The Microbiology Laboratory is located in the Department of Biology, Science and Technology Faculty of Universitas Airlangga

1. UNIVERSITAS AIRLANGGA
SURABAYA - INDONESIA
2018

2. The Microbiology Laboratory is located in the Department
of Biology, Science and Technology Faculty of
Universitas Airlangga

3. The activities related to microbial utilization
between September 2017 – present
Universitas Airlangga
2018

4. The activities of Microbiology Lab
and Biochemie Lab
• Reasearch all about microbiology and biochemie
for student of bachelor, master, and doctoral
• Public services
• Community service

5. • Bioremediation and biodegradation
• Biodiversity of Microbes
• Biofertilizer
• Bioconversion
• Probiotics
• Natural product
• Sensitivity of antimicrobial agent
• Detection of encoding gen for
biodegradation and biosurfactant
production in microbes
Researches

6. RESEARCHES TOPICS

7. Bioremediation and Biodegradation
Biodegradation of oil sludge using various consentrations of bacterial
consortium and incubation times
Potency of Azolla pinnata compost as bulking agent in bioremediation
Efects of consotiums of bacteria, bulking agent, and various incubation time on
Oil Sludge biodegradation
Oil sludge biodegradation using consortium of bacteria with various nutrients
and incubation times
Effect of various concentrations of Micrococcus sp. LII61 and incubation times
on biodegradation of oil sludge
Bioremediation of heavy metal Cr on oil sludge by Pseudomonas aeruginosa
The effect of adding molasses to Anaerobic- aerobic continue reactor in the
process of removing Chemical Oxygen Demand and Total Ammonia Nitrogen
on Tempe wastewater
Decrease in biologycal oxygen demand (BOD) and chemical oxygen demand
(COD) on liquid waste chicken slaughterhouse using microbial consortium
Biodegradation of oil sludge with various concentrations of pollutantand
incubation times on bioslurry reactor

8. Bioremediation and Biodegradation
The effect of dilution and addition of urea in the wastewater know on cultivation
of Chlorella vulgaris to decrease chemical oxygen demand (COD) and
ammonium
Application of biosurfactant produced by Pseudomonas putida on soil washing
techniques contaminated with oil
Polyethylene biodegradation using various cocentrations of consortium bacteria
(Staphylococcus and Bacillus) and incubation time
Biodegradation of Naphthalene and Phenanthrene by Arthrobacter sp.
Effect of various concentrations and incubation times of bacterial consortium
Bacillus and Alcaligenes on Polyethylene biodegradation
Biodiversity
Isolation and Screening of Proteolytic Bacteria
Isolation and Screening of Lipolytic bacteria
Isolation, Characterization, and Identification of Burkholderia glumae from
Oryza sativa L.
Isolation, Characterization, and activity test of potential indigenous bacteria to
degrade pharmacy industry wastes.

9. Biofertilizer
Effect of variations doses of biofertilizer and compost on Zea mays
Effect of Various doses of biofertilizer and compost on Arachis hyogaea L.
Effect of various doses of biofertilizers and organic fertilizer on Vigna sinenis L.
Effect of various doses of biofertilizer and compost on Vigna radiata
Application of various doses and frequency of biofertilizer on Solanum
melongena L. var. sriti.
Study of dose variation and intensity of biofertilizer in increasing Solanum
melongena L. productivity
Utilization of biofertilizer with a combination of dose variation and frequency of
administration against Vigna radiata L.
Sensitivity of Antimicrobial Agent
Antimicrobial test dihydro-pyrimidinone derivate compound in selulase acetate
nano-membrane

10. Probiotics
Effect of probiotics on Litopenaeus vannamei growth and productivity.
Effect of various doses of probiotics on feed and cultivation media on growth and
productivity of Osphronemus goramy
Effect of various concentrations of probiotics on Chanos chanos growth.
Comparison of feed and probiotics variations on Broiler weight
Efects of varios formula and doses of probiotics on increasing weight and feed of
Broiler
Natural Products
Potency of indigenous oil sludge Dumai-Riau in Producing Biosurfactant
Screening of indigenous oil sludge bacteria from Dumai Riau in producing
Biosurfactants
Activity test of enzymes of Bacillus sp. (D1) on organic isopropyl alcohol and and
dichloromethane solvent
Detection and activity test of polyethylene degrading enzymes produced by
Alcaligenes sp. PD6
Detection and activity test of polyethylene degrading enzymes produced by
Bacillus sp. PD4
Production of cellulase enzyme by Penicillium sp. H9

11. Detection of encoding gen in microbes
Detection of Biosurfactant Gene in Bacterial Isolate T1
(8) and Characterization of Biosurfactants Produced on Water-miscible
and Waterimmiscible Substrates
Bioconversion
Effect of glucose and incubation time to Acetic acid fermentation by
Acetobacter acetii
Ethanol fermentation using various concentration by Zymomonas mobilis
Ethanol fermentation using various concentrations of Saccharomyces
cereviseae and incubation times
Effect of various concentrations of Acetobacter acetii and incubation times on
acetic acid fermentations
Effect of various cow dung concentrations and fermentation times on Biogas
Production
Effect of coconut water mediu and fermentation times on Acetic acid
concentration produced by Acetobacter acetii

12. Number of Research in 2017
14
4
7
6
5
6
1 1
31.8
9.1
15.9
13.6
11.4
13.6
2.3 2.3
0
5
10
15
20
25
30
35
Bioremediation
and
biodegradation
Biodiversity of
Microbes
Biofertilizer Bioconversion Probiotics Natural product Sensitivity of
antimicrobial
agent
Detection of
encoding gen in
microbes
Number of research Percentage of research

13. Public services
The microbiology lab service
unit serves microbiological
analysis for the public either
from company or personal
No. Kind of Analysis
1 Analiysis of microbial identification
2 Analysis of nitrogen-fixing microbes
3 Analysis of phosphate solubilizing
microbial
4 Analysis of microbial sensitivity
5 Detection E.coli and coliform
6 Analysis of total microbial (etc.)

14. Community services
No Topic of
community service
Target Time
1 Cultivation of
Pleurotus ostreatus
Desa Jemirahan, Kecamatan
Jabon, Sidoarjo , Indonesi
2017
2 Biotoilet Desa Tenggilis, Surabaya-
Indonesia
2017
3 Biofertilizer Desa Duren, Kabupaten
Probolinggo -Indonesia
2017
4 Biofertilizer Desa Rojoso, Kabupaten
Nganjuk -Indonesia
2017

15. Utilisation of microbial consortium for bifertiliser product

16. Utilisation of microbial consortium for biotoilet product

17. Utilisation of microbial consortium for biodecomposer product

18. Utilisation of microbial consortium for biodegraders agent of
organic waste

19. THANK YOU

20. Biotoilet
Saturday, 5 August 2017
• Desa Tenggilis, Surabaya, Indonesia
• Audience : 19 person
• Method : oral presentation, pretest, postest, and practice

21. Pretest and Postest

22. 2. Saturday, 4 November 2017
• Desa Rojoso, Kabupaten Nganjuk, Indonesia
• Audience : 33 person
• Method : oral presentation, pretest, postest, and practice
Biofertilizer

23. Pretest and Postest
0
10
20
30
40
50
60
70
80
90
0-25 25-50 51-75 76-100
Persentase
Range of score
Pretest
Postest
Pretest and Postest

24. Biofertilizer
1. Sunday, 6 August 2017
• Desa Duren, Kabupaten Probolinggo, Indonesia
• Audience : 30 person
• Method : oral presentation, pretest, postest, and practice

25. Pretest and Postest
0
10
20
30
40
50
60
70
80
90
0-25 25-50 51-75 76-100
Presentase
Range of score
Pretest
postest