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ABOUTBIOFERTILIZERS INDIAN PERSPECTIVE
BIOFERTILIZERS ARE MICROBIAL  INOCULANTS SUPPORTED ON     CARRIERS AND MAKE        AGRICULTURE:    # environmentally susta...
WHAT ARE BIOFERTILIZERS [BF]?• Microbial inoculants that are carrier-based  preparations containing beneficial  microorgan...
Microbial inoculants• Artificially multiplied cultures of certain  soil organisms that can improve soil  fertility and cro...
5
BIOFERTILIZERS: USE IN INDIA# First commercial production of legume  Rhizobium symbiosis was done in 1956# Ninth Plan init...
Factors affecting Performance of                biofertilizers:•   Host plant•   Soil fertility•   Cropping practices•   B...
Rhizobium• Bacteria that grows in root nodules of  legumes [soya bean, etc.]• Rhizobium INOCULANTS:  Use of Rhizobium cult...
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Rhizobium• Rhizobium bacteria in roots of legume  plants fix atmospheric N2 in nodules  formed on the roots of plants.    ...
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Inoculum Application                       12
Inoculum Application                       13
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Non-legume INOCULANTS               Azotobacter# These bacteria enhance the plant  growth and finally yield. A highly effi...
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Azotobacter:• Free living micro-organisms, that grow  in the rhizosphere and fix atmospheric  nitrogen non-symbiotically• ...
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Azospirillum• Azospirillum, [ Azospirillum lipoferum,  biological nitrogen fixing grasses;  enhance biomass-root system]• ...
Azospirillum• Commonly used for preparation of  commercial inoculants on a large  scale, for cereals and grasses as it  pr...
22
Cyanobacteria INOCULANTS::• Biological nitrogen fixing ;  photosynthetic also; Some free living  cyanobacteria like Nostoc...
CyanobacteriaAn important group of micro-organisms, fix atmospheric nitrogen non-symbiotically mostly in rice fields inhet...
Cyanobacteria: PreparationEfficient strains_ used for multiplication on a large scale in field. Production of inoculum in ...
Cyanobacteria: Preparation– # Based on use of starter culture that is  multi-strain inoculum of_ Aulosira,  Tolypothrix, S...
Phosphate SOLUBILIZING            INOCULANTS• Phosphate solubilizing  microorganisms are  Bacteria (Bacillus megaterium) a...
Phosphate Solubilizing bacteria / fungi:Phosphorus_ required for plant growth and water yield. It is also essential for no...
VAM fungi  [Vesicular Arbuscular Mycorrhiza]Intracellular, obligate, fungal endo-  symbiont - glomus etc.- transfers  phos...
A schematic diagram of Vesiculararbuscular mycorrhiza                             30
Mycorrhiza• Colonise 85% of land plants_ offers  50 % saving in chemical P fertilizer_  higher resistance to soil-and-root...
Mycorrhiza Produced by:# M/s Cadila PharamaceuticalLtd., Ahemdabad, and KCPSugar and Industries (Pvt.) Ltd.,Chennai [DBT-T...
Award for Producer of Mycorrhiza• KCP Sugar Industries received  the All India Biotechnology  Association Award for  produ...
Estimated potential Demand for          Biofertilisers by 2000-2001•   Type of Biofertiliser   •   Demand (Tonnes)•   Rhiz...
Biofertilizer Production Support:                           GOI- 1999-2000• National Biofertilizer Development  Centre- es...
Promoting Bio-fertilizers: Current Situation• A limited extent of success till date  (2000).• There has been no accelerate...
Progress of the bio-fertilizer Industry• Based on the data for 1995, 1997  and 1999, the industry witnessed a  steady incr...
Changes in the share by type of BF• moderate success in AZT and by far the best performance by PSB• decline in RHZ indicat...
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40
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Main constraints in spread of        BF as an Industry:1. Inadequate production and supplyof efficient cultures of micro-o...
Marketing: Host, soil & climate specific     BF: Receive user feedback• To cover the vast areas of legumes,  pulses and oi...
Marketing of BF: Logistics• # Prepared bio-fertilizers should reach  farmers prior to sowing.  # If given free of cost, th...
Steps in biofertilizer production     Strain selection,        Mass Culture,   Carrier preparation and         Inoculation...
NATURE OF BF INDUSTRY:•    Indigenous technology•   Scientific aspects: Standardized by    Agricultural Universities and R...
Manufacturing equipments:•   Laboratory equipments,•   Autoclaves,•   Fermenter assembly,•   Boiler,•   Broth dispensers,•...
The size of a Biofertiliser unit:• Capacity of production of various types /  strains of bio-fertilizers per annum.• The p...
Requirements of         BF Projects1 Land2 Layout and buildings3 Plant and Machinery4 Manufacturing process and Source of ...
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SOME OF BIO-FERTILIZERSIN THEMARKET               52
Dr Bhabani Dikshit                     53
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Extension work needed for popularizing BF• Visits to areas of undivided Puri district  comprising of Pipli , Khurda and Na...
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BOOKS ABOUTBIOFERTILIZERS        CONTINUED
Indian Books on Biofertilizers-1• Biofertilizer - Technology, Marketing  and usage, a source book-cum-  Glossary by Dr. M ...
Indian Books on Biofertilizers-2• Biofertilizers in Agriculture and Forestry  by N S Subba Rao, Oxford & IBH, New  Delhi.•...
Indian Books on Biofertilizers -3• Recent Advances in Biofertilizer Technology/  Edited by A.K. Yadav, S. Ray Chaudhuri an...
Handbook of Biofertilizers• Handbook of microbial biofertilizers, M. K. Rai,  Routledge, 2006 - 579 pages,• Sharply focuse...
It examines the role of microbes in growth promotion,bioprotectors, and bioremidiators, and presents protocolsand practica...
… including: the rhizobium-host-arbuscular mycorrhizal"tripartite relationship, mycorrhiza as a disease suppresserand stre...
In this young century, the use of biofertilizers is alreadygrowing rapidly. It has been recognized that theseenvironment-f...
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Notes on biofertilizer

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Biofertilizers are supplement to chemical fertilizers and are beneficial if properly used.

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Notes on biofertilizer

  1. 1. ABOUTBIOFERTILIZERS INDIAN PERSPECTIVE
  2. 2. BIOFERTILIZERS ARE MICROBIAL INOCULANTS SUPPORTED ON CARRIERS AND MAKE AGRICULTURE: # environmentally sustainable, # economically rewarding, # intellectually stimulating.
  3. 3. WHAT ARE BIOFERTILIZERS [BF]?• Microbial inoculants that are carrier-based preparations containing beneficial microorganisms in a viable state.• Intended for seed or soil application.• Designed to improve soil fertility in N and P• Provide growth promoter substances. 3
  4. 4. Microbial inoculants• Artificially multiplied cultures of certain soil organisms that can improve soil fertility and crop productivity.• Rhizobia were discovered in 1895, followed by the Azotobacter and then the blue green algae and a host of other micro-organisms.• Azospirillum and Vesicular- Arbuscular Micorrhizae (VAM) are fairly recent discoveries. 4
  5. 5. 5
  6. 6. BIOFERTILIZERS: USE IN INDIA# First commercial production of legume Rhizobium symbiosis was done in 1956# Ninth Plan initiated National Project on Development and Use of Biofertilizers (NPDB).# To supplement chemical fertilizers that become expensive and deteriorate soil. 6
  7. 7. Factors affecting Performance of biofertilizers:• Host plant• Soil fertility• Cropping practices• Biological & environmental factors Survival and efficiency of BF are affected by the above factors 7
  8. 8. Rhizobium• Bacteria that grows in root nodules of legumes [soya bean, etc.]• Rhizobium INOCULANTS: Use of Rhizobium culture in legumes is most promising. On average, its use can supply 15-20 kg N/ha to legumes: increase yield up to 20 per cent. 8
  9. 9. 9
  10. 10. Rhizobium• Rhizobium bacteria in roots of legume plants fix atmospheric N2 in nodules formed on the roots of plants. A pure and efficient strain of Rhizobium multiplied in the lab on a suitable medium by using shake flask technology or fermentation technology and inoculated into seed or root. 10
  11. 11. 11
  12. 12. Inoculum Application 12
  13. 13. Inoculum Application 13
  14. 14. 14
  15. 15. Non-legume INOCULANTS Azotobacter# These bacteria enhance the plant growth and finally yield. A highly efficient strain _ Azotobacter chrooccocum is grown in the lab either as shake culture or using fermenter. 15
  16. 16. 16
  17. 17. Azotobacter:• Free living micro-organisms, that grow in the rhizosphere and fix atmospheric nitrogen non-symbiotically• Makes it available to particularly cereals. Promotes seed germination, initial vigor of plants- by producing growth producing substances 17
  18. 18. 18
  19. 19. 19
  20. 20. Azospirillum• Azospirillum, [ Azospirillum lipoferum, biological nitrogen fixing grasses; enhance biomass-root system]• Associative endo-symbiont on roots of grasses and similar types of plants. Also fixes atmospheric nitrogen and benefits host plants by supplying growth hormones and vitamins. 20
  21. 21. Azospirillum• Commonly used for preparation of commercial inoculants on a large scale, for cereals and grasses as it produces growth promoters in addition to fixing N2.• Has good adaptability to temp, soil pH and wide host range. 21
  22. 22. 22
  23. 23. Cyanobacteria INOCULANTS::• Biological nitrogen fixing ; photosynthetic also; Some free living cyanobacteria like Nostoc, Tolypothirix etc and other symbiotic like - Anabaena-Azolle_ useful for rice 23
  24. 24. CyanobacteriaAn important group of micro-organisms, fix atmospheric nitrogen non-symbiotically mostly in rice fields inheterocysts cells, specially known assites of nitrogen fixation. 24
  25. 25. Cyanobacteria: PreparationEfficient strains_ used for multiplication on a large scale in field. Production of inoculum in artificially controlled conditions is defined but more expensive. Open air soil culture_ most simple, less expensive_ easily adaptable by the farmers. 25
  26. 26. Cyanobacteria: Preparation– # Based on use of starter culture that is multi-strain inoculum of_ Aulosira, Tolypothrix, Scytonema, Nostoc and Anabaena.– # In rural areas, unskilled labour can undertake the multiplication of blue green algae as a paying industry. 26
  27. 27. Phosphate SOLUBILIZING INOCULANTS• Phosphate solubilizing microorganisms are Bacteria (Bacillus megaterium) and / or fungi (Aspergillus awamorii) -• Secrete organic acids - dissolve bound phosphate in soil 27
  28. 28. Phosphate Solubilizing bacteria / fungi:Phosphorus_ required for plant growth and water yield. It is also essential for nodulation by Rhizobium.– Phospho-micro organism are mostly bacteria and fungi. Mycorrhizae have high potential of phosphorus accummulation in plants. 28
  29. 29. VAM fungi [Vesicular Arbuscular Mycorrhiza]Intracellular, obligate, fungal endo- symbiont - glomus etc.- transfers phosphorus, zinc & sulfur from soil to root. 29
  30. 30. A schematic diagram of Vesiculararbuscular mycorrhiza 30
  31. 31. Mycorrhiza• Colonise 85% of land plants_ offers 50 % saving in chemical P fertilizer_ higher resistance to soil-and-root borne pathogens_ helps in wasteland reclamation by providing extended arm to plant root system. # A broad-spectrum mycorrhizal biofertilizer is available. 31
  32. 32. Mycorrhiza Produced by:# M/s Cadila PharamaceuticalLtd., Ahemdabad, and KCPSugar and Industries (Pvt.) Ltd.,Chennai [DBT-TERI, New Delhi’stechnology]. 32
  33. 33. Award for Producer of Mycorrhiza• KCP Sugar Industries received the All India Biotechnology Association Award for production and marketing of the mycorrhizal bio-fertilizer. 33
  34. 34. Estimated potential Demand for Biofertilisers by 2000-2001• Type of Biofertiliser • Demand (Tonnes)• Rhizobium • 34,999• Azotobacter • 145,953• Azospirillum • 74,342 • 251,738• Blue green Algae• Phosphate solublising • 255,340 microorganism 762,372• Total: 34
  35. 35. Biofertilizer Production Support: GOI- 1999-2000• National Biofertilizer Development Centre- established at Ghaziabad with 6 Regional BF Development Centres each at Jabalpur, Hissar, Nagpur, Bangalore, Bhubaneswar and Imphal.• Under this scheme 74 BF-production units established with central assistance having annual production capacity of 8475 tonnes. 35
  36. 36. Promoting Bio-fertilizers: Current Situation• A limited extent of success till date (2000).• There has been no accelerated growth in distribution with time.• Inadequate spatial diffusion.• Despite entry of small private units into the industry there is no clear indication of the success of privatization. 36
  37. 37. Progress of the bio-fertilizer Industry• Based on the data for 1995, 1997 and 1999, the industry witnessed a steady increase in the number of units producing the BF ;• the bulk of the growth took place by 1992-95 of the sample period and stagnated thereafter. 37
  38. 38. Changes in the share by type of BF• moderate success in AZT and by far the best performance by PSB• decline in RHZ indicated success in groundnut and pulses was below expectation. 38
  39. 39. 39
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  43. 43. Main constraints in spread of BF as an Industry:1. Inadequate production and supplyof efficient cultures of micro-organisms to farmers well beforesowing.2. Quality control aspect3. Lack of publicity, communication. 43
  44. 44. Marketing: Host, soil & climate specific BF: Receive user feedback• To cover the vast areas of legumes, pulses and oil seeds cereals and horticultural crops- to train users to apply and to receive feedback info. from them.• More manufacturer-distributors needed_ to prepare the bio-fertilizers in particular locality and supply to farmers as per farmer’s need, while earning profit. 44
  45. 45. Marketing of BF: Logistics• # Prepared bio-fertilizers should reach farmers prior to sowing. # If given free of cost, that lowers down the importance of product and farmers don’t use them carefully. # If bio-fertilizers are supplied after expiry date_expected results are not obtained. 45
  46. 46. Steps in biofertilizer production Strain selection, Mass Culture, Carrier preparation and Inoculation Quality testing. Packaging Distribution 46
  47. 47. NATURE OF BF INDUSTRY:• Indigenous technology• Scientific aspects: Standardized by Agricultural Universities and Research Labs.• Machineries and laboratory equipments are of BIS standards. 47
  48. 48. Manufacturing equipments:• Laboratory equipments,• Autoclaves,• Fermenter assembly,• Boiler,• Broth dispensers,• Plant for sterlisation, deminralising• Air compressor 48
  49. 49. The size of a Biofertiliser unit:• Capacity of production of various types / strains of bio-fertilizers per annum.• The projects so far set up in our country vary from 75 TPA to 300 TPA.• Expandable by adding a few additional equipment like a fermenter and / or adding another shift. 49
  50. 50. Requirements of BF Projects1 Land2 Layout and buildings3 Plant and Machinery4 Manufacturing process and Source of technology5 Infrastructural Facilities for raw material, carrier materialand utilities (Power, Water, Compressed air, Vehicles)6 Manpower Unit Size7 Business Prospects And Marketing and Selling 50Arrangements
  51. 51. 51
  52. 52. SOME OF BIO-FERTILIZERSIN THEMARKET 52
  53. 53. Dr Bhabani Dikshit 53
  54. 54. 54
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  57. 57. 57
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  63. 63. Extension work needed for popularizing BF• Visits to areas of undivided Puri district comprising of Pipli , Khurda and Nayagarh belt, showed that a large number of farmer of Chandanpur area applied biofertilizer in their betel vine crops by using Azatobactor and PSB (Phosphates soluble bacteria) and yielded good results. A senior Government agronomist of the Fertilizer Promotion and Agricultural Research Division (FP & ARD) based in Puri, Simanchala Panigrahi, said that as biofertilizer was a new concept to the farmers of Orissa, it needed further vigorous 63 extension work for popularizing its use.
  64. 64. 64
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  68. 68. BOOKS ABOUTBIOFERTILIZERS CONTINUED
  69. 69. Indian Books on Biofertilizers-1• Biofertilizer - Technology, Marketing and usage, a source book-cum- Glossary by Dr. M R Motsara et al (1995) - Fertilizer Development and Consultation Organization publishers, New Delhi 110048 (India) 69
  70. 70. Indian Books on Biofertilizers-2• Biofertilizers in Agriculture and Forestry by N S Subba Rao, Oxford & IBH, New Delhi.• Biotechnology of Biofertilizers Edited by S. Kannaiyan, Narosa Publishing House, N. Delhi, 2002 70
  71. 71. Indian Books on Biofertilizers -3• Recent Advances in Biofertilizer Technology/ Edited by A.K. Yadav, S. Ray Chaudhuri and M.R. Motsara, 2001, Society for Promotion and Utilisation of Resources and Technology, New Delhi. [Vedam Books]• Phosphatic Biofertilizers / Laxmi Lal. Udaipur, Agrotech Pub., 2002. 71
  72. 72. Handbook of Biofertilizers• Handbook of microbial biofertilizers, M. K. Rai, Routledge, 2006 - 579 pages,• Sharply focused, up-to-date information on microbial biofertilizers--including emerging options such as "Piriformospora indica" and "Matsutake" The Handbook of Microbial Biofertilizers provides in-depth coverage of all major microbial biofertilizers (rhizobia, arbuscular mycorrhizal fungi, and cyanobacterias well as new and emerging growth promoters (endophytes). 72
  73. 73. It examines the role of microbes in growth promotion,bioprotectors, and bioremidiators, and presents protocolsand practical strategies for using microbes in sustainableagriculture.An abundance of helpful charts, tables, and figures makecomplex information easy to access and understand.In this first-of-its-kind volume, contributors from 11countries and several continents address importantissues surrounding microbial biofertilizers, 73
  74. 74. … including: the rhizobium-host-arbuscular mycorrhizal"tripartite relationship, mycorrhiza as a disease suppresserand stress reducer mycorrhiza helping bacteria theimpact of functional groups of soil microorganisms onnutrient turnover PBPRs as biofertilizers andbiopesticides the potential of wild-legume rhizobia for useas a biofertilizers, the expanding role of blue-green algaein sustainable agriculture, the role of microbial fertilizersin sustainable plant production new and emergingendophytes the commercial potential of biofertilizers 74
  75. 75. In this young century, the use of biofertilizers is alreadygrowing rapidly. It has been recognized that theseenvironment-friendly bioprotectors, growth boosters, andremediators are essential for soil / plant health. TheHandbook of Microbial Biofertilizers is designed to fit theexpanding information needs of current and futurebiotechnologists, microbiologists, botanists, agronomists,environmentalists, and others whose work involvessustained agriculture. Handbook of Biofertilizers 75

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