Universitas Negeri Jakarta Culture Collection (UNJCC) was established in 2014 and is located in Jakarta, Indonesia. It contains 106 filamentous fungal isolates and 76 yeast isolates obtained from various studies. The collection aims to explore biodiversity and conduct ecological studies of microbes. It offers identification and enzyme assay services and collaborates internationally. The document provides details on the collection, its research areas involving enzyme production and biocontrol agents, and potential applications of its microbial isolates.

1. UNIVERSITAS NEGERI JAKARTA
Universitas
Negeri Jakarta
Culture Collection
(UNJCC)
Dr. Dalia Sukmawati
Dalia-Sukmawati@unj.ac.id
Presented in ASEAN Network on Microbial
Utilization 25-28 April 2019

2. UNJ is a public university in Jakarta,
Indonesia. Founded in 1964 as the Jakarta
Institute of Teaching and Education (IKIP), it
was later converted into a state university by
the Indonesian government in 1999. UNJ has
vision to be a faculty that has a competitive
advantage in the field of Mathematics and
Natural Sciences (MIPA) and education at the
global level and can produce graduates who
are faithful and devoted.Presented in ASEAN Network on Microbial
Utilization 25-28 April 2019

3. UNIVERSITAS
NEGERI
JAKARTA
UNJCC
➢ Established in 2014
➢ Location :
Universitas Negeri Jakarta Culture Collection
(UNJCC). Biology Department Jl. Rawamangun
Muka Kampus A, Hasyim Ashari Building 9th
Jakarta Timur 17413 Indonesia.
➢ Team:
Dr. Dalia Sukmawati ( Curator Yeast and Fungi)
Dr. Tri Handayani K.(Curator Bacteria)
Dr. Rini Puspitaningrum (Biochemistry Molecular)
Dr. Muktiningsih Nurjayadi (Biochemistry)
Dr. Fera Kurniadewi (Natural Product)
Irma Ratna Kartika, Msc. Tech (Natural Product)
Presented in ASEAN Networkon Microbial Utilization 25-28 April 2019

4. UNIVERSITAS
NEGERI
JAKARTA
Type and Number of
Material
Filamentous Fungi
Total number = 106
Yeast
Total number= 76
Yeast microbes and molds were obtained fromseveral studies conducted at the
MicrobiologyLaboratory, Universitas Negeri JakartaPresented in ASEAN Network on Microbial
Utilization 25-28 April 2019

5. • Exploration of chitinolytic yeast biodiversity from phylloplane of
traditional medicine plant at Indonesia, from chicken feed, and
anthracnose fruit.
• Exploration of chitinolytic yeast and bacteria biodiversity from Kukang
(Nycticebus javanicus), Garut West Java.
• Metagenome of yeast and bacteria from fermented food, Indonesia with
potential producing enzyme amylase, cellulose, chitinase, and phytase.
• Insight into the microbiota of ethnic fermented foods of Bali, Indonesia
using high throughput sequencing.
• Exploration of Ganoderma sp. from Palm tree.
• IndoGenePro Laboratories: Master Diagnostics Salmonella spp.
Detection Kit; Master Diagnostics Shigella spp. Detection Kit; Master
Diagnostics Escherichia coli Detection Kit.
• Biosurfactant of bacteria
Research Area of Lab. Microbiology Genetic Department of
Biology, FMIPA, UNJ.
Exploration of Biodiversity, Microbial Systematics and
Ecological Studies
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6. POTENTIAL APPLICATION
UNIVERSITAS
NEGERI
JAKARTA
Yeast as Potential for Antagonism and Biocontrol Agents,
enzyme production, and Probiotic
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Potential as enzyme production (Amylase and
cellulase

7. POTENTIAL APPLICATION
UNIVERSITAS
NEGERI
JAKARTA
Diversity of Ganoderma sp. from Plam Tree
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Diversity Dark Septae Endofit A. sydowii strain PD5.

8. Clone Palm sp. Resistantto Ganoderma boninese
UNIVERSITAS
NEGERI
JAKARTA
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9. Potential amylase and Cellulase enzyme -producing yeast
from indigenous fermentedbeverages originating
from Bali, Indonesia
UNIVERSITAS
NEGERI
JAKARTA
Diversity isolates of yeast from Fermented
Food with Amylase and Celluase Enzyme
activity (100 isolates)
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10. Metagenome Data Base of Fungi from Indigenous Durio
zibethinus fromWest Borneo, Indonesia
UNIVERSITAS
NEGERI
JAKARTA
Diversity isolates of yeast from durio
sp. Papekan (100 isolates)
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11. Product :
Dr. Muktiningsih Nurjayadi
(Biochemistry Lab).
Master Diagnostic
Detection Kit
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Utilization 25-28 April 2019

12. Master Diagnostic
E.coli Spp
Detection Kit
Product :
Dr. Muktiningsih Nurjayadi
(Biochemistry Lab).
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Utilization 25-28 April 2019

13. Handbook Product
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Utilization 25-28 April 2019

14. UNJCC ACTIVITY
Isolation and Identification
Workshop for Juniorand Senior
High School in Microbiologyfield International training
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Utilization 25-28 April 2019

15. UNIVERSITAS
NEGERI
JAKARTA
Preservation methods
used
➢ L – Drying
➢ Electric
Freezer (-200C)
➢ Sub culturing
➢ Paraffin oil
and glycerol
20%
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Utilization 25-28 April 2019

16. UNIVERSITAS NEGERI JAKARTA
➢ Identification of molecular method 16S rDNA;
D1/D2 region
➢ Morphological characteristic
Data Recording management
Quality Testing
➢ Loogbooks
➢ Logsheets
➢ For our next plan at this moment;
➢ we will implementation Icollect software
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Utilization 25-28 April 2019

17. UNIVERSITAS
NEGERI
JAKARTA
Services for Fungi and
Yeasts
Identification using partial rDNA sequencing: Ribosomal DNA containingsmall (18S or SSU)
and large (26S/28S or LSU) subunitsand the internal transcribed spacer region (rDNA ITS),
has been long used as alternativeto mitochondrial genesfor identificationof Fungi (Seifert
2009; Begerow et al. 2010). UNJCC offers identificationbacteriaand fungi cultures using
sequencing ITS region, D1/D2 rDNA, 16S rDNA, Beta tubulin,Calmodulinandspecific primer
for identification enzymeand protein.Identificationincludes,purification,DNA extraction,
PCR sequencing, and analysisreport with Phylogenetic tree using chromaspro, and PAUP.
Identification of fungi using morphologicalcharacters: identificationof fungal cultures
using morphologicaldescription.
Identification of yeasts using morphologicalcharacters: UNJCC offers identificationprofiles
of maximum growth temperaturewith current guidelines (Kurtzman et al, 2011).
Enzyme assay: Selective Isolationof microbes from tropical habitat(soil sample) which has
high hyrolasesenzyme activities (pectinase, amylase, cellulose, and keratinaseas two case
study of enzymes from different classes). Purificationof microbialcultures using selective
medium. Secondary isolationof targeted enzyme biofactoriesusing agar platemethod.
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18. UNIVERSITAS
NEGERI
JAKARTA
Our Student Team
1. Aditya Risandi
2. Denika Dellanerra
3. Indah Sofiana
4. Anggun Sunari
5. Ulfi Rahma
Efrianti
6. Putri Anisa
7. Noer Azizah
8. Anni Shodrina
9. Fadlin Ary
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19. International Collaboration
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20. UNIVERSITAS
NEGERI
JAKARTA
UNJCC
Thank you for your attention !
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Utilization 25-28 April 2019