fungus resistant plant via tissue culture

2.  “Plant tissue culture is the collection of tecniques
use to maintain or grow plant cell tissue or organ
under sterile condition on a nutrient culture
medium”

3.  SAR(systemic acquired resistance in plants)
 Over production of salicylic acid
 By the production of Anti-fungal protein

4.  After infection of a plant by a pathogenic fungus
the inactive storage compound salicylic acid 2-o-
beta-glucoside is converted to salicylic or induces
the NPRI gene, whose protein product act as “
master” regulatory protein to turn on the
expression of PR protein,
 Which have enzyme activities directed against
various pathogenic organism.

6.  Another approach to engineering plant with broad
spectrum diseases resistant involves
overproducing salicylic acid.

7.  Firstly plant transforming with bacterial genes that
encode the enzyme ICS (isochorismate synthase)
and IPL(isochorismate pyruvate lyase) which
catalyze salicylate synthase.
 Salicylate is synthesized from chorismate in large
amount in chloroplast.
 Which is also intermediate in the biosynthesis of
the amino acid tryptophan.

9.  Pathogenic fungi belong to the genus fusarium are
the causative agents for the plants.
 Their for strategy that target various fusarium
spp.microbial peptide and chitinase that inhibit the
growth of fusarium .
 For this two different antimicrobial peptide from
radish(Raphanus sativus)and one from mold
(Asperigillus giganteus) and chitinase(from wheat) to a
single chain.

10.  Fv antibody that binds to a fusarium cell wall
protein.
 Fv antibody constructed from chickens.
 When fusarium on attack on A.thaliana, plant this
single-chain Antibody inhibit the growth.
 All three of the antibody-peptide fusion conferred
a high level of resistance to demage by the
pathogen

11.  Phytopathogenic fungus badly effect the crop
yield.
 Fungal rice blast disease that effect the rice plants
 Pathogenic fungi belong to genus fusarium is the
most devastating disease in the world.

12.  The major way to protect plants and crop from
fungal infection through chemical antigen.
 By producing pathogenic plants.
 By the formation of high level of chitinase in
transgenic plants.
 By increasing the level of salicylate in plants.
 By preparing sterilized envirment for the plants.