Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. To purchase this antibody, use the following link: http://www.stjohnslabs.com/actin-b-antibody?filter_name=STJ91464

1. HOW DID OUR
ANTIBODY PERFORM?
CUSTOMER REVIEW: Actin-beta Polyclonal Antibody (STJ91464)

2. WHAT WAS USED?
Primary antibody:
Product Catalog No: STJ91464. Actin-beta polyclonal
antibody. 100µl
Provider: St John's Laboratory
Dilution ratio: 1:1000
Secondary antibody:
HRP goat anti-rabbit (provided by BPI)
Reference antibody:
Actin (provided by BPI)

3. WHAT WAS THE PROTOCOL?
Antibody: STJ91464. Actin-beta antibody
Method for validation: Western Blot
Materials for validation: HeLa cell line, Jurkat
cell line
Treatment of materials: When cells were cultured
to 70%-80% plating density, lysis buffer with
protease inhibitors (Sigma) and equal volume of
2X loading buffer were added for 10 min water
bath at 100˚C.

4. WHAT WAS THE PROTOCOL?
Gel electrophoresis: 10% polyacrylamide gel, constant voltage 160 V, 60 min.
Transfer: 0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant
voltage 100 V 60 min.
Blocking: 1X TBST with 5% skimmed milk powder for 2 hr.
Primary antibody probing: primary antibody was added to the solution of 1X
TBST with 5% skimmed milk powder, 4˚C for overnight.
Membrane wash: 1X TBST wash for 3 times.
Secondary antibody probing: secondary antibody was added to the solution of 1X
TBST with 5% skimmed milk powder with 1:10000 dilution ratio, for 1.5 hr.
Membrane wash: 1X TBST wash for 3 times.
Visualization: ECL visualization for 5 min (the visualization time was
determined by actual result. It was ensured that the visualization time was
identical for the same antigen).

5. WHAT WAS THE PROTOCOL?
Gel electrophoresis information：10% polyacrylamide gel, constant voltage 160 V, 60 min.
Transfer information：0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant voltage 100 V 60
min.
No. Antigen
Loading
amount
Primary
antibody
Primary
antibody
dilution
ratio
Secondary
antibody
dilution
ratio
Target band
KD
Visualization
time
1 HeLa lysate 10 µl STJ91464.
Actin-beta
antibody
1:1000
1:10000 50KD 20S
2
Jurkat
lysate
10 µl 1:10000 50KD 20S

6. WHAT DID THE CUSTOMER THINK?
Testing results were provided by BPI laboratory. They
stated that “In HeLa and Jurkat cell line, there were target
bands detected by STJ91464 Actin-beta Antibody”.

7. “WE INVITE YOU TO TEST OUR PRODUCTS”
Our aim is to not only connect our customers
with the best product, but to also give them
the opportunity to test the product’s
performance risk free.

8. Contact Us: info@stjohnslabs.com
1. Choose 5 Trial Size Samples from our free sample list
2. Complete the free sample application: http://www.stjohnslabs.com/free-sample
3. Receive your antibody
4. Submit your review
5. Receive your 30% off your next order
Get Your Free Samples in 5 Easy Steps

9. Follow Us for Weekly Independent
Validation Reviews
@StJohnsLabsSt John's Laboratory Ltd
St John's Laboratory Ltd St John's Laboratory Ltd
http://www.slideshare.net/stjohnslabs