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Microbiology Researches at UST
University of Santo Tomas
Pontifical, Royal, and Catholic University of the Philippines
Jaycee Augusto G. Paguirigan, PhD
Department of Biological Sciences
College of Science
Email: jagpaguirigan@gmail.com
Thomas Aquinas Research
Complex
https://ustfungalbiodiversitylab.wordpress.c
om/
Fungal Biodiversity, Ecogenomics and Systematics Group
Isolation of Marine and Terrestrial Strains of Trichoderma
Cultures grown on Trichoderma Selective Medium (TSM) after 3
days of incubation
23 Isolates belonging to 4 morphospecies
Isolation of Aquatic Fungi from Taal Lake
Spread Plate Method
Single Spore Method
Surface Water
Submerged
Wood
Direct Fruiting
Body Mounting
Morphological (for all isolates)
Molecular (for selected isolates)
Extraction Purification Amplification Sequencing
Riddell Slide Culture: spores,
hyphae, conidia, fruiting structures
Nuclear Internal Transcribed Spacer:
ITS1 (forward primer), ITS4 (backward
primer)
ISOLATION OF ENDOLICHENIC FUNGI
IN FOLIOSE and FRUTICOSE LICHENS
ISOLATION OF SOIL FUNGI
FROM GROUND AND CANOPY SOIL
UST
Collectio
n of
Microbial
Strains
Training and
Workshop
Distribution of Microbial
Resources to
Students/Researchers
Database Management
System
CMS Brochures/Flyers
Utilization of Indigenous
Microbial Resources
(Research Projects)
Deposit/Maintenance/
Safekeeping of
Microbial Resources
Student
On-the-Job
Training
Microbiological Services
THE UST COLLECTION OF
MICROBIAL STRAINS
INDIGENOUS
MICROBIAL
COLLECTIONS
Bacteria
(50+)
Molds (50 +)
Yeasts (50+)
Graduate School
Fungal Collection
(c/o Dr. Irineo Dogma)
(650+)
Marine
Oomycetes
(50+Strains)
(11 Molecularly
Identified and 3
New Species)
Actinomycetes
(15 + strains)
Inventory of Microbial Collections
as of March 2019
Database Used for Inventory: iCollect from Biotec, Thailand.
Natural
Products for
Health and
Wellness
Pure and
Applied
Microbiology
Molecular
Diagnostics
and
Therapeutics
Biodiversity,
Ecology,
Systematics &
Taxonomy
REASEACH CENTER FOR THE NATURAL AND APPLIED SCIENCES
RESEARCH LABORATORIES
Use of microbial cultures from CMS
University of Santo Tomas
Pontifical, Royal, and Catholic University of the Philippines
UST CMS Website
University of Santo Tomas
Pontifical, Royal, and Catholic University of the Philippines

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5. ust philippines

  • 1. Microbiology Researches at UST University of Santo Tomas Pontifical, Royal, and Catholic University of the Philippines Jaycee Augusto G. Paguirigan, PhD Department of Biological Sciences College of Science Email: jagpaguirigan@gmail.com
  • 4. Fungal Biodiversity, Ecogenomics and Systematics Group
  • 5. Isolation of Marine and Terrestrial Strains of Trichoderma Cultures grown on Trichoderma Selective Medium (TSM) after 3 days of incubation 23 Isolates belonging to 4 morphospecies
  • 6. Isolation of Aquatic Fungi from Taal Lake Spread Plate Method Single Spore Method Surface Water Submerged Wood Direct Fruiting Body Mounting Morphological (for all isolates) Molecular (for selected isolates) Extraction Purification Amplification Sequencing Riddell Slide Culture: spores, hyphae, conidia, fruiting structures Nuclear Internal Transcribed Spacer: ITS1 (forward primer), ITS4 (backward primer)
  • 7. ISOLATION OF ENDOLICHENIC FUNGI IN FOLIOSE and FRUTICOSE LICHENS
  • 8.
  • 9.
  • 10.
  • 11. ISOLATION OF SOIL FUNGI FROM GROUND AND CANOPY SOIL
  • 12.
  • 13.
  • 14.
  • 15.
  • 16. UST Collectio n of Microbial Strains Training and Workshop Distribution of Microbial Resources to Students/Researchers Database Management System CMS Brochures/Flyers Utilization of Indigenous Microbial Resources (Research Projects) Deposit/Maintenance/ Safekeeping of Microbial Resources Student On-the-Job Training Microbiological Services THE UST COLLECTION OF MICROBIAL STRAINS
  • 17. INDIGENOUS MICROBIAL COLLECTIONS Bacteria (50+) Molds (50 +) Yeasts (50+) Graduate School Fungal Collection (c/o Dr. Irineo Dogma) (650+) Marine Oomycetes (50+Strains) (11 Molecularly Identified and 3 New Species) Actinomycetes (15 + strains) Inventory of Microbial Collections as of March 2019 Database Used for Inventory: iCollect from Biotec, Thailand.
  • 18. Natural Products for Health and Wellness Pure and Applied Microbiology Molecular Diagnostics and Therapeutics Biodiversity, Ecology, Systematics & Taxonomy REASEACH CENTER FOR THE NATURAL AND APPLIED SCIENCES RESEARCH LABORATORIES Use of microbial cultures from CMS University of Santo Tomas Pontifical, Royal, and Catholic University of the Philippines
  • 19. UST CMS Website University of Santo Tomas Pontifical, Royal, and Catholic University of the Philippines