shoot regeneration from callus

2.  A collection of techniques used to maintain or
grow plant cells, tissues or organs
 Under sterile conditions on a
nutrient culture medium
 Used to produce clones of a plant in a method
known as micropropagation.

4.  a growing mass
 unorganized plant parenchyma cells.
 callus formation is induced from plant tissue
samples (explants)

6.  The process of growing an entire plant from a
single cell or group of cells.
 the physiological renewal, repair, or
replacement of tissue in plants.

7.  conical containers
 Forceps
 Magnetic stirrer
 Agar
 sucrose
 plant growth regulators
 aluminum foil

8.  Scissors
 Knife
 Beakers
 sodium hypochlorite solution
 70% alcohol

9.  Laminar Flow Cabinet
 Autoclave
 Magnetic stirrer
 Oven
 Sprit lamp

10.  Choose an explant
 Wash it with tap water for 10 minutes
 Place it in 1% sodium hypochlorite solution
 Wash it with distilled water
 All the equipments are sterilized by placing in
autoclave

11.  Then the explant with the nutrient media is
place din the laminar flow air cabinet
 After callus formation from explants the
formed calli are transferred into conical
containers
 Levels were used as cytokinin and auxin
sources