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Workplace Drug Screening Using LCMSMS Grant Moore Workplace Drug Screening Canterbury Health Labs
Overview ,[object Object],[object Object],[object Object],[object Object]
A Spouse ’ s Perspective ,[object Object],[object Object],[object Object]
Workplace Drug Testing Standard ,[object Object],[object Object],[object Object]
AS/NZS-4308 Testing Standard ,[object Object],[object Object],[object Object]
Additions to the Standard - 2008
Additions to the Standard ,[object Object],[object Object]
Workplace Testing Standard ,[object Object],[object Object],[object Object]
POCT devices iScreen One step drug card Inverness Medical Sure Step6 Panel cassette Thermo Fisher Scientific Micro Tox Multi Drug Screening Test Branan QuickTox Bio-Rad Tox/See
Workplace Testing Standard – Immunoassay cut-offs
Immunoassay Screening ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Immunoassay analysers ,[object Object]
Workplace Testing Standard – Confirmation cut-offs
Confirmation Compounds ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
ABI 3200 Qtrap LCMSMS
… and after the Earthquake! …  it still worked!
LCMSMS – Some terminology! ,[object Object],[object Object],[object Object],[object Object],[object Object]
LCMSMS – more terminology! ,[object Object],[object Object],[object Object],[object Object],[object Object]
MS Components
So how does it work? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
So how does it work? Q1 Q2 Q3
So how much sample do we need?
So how much sample do we need? ,[object Object],[object Object],[object Object],[object Object],[object Object]
So what can we detect/confirm? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Amphetamine-type substances ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Amphetamine MDMA
Amphetamine-type substances ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Methcathinone p-Methoxyamphetamine
Benzodiazepine metabolites ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Diazepam Oxazepam
Benzodiazepines ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Tetrahydrocannabinol (THC) Δ 9 -Tetrahydrocannabinol ,[object Object],[object Object],[object Object],[object Object]
Opiates ,[object Object],[object Object],[object Object],[object Object]
Opiate metabolism
Synthetic Cannabinoids ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
What is the significance of JWH? ,[object Object],[object Object],[object Object],[object Object]
HU-210 ,[object Object],[object Object],[object Object]
Structural Similarities? Tetrahydrocannabinol
JWH-018 metabolites
Are there Cross-Reactivity issues? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Synthetic Cannabinoids Method ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Establishing a method
Procedure ,[object Object],[object Object],[object Object],[object Object]
Results – blank samples
Kronic negative
Kronic positive
How prevalent is Kronic use? ,[object Object],[object Object],[object Object],[object Object],[object Object]
Where to from here? ,[object Object],[object Object],[object Object],[object Object]
Kronic selection
POCT EQA Programme ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
OUTS Program ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Cannabis End of Cycle report
Cannabis EOC Report ,[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Cannabis EOC
Methamphetamine EOC
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Methamphetamine EOC
POCT EQA ,[object Object],[object Object],[object Object],[object Object]
POCT EQA Scheme
Some reflections on the past year!
Acknowledgements ,[object Object],[object Object]

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Workplace Drug Screening Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MSMS)

Editor's Notes

  1. The material was manufactured by Australian Scientific Enterprises as Human urine specimens 6 linear concentration levels – a low and a high pool with 4 intermediate levels In setting the specifications for all the drugs we used the AS/NZS:4308 standard cut-off limits to offer a range of concentrations from 0ug/L through to significantly above the cut-off value for each drug This year and again in 2011 laboratories can purchase either a 1ml or 10ml vial depending on the sample volume required for analysis The program consists of a single 12 month cycle January through to December and 1 sample per month The program has a specifically designed report for each participant to evaluate their results according to the cut-offs for their device The target values for each drug for every concentration level were set using the standard QAP target setting statistical system suing GCMS and LCMSMS methods