Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis. To purchase this antibody, use the following link: http://www.stjohnslabs.com/epha2-antibody-p-96094?filter_name=STJ92940

1. HOW DID OUR
ANTIBODY PERFORM?
CUSTOMER REVIEW: EphA2 Polyclonal Antibody (STJ92940)

2. WHAT WAS USED?
Primary antibody: STJ92940 EphA2 Polyclonal Antibody
Provider: St John's Laboratory
Dilution ratio: 1:1000
Application: Western Blot.
Image: In MDA-MB-231 cell line, there were target bands
detected by STJ92940 EphA2 antibody.
250
150
100
75
50
37
25
Marker
MDA-MB-231
cells

3. WHAT WAS THE PROTOCOL?
Gel electrophoresis: 9% polyacrylamide gel, constant voltage 80 mA, 60 min.
Transfer: 0.45 µm nitrocellulose membrane, 1 piece of gel (wet transfer), constant
voltage 100 V 75 min.
Blocking: 1X TBST with 5% skimmed milk powder for 1 hr.
Primary antibody probing: primary antibody was added to the solution of 1X
TBST with 5% BSA, 4˚C for overnight.
Membrane wash: 1X TBST wash for 3 times.
Secondary antibody probing: Goat anti-rabbit HRP-linked secondary antibody was
added to the solution of 1X TBST with 5% skimmed milk powder with 1:2500
dilution ratio, for 1 hr.
Membrane wash: 1X TBST wash for 4 times.
Visualization: ECL visualization for 5s (the visualization time was determined by
actual result. It was ensured that the visualization time was identical for the same
antigen).
250
150
100
75
50
37
25
Marker
MDA-MB-231
cells

4. WHAT WAS THE PROTOCOL?
Gel electrophoresis information：10% polyacrylamide gel, constant voltage 160 V, 60 min.
Transfer information：0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant voltage 100 V 60
min.
Antigen
Loading
amount
Primary
antibody
Primary
antibody
dilution
ratio
Secondary
antibody
dilution ratio
Target band KD
Visualization
time
MDA-MB-231
lysate
10 µg
STJ92940
EphA2Poly
clonal
Antibody
1:1000 1:2500 103KD 5s
250
150
100
75
50
37
25
Marker
MDA-MB-231
cells

5. WHAT DID THE CUSTOMER THINK?
Testing results were provided by Sri HariKrishna Vellanki
from Royal College of Surgeons Ireland. They stated that “In
MDA-MB-231 cell line, there were target bands detected by
STJ92940 EphA2 antibody.”

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Our aim is to not only connect our customers
with the best product, but to also give them
the opportunity to test the product’s
performance risk free.

7. Contact Us: info@stjohnslabs.com
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2. Complete the free sample application: http://www.stjohnslabs.com/free-sample
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