Quality control procedures must be followed for specimen collection, preparation of reagents, laboratory techniques, and examination of results to ensure accurate diagnosis of parasitic infections. Specimens like stool and blood must be properly collected and handled to prevent deterioration. Reagents have expiration dates and must be prepared and stored correctly. Laboratory techniques must be performed carefully according to standardized procedures. Examination of final samples, like blood films, requires a quality sample and careful evaluation to avoid errors. Proper specimen collection times are important for certain parasites like malaria and filaria detected in blood.
The document provides guidelines for the proper collection, transport, and processing of specimens submitted to clinical laboratories. It emphasizes that specimen quality is crucial for accurate laboratory results and patient care. Specimens must be collected, labeled, transported, and processed according to specific guidelines to preserve the integrity of the samples and ensure safety. Acceptability criteria are outlined, and specimens that do not meet the criteria may be rejected or require re-collection to avoid misleading results.
Fecal examination is commonly used to diagnose parasitic infections in animals. The process involves collecting a fresh fecal sample, preparing it using flotation or centrifugation with a flotation medium, and examining it under a microscope. Centrifugation speeds up the process by forcing heavier materials to the bottom and lighter parasite eggs to the top for easier identification. Examination of properly collected and prepared fecal samples can reveal evidence of parasitic infections and provide a diagnosis.
This document provides guidance on specimen management for nursing students. It discusses the importance of proper specimen collection, handling, transportation and storage to ensure quality laboratory test results. Key points include:
- Specimen management is important to reduce laboratory errors and involves proper instructions, collection, handling, transportation and storage.
- Universal precautions should be followed when collecting all specimens to prevent exposure to biohazards. Samples must be properly labeled and packaged for transport.
- The type of specimen and collection method depends on the infection or test being performed. Examples provided include blood, urine, stool and sputum collection procedures.
- Transport media is used to maintain specimen viability during transit to the laboratory and varies based on specimen type. Strict
The document provides an overview of parasitology and techniques for diagnosing parasitic infections through stool examination. It discusses factors required for reliable diagnosis such as travel history and appropriate specimen collection. It also describes various stool examination techniques including direct wet mounts, concentration methods, and permanent staining. Flotation and sedimentation are outlined as concentration procedures. Considerations for stool collection kits and preservatives are also summarized.
Chapter XIV Quality assurance in Bacteriology.pptStephenNjoroge22
This document discusses the importance of quality assurance in microbiology laboratories. It emphasizes that test reports must be reliable, standardized, and provide needed information in a clear format. Quality assurance is also necessary to minimize waste and ensure appropriate use of investigations. The document outlines steps for providing reliable, affordable microbiological services, including identifying priority pathogens, techniques and reporting systems to use, quality assurance procedures, training and equipment needs. It provides examples of internal quality control procedures for gram stains, AFB stains, urine and blood cultures. The document concludes with safe working practices for handling infectious materials in the laboratory.
This document provides information about parasitology practice and laboratory diagnosis of parasites. It discusses different types of specimens that can be examined for parasites, including stool, blood, skin, sputum, vaginal discharge, and tissue biopsies. For stool samples, it describes proper collection and preservation methods. It also lists the common parasites that may be observed in different specimen types. Methods for microscopic examination of stool samples include direct smears and concentration techniques like floatation, sedimentation, and others.
This document describes the procedure for a stool wet mount preparation. A saline solution is used as an isotonic media to examine pathogens found in stool samples under a microscope. The procedure involves applying saline and stool sample to a slide, mixing with an applicator stick, examining under a microscope at various magnifications, and reporting any observed ova and larvae. Stool wet mounts are a basic diagnostic technique valuable for identifying parasites by their morphology and are useful for laboratories without advanced equipment. Potential errors include improper preparation, microscope adjustment, sample handling, or disposal of materials.
The document outlines principles for proper collection and submission of specimens to the microbiology lab in order to obtain accurate test results. It discusses 9 key principles: 1) collecting specimens with minimal contamination close to site of infection, 2) collecting at optimal times, 3) obtaining sufficient quantity, 4) using appropriate collection devices and containers, 5) collecting before antibiotics, 6) proper labeling for transport, 7) minimizing transport time, 8) following special handling instructions, and 9) collecting the proper specimen for the ordered test. Specific guidelines are provided for various specimen types like blood, urine, sputum etc. to ensure specimens are of high quality for microbiological analysis.
The document provides guidelines for the proper collection, transport, and processing of specimens submitted to clinical laboratories. It emphasizes that specimen quality is crucial for accurate laboratory results and patient care. Specimens must be collected, labeled, transported, and processed according to specific guidelines to preserve the integrity of the samples and ensure safety. Acceptability criteria are outlined, and specimens that do not meet the criteria may be rejected or require re-collection to avoid misleading results.
Fecal examination is commonly used to diagnose parasitic infections in animals. The process involves collecting a fresh fecal sample, preparing it using flotation or centrifugation with a flotation medium, and examining it under a microscope. Centrifugation speeds up the process by forcing heavier materials to the bottom and lighter parasite eggs to the top for easier identification. Examination of properly collected and prepared fecal samples can reveal evidence of parasitic infections and provide a diagnosis.
This document provides guidance on specimen management for nursing students. It discusses the importance of proper specimen collection, handling, transportation and storage to ensure quality laboratory test results. Key points include:
- Specimen management is important to reduce laboratory errors and involves proper instructions, collection, handling, transportation and storage.
- Universal precautions should be followed when collecting all specimens to prevent exposure to biohazards. Samples must be properly labeled and packaged for transport.
- The type of specimen and collection method depends on the infection or test being performed. Examples provided include blood, urine, stool and sputum collection procedures.
- Transport media is used to maintain specimen viability during transit to the laboratory and varies based on specimen type. Strict
The document provides an overview of parasitology and techniques for diagnosing parasitic infections through stool examination. It discusses factors required for reliable diagnosis such as travel history and appropriate specimen collection. It also describes various stool examination techniques including direct wet mounts, concentration methods, and permanent staining. Flotation and sedimentation are outlined as concentration procedures. Considerations for stool collection kits and preservatives are also summarized.
Chapter XIV Quality assurance in Bacteriology.pptStephenNjoroge22
This document discusses the importance of quality assurance in microbiology laboratories. It emphasizes that test reports must be reliable, standardized, and provide needed information in a clear format. Quality assurance is also necessary to minimize waste and ensure appropriate use of investigations. The document outlines steps for providing reliable, affordable microbiological services, including identifying priority pathogens, techniques and reporting systems to use, quality assurance procedures, training and equipment needs. It provides examples of internal quality control procedures for gram stains, AFB stains, urine and blood cultures. The document concludes with safe working practices for handling infectious materials in the laboratory.
This document provides information about parasitology practice and laboratory diagnosis of parasites. It discusses different types of specimens that can be examined for parasites, including stool, blood, skin, sputum, vaginal discharge, and tissue biopsies. For stool samples, it describes proper collection and preservation methods. It also lists the common parasites that may be observed in different specimen types. Methods for microscopic examination of stool samples include direct smears and concentration techniques like floatation, sedimentation, and others.
This document describes the procedure for a stool wet mount preparation. A saline solution is used as an isotonic media to examine pathogens found in stool samples under a microscope. The procedure involves applying saline and stool sample to a slide, mixing with an applicator stick, examining under a microscope at various magnifications, and reporting any observed ova and larvae. Stool wet mounts are a basic diagnostic technique valuable for identifying parasites by their morphology and are useful for laboratories without advanced equipment. Potential errors include improper preparation, microscope adjustment, sample handling, or disposal of materials.
The document outlines principles for proper collection and submission of specimens to the microbiology lab in order to obtain accurate test results. It discusses 9 key principles: 1) collecting specimens with minimal contamination close to site of infection, 2) collecting at optimal times, 3) obtaining sufficient quantity, 4) using appropriate collection devices and containers, 5) collecting before antibiotics, 6) proper labeling for transport, 7) minimizing transport time, 8) following special handling instructions, and 9) collecting the proper specimen for the ordered test. Specific guidelines are provided for various specimen types like blood, urine, sputum etc. to ensure specimens are of high quality for microbiological analysis.
safety and biosecurity (lab5).Laboratory techniques - How to Avoid Injuries ...Raghda alomari
This document provides guidelines for safely handling specimens in the laboratory to avoid injuries and laboratory-acquired infections. Specimen containers should be strong, leakproof, and correctly labeled. Secondary containers that are autoclavable should be used to transport specimens within the lab and prevent accidental spills. Areas for receiving specimens should be designated, and personnel should be trained to use standard precautions like gloves, gowns, and safety equipment when unpacking packages in case of broken containers. Eating, drinking, applying cosmetics and touching the face are prohibited in the lab. Only trained staff should separate serum while wearing proper protective equipment to prevent splashes or aerosols.
This document provides guidance on collecting, handling, and transporting specimens for laboratory diagnosis of animal diseases. It discusses the purpose of collecting samples, basics of sample collection including safety procedures and equipment needed, specific samples to collect for various diseases, handling and transport of samples, writing laboratory reports, sample storage and archiving. The overall aim is to provide veterinarians and field staff with information to properly collect diagnostic samples that can be safely transported to the lab for accurate disease diagnosis and surveillance.
This document discusses urinalysis and urine testing procedures. It describes the materials needed for dipstick testing, including test strips, gloves, and a urine sample. The test procedure and sources of error are outlined. Different types of urine samples are described, such as random, early morning, and 24-hour samples. Proper collection and preservation of urine specimens is important to avoid deterioration. Urine can be tested physically, chemically, and microscopically. Physical examination evaluates color, turbidity, and odor. Chemical testing uses reagent strips to detect substances. Microscopic examination finds elements like crystals, casts, cells, and microbes that are not visible to the naked eye.
This document provides an overview of medical parasitology and laboratory diagnosis of parasitic infections. It discusses key topics such as the definition of parasitology and medical parasitology. Various specimen types that can be used for diagnosis are described, including stool, blood, urine and others. Main diagnostic methods covered include microscopy, concentration techniques, staining of smears, and newer molecular methods. Specific procedures for examining common specimen types like stool, blood, and urine are outlined.
The document provides guidelines for collecting and examining stool samples to diagnose intestinal parasites microscopically. It states that samples should be 10g of fresh stool collected in a clean container without contaminants, and properly labeled. Examination requires systematic microscopic review of mounts made with preservation solutions like formalin, focusing up and down to avoid missing parasites. Non-parasite artifacts like yeast, pus or plant material could be mistaken for parasites. Floatation techniques using saturated salt or sugar solutions can identify parasite eggs, larvae or cysts of different specific gravities for diagnosis. Treatment thresholds vary by parasite and host species.
This document provides guidelines for sample collection, storage, transportation and labelling for laboratory testing. Key points include:
- Samples should be properly labelled with animal ID, date, owner details and tests requested.
- Transportation conditions like temperature and containers used must maintain sample integrity.
- Storage conditions vary by sample type but generally include refrigeration, freezing or using transport media.
- Guidelines are provided for collecting different sample types from live/dead animals including blood, serum, tissues and ensuring adequate documentation.
Collection, transport & storage of clinical specimensDolatsinh Zala
The document provides guidelines for safely collecting, transporting, and storing clinical specimens. It recommends using personal protective equipment like gloves and lab coats during collection. Specimens should be placed in leakproof containers and transported quickly in dedicated transport bags or autoclaved before disposal. For transport over long distances, specimens must be packaged in a triple container system with absorbent material to contain any leaks. Proper storage conditions are also outlined depending on the specific test or specimen type.
This document provides guidance on collecting various specimen types, including blood, urine, deep specimens, feces, sputum, and genital specimens. It emphasizes the importance of collecting specimens correctly and transporting them to the laboratory promptly to ensure quality results. Key steps include using the proper collection container, obtaining informed consent, maintaining aseptic technique, and documenting and labeling the specimens accurately.
Collection and Handling of Specimens for Laboratory DiagnosisPerez Eric
This document provides guidelines for collecting and handling specimens for laboratory diagnosis of animal diseases. It discusses the importance of collecting sufficient samples before treatment for various diagnostic tests. Guidelines are provided for collecting samples from live animals and during necropsy, including types of samples, containers, labeling, and storage/transport. Proper personal protective equipment, supplies, and documentation are emphasized to ensure sample integrity and lab results.
Air Monitoring by Passive Sampling (lab).pptxkhadeejaahmad4
Pharmaceutical microbiology concerns the use of microorganisms in pharmaceutical development and contamination control. Living microorganisms are found everywhere, including suspended in air, so it is essential to monitor air for microbial and fungal contaminants which could settle on exposed surfaces. Passive air sampling involves placing sterile agar plates in areas for 15 minutes to collect any airborne microorganisms, then sealing and refrigerating the plates until shipping to a lab for analysis within 48-72 hours. Lab results indicating more than 15 colony forming units per plate may be considered out of specification.
The document discusses sample collection and handling for bacterial culture and antibiotic sensitivity testing in veterinary clinical microbiology. It provides guidance on collecting various sample types like exudates, tissues, blood, urine and swabs from different sites while avoiding contamination. The importance of clinical history and proper transport and storage of samples is emphasized. Different methods for antimicrobial susceptibility testing including disc diffusion, dilution and molecular methods are overviewed. Common sets of drugs used in routine susceptibility testing are also listed.
Specimen collection and proper waste management are critical in microbiology. Specimens must be obtained and transported carefully to minimize contamination and ensure viability. Proper containers and labeling are required. Microbiological waste includes sharps, solids and liquids and must be disposed of safely through methods like incineration, autoclaving, or encapsulation using appropriate containers. Laboratories should follow guidelines for collection, transport, and disposal to obtain accurate results and protect health.
The document provides guidelines for collecting and transporting various medical specimens for microbiological laboratory testing. It discusses appropriate collection, labeling, and transport methods for common specimens including blood, urine, sputum, swabs, stool, pus, and cerebrospinal fluid. Proper collection and rapid transport of adequate and correctly labeled samples are essential for successful laboratory investigations and accurate patient diagnosis and treatment.
The document discusses various evaluation tests performed on parenteral products, including sterility testing, clarity testing, leakage testing, pyrogen testing, and assay. Sterility testing involves incubating samples in culture media to check for microbial growth. Clarity testing examines products for visible particles. Leakage testing checks for cracks in ampoules. Pyrogen testing involves injecting products into rabbits to monitor for fever responses. Assay is performed to quantify the active ingredient in the parenteral preparation according to pharmacopeia methods. Proper testing helps ensure parenteral products are free of contaminants and contain the correct amount of active pharmaceutical ingredient.
The presentation summarises important methods and protocols of Clinical Microbiology. It may be useful to learners of Clinical microbiology at the undergraduate label. The presentation describes the procedures for collecting clinical samples, transport, and testing. It also describes the different methods of antimicrobial susceptibility testing and standards.
This document provides guidelines for proper blood collection and handling of clinical specimens. It describes the steps for venipuncture including identifying the patient, selecting a vein, and collecting samples in various tubes according to order of draw. It also discusses collection and preservation of other specimens like sputum, urine, stool and CSF. The aim is to ensure samples are collected, transported and processed correctly for accurate diagnostic testing.
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
How to Build a Module in Odoo 17 Using the Scaffold MethodCeline George
Odoo provides an option for creating a module by using a single line command. By using this command the user can make a whole structure of a module. It is very easy for a beginner to make a module. There is no need to make each file manually. This slide will show how to create a module using the scaffold method.
safety and biosecurity (lab5).Laboratory techniques - How to Avoid Injuries ...Raghda alomari
This document provides guidelines for safely handling specimens in the laboratory to avoid injuries and laboratory-acquired infections. Specimen containers should be strong, leakproof, and correctly labeled. Secondary containers that are autoclavable should be used to transport specimens within the lab and prevent accidental spills. Areas for receiving specimens should be designated, and personnel should be trained to use standard precautions like gloves, gowns, and safety equipment when unpacking packages in case of broken containers. Eating, drinking, applying cosmetics and touching the face are prohibited in the lab. Only trained staff should separate serum while wearing proper protective equipment to prevent splashes or aerosols.
This document provides guidance on collecting, handling, and transporting specimens for laboratory diagnosis of animal diseases. It discusses the purpose of collecting samples, basics of sample collection including safety procedures and equipment needed, specific samples to collect for various diseases, handling and transport of samples, writing laboratory reports, sample storage and archiving. The overall aim is to provide veterinarians and field staff with information to properly collect diagnostic samples that can be safely transported to the lab for accurate disease diagnosis and surveillance.
This document discusses urinalysis and urine testing procedures. It describes the materials needed for dipstick testing, including test strips, gloves, and a urine sample. The test procedure and sources of error are outlined. Different types of urine samples are described, such as random, early morning, and 24-hour samples. Proper collection and preservation of urine specimens is important to avoid deterioration. Urine can be tested physically, chemically, and microscopically. Physical examination evaluates color, turbidity, and odor. Chemical testing uses reagent strips to detect substances. Microscopic examination finds elements like crystals, casts, cells, and microbes that are not visible to the naked eye.
This document provides an overview of medical parasitology and laboratory diagnosis of parasitic infections. It discusses key topics such as the definition of parasitology and medical parasitology. Various specimen types that can be used for diagnosis are described, including stool, blood, urine and others. Main diagnostic methods covered include microscopy, concentration techniques, staining of smears, and newer molecular methods. Specific procedures for examining common specimen types like stool, blood, and urine are outlined.
The document provides guidelines for collecting and examining stool samples to diagnose intestinal parasites microscopically. It states that samples should be 10g of fresh stool collected in a clean container without contaminants, and properly labeled. Examination requires systematic microscopic review of mounts made with preservation solutions like formalin, focusing up and down to avoid missing parasites. Non-parasite artifacts like yeast, pus or plant material could be mistaken for parasites. Floatation techniques using saturated salt or sugar solutions can identify parasite eggs, larvae or cysts of different specific gravities for diagnosis. Treatment thresholds vary by parasite and host species.
This document provides guidelines for sample collection, storage, transportation and labelling for laboratory testing. Key points include:
- Samples should be properly labelled with animal ID, date, owner details and tests requested.
- Transportation conditions like temperature and containers used must maintain sample integrity.
- Storage conditions vary by sample type but generally include refrigeration, freezing or using transport media.
- Guidelines are provided for collecting different sample types from live/dead animals including blood, serum, tissues and ensuring adequate documentation.
Collection, transport & storage of clinical specimensDolatsinh Zala
The document provides guidelines for safely collecting, transporting, and storing clinical specimens. It recommends using personal protective equipment like gloves and lab coats during collection. Specimens should be placed in leakproof containers and transported quickly in dedicated transport bags or autoclaved before disposal. For transport over long distances, specimens must be packaged in a triple container system with absorbent material to contain any leaks. Proper storage conditions are also outlined depending on the specific test or specimen type.
This document provides guidance on collecting various specimen types, including blood, urine, deep specimens, feces, sputum, and genital specimens. It emphasizes the importance of collecting specimens correctly and transporting them to the laboratory promptly to ensure quality results. Key steps include using the proper collection container, obtaining informed consent, maintaining aseptic technique, and documenting and labeling the specimens accurately.
Collection and Handling of Specimens for Laboratory DiagnosisPerez Eric
This document provides guidelines for collecting and handling specimens for laboratory diagnosis of animal diseases. It discusses the importance of collecting sufficient samples before treatment for various diagnostic tests. Guidelines are provided for collecting samples from live animals and during necropsy, including types of samples, containers, labeling, and storage/transport. Proper personal protective equipment, supplies, and documentation are emphasized to ensure sample integrity and lab results.
Air Monitoring by Passive Sampling (lab).pptxkhadeejaahmad4
Pharmaceutical microbiology concerns the use of microorganisms in pharmaceutical development and contamination control. Living microorganisms are found everywhere, including suspended in air, so it is essential to monitor air for microbial and fungal contaminants which could settle on exposed surfaces. Passive air sampling involves placing sterile agar plates in areas for 15 minutes to collect any airborne microorganisms, then sealing and refrigerating the plates until shipping to a lab for analysis within 48-72 hours. Lab results indicating more than 15 colony forming units per plate may be considered out of specification.
The document discusses sample collection and handling for bacterial culture and antibiotic sensitivity testing in veterinary clinical microbiology. It provides guidance on collecting various sample types like exudates, tissues, blood, urine and swabs from different sites while avoiding contamination. The importance of clinical history and proper transport and storage of samples is emphasized. Different methods for antimicrobial susceptibility testing including disc diffusion, dilution and molecular methods are overviewed. Common sets of drugs used in routine susceptibility testing are also listed.
Specimen collection and proper waste management are critical in microbiology. Specimens must be obtained and transported carefully to minimize contamination and ensure viability. Proper containers and labeling are required. Microbiological waste includes sharps, solids and liquids and must be disposed of safely through methods like incineration, autoclaving, or encapsulation using appropriate containers. Laboratories should follow guidelines for collection, transport, and disposal to obtain accurate results and protect health.
The document provides guidelines for collecting and transporting various medical specimens for microbiological laboratory testing. It discusses appropriate collection, labeling, and transport methods for common specimens including blood, urine, sputum, swabs, stool, pus, and cerebrospinal fluid. Proper collection and rapid transport of adequate and correctly labeled samples are essential for successful laboratory investigations and accurate patient diagnosis and treatment.
The document discusses various evaluation tests performed on parenteral products, including sterility testing, clarity testing, leakage testing, pyrogen testing, and assay. Sterility testing involves incubating samples in culture media to check for microbial growth. Clarity testing examines products for visible particles. Leakage testing checks for cracks in ampoules. Pyrogen testing involves injecting products into rabbits to monitor for fever responses. Assay is performed to quantify the active ingredient in the parenteral preparation according to pharmacopeia methods. Proper testing helps ensure parenteral products are free of contaminants and contain the correct amount of active pharmaceutical ingredient.
The presentation summarises important methods and protocols of Clinical Microbiology. It may be useful to learners of Clinical microbiology at the undergraduate label. The presentation describes the procedures for collecting clinical samples, transport, and testing. It also describes the different methods of antimicrobial susceptibility testing and standards.
This document provides guidelines for proper blood collection and handling of clinical specimens. It describes the steps for venipuncture including identifying the patient, selecting a vein, and collecting samples in various tubes according to order of draw. It also discusses collection and preservation of other specimens like sputum, urine, stool and CSF. The aim is to ensure samples are collected, transported and processed correctly for accurate diagnostic testing.
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
How to Build a Module in Odoo 17 Using the Scaffold MethodCeline George
Odoo provides an option for creating a module by using a single line command. By using this command the user can make a whole structure of a module. It is very easy for a beginner to make a module. There is no need to make each file manually. This slide will show how to create a module using the scaffold method.
A workshop hosted by the South African Journal of Science aimed at postgraduate students and early career researchers with little or no experience in writing and publishing journal articles.
ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...PECB
Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
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How to Manage Your Lost Opportunities in Odoo 17 CRMCeline George
Odoo 17 CRM allows us to track why we lose sales opportunities with "Lost Reasons." This helps analyze our sales process and identify areas for improvement. Here's how to configure lost reasons in Odoo 17 CRM
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How to Make a Field Mandatory in Odoo 17Celine George
In Odoo, making a field required can be done through both Python code and XML views. When you set the required attribute to True in Python code, it makes the field required across all views where it's used. Conversely, when you set the required attribute in XML views, it makes the field required only in the context of that particular view.
The simplified electron and muon model, Oscillating Spacetime: The Foundation...RitikBhardwaj56
Discover the Simplified Electron and Muon Model: A New Wave-Based Approach to Understanding Particles delves into a groundbreaking theory that presents electrons and muons as rotating soliton waves within oscillating spacetime. Geared towards students, researchers, and science buffs, this book breaks down complex ideas into simple explanations. It covers topics such as electron waves, temporal dynamics, and the implications of this model on particle physics. With clear illustrations and easy-to-follow explanations, readers will gain a new outlook on the universe's fundamental nature.
3. Learning Objectives
Upon completion of this chapter the student will be able to:
Describe quality control methods in
Stool specimen collection, processing, examination, reporting and disposal
Blood specimen collection, processing, examination, reporting and disposal
Apply the knowledge of quality assurance in the routine
Parasitology laboratory
4. Introduction
To ensure accurate and reliable results, quality control
must be applied to laboratory procedures for diagnosing
parasitic infections
Controls must apply to collection of specimens,
preparation of reagents, performance of the techniques
and examination of the final preparation
5. Quality control for fecal examination
Collection of specimens:
If fecal specimens are not properly collected and taken care
of before examination, they will be of little or no value for
accurate diagnosis
This is especially true of protozoa
Amebic trophozoites will begin to degenerate 1- 2 hours after passage and
alterations in appearance may result in erroneous identification
6. Specimen collection…
Flagellate trophozoites may also undergo changes that would make
differentiation difficult
Cysts will deteriorate if fecal specimens are left standing for many hours or
overnight, especially if the temperature is high
Helminth eggs and larvae are less affected by the age of the specimen than
are protozoa
7. collection of …
Nevertheless, changes may occur that would affect
identification
Hookworm eggs, for example, may become embryonated and larvae may
hatch from the eggs
Even Ascaris eggs may develop to multicellular stages
In addition, larvae may degenerate in old stools making it impossible to
identify the species
8. Collection of …
To ensure that good specimens are provided for
examination, pay attention to the following points:
Use clean, dry containers for collecting feces
Dirt will interfere with examinations and may
introduce free – living organisms from the soil that
would cause problems in identifying the species
Urine and water will destroy trophozoites, if present
9. Collection of …
Have the specimen brought to the laboratory as soon as it is passed to
prevent deterioration of protozoa and alterations in the morphology of
protozoa and helminths.
Note the patient’s name and the date and time of
passage on the specimen
Accept only freshly passed specimens for examination.
Do not attempt to examine old specimens or those
contaminated with dirt, water, or urine.
10. Collection of …
Instead, ask the patient to pass another specimen
If specimens cannot be examined as soon as they arrive, put them in a
refrigerator (4 – 5O C) or in the coolest, shadiest area in the laboratory. Do
not leave them in the sun
Examine diarrheal specimens and those containing blood and mucus
immediately up on their receipt in the laboratory.
11. Collection of …
Some reagents will last indefinitely if kept properly
stoppered and out of direct sunlight.
Examples are formalin solutions, isotonic saline, fixatives, and alcohol
solutions (unless evaporation occurs)
Other reagents may last for only a short time and are
ineffective if too old
The “life” of each solution is indicated in the direction for
preparing it
12. Collection of …
1. Label all reagents with the date of preparation. Keep
records for each solution. Review these every week and
discard outdated solutions
2. Many of the solutions used in the method for trichrome
stain need to be changed at regular intervals. The intervals
are stated in the directions for the technique and must be
observed. Failure to do so will result in poor preparation
that are of no value for diagnosis
13. Performance of techniques
No procedure used for examining fecal specimens is 100%
effective – that is the procedures will not always recover all
the species present and, if a particular species is present in
only very low numbers, they may fail to demonstrate them
when used on a single specimen. Because the techniques
are not perfect, you should perform them as carefully as
possible for optimum results. Also, be sure to use
techniques that are appropriate for the material you are
examining.
14. Direct wet mounts
1. Be sure the density of the mount is correct. You should be
able to read small print through it (but not too clearly). If
it is too thick or too thin, observation of the elements in
the mount may be difficult
2. Be sure to prepare fresh iodine solutions every 10 – 14
days. Old iodine will not stain cysts properly. The iodine
must not be too strong or the fecal material may clump
and trap organisms in the clumps so that they cannot be
seen. If the iodine is too weak, it will not stain cysts
properly.
15. Concentration procedure
1. Select a fully representative sample of the stool for
concentration
2. Prepare well mixed suspensions of feces and water or saline
3. Use the appropriate quantities of materials
4. Use the correct centrifuge speed and time
5. Prepare and examine mounts carefully as described for
direct wet mounts
16. Concentration procedure
6. Do not discard the tube containing the concentrated
material until you have completed your examination. You
may need to make another mount
17. Staining procedure
1. Select soft portions of the stool, and portions of mucus, if
present, for making smears for staining
2. Be sure that solutions are in good condition. Change them
as described in the procedures for staining. Keep the bottles
of stock solutions tightly closed and store away from direct
sunlight
3. Be sure to keep dishes covered. Keep the covers on except
when putting slides into, or taking them out of the dish.
18. Staining procedure…
If the staining dishes are left uncovered, the solutions may
evaporate or debris may get into them, and adhere to the
preparation.
4. Use the correct quantity of mounting medium. Too much
medium may result in a thick layer through which it will be
difficult to focus, and you will not be able to see the smear
clearly. Too little medium will leave gaps under the
coverslip.
19. Staining procedure…
If the smear was prepared from fresh, unpreserved feces,
areas where there are gaps may become unsatisfactory for
diagnosis. Gaps in the medium also make the mount
difficult to examine
5. Always examine stained smears with the oil immersion
objective. Use the x10 objective to focus on the smear. If the
smear is not uniform, locate an area for examination where
the smear is not too thin or too thick and the staining is
good.
Then change to the oil immersion lens and examine for
protozoan trophozoites and/or cysts.
20. Stool Sample Collection…
Do not accept stool samples collected in matchboxes,
newspapers or other containers that leak, health hazard!
Ask the patient not to mix urine with the stool, destroys
trophozoites if present.
A fresh specimen is required, tell the patient to bring the
sample within 1 hour of collection.
Label the container clearly with the patient name and
laboratory number.
21. Sample Storage and Transportation
Stool samples should be examined the same day.
Liquid, watery or bloodstained stool should be examined
within one hour after collection.
If you find eggs or cysts that you do not know you can
preserve the stool sample in 10% Formalin till an expert
comes or till the sample can be carried to the reference
laboratory.
22. Sample Storage and
Transportation…
Stool specimens for stool culture can not be preserved in
10% Formalin, they must be sent preserved in a special
culture media. (Stuart or Amies)
Samples stained by modified Ziehl - Neelsen stain can be
stored in a box for future examination by a visiting expert
23. Waste Disposal and Precautions:
Consider all stool samples as highly infectious.
Avoid contact with bare fingers, wear gloves if possible or
handle with care.
Do not reuse stool containers, burn stool sample containers
and wooden applicators.
Soak glass slides in 5 % Phenol solution (e.g. Lysol) at least
over night.
Cover slips break easily and may cause injuries, therefore
soak in a separate container in 5 % Phenol solution (e.g.
Lysol) at least over night.
24. Waste Disposal and Precautions…
Chemical waste disposal
Pour old and used chemicals into the sink and flush with
water if the sink is connected to a soak pit.
Otherwise, pour chemicals directly into the soak pit. Ensure
that the soak pit is not near a natural water source.
Formalin is irritating to the skin and the vapor should not
be inhaled.
25. Quality control for blood examination
Sample collection time:
The number of certain parasites in the blood depends on the time of
collection.
Malaria
⇒ Highest number of parasites is found during fever attacks and before
the start of treatment
Microfilaria
⇒ W. bancrofti and Brugia malayi – take specimen at night between 10
p.m. – 2 a.m.
26. Sample collection time…
Smear for Malaria Parasites
Follow proper collection procedures.
Glass slides must be clean and free from grease.
Thick films and thin films must be prepared properly.
While drying protect blood films from dust, flies and
insects.
Do not dry exposed to direct sun light
When fixing the thin film, be careful not to let methanol
27. Possible sources of error in the blood
film examination/ report
Performance of a blood film examination on a poor quality film achieves
inaccurate results.
A poor quality film caused by:
Poor spreading technique
Poor leukocytes distribution
Too small a working area
Red cell morphology altered by the spreading
process.
28. Possible sources …
Use of the wrong anticoagulant, resulting in altered blood cell size and/or
morphology
Patient sample mix up.
Transcriptional error.
Inadequate mixing of the sample with the anticoagulant, resulting in the
formation of small fibrin strands and a decrease in the platelets count.
Poor stain quality etc
29. Sample collection time…
Smear for Microfilaria
Filaria are seldom found in the early and in the late stage
of the disease.
The proper time of collection is important (10 p.m. to 2
a.m. )
Un sheathed non-pathogenic filaria can be found any
time of the day.
Patients with filaria in the blood show also eosinophilia in
the blood.
30. Summary
Quality control procedures must apply to collection of
specimens, preparation of reagents, performance of the
techniques and examination of the final preparation
If fecal specimens are not properly collected and taken care
of before examination, they will be of little or no value for
accurate diagnosis
Performance of a blood film examination on a poor quality film achieves
inaccurate results.
Filaria are seldom found in the early and in the late stage of
the disease. Hence, the proper time of collection is important
31. References
Basics of Quality Assurance. WHO series 2, 1992
Arneson, W and J Brickell: Clinical Chemistry: A Laboratory
Perspective first edition, FA Davis, 2007
Becton Dickinson FACSCount System User’s Guide (For Use
with the BD FACS Count™ CD4/CD3 Reagent Kit)
WHO. Basic Laboratory Methods in Medical Parasitology,
1991, Geneva.