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1. Detection of proteins by
solubility test.
Presented To: Mam Dr. Qandeel
Presenter: Waqas Siddiqe
Roll no: 059

2. Remember…
Safety First!
(Here are some instructions you have to follow before doing experiment. )

3. Safety Measures for the lab
Dress for the
Lab.
Do not pipette
by mouth.
Don't Eat or
Drink in the
Laboratory.
Know the
Location of
Safety
Equipment
Don't Taste or
Sniff
Chemicals.
Dispose of Lab
Waste
Properly

4. STEPS TO BE DISCUSS
Major Steps are given below.
1st Principle
2nd Apparatus
3rd Chemical reagents

5. STEPS TO BE DISCUSS
4th Procedure
5th Observation
6th Result

6. Principle:
This test is used to differentiate keratin from the rest of the
proteins.
Principle:
The solubility test is based on the ability of proteins to
dissolve in water other than keratin. Keratin is a large fibrous
protein having numerous disulfide bonds. These disulfide
bonds make it insoluble in water while the rest of the proteins
are highly soluble due to hydrophilic amino acids present in
them.

7. Apparatus:
Pipette, Test
Tube holder
Sucker, Test
tube stand
Test tubes,
Dropper

8. Chemicals:
Unknown
Sample
Distilled
Water

9. Procedure:
Take 10 ml distilled water in
a test tube
Add the given powder into
the water
 Shake the test tube

10. Observation:
• If the given powder is
insoluble in water, it
indicates the insoluble
protein is keratin.

11. Successful Testing
If the sample is insoluble in
distilled water then it confirms
the presence of keratin.

12. Points to remember:
• Most of the proteins are soluble in water due to hydrophilic
amino acids present on their outer surface.
• Fibrous proteins are usually insoluble in water. Keratin is the
most important fibrous protein present in hair, nails, and other
similar structure.
Precautions:
• Read the entire procedure carefully.
• Use clean and dry test tubes for the experiment.
• Protect your hands, eyes and skin from acids, other corrosive
substances.

13. Applications of protein solubility test
Uses of Solubility Tests of Proteins:
• The solubility tests of proteins help to understand the
pattern of solubility of proteins which then helps in the
detection and differentiation of such proteins.
• Solubility testing of proteins enables the selection of
proteins for their use in liquid foods and beverages.
• The solubility of proteins also provides information about
the amino acid composition and molecular weight.

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