Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Anti-Phospho-Cofilin (S3) -http://www.stjohnslabs.com/phospho-cofilin-s3-antibody Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

1. Rating:
Specificity:
ANTIBODY CUSTOMER REVIEW
Findings: “It is a good antibody for
western blotting” – External Lab
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Protocol
Gel
Electrophoresis
15% SDS-PAGE gel to run
samples.
Membrane
Wash
Washed with PBS +0.05% TWEEN
20 for four time
Transfer Transfer to PVDF membrane. Secondary
Antibody
Probing
Secondary antibody HRP
conjugated anti-rabbit 1:5000 for
1 hour at RT.
Blocking 5% Milk Membrane
Wash
Washed with PBS +0.05% TWEEN
20 for four time
Primary
Antibody
Probing
Primary antibody, dilution with
1:20000, incubate overnight.
Visualisation ECL visualization was undertaken
after 5 sec, 10 sec, 1 min, 5min,
20 min and 1hr.
Model Number STJ90230
Antibody Name Anti-Phospho-Cofilin (S3)
antibody
Application Western Blot
Species Human
Tissue HEK293T
Dilution 1:20000
Secondary Antibody HRP conjugated anti-
rabbit
Secondary Dilution 1:5000
Fig 1. we used this antibody to detect p-cofilin in
HEK293T cells and could detect a band around
18-19 KD.