Yersina Pestis may be isolated from the patient\'s faeces, blood, or vomit. The transmission of Y. pestis is by fleas Sheep blood agar (SBA) is a general bacteriologic medium used for the isolation and examination of colonial morphology of bacterial organisms It is a facultative anaerobic organism which is not fastidious and will grow well on any nutrient medium including SBA Yersinia pestis is a Gram-negative, bipolar-staining coccobacillus member of the Enterobacteriaceae family, and is an obligate intracellular pathogen that must be contained within the blood to survive. It is also a fermentative, motile organism that produces a thick anti- phagocytic slime layer in its path. Yersinia pestis encodes two antigenic molecules: Fraction 1 (F1) capsular antigen, and VW antigen. Both of these molecules are needed for pathogenicity, and are not expressed at temperatures lower than 37°C. Three forms of the plague that commonly occur worldwide due to Yersina Pestris are: bubonic, septicemic, and pneumonic . Antibiotics such as streptomycin, gentamicin, doxycycline, or ciprofloxacin are used to treat plague. Oxygen, intravenous fluids, and respiratory support usually are also needed. Patients with pneumonic plague should be strictly isolated from caregivers and other patients, (nytimes, 2011) Solution Yersina Pestis may be isolated from the patient\'s faeces, blood, or vomit. The transmission of Y. pestis is by fleas Sheep blood agar (SBA) is a general bacteriologic medium used for the isolation and examination of colonial morphology of bacterial organisms It is a facultative anaerobic organism which is not fastidious and will grow well on any nutrient medium including SBA Yersinia pestis is a Gram-negative, bipolar-staining coccobacillus member of the Enterobacteriaceae family, and is an obligate intracellular pathogen that must be contained within the blood to survive. It is also a fermentative, motile organism that produces a thick anti- phagocytic slime layer in its path. Yersinia pestis encodes two antigenic molecules: Fraction 1 (F1) capsular antigen, and VW antigen. Both of these molecules are needed for pathogenicity, and are not expressed at temperatures lower than 37°C. Three forms of the plague that commonly occur worldwide due to Yersina Pestris are: bubonic, septicemic, and pneumonic . Antibiotics such as streptomycin, gentamicin, doxycycline, or ciprofloxacin are used to treat plague. Oxygen, intravenous fluids, and respiratory support usually are also needed. Patients with pneumonic plague should be strictly isolated from caregivers and other patients, (nytimes, 2011).