in the comet tail is a direct measure of DNA damage.
Nanoparticle of plant extract: A Novel approach for cancer therapyroshan telrandhe
Presented in NATIONAL CONFERENCE ;A Phytomedicine : A Novel Approach For Cancer Treatment. Sponsered By Indian Councial Of Medical Research, Delhi. OrganisedBy KamlaNehru College Of Pharmacy, Butibori, Nagpur441108, Maharastra, India. ON 25 & 26 March 2017
Detection of DNA Damage Using Comet Assay Image AnalysisIJRST Journal
Reactive species such as free radicals are constantly produced in vivo and DNA is the very important target of oxidative stress. Oxidative DNA damage is considered as a predictive biomarker to monitor the risk of development of many diseases. The comet assay is widely used for specifying oxidative DNA damage at a single cell level. The analysis of comet assay output images, however, poses considerable challenges. The comet assay, also known as single-cell gel electrophoresis (SCGE), is a simple, sensitive and reliable method for studying DNA damage caused by physical and chemical agents. So the comet assay is a well-established, simple, versatile, visual, rapid, and sensitive method used extensively to assess DNA damage quantitatively and qualitatively at single cell level. The comet assay is most frequently used to analyze white blood cells or lymphocytes in human bio monitoring studies. So through the analysis of comet assay image we can detect edge of damaged DNA comet isolating it from undamaged DNA.
Research Inventy : International Journal of Engineering and Science is published by the group of young academic and industrial researchers with 12 Issues per year. It is an online as well as print version open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as: civil, mechanical, chemical, electronic and computer engineering as well as production and information technology. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published by rapid process within 20 days after acceptance and peer review process takes only 7 days. All articles published in Research Inventy will be peer-reviewed.
Impact of heavy metals pollution on molecular genetics of some medicinal plantsIOSRJAVS
Heavy metals are natural constituents of the environment, but indiscriminate use for human purposes has altered their biochemical and molecular genetic balance. Prolonged exposure and higher accumulation of such heavy metals can have deleterious health effects on human life. Impact of heavy metals pollution may be effect on plant in the DNA molecular genetics level. In the present investigation we focus to evaluate the pollution of heavy metals among three plant species from two sites of polluted and non polluted regions based on analysis of molecular genetics level of ISSR, AFLP. Five out of the 10 ISSR primers were HB9, HB10, HB11, HB12 and HB14 which were succeed to amplify 172 reproducible and polymorphic bands on the other hand AFLP analysis also was used depend on pairs of primers EcoR I- ACA and MseI – CTC which provided a total of 116 bands ranging from 1550 to 154 bp. Molecular genetics ISSR and AFLP markers appeared more significant differences between polluted and non polluted plants which will provide a new insight for better understanding of the molecular basis of nutritional stress responses of wild medicinal plants to pollution which reflect the genetic defense action and reaction against genetically through appearance some bands product on the transcription and translation level.
Seminar presentation entitled 'Towards the development of cost-effective and moderate throughput plant phenotyping system' that was formerly presented during Regional Training Course on Mutation Breeding and Efficiency Enhancing Techniques held by International Atomic Energy Agency (IAEA) 10-20 VI 2014 (Seibersdorf, Austria). Enjoy & share comments!
Biosynthesis and characterization of silver nanoparticles using ficus benghal...eSAT Publishing House
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology
what is radio active isotopes
uses of various fields in tracers
disadvantages of tracers
what are radioactive tracers
application in research fields in tracers
application in agriculture fields
Development of an Automated Comet Assay for Genotoxicity Assessment on TK6 ce...HCS Pharma
Quantifying DNA damage is mandatory to assess potential adverse effects of candidate drugs or molecules or extracts developed in the dermo-cosmetic industry, but also to assess the efficacy of therapeutic approaches with the aim of producing tumor cell genotoxicity in cancer treatment.
The comet assay is a sensitive, well established technique for quantifying DNA damage in eukaryotic cells. Compatible with the detection of a wide range of DNA damaging agents, its principle consists in the migration of fragmented DNA in an electrophoresis gel (damaged DNA forming the tail of the comet), while intact DNA moves at a slower rate (head of the comet). The percentage of fragmented DNA in the comet tail is a direct measure of DNA damage.
Nanoparticle of plant extract: A Novel approach for cancer therapyroshan telrandhe
Presented in NATIONAL CONFERENCE ;A Phytomedicine : A Novel Approach For Cancer Treatment. Sponsered By Indian Councial Of Medical Research, Delhi. OrganisedBy KamlaNehru College Of Pharmacy, Butibori, Nagpur441108, Maharastra, India. ON 25 & 26 March 2017
Antimicrobial and cytotoxicity effect of silver nanoparticle synthesized by C...Nanomedicine Journal (NMJ)
Objective(s): For the development of reliable, ecofriendly, less expensive process for the synthesis of silver nanoparticles and to evaluate the bactericidal, and cytotoxicity properties of silver nanoparticles synthesized from root extract of Croton bonplandianum, Baill.
Materials and Methods: The synthesis of silver nanoparticles by plant part of Croton bonplandianum was carried out. The formation of nanoparticles was confirmed by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), XRD and UV-Vis spectrophotometric analysis. The biochemical properties were assayed by antibacterial study, cytotoxicity assay using cancer cell line.
Results: The formation of silver nanoparticles was confirmed by UV-VIS spectroscopic analysis which showed absorbance peak at 425 nm. X-ray diffraction photograph indicated the face centered cubic structure of the synthesized AgNPs. TEM has displayed the different dimensional images of biogenic silver nanoparticles with particle size distribution ranging from 15-40 nm with an average size of 32 nm. Silver particles are spherical in shape, clustered. The EDX analysis was used to identify the elemental composition of synthesized AgNPs. Antibacterial activity of the synthesized AgNPs against three Gram positive and Gram negative bacteria strains like Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa carried out showed significant zones of inhibition. The cytotoxicity study by AgNPS also showed cytotoxicity on ovarian cancer cell line PA-1 and lung epithelial cancer cell line A549.
Conclusion: The present study confirms that the AgNPs have great promise as antibacterial, and anticancer agent.
Presentation from recent LibreOffice training session. Full training also included hands-on practice time and organization-specific documents. For more information, please visit togsolutions.com
- 2-Aminio-5-substituted -1, 3, 4-thiadiazoles
(I) on reaction with formalin in ethanol solvent yielded 2,
2, 2-(1, 3, 5- triazinone- 1, 3, 5-tri-yl)- tris (5-substituted 1,
3, 4-thiadiazoles) (II) in moderate to excellent yields
— In the present work, impact of UV-B radiation (280-
315nm: 0.4 W m-2) on growth, photosynthetic pigments, protein,
ascorbate, proline and lipid peroxidation have been studied in
two cyanobacteria Nostoc muscorum and Synechocystis PCC
6803. UV-B radiation (2 to 6 hrs) leads to 55% inhibition of
growth in Synechocystis PCC 6803 in comparison to control
where as in Nostoc muscorum growth reduces up to 45%. This
UV-B treatment also significantly decreased the contents of
chlorophyll, carotenoids and phycocyanin. Photosynthetic
pigments decreased with increasing doses of UV-B (2 to 6 hrs)
radiation. However, the inhibitory effect in Synechocystis PCC
6803 was more pronounced than in Nostoc muscorum. With
increasing UV-B exposure period, production of ascorbate (19-
45%), proline (12-29%) and lipid peroxidation was significantly
higher in Synechocystis PCC 6803 as compared to control
sample. It was observed that lipid peroxidation enhanced 33 %
than control sample of Synechocystis PCC 6803. Our result shows
that photosynthetic apparatus is the main target of UV-B
radiation causing degradation of photosynthetic pigments. This
study concluded that Synechocystis PCC 6803 was the susceptible
organism for survival in stress condition than Nostoc muscorum.
Green synthesis, Characterization and anti microbial activity of silver nano ...IJERA Editor
The exploitation of various plant materials for the biosynthesis of silver nano particles is considered a green
technology. Because it does not involve any harmful chemicals. Nanotechnology field is one of the most
attractive researches. The field of nanotechnology is applied to bio materials. This review focuses on the green
synthesis of silver nanoparticles using various plant sources. A detailed study on the reduction of silver ions to
silver nanoparticles from medical plant leaves extract were demonstrated with a brief experimental procedure.
Characterization of the synthesized nanoparticles performed through UV spectroscopy, Fourier Transform Infra
Red spectroscopy analysis, X-Ray Diffraction analysis, Scanning Electron Microscopy and Transmission
Electron Microscopy. This review mainly focus on anti microbial activities of synthesized silver nano particles.
Hydroxyl capped silver-gold alloy nanoparticles: characterization and their c...Nanomedicine Journal (NMJ)
Objective(s):
Metal nanoparticles (NPs) offer a wide variety of potential applications in pharmaceutical sciences due to the unique advances in nanotechnology research. In this work, bimetal Ag-Au alloy NPs were prepared and their combinations with other antibiotics were tested against Staphylococcus aureus.
Materials and Methods:
Firstly, Ag-Au alloy NPs with Au/Ag molar ratio of 1:1 was fabricated and was purified by agarose gel electrophoresis system. The morphology and size of the purified NPs were confirmed by transmission electron microscopy. Chemical composition and surface chemistry of these NPs were studied with atomic absorption spectophotometry and Fourier transforms infrared spectroscopy, respectively. The size of purified Ag-Au alloy NPs was less than 200 nm. Also the presence of organic compounds with a hydroxyl residue was detected on the surface of these purified NPs. In next step the effect of purified Ag-Au alloy NPs on the antibacterial activity of different antibiotics was evaluated at sub-inhibitory content (5 μg/disk) using disk diffusion method against S. aureus. Ag NPs and Au NPs were also tested at same content (5 μg) using mentioned method.
Results:
The most enhancing effect of Ag-Au alloy NPs was observed for penicillin G and piperacillin. No enhancing effects on the antibacterial activity of different antibiotics were observed at 5 μg/disk for the mono-metal nanoparticles (Ag NPs and Au NPs) against S. aureus.
Conclusion:
These results signify that the Ag-Au alloy NPs potentiates the antimicrobial action of certain antibiotics suggesting a possible utilization of this nano material in combination therapy against resistant S. aureus.
assessment of biomass of leaves of water hyacinth (eichhornia crassipes)IJAEMSJORNAL
Green chemistry methods for nanoparticles synthesis have implemented the valorization of renewable waste that reduces the use of chemicals and sub-products to minimize the environmental impact. Herein, we report a method to synthesize Ag and Au nanoparticles (AgNPs, AuNPs) using one of the world´s worst aquatic weeds, water hyacinth. From a reaction between a solution of AgNO3 or HAuCl4 and controlling the pH, the nanoparticles were synthesized. The optimum pH value to obtained uniform quantum dots was found to be acidic for AgNPs and neutral for AuNPs. The size was highly dependent on pH for AgNPs, a smaller size was for acidic pH, and the larger size was for basic pH, and cubic and hexagonal are the predominant structures, no dependent was observed in AuNPs, and orthorhombic is the most common form. This method was sustainable because water hyacinth is a renewable resource in all world, and their use is not being exploited in any process. The bioreduction process using water hyacinth promotes the metallic nanoparticles formation and applied standard conditions for temperature and pressure. Also, the rate of synthesis is fast.
Is Nano Medicine And Nano Technology The Most Trending Thing Now?science journals
Nano medicine is nothing but application of Nano technologies as medicines. It may include application of non-material as biological devices or nano-electronic biosensors. Molecular nanotechnology as biological machines may have medical applications in future.
Plant Mediated Synthesis of Silver Nanoparticles by Using Dried Stem Powder o...IJERA Editor
The Tinospora Crispa is an important medicinal plant to synthesize silver Nanoparticles provides environmentally benign and a feasible alternative to the most of the chemical, physical and biological methods. Utilizing the reduced property of Tinospora Crispa dried stem powder from 1mM aqueous silver nitrate, the average size of 40nm silver nanoparticles were synthesized at room temperature. The stem powder extracts mixed with silver nitrate showed a gradual change in the color of the extracts from yellow to dark brown. The formation of silver nanoparticles was confirmed by UV-Visible spectrophotometer, X-Ray diffraction (XRD), Fourier transform infrared (FTIR), Energy dispersive spectroscopy (EDAX) and Transmission electron microscopy (TEM). The photoluminescence studies of silver nanoparticles shows that they are efficient fluorescence emitting probes.
Silver Nanoparticles Synthesis, Properties, Applications and Future Perspecti...iosrjce
Silver nanoparticles (Ag NPs) have gained significant interest due to their unique optical,
antimicrobial, electrical, physical properties and their possible application. The change of energy level from
continuous band to discrete band of Ag NPs with decrease in size of particles gives strong size dependent
chemical and physical properties. Ag NPs show lower toxicity to human health while Ag NPs show higher
toxicity to various micro-organisms. For this reason Ag NPs having scope for medical instruments,
antimicrobial application, products for health care such as scaffolds, burn dressing, water purification,
agriculture uses. Ag NPs can be synthesized by using various methods which is primarily classified into two
type’s namely physical process which includes laser ablation, condensation, evaporation etc. and chemical
process which includes hydrazine, sodium borohydride, green synthesis etc. Among all these methods green
synthesis is non-toxic, eco-friendly and cost effective. In this review paper different synthesis process especially
green synthesis, properties, applications of silver nanoparticles and their recent advances are described. We
also highlight the toxicity and compares Ag NPs with others nanoparticles.
It an overall view on two research papers. Biological synthesis of Nano particles from plants and microorganisms
and the synthesis of metallic Nano particles using plant extract
Here, we report the synthesis of silver nanoparticles (AgNPs) by utilizing extract of Psidium guajava. In
this study leaf was with extricated alcohol, further this extract is utilized for the preparation of nanoparticles followed by treatment with silver nitrate solution. Color change from colorless to dark-yellow and
SPR band (UV-Vis) 420nm which indicates the synthesis of plant reduced silver nanoparticles. The presences of proteins as capping agent, which increase the stability of AgNPs in the colloids, are characterized
by Fourier transform infrared spectroscopy (FTIR). Transmission electron microscopy (TEM) demonstrated the presence at spherical AgNPs in the range of 15-30nm. The bactericidal activity of standard
antibiotics was essentially expanded in the presence of AgNPs against pathogenic bacteria, S Aureus,
Staphylococcus, E Coli, P Putida and fungus A Niger.
Green synthesis of zinc oxide nano particles using flower extract cassia dens...IJERD Editor
Green synthesis of metal nanoparticles is an interesting issue of the nanoscience and
nanobiotechnology. There is a growing attention to biosynthesis the metal nanoparticles using organisms.
Among these organisms, plants seem to be the best and they are suitable for large scale biosynthesis of
nanoparticles. Nanoparticles produced by plants are more stable, and the rate of synthesis is faster than that in
the case of other organisms. The present investigation was carried out to green synthesis of zinc oxide
nanoparticles by using the medicinal plant cassia densistipulata taub. The flower was collected from the campus
of Anantapuramu, Andhra Pradesh and their petals were separated. The petals were taken and cleaned with
dimeneralized water and soaked for an hour on dry cloth to remove moisture from the petals.
Synthesis of Zinc Nanoparticles was done by mixing 5gms of Zinc Nitrate with 50ml of aqueous
extract of cassia densistipulata taub petals. The formation of nanoparticles was monitored by visualizing color
changes and it was confirmed by Electron microscope (SEM), UV-Vis spectrophotometer and Fourier
Transform Infra-Red (FT-IR) spectroscopy. The results of various techniques confirmed the presence Zinc oxide
nanoparticles.
Microwave Assist Green Synthesis of Silver Nano Particles Using Rhynchostylis...Dr. Amarjeet Singh
A novel green approach for the synthesis and
stabilization of silver nanoparticles (AgNPs) using water
extract of Rhynchostylisretusa(L.) Blume leaf has been
developed. As obtained, the nanoparticles are characterized by
UV-visible (UV-Vis), FTIR, X-ray diffraction (XRD) and SEM
analysis. The crystalline nature of the AgNPs is confirmed by
the prominent peaks in the XRD pattern. FTIR spectra suggest
that the possible biomolecules are responsible for the efficient
stabilization of the sample. The prepared nanoparticle shows
good antioxidant activity.
Similar to Synthesis of silver nanoparticle using Portulaca oleracea L. extracts. Article 5, Volume 1, Issue 2, Winter 2014, Page 94-99 (20)
Evaluation of the effect of crocetin on antitumor activity of doxorubicin enc...Nanomedicine Journal (NMJ)
Objective(s): The current study reports investigation of codelivery by PLGA nanoparticles (NPs) loaded with crocetin (Cro), a natural carotenoid dicarboxylicHYPERLINK “http://en.wikipedia.org/wiki/Carboxylic_acid” acid that is found in the crocus flower, and Doxorubicin (DOX).
Materials and Methods: Double emulsion/solvent evaporation method was used for preparation of PLGA nanoparticles containing Dox and Cro. Characterizations of prepared NPs were investigated by atomic force microscopy (AFM) and dynamic light scattering analysis. In vitro Cytotoxicity of DOX and Cro loaded PLGA NPs (PLGA-DOX-Cro) on MCF-7 cell line was evaluated using MTT test. Flow cytometry experiments were implemented to distinguish cells undergoing apoptosis from those undergoing necrosis. Furthermore the expression of caspase 3 was examined by western blot analysis.
Results: The prepared formulations had size of 150- 300 nm. Furthermore, PLGA-DOX-Cro nanoparticles inhibited MCF-7 tumor cells growth more efficiently than either DOX or Cro alone at the same concentrations, as quantified by MTT assay and flow cytometry. Studies on cellular uptake of DOX-Cro-NPs demonstrated that NPs were effectively taken up by MCF-7 tumor cells.
Conclusion: This study suggested that DOX-Cro-NPs may have promising applications in breast cancer therapy.
Effects of combination of magnesium and zinc oxide nanoparticles and heat on ...Nanomedicine Journal (NMJ)
Objective: The objective of this study was to investigate the antibacterial activities of combination of MgO and ZnO nanoparticles in the presence of heat against Escherichia coli and Staphylococcus aureus.
Materials and Methods:Bacteria were grown on either agar or broth media followed by the addition of ZnO and MgO nanoparticles. Then the combined effect of ZnO and MgO nanoparticles was investigated. Furthermore, the media containing nanoparticles were treated with mild heat and their synergistic antibacterial activity was investigated against E. coli and S. aureus in milk.
Results: The data showed that the nanoparticles used in this study had no effect on the bacteria in the agar medium. However, the results showed that ZnO and MgO nanoparticles resulted in a significant decrease in the number of E. coli (P<0.000) and S. aureus (Pd”0.05) in the broth medium. The combination of nanoparticles and mild heat exhibited a significant decrease in the number of E. coli and S. aureus indicating the synergistic effects of nanoparticles and heat.
Conclusion: Using a combination of mild heat, ZnO and MgO nanoparticles, E. coli and S. aureus can be controlled successfully in the milk. Mild heating plus ZnO and MgO nanoparticles has a synergistic effect which would reduce the need for high temperature and also the concentrations of ZnO and MgO nanoparticles required for pathogen control in minimally processed milk during maintaining.
Preparation and evaluation of electrospun nanofibers containing pectin and ti...Nanomedicine Journal (NMJ)
Objective(s):The aim of this study was to prepare electrospun nanofibers of celecoxib using combination of time-dependent polymers with pectin to achieve a colon-specific drug delivery system for celecoxib.
Materials and Methods:Formulations were produced based on two multilevel 22 full factorial designs. The independent variables were the ratio of drug:time-dependent polymer (X1) and the amount of pectin in formulations (X2). Electrospinning process was used for preparation of nanofibers. The spinning solutions were loaded in 5 mL syringes. The feeding rate was fixed by a syringe pump at 2.0 mL/h and a high voltage supply at range 10-18 kV was applied for electrospinning. Electrospun nanofibers were collected and evaluated by scanning electron microscopy and drug release in the acid and buffer with pH 6.8 with and without pectinase.
Results:Electrospun nanofibers of celecoxib with appropriate morphological properties were produced via electrospinning process. Drug release from electrospun nanofibers was very low in the acidic media; while, drug release in the simulated colonic media was the highest from formulations containing pectin.
Conclusion: Formulation F2 (containing drug:ERS with the ratio of 1:2 and 10% pectin) exhibited acceptable morphological characteristics and protection of drug in the upper GI tract and could be a good candidate as a colonic drug delivery system for celecoxib.
The combined effects of Aloe vera gel and silver nanoparticles on wound heali...Nanomedicine Journal (NMJ)
Objective(s): This study was aimed at investigating the synergy effects of Aloe vera gel and silver nanoparticles on the healing rate of the cutting wounds.
Materials and Methods: In order to determine the concentration of silver nanoparticles in Aloe vera gel, the MBC methods were applied on the most common bacteria infecting wounds, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. The cutting wounds with Full-thickness skin were dorsally created on rats; then the rats were divided into 4 groups. The treatments groups included: mixture of Aloe vera gel and silver nanoparticles, Aloe vera gel alone and silver nanoparticles alone in addition to control groups. The treatment was carried out for 2 weeks and the size of the wound closures were measured by an image software analysis.
Results:There was no significant difference (p<0.05) in healing rate between the control and mixture group. However, there were significant differences between the silver nanoparticles and Aloe vera groups using Tukey’s analysis on the 6th, 8th and 10th days.
Conclusion:The Aloe vera gel increased the rate of wound healing whereas the silver nanoparticles had a delay effect; and when they were mixed, it was similar to the average effect of both Aloe vera gel and silver nanoparticles.
Simultaneous loading of 5-florouracil and SPIONs in HSA nanoparticles: Optimi...Nanomedicine Journal (NMJ)
Objective(s): Over the past two decades, considerable interest has been focused on utilizing biocompatible magnetic nanoparticles (MNPs) for biomedical applications. In this study, production of human serum albumin (HSA) nanoparticles using desolvation technique that were simultaneous loaded with high amounts of superparamagnetic iron oxide nanoparticles (SPIONs) and 5-flourouracil (5-FU) was investigated.
Materials and Methods: 5-FU loading (%) and SPIONs entrapment efficiency (%) were optimized using response surface methodology (RSM). The design expert software used to analyse the interactive effects of pH, 5-FU and SPIONs concentrations.
Results:The optimum conditions found to be pH of 8.2, drug concentration of 1.5 mg/ml and SPIONs concentration of 2.79 mg/ml. Under the mentioned optimum conditions, particles with the size of 111.8 nm, zeta potential of -37.1 mV, 5-FU loading of 15.8% and SPIONs entrapment efficiency of 41.1% were obtained. In vitro cumulative release of 5-FU from the nanoparticles was evaluated in phosphate buffer saline (pH 7.4, 37 °C). Results indicated that 85% of the 5-FU released during 95 h, which revealed a sustained release profile. In addition, Vibrating Sample Magnetometer (VSM) analyses confirmed the superparamagnetic properties of magnetic albumin nanoparticles manufactured under the optimum conditions.
Conclusion: According to the findings,SPIONs and 5-FU loaded HAS nanoparticles arepromising for use as novel targeted delivery system due to proper magnetic and drug release behaviours.
Investigation of the effect of different parameters on the phase inversion te...Nanomedicine Journal (NMJ)
Objective(s): Nanoemulsions are a kind of emulsions that can be transparent, translucent (size range 50-200 nm) or “milky” (up to 500 nm). Nanoemulsions are adequatly effective for transfer of active component through skin which facilitate the entrance of the active component . The transparent nature of the system and lack of the thickener and fluidity are among advantages of nanoemulsion.
Materials and Methods: In this study, a nanoemulsion of lemon oil in water was prepared by the phase inversion temperature (PIT) emulsification method in which the tween 40 was used as surfactant. The effect of concentration of NaCl in aqueous phase, pH and weight percent of surfactant and aqueous on the PIT and droplet size were investigated. Results: The results showed that with increasing of concentration of NaCl from 0.05 M to 1 M, PIT decrease from 72 to 50. The average droplet sizes, for 0.1, 0.5 and 1 M of NaCl in 25 ºC are 497.3, 308.1 and 189.9 nm, respectively and the polydispersity indexes are 0.348, 0.334 and 0.307, respectively.
Conclusion: Considering the characteristics of nanoemulsions such as being transparent, endurance of solution and droplet size can provide suitable reaction environment for polymerization process used in making hygienic and medical materials.
Mechanism of oxidative stress involved in the toxicity of ZnO nanoparticles a...Nanomedicine Journal (NMJ)
ZnO NPs (zinc oxide nanoparticles) has generated significant scientific interest as a novel antibacterial and anticancer agent. Since oxidative stress is a critical determinant of ZnO NPs-induced damage, it is necessary to characterize their underlying mode of action. Different structural and physicochemical properties of ZnO NPs such as particle surface, size, shape, crystal structure, chemical position, and presence of metals can lead to changes in biological activities including ROS (reactive oxygen species) production. However, there are some inconsistencies in the literature on the relation between the physicochemical features of ZnO NPs and their plausible oxidative stress mechanism. Herein, the possible oxidative stress mechanism of ZnO NPs was reviewed. This is worthy of further detailed evaluations in order to improve our understanding of vital NPs characteristics governing their toxicity. Therefore, this study focuses on the different reported oxidative stress paradigms induced by ZnO NPs including ROS generated by NPs, oxidative stress due to the NPs-cell interaction, and role of the particle dissolution in the oxidative damage. Also, this study tries to characterize and understand the multiple pathways involved in oxidative stress induced by ZnO NPs. Knowledge about different cellular signaling cascades stimulated by ZnO NPs lead to the better interpretation of the toxic influences induced by the cellular and acellular parameters. Regarding the potential benefits of toxic effects of ZnO NPs, in-depth evaluation of their toxicity mechanism and various effects of these nanoparticles would facilitate their implementation for biomedical applications.
Combined effects of PEGylation and particle size on uptake of PLGA particles ...Nanomedicine Journal (NMJ)
Abstract
Objective:
At the present study, relationship between phagocytosis of PLGA particles and combined effects of particle size and surface PEGylation was investigated.
Materials and Methods:
Microspheres and nanospheres (3500 nm and 700 nm) were prepared from three types of PLGA polymers (non-PEGylated and PEGylation percents of 9% and 15%). These particles were prepared by solvent evaporation method. All particles were labeled with FITC-Albumin. Interaction of particles with J744.A.1 mouse macrophage cells, was evaluated in the absence or presence of 7% of the serum by flowcytometry method.
Results:
The study revealed more phagocytosis of nanospheres. In the presence of the serum, PEGylated particles were phagocytosed less than non-PEGylated particles. For nanospheres, this difference was significant (P<0/05) and their uptake was affected by PEGylation degree. In the case of microsphere formulation, PEGylation did not affect the cell uptake. In the serum-free medium, the bigger particles had more cell uptake rate than smaller ones but the cell uptake rate was not influenced by PEGylation.
Conclusion:
The results indicated that in nanosized particles both size and PEgylation degree could affect the phagocytosis, but in micron sized particles just size, and not the PEGylation degree, could affect this.
Synthesis of silver nanoparticles and its synergistic effects in combination ...Nanomedicine Journal (NMJ)
Abstract
Objectives:
Biofilms are communities of bacteria attached to surfaces through an external polymeric substances matrix. In the meantime, Acinetobacterbaumannii is the predominant species related to nosocomial infections. In the present study, the effect of silver nanoparticles alone and in combination with biocides and imipenem against planktonic and biofilms of A. baumannii was assessed.
Materials and Methods:
Minimum inhibitory concentrations (MICs) of 75 planktonic isolates of A. baumannii were determined by using the microdilution method as described via clinical and laboratory standards institute (CLSI). Among all strains, 10 isolates which formed strong biofilms were selected and exposed to silver nanoparticles alone and in combination with imipenem, bismuth ethandithiol (BisEDT) and bismuth propanedithiol (BisPDT) to determine minimum biofilm inhibitory concentrations (MBIC). Subsequently, minimum biofilm eradication concentrations (MBECs) of silver nanoparticles alone and in combination with imipenem against mature biofilm of the isolates were evaluated.
Results:
Results showed that 29.3% of isolates were susceptible to silver nanoparticles and could inhibit the growth and eradicate biofilms produced by the isolates. For this reason, ∑FIC, ∑FBIC and ∑FBEC ≤ 0.05 were reported which shows synergism between silver nanoparticles and imipenem against not only planktonic cells but also inhibition and eradication of biofilms. The results of ∑FBIC >2 indicated to antagonistic impacts between silver nanoparticles and BisEDT/BisPDT against biofilms.
Conclusion:
It can be concluded that silver nanoparticles alone can inhibit biofilm formation but in combination with imipenem are more effective against A. baumannii in planktonic and biofilm forms.
Abstract
Objective(s):
Zinc oxide nanoparticles (ZNP) are increasingly used in sunscreens, biosensors, food additives and pigments. In this study the effects of ZNP on liver of rats was investigated.
Materials and Methods:
Experimental groups received 5, 50 and 300 mg/kg ZNP respectively for 14 days. Control group received only distilled water. ALT, AST and ALP were considered as biomarkers to indicate hepatotoxicity. Lipid peroxidation (MDA), SOD and GPx were detected for assessment of oxidative stress in liver tissue. Histological studies and TUNEL assay were also done.
Results:
Plasma concentration of zinc (Zn) was significantly increased in 5 mg/kg ZNP-treated rats. Liver concentration of Zn was significantly increased in the 300 mg/kg ZNP-treated animals. Weight of liver was markedly increased in both 5 and 300 mg/kg doses of ZNP. ZNP at the doses of 5 mg/kg induced a significant increase in oxidative stress through the increase in MDA content and a significant decrease in SOD and GPx enzymes activity in the liver tissue. Administration of ZNP at 5 mg/kg induced a significant elevation in plasma AST, ALT and ALP. Histological studies showed that treatment with 5 mg/kg of ZNP caused hepatocytes swelling, which was accompanied by congestion of RBC and accumulation of inflammatory cells. Apoptotic index was also significantly increased in this group. ZNP at the dose of 300 mg/kg had poor hepatotoxicity effect.
Conclusion:
It is concluded that lower doses of ZNP has more hepatotoxic effects on rats, and recommended to use it with caution if there is a hepatological problem.
Synthesis of graphene oxide-TiO2 nanocomposite as an adsorbent for the enrich...Nanomedicine Journal (NMJ)
Abstract
Objective(s):
In our study, graphene oxide-TiO2 nanocomposite (GO/TiO2) was prepared and used for the enrichment of rutin from real samples for the first time.
Materials and Methods:
The synthesized GO/TiO2 was characterized by X-ray diffraction, scanning electron microscopy, and FT-IR spectra. The enrichment process is fast and highly efficient. The factors including contact time, pH, and amount of GO/TiO2 affecting the adsorption process were studied.
Results:
The maximum adsorption capacity for ciprofloxacin was calculated to be 59.5 mg/g according to the Langmuir adsorption isotherm. The method yielded a linear calibration curve in the concentration ranges from 15 to 200 μg/L for the rutin with regression coefficients (r2) of 0.9990. The limits of detection (LODs, S/N=3) and limits of quantification (LOQs, S/N=10) were found to be 8 μg/Land 28 μg/L, respectively. Both the intra-day and inter-day precisions (RSDs) were < 10% .
Conclusion:
The developed approach offered wide linear range, and good reproducibility. Owing to the diverse structures and unique characteristic, GO/TiO2 possesses great potential in the enrichment and analysis of trace rutin in real aqueous samples.
Preparation and evaluation of vitamin A nanosuspension as a novel ocular drug...Nanomedicine Journal (NMJ)
Abstract
Objective(s):
The aim of this study was to prepare a nanosuspension formulation as a new vehicle for the improvement of the ocular delivery of vitamin A.
Material and Methods:
Formulations were designed based on full factorial design. A high pressure homogenization technique was used to produce nanosuspensions. Fifteen formulations were prepared by the use of different combinations of surfactants Tween 80, benzalkonium chloride and Pluronic and evaluated for pH, particle size, entrapment efficiency, differential scanning calorimetry (DSC), stability and drug release. Also, Draize test was used to evaluate the irritation of rabbit eye by formulations.
Results:
All formulations showed a small mean size that is well suited for ocular application. Also it was observed that the particle size decreased with increase in the amount of surfactant. Drug entrapment increased with increasing amount of surfactant. It was shown that initial and final drug release can be controlled by the ratio and the total amount of surfactants, respectively.
Conclusion:
It was concluded that the use of Tween 80 and Pluronic in the formualtions with a proper ratio does not show eye irritation and could be useful to achieve a suitable nanosuspension of vitamin A as a novel ocular delivery system.
A comparative study about toxicity of CdSe quantum dots on reproductive syste...Nanomedicine Journal (NMJ)
Abstract
Objective(s):
Medicinal benefits of quantum dots have been proved in recent years but there is little known about their toxicity especially in vivo toxicity. In order to use quantum dots in medical applications, studies ontheir in vivo toxicity is important.
Materials and Methods:
CdSe:ZnS quantum dots were injected in 10, 20, and 40 mg/kg doses to male mice10 days later, mice were sacrificed and five micron slides were prepared structural and optical properties of quantum dots were evaluated using XRD.
Results:
Histological studies of testis tissue showed high toxic effect of CdSe:ZnS in 40 mg/kg group. Histological studies of epididymis did not show any effect of quantum dots in terms of morphology and tube structure. Mean concentration of LH and testosterone and testis weight showed considerable changes in mice injected with 40 mg/kg dose of CdSe:ZnS compared to control group. However, FSH and body weight did not show any difference with control group.
Conclusion:
Although it has been reported that CdSe is highly protected from the environment by its shell, but this study showed high toxicity for CdSe:ZnS when it is used in vivo which could be suggested that shell could contribute to increased toxicity of quantum dots. Considering lack of any previous study on this subject, our study could potentially be used as an basis for further extensive studies investigating the effects of quantum dots toxicity on development of male sexual system.
Functionalization of carbon nanotubes and its application in nanomedicine: A ...Nanomedicine Journal (NMJ)
Abstract
This review focuses on the latest developments in applications of carbon nanotubes (CNTs) in medicine. A brief history of CNTs and a general introduction to the field are presented.
Then, surface modification of CNTs that makes them ideal for use in medical applications is highlighted. Examples of common applications, including cell penetration, drug delivery, gene delivery and imaging, are given. At the same time, there are concerns about their possible adverse effects on human health, since there is evidence that exposure to CNTs induces toxic effects in experimental models. However, CNTs are not a single substance but a growing family of different materials possibly eliciting different biological responses. As a consequence, the hazards associated with the exposure of humans to the different forms of CNTs may be different. Understanding the structure–toxicity relationships would help towards the assessment of the risk related to these materials. Finally, toxicity of CNTs, are discussed. This review article overviews the most recent applications of CNTs in Nanomedicine, covering the period from 1991 to early 2015.
The role of surface charge of ISCOMATRIX nanoparticles on the type of immune ...Nanomedicine Journal (NMJ)
Abstract
Objective(s):
ISCOMATRIX vaccines have now been shown to induce strong antigen-specific cellular or humoral immune responses to a broad range of antigens of viral, bacterial, parasite or tumor. In the present study, we investigated the role of ISCOMATRIX charge in induction of a Th1 type of immune response and protection against Leishmania major infection in BALB/c mice.
Materials and Methods:
Positively and negatively charged ISCOMATRIX were prepared. BALB/C mice were immunized subcutaneously, three times with 2-week intervals, with different ISCOMATRIX formulations. Soluble Leishmania antigens (SLA) were mixed with ISCOMATRIX right before injection. The extent of protection and type of immune response were studied in different groups of mice.
Results:
The group of mice immunized with negatively charged ISCOMATRIX showed smaller footpad swelling upon challenge with L. major and the highest IgG2a production compared with positively charged one. The mice immunized with positively charged ISCOMATRIX showed the lowest splenic parasite burden compared to the other groups. Cytokine assay results indicated that the highest level of IFN- γ and IL-4 secretion was observed in the splenocytes of mice immunized with negatively charged ISCOMATRIX as compared to other groups.
Conclusion:
The results indicated that ISCOMATRIX formulations generate an immune response with mixed Th1/Th2 response that was not protective against challenge against L. major.
Abstract
In the last decade, developments in nanotechnology have provided a new field in medicine called “Nanomedicine”. Nanomedicine has provided new tools for photodynamic therapy. Quantum dots (QDs) are approximately spherical nanoparticles that have attracted broad attention and have been used in nanomedicine applications. QDs have high molar extinction coefficients and photoluminescence quantum yield, narrow emission spectra, broad absorption, large effective stokes shifts. QDs are more photostable and resistant to metabolic degradation. These photosensitizing properties can be used as photosensitizers for Photodynamic Therapy (PDT). PDT has been recommended for its unique characteristic, such as low side effect and more efficiency. Therefore, nanomedicine leads a promising future for targeted therapy in cancer tumor. Furthermore, QDs have recently been applied in PDT, which will be addressed in this review letter. Also this review letter evaluates key aspects of nano-particulate design and engineering, including the advantage of the nanometer scale size range, biological behavior, and safety profile.
Preparation of protein-loaded PLGA-PVP blend nanoparticles by nanoprecipitati...Nanomedicine Journal (NMJ)
Abstract
Objective(s):
Despite of wide range applications of polymeric nanoparticles in protein delivery, there are some problems for the field of protein entrapment, initial burst and controlled release profile.
Materials and Methods:
In this study, we investigated the influence of some changes in PLGA nanoparticles formulation to improve the initial and controlled release profile. Selected parameters were: pluronic F127, polysorbate 80 as surfactant, pH of inner aqueous phase, L/G ratio of PLGA polymer, volume of inner aqueous phase and addition of polyvinylpyrrolidone as an excipient. FITC-HSA was used as a model hydrophilic drug. The nanoparticles were prepared by nanoprecipitation.
Results:
Initial release of FITC-HSA from PLGA-tween 80 nanoparticles (opt-4, 61%) was faster than control (PLGA-pluronic) after 2.30 h of incubation. Results showed that decrease in pH of inner aqueous phase to pI of protein can decrease IBR but the release profile of protein is the same as control. Release profile with three phases including a) initial burst b) plateau and c) final release phase was observed when we changed volume of inner aqueous phase and L/G ratio in formulation. Co-entrapment of HSA with PVP and pluronic reduced the IBR and controlled release profile in opt-19. Encapsulation efficiency was more than 97% and nanoparticles size and zeta potentials were mono-modal and -18.99 mV, respectively.
Conclusion:
In this research, we optimized a process for preparation of PLGA-PVP-pluronic nanoparticles of diameter less than 300 nm using nanoprecipitation method. This formulation showed a decreased initial burst and long lasting controlled release profile for FITC-HSA as a model drug for proteins.
Abstract
Objective(s):
Abdominal adhesions are one of the most important problems, occurring after intra-abdominal surgery in more than 90% of cases. This condition is the leading cause of bowel obstruction, infertility, and abdominal/pelvic pain. Gold nanoparticles (GNPs) have been shown to be non-toxic and exhibit anti-inflammatory, anti-angiogenic and antioxidant activities. The purpose of this study was to determine the effect of intraperitoneal lavage with GNP solutions on the development of postoperative peritoneal adhesion (PPA).
Materials and Methods:
In the current experimental study, thirty-five male Wistar rats were randomly assigned to seven groups of five rats. After a standardized peritoneal injury, GNP solutions in different concentrations (1, 2.5, 5, 10, 50 and 100 ng/ml) were locally administered through nebulization; normal saline (NS) was administered to the control group. Two weeks later, the rats were sacrificed and cecum and peritoneal samples were harvested for histopathological assessment. Blood samples were obtained to determine serum concentrations of inflammatory biomarkers including tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and vascular endothelial growth factor (VEGF).
Results:
The rats treated with GNPs had significantly lower microscopic and macroscopic peritoneal adhesion scores, compared to the control group (P<0.05). Score 5 of macroscopic adhesions was reported in all the rats of the control group, unlike the GNP groups. Furthermore, microscopic adhesions were reported with all rats in the control group, unlike the GNP groups (reported in 0 out of 5 rats in all GNP groups). In addition, serum levels of IL-1β, TNF-α and VEGF underwent no significant changes.
Conclusion:
Compared to the control group, GNPs decreased the severity of peritoneal adhesions, although they did not alter TNF-α, IL-1β or VEGF serum levels.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
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Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
Synthesis of silver nanoparticle using Portulaca oleracea L. extracts. Article 5, Volume 1, Issue 2, Winter 2014, Page 94-99
1. 94 Nanomed J, Vol. 1, No. 2, Winter 2014
Received: Jun. 15, 2013; Accepted: Sep. 29, 2013
Vol. 1, No. 2, Winter 2014, page 94-99
Online ISSN 2322-5904
http://nmj.mums.ac.ir
Original Research
Synthesis of silver nanoparticle using Portulaca oleracea L. extracts
Asghari Gholamreza1*
, Jaleh Varshosaz2
, Nafiseh Shahbazi1
1
Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
2
Department of Pharmaceutics, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Objective(s): To evaluate the influences of aqueous extracts of plant parts (stem, leaves, and
root) of Portulaca oleracea L. on bioformation of silver nanoparticles (AgNPs).
Materials and Methods: Synthesis of silver nanoparticles by different plant part extracts of
Portulaca oleracea L. was carried out and formation of nanoparticles were confirmed and
evaluated using UV-Visible spectroscopy and AFM.
Results: The plant extracts exposed with silver nitrate showed gradual change in color of the
extract from yellow to dark brown. Different silver nanoperticles were formed using extracts
of different plant parts.
Conclusion: It seems that the plant parts differ in their ability to act as a reducing and
capping agent.
Keywords: Nanoparticles, Plant part, Portulaca oleracea, Silver
*Corresponding authors: Gholamreza Asghari, Isfahan Pharmaceutical Sciences Research Center, Isfahan
University of Medical Sciences, Isfahan, Iran.
Tel: +98 311-7922644, Email:asghari@pharm.mui.ac.ir
2. Silver nanoparticle by Portulaca oleacea
Nanomed J, Vol. 1, No. 2, Winter 2014 95
Introduction
Today, most studies in the field of
nanoparticles (NPs) are conducted on
heavy metals such as Ag, Pt, Pd and Au.
Metal NPs are used extensively in
different areas such as catalyst, biological
detection probes, electronic, and medicine
(1).With the expansion of NPs
applications, new ways for synthesis of
metal NPs are considered among which
NPs of Ag are more efficient, as their anti-
microbial effects play a significant role in
the domain of biology and medicine (2).
There are different methods for the
synthesis of silver NPs, such as reduction
of solvent, chemical and photochemical
reaction in reverse cycles, and thermal
decomposition of Ag components (3).
Most of these methods are dangerous,
because they use toxic and hazardous
solvent or high pressure and temperature,
thus they are harmful for ecology and
natural environment.
Therefore, finding new synthesis methods
for Ag NPs which are harmless for natural
environment and are economic is
necessary.
Expanding processes for synthesizing NPs
which are friendly to the environment
namely using microorganisms, fungus and
plants are also useful for reducing waste
materials on natural environment.
These methods are called green synthesis
of NPs (4). The success of green synthesis
in comparison with other physical and
chemical methods is due to its
compatibility with nature, easy application
and capability of doing synthesis in large
scale, without using high pressure, high
temperature, high energy and poisonous
chemicals (5).
Biosynthesis of metal NPs by plant extract
is the best nature-friendly method.
Application of several plant aerial part
extracts such as alfalfa and Aloe vera are
already reported (6, 7).
It was reported that active biological
compounds in the plant extract such
as proteins, polysaccharides, vitamins and
polyphenols are responsible for reduction
of metal ions to the metal particles (8).
Based on the reported research, polyols
and water soluble heterocyclic complexes
are responsible for the reduction of silver
ions and stability of NPs (8).
In line with the pervious researches on
rapid synthesis of silver nanoparticle by
fresh leaves water extract of porslan
(Portulaca oleracea) from the family of
Portulacaceae (9), different part of dried P.
oleracea was selected for investigating the
formation of silver NPs.
Different part of this plant has different
active compounds, such as phenolic acids,
oxalic, malic, ascorbic acid, soluble
carbohydrates, tannin, omega-3,
flavonoids and alkaloids (10).
Moreover it is rich in proteins, amino
acids, and glycosides.
Each part of the plant is rich source of
compounds which are known as good
reducing agents for silver ion (10). The
influence of plant part extract (stem,
leaves, and root) on particle size of silver
NPs is the aim of this research.
Materials and Methods
Plant part extracts
The P. oleracea seeds were obtained from
Nikanbazr Company, Isfahan, Iran.
The plants were prepared by cultivation of
the seeds and harvested in full flowering
stage.
The plant roots, stems and leaves were
separately dried at room temperature. 5
grams of powdered leaves, stems and roots
were mixed with 100 ml distillated water
and poured in to 300 ml volume flasks.
This mixture was boiled for 5 minutes.
After decantation, solution was kept at
4ºC.
Silver NPs formation with extracts of
Portulaca oleracea L.
10 ml of the extracts of each plant parts
was added into 190 ml of 10-3
M AgNO3.
The formation of Ag NPs was investigated
through monitoring color change of the
3. Asghari G, et al
96 Nanomed J, Vol. 1, No. 2, Winter 2014
solution from yellow to dark brown and
using Nano ZetaSizer and UV- visible
spectrophotometer in wavelength between
350-800 nm (11).
Then AFM was applied for morphological
examination and particles size
measurements (12).
Results
The extract obtained from root, leaves and
stem of dried Portulaca oleracea in the
presence of clear solution of 10-3
M
AgNO3, showed color change from yellow
to dark brown after 1-2 hours at room
temperature.
This seems to indicate the formation Ag
NPs (Figure 1). Reduction of the silver ion
to silver NPs during exposure to the plant
leaf extracts could be followed by color
change and thus UV-vis spectro-scopy.
Figures 2-4 show the analysis of the
particle size of NPs obtained by Nano
ZetaSizer from different parts of the plant.
Figures 5-8 are AFM images of NPs
formed with 5% plant broth and 1 mM
AgNO3 solution.
It is shown that relatively spherical NPs
are formed with average diameter of about
136-175 nm based on plant part used.In
order to screen plant with high production
capability of silver NPs, different plant
part extracts were compared for their
ability to synthesize silver NPs
synthesis.As shown in Figure 2-4, the
particle size formed was the highest for
stem extract and the lowest for leave
extracts.
Discussion
The different plant part extracts of
Portulaca oleracea were able to form silver
NPs with different particle size and shape
in vitro.As presented in Figures 2-7, there
are differences on AgNPs formationability
according to plant part used.
Figure 1. The color changing of leave extract
mixed with AgNO3 solution within 2 hours.
Figure 2. The size of silver nanoparticles obtained
from stem of Portulaca oleracea.L.
Figure 3. The size of silver nano particles obtained
From leaves of Portulaca oleracea.L.
4. Silver nanoparticle by Portulaca oleacea
Nanomed J, Vol. 1, No. 2, Winter 2014 97
It was reported that the fresh leaves
aqueous extract of the plant synthesized
silver nanoparticles with particle size less
than 60 nm (9).
But the dried leaves in current study
produce silver NP with the size of 136 nm
which may related to change in leaves
compositions such as enzymes over
drying.
As presented on Figs. 2-4, the plant part
extract form nanoparticles with different
sizes; 146 nm, 136 nm, and 175 nm for
root, leave, and stem extracts, respectively.
The mechanism of biological synthesis of
NPs is not fully understood.
Figure 5. The AFM images of silver nanoparticles
in leaves extract of Portulaca oleracea.L.
Gold NPs were synthesized
extracellularly by the Tamarindus
indica. It was reported that the reduction
occurred due to release of reductase
enzyme into the solution (13).
Figure 6. The AFM images of silver nanoparticles
in root extract of Portulaca oleracea.L.
Figure 7. The AFM images of silver nanoparticles
in stem extract of Portulaca oleracea.L.
Cinnamomum camphora has also been
revealed to fabricate Au and Ag NPs. It
is believed that terpenoids are active
molecules stabilizing the NPs and
reaction of the metal ions is possibly
facilitated by terpenoids broth (14).
Studies on the fruit extract of Emblica
officinalis indicated that the
proteins played a reducing and
controlling role during the formation
of silver NPs in the solutions (15).
Bioreduction activity of leaf extracts of
Helianthus annus, Basella alba, and
Saccharum officinarum resulted in the
fabrication of Ag NPs in which
Helianthus annus was found to exhibit
strong potential for quick reduction of
Ag ions (16). The polyol components
and the water soluble heterocyclic
components are mainly res-ponsible for
reduction of Ag+ as well as stabilization
of NPs. It seems that the basis of all
metal NPs synthesis methods is the
Figure 4. The size of silver nano particles obtained from
root of Portulaca oleracea.L.
5. Asghari G, et al
98 Nanomed J, Vol. 1, No. 2, Winter 2014
reduction of metal ions by reduction
agents. Portulaca oleracea stem, leave,
and root contains different quantity of
phenols, flavonoids, carbohydrates,
alkaloids, triterpens, phenolic acids,
ascorbic, malic, citric and oxalic acids
(17).
Flavonoids are responsible for antioxidant
activity in plant (18). It seems that water
soluble carbohydrates such as
fructose/froctan, amino acids such as
glotamic acid, alanin, and phenylalanin
which are found differently in plant parts
are responsible for reduction and stability
of Ag NPs (11, 19).
The presence of polyol and soluble
heterocyclic complexes are necessary for
reduction of Ag ions and stability of Ag
NPs (8).
Also quality and quantity of polysacch-
arides exist in different plant parts are
effective factors in biosynthesis and
stability of NPs.
The exact mechanism for the fabrication of
NPs in biological resources is still being
investigated and several possible ways
have been proposed (20, 21).
Several studies still need to be executed to
understand the effect of parameters
regarding the influences of many and
different constituents available in stem,
leave, and root for phytofabrication of
AgNPs by P. oleracea extracts.
Most published date available on the green
Ag NPs biosynthesis ability of plant leaf
extract (22-27), however, there are several
reports on stem, fruit extract as well (15,
28).
Rich compounds occurrence in different
parts of P. oleracea that are able to reduce
Ag ions and help the stability of NPs, short
reaction time, rapid growth of the plant in
different environmental conditions, will
justify the value of using root, leaves, and
stem of the plant.
Meanwhile, different size Ag NPs obtains
is among the beneficial goals of the
present method to evaluate and compare
the sliver biosynthesis ability of each plant
part separately.
Conclusion
Rich compounds occurrence in different
parts of P. oleracea that are able to reduce
Ag ions and help the stability of NPs, short
reaction time, rapid growth of the plant in
different plant parts, will justify the value
of using root, leaves, and stem of the plant.
Meanwhile, different size AgNPs obtains
is among the beneficial goals of the
present method to evaluate and compare
the sliver biosynthesis ability of each plant
part separately.
Acknowledgments
This study was financially supported by
School of Pharmacy, Isfahan University of
Medical Sciences, Isfahan, Iran.
References
1. Song JY, Kim BS. Rapid biological
synthesis of silver nanoparticles using
plant leaf extract. Bioprocess Biosyst Eng.
2009; 32: 79-84.
2. Aleer MK. Tapping the unexploited plant
resources for the synthesis of silver nano-
particles. Afr J Biotechnol. 2008; 7(17):
3162-3165.
3. Nagajyoti PC, Prasad TNVKA, Sreekanth
TVM, Lee KD. Biofabrication of silver
nanoparticles using leaf extract of
Saururus chinenis. Dig J Nanomater Bios.
2011; 69(1): 121-133.
4. Castro-Langoria E,Vilchis-Nestor AR,
Avalos-Borja M. Biosynthesis of silver
and gold and bimetallic nanoparticles
using the filamentous fungus Neurospora
crassa. Colloids Surf B. 2011; 83(1): 42-
48.
5. Raveendran P. Fu J. Wallen SL.
Completely green synthesis and
stabilization of metal nanoparticles. J Am
Chem Soc. 2003; 125(46): 13440-13441.
6. Gardea-Torresdey JL, Gomez E, Peratta-
Videa JR, Parsons JG, Trioani H, Jose-
Yacaman M. Alfalfa sprouts: A natural
source for synthesis of silver
nanoparticles. Langmuir. 2003; 19(4):
1357-1361.
7. Chandran SP, Chaudhary M, Posricha R,
Ahmad A, Sastry M. Synthesis of gold
nanoparticles and silver nanoparticles
using Aloe Vera Plant extract. Biotechnol
Prog. 2006; 22(2):577-583.
8. Velmurugan P, Shim J, Kamala-Kannan S,
Lee KJ, Oh BT, Balachandar V.
Crystallization of silver through reduction
process using Elaeis guineensis biosolid
6. Silver nanoparticle by Portulaca oleacea
Nanomed J, Vol. 1, No. 2, Winter 2014 99
extract. Biotechnol Prog. 2011; 27: 273-
279.
9. Firdouse MJ, Lalitha P., Green
biosynthesis of silver nanoparticles using
the aqueous extract of Portulaca oleracea
(L.). Asian J Pharm Clin Res. 2013; 6(1):
92-96.
10. Sanja SD, NR. Sheth, NR, Patel NP, Patel
D, Patel B. Characterization and
evaluation of antioxidant activity of
Portulaca oleracea L. Int J Pharm Pharm
Sci. 2009; 1(1): 5-10.
11. Rajasekharreddy P, Rani PU, Sreedhar B.
Qualitative assessment of silver and gold
nanoparticle synthesis in various plants: a
photobiological approach. J Nanopart Res.
2010; (12): 1711- 1721.
12. Vilchis AR. Sandrez-Mendieta NC,
Camacho-Lopez, MA. Gomez-Espinosa
RM. , Camacha-Lopez MA, Arenas-
Alatarre JS. Solventless synthesis and
optical properties of Au and Ag
nanoparticles using Camellia sinensis
extract. Mater Lett. 2008; 62: 3103-3105.
13. Ankamwar B, Chaudhary M, Mural S.
Gold Nanotriangles biologically
synthesized using tamarind leaf extract
and potential application in vapor sensing.
Synth React Inorg Met -Org Chem. 2005;
35: 19-26.
14. Huang J, Li Q, Sun D, Lu Y, Su Y, Yang
X, Wang H, Wang Y, Shao W, He N,
Hong J, Chen C, Biosynthesis of silver
and gold nanoparticles by novel sundried
Cinnamomum camphora leaf.
Nanotechnology. 2007; 18: 105-115.
15. Ankamwar B, Damle C, Ahmad A, Sastry
M. Biosynthesis of gold and silver
nanoparticles using Emblica officinalis
fruit extract, their phase transfer and
transmetallation in an organic solution. J
Nanosci Nanotechnol. 2005; 5: 1665-
1671.
16. Leela A, Vivekanandan M. Tapping the
unexploited plant resources for the
synthesis of silver nanoparticles. Afr J
Biotechnol. 2008; 7: 3162-3165.
17. Liu L, Howe P, Zhou YF, Xu ZQ, Hocart
C, Zhang R. Fatty acids and β-Carotene in
Australian Purslane (Portulaca oleracea)
varieties. J Chromatogr A. 2000; 893 (1):
207-213.
18. Sakai N, Inada K, Okamoto M, Shizuri Y,
Fukuyama Y. Portuloside A, A
monoterpene glucoside, from Portulaca
oleracea. Phytochemistry. 1996; 42(6):
1625-1628.
19. Mohamed A. Hussein AS. Chemical
composition of purslane (Portulaca
oleracea). Plant Food Hum Nutr. 1994;
45(1): 1-9.
20. Shankar SS, Ahmad A, Sastry M,.
Geranium leaf assisted biosynthesis of
silver nanoparticles. Biotechnol Prog.
2003; 19: 1627-1631.
21. Rai M, Yadav A, Gade A, Current trends
in photosynthesis of metal nanoparticles.
Crit Rev Biotechnol. 2008; 28: 277- 284.
22. Sathyavathi R, Krishna MB, Rao SV,
Saritha R, Rao DN. Biosynthesis of silver
nanoparticles using Coriandrum sativum
leaf extract and their application in
nonlinear optics. Adv Sci Lett. 2010;
3(2): 1- 6.
23. Mallikarjuna K, Narasimha G, Dillip GR,
Praveen B, Shreedhar B, Lakshmi CS,
Reddy BVS, Raju BDP. Green synthesis
of silver nanoparticles using Ocimmum
leaf extract and their characterization, Dig
J Nanomater Bios. 2011; 6(1): 181 –186.
24. Prasad KS, Pathak D, Patel A, Dalwadi P,
Prasad R, Patel P, Selvaraj K. Biogenic
synthesis of silver nanoparticles using
Nicotiana tobaccum leaf extract and study
of their antibacterial effect. Afr J
Biotechnol. 2011; 10 (41): 8122- 8130.
25. Prasad TNVKV, Elumalai EK.
Biofabrication of Ag nanoparticles using
Moringa oleifera leaf extract and their
antimicrobial activity. Asian Pac J Trop
Biomed. 2011; 439- 442.
26. Kaviya S, Santhanalakshmi J,
Viswanathan B. Green synthesis of silver
nanoparticles using Polyalthia longifolia
leaf extract along with D-Sorbitol: Study
of antibacterial activity. J Nanotechnol.
2011; 1- 5.
27. Korbekandi H, Asghari G, Jalyer SS,
Jalyer MS, Bandegani M. Nanosilver
particle production using Juglans regia
L.(Walnut) leaf extract. Jundishapur J Nat
Pharm Prod. 2013; 8(1): 1-7.
28. Ankanna S, Prasad TNVKV, Elumalai
EK, Savithramma N. Production of
biogenic silver nanoparticles using
Boswellia ovalifoliolata stem bark. Dig J
Nanomater Bios. 2010; 5(2): 369- 372.