Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum. Anti-Endophilin I -http://www.stjohnslabs.com/endophilin-i-antibody Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

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Specificity:
ANTIBODY CUSTOMER REVIEW
Findings: “Antibody is giving bands of
Endophilin-I with multiple non specific band or
different isoforms in lymphoma cell lines.” –
External Laboratory.
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Protocol
Gel
Electrophoresis
6% polyacrylamide gel,
constant voltage 20 V,
overnight.
Membrane
Wash
3 times 1X TBST wash for 5
minutes.
Transfer 0.45 nm nitrocellulose
membrane, 1 piece of gel (wet
transfer), constant voltage 20 V
overnight.
Secondary
Antibody
Probing
Secondary antibody was added to
the solution of 1X TBST with 5%
BSA with 1:2500 dilution ratio,
for 2 hr.
Blocking 1X TBST with 5% Bovine serum
albumin (BSA) for 2 hr.
Membrane
Wash
5 times 1X TBST wash for 5
minutes.
Primary
Antibody
Probing
Primary antibody was added to
the solution of 1X TBST with
5% BSA, 4˚C for overnight.
Visualisation ECL visualization for 1 min.
Model Number STJ92924
Antibody Name Anti-Endophilin I
antibody
Application Western Blot
Species Human
Tissue LY3, JEKO, Granta
Dilution 1:1000
Secondary Antibody Amersham ECL Rabbit
IgG, HRP-linked whole
Ab (from donkey)
Secondary Dilution 1:2500
Fig. Figure showing the Bands of Endophilin-I or SH3GL2. It also may
have multiple isoforms of SH3GL2.