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Leukocytes or WBC: They are of two types:
Granulocytes: Neutrophils, eosinohils, and basophils
Agranulocytes: Monocytes and lymphocytes
Neutrophils: MOst numberous of all the WBC, make 60 - 80% of WBC. Upon Giemsa staining,
the granules take up both eosin red and methylene blue, thus appear purple color. The cytoplasm
stains blue with multilobed nucleus. Their number increases in pathological conditions like
infectionss.
Eosinophils: upto 6% of total WBC, their granules take up Eosin red and appear red to pink
colored, due to eosinophilic cationic protein that binds the dye. Cytoplasm stains blue, bilobed
nucleus. The number increases with conditions like parasitic manifestations and viral infections.
Basophils: Generally absent or rarely found in healthy people. Their granules take up methylene
blue due to negatively charged molecles like heparin present in them. They are responsible for
certian types of hypersensitivities. Their number increaes during allergies.
Monocytes: They have a slightly indented nucleus that occupies about half of the cell. They are
larger than other cells. The cytoplasm shows granules but are not conspicuously stained. They
are cells that have the function of phagocytosis, next to neutrophils.
Lymphocytes: Large nucleus that occupies most of the cell leaving a thin rim of cytoplasm. They
produce antibodies.Nucleus stains purple and cytoplasm blue. Their number increases in
conditions like leukemia.
Solution
Leukocytes or WBC: They are of two types:
Granulocytes: Neutrophils, eosinohils, and basophils
Agranulocytes: Monocytes and lymphocytes
Neutrophils: MOst numberous of all the WBC, make 60 - 80% of WBC. Upon Giemsa staining,
the granules take up both eosin red and methylene blue, thus appear purple color. The cytoplasm
stains blue with multilobed nucleus. Their number increases in pathological conditions like
infectionss.
Eosinophils: upto 6% of total WBC, their granules take up Eosin red and appear red to pink
colored, due to eosinophilic cationic protein that binds the dye. Cytoplasm stains blue, bilobed
nucleus. The number increases with conditions like parasitic manifestations and viral infections.
Basophils: Generally absent or rarely found in healthy people. Their granules take up methylene
blue due to negatively charged molecles like heparin present in them. They are responsible for
certian types of hypersensitivities. Their number increaes during allergies.
Monocytes: They have a slightly indented nucleus that occupies about half of the cell. They are
larger than other cells. The cytoplasm shows granules but are not conspicuously stained. They
are cells that have the function of phagocytosis, next to neutrophils.
Lymphocytes: Large nucleus that occupies most of the cell leaving a thin rim of cytoplasm. They
produce antibodies.Nucleus stains purple and cytoplasm blue. Their number increases in
conditions like leukemia.

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Leukocytes or WBC They are of two typesGranulocytes Neutrophils.pdf

  • 1. Leukocytes or WBC: They are of two types: Granulocytes: Neutrophils, eosinohils, and basophils Agranulocytes: Monocytes and lymphocytes Neutrophils: MOst numberous of all the WBC, make 60 - 80% of WBC. Upon Giemsa staining, the granules take up both eosin red and methylene blue, thus appear purple color. The cytoplasm stains blue with multilobed nucleus. Their number increases in pathological conditions like infectionss. Eosinophils: upto 6% of total WBC, their granules take up Eosin red and appear red to pink colored, due to eosinophilic cationic protein that binds the dye. Cytoplasm stains blue, bilobed nucleus. The number increases with conditions like parasitic manifestations and viral infections. Basophils: Generally absent or rarely found in healthy people. Their granules take up methylene blue due to negatively charged molecles like heparin present in them. They are responsible for certian types of hypersensitivities. Their number increaes during allergies. Monocytes: They have a slightly indented nucleus that occupies about half of the cell. They are larger than other cells. The cytoplasm shows granules but are not conspicuously stained. They are cells that have the function of phagocytosis, next to neutrophils. Lymphocytes: Large nucleus that occupies most of the cell leaving a thin rim of cytoplasm. They produce antibodies.Nucleus stains purple and cytoplasm blue. Their number increases in conditions like leukemia. Solution Leukocytes or WBC: They are of two types: Granulocytes: Neutrophils, eosinohils, and basophils Agranulocytes: Monocytes and lymphocytes Neutrophils: MOst numberous of all the WBC, make 60 - 80% of WBC. Upon Giemsa staining, the granules take up both eosin red and methylene blue, thus appear purple color. The cytoplasm stains blue with multilobed nucleus. Their number increases in pathological conditions like infectionss. Eosinophils: upto 6% of total WBC, their granules take up Eosin red and appear red to pink colored, due to eosinophilic cationic protein that binds the dye. Cytoplasm stains blue, bilobed nucleus. The number increases with conditions like parasitic manifestations and viral infections. Basophils: Generally absent or rarely found in healthy people. Their granules take up methylene blue due to negatively charged molecles like heparin present in them. They are responsible for certian types of hypersensitivities. Their number increaes during allergies.
  • 2. Monocytes: They have a slightly indented nucleus that occupies about half of the cell. They are larger than other cells. The cytoplasm shows granules but are not conspicuously stained. They are cells that have the function of phagocytosis, next to neutrophils. Lymphocytes: Large nucleus that occupies most of the cell leaving a thin rim of cytoplasm. They produce antibodies.Nucleus stains purple and cytoplasm blue. Their number increases in conditions like leukemia.