Wasp sting, wasp venom composition, immune response, local reactions, severe reactions, anaphylaxis, treatment with home remedies and OTC products, rational treatment with topical phytochemicals.
A 14-year-old boy presented to the emergency department with symptoms of an autonomic storm after being bitten by a scorpion. He was diagnosed with scorpion sting in autonomic storm. He was given prazosin, hydrocortisone, and other supportive treatments. His condition stabilized and he was discharged after 6 days. Scorpion stings can cause local effects and a systemic autonomic storm response due to neurotoxins that affect sodium channels and induce catecholamine release. Prazosin is an effective treatment as it blocks alpha receptors and counters the effects of venom.
Organophosphorus compounds are widely used as pesticides and chemical weapons. They work by inhibiting acetylcholinesterase, leading to accumulation of acetylcholine and overstimulation of nicotinic and muscarinic receptors. Clinical features include excessive secretions, nausea, vomiting, diarrhea, weakness and respiratory failure. Treatment involves decontamination, atropine to block muscarinic effects, pralidoxime to reactivate acetylcholinesterase, and supportive care. Prognosis depends on prompt diagnosis and treatment, with mortality risks highest within 24 hours from respiratory or cardiac failure.
Scorpion stings are a major public health problem in tropical countries. The document discusses the clinical presentation, pathophysiology, grading, investigations, and management of scorpion stings. It focuses on the Indian red scorpion Mesobuthus tamulus, which can cause a life-threatening hypertensive crisis and pulmonary edema if not treated promptly. Prazosin is the primary treatment to counteract venom effects and prevent complications. Scorpion antivenom may also be given if autonomic symptoms persist. Supportive care includes fluid resuscitation, oxygen therapy, diuretics and inotropes as needed.
Snakebite is a significant public health issue in India, causing approximately 50,000 deaths per year. The document discusses snakebite as an occupational hazard, particularly for agricultural workers. It provides details on the types of poisonous snakes found in India, the symptoms and complications of snakebites, and the current treatment protocol. The protocol involves first aid, diagnosis, and treatment with antivenom immunotherapy. Improving access to timely medical care and antivenom could help reduce the high snakebite mortality in India.
1) Paracetamol is metabolized in the liver and can cause toxicity in high doses by depleting glutathione levels, allowing the reactive metabolite NAPQI to damage cells.
2) For paracetamol overdose, N-acetylcysteine should be administered within 8-10 hours as an antidote to replenish glutathione levels. The treatment involves intravenous administration over 21 hours.
3) Management also involves monitoring liver function tests and providing supportive care. Liver transplantation may be considered for severe liver failure due to paracetamol poisoning.
Snake bites can be fatal, causing around 30-40 thousand deaths per year in tropical countries. Poisonous snakes use venom glands and hollow fangs to inject victims. Cobra venom causes symptoms within minutes like blurred vision and paralysis, while krait and viper venom have longer onset but can cause bleeding, organ damage, and death from shock. First aid involves cleaning the wound, immobilizing the limb, and rushing the victim to the hospital. There, antivenom treatment, supportive care, and monitoring for anaphylaxis are given to counter the effects of the venom and save the victim's life.
- Hymenoptera stings (from bees, wasps, hornets) can cause localized swelling, redness, itching, and pain at the sting site. They may also cause a delayed hypersensitivity reaction with more swelling.
- For acute stings, scrape away the stinger, check for signs of allergic reaction, apply a cold pack, and observe the patient for an hour. Reassure patients seen after 12-24 hours that an allergic reaction is unlikely.
- For swelling, prescribe antihistamines. If lymphangitis is present, prescribe antibiotics. Elevate and splint swollen extremities. Do not apply heat or belittle the patient.
This document discusses snake poisoning from venomous snake bites. It begins by classifying snakes as either poisonous or non-poisonous, and further classifying poisonous snakes. The symptoms and treatment of bites from neurotoxic, vasculotoxic, and myotoxic snakes are described. Treatment involves first aid measures to prevent venom spread, use of antivenom to neutralize toxins, and supportive care. Postmortem findings are also outlined.
A 14-year-old boy presented to the emergency department with symptoms of an autonomic storm after being bitten by a scorpion. He was diagnosed with scorpion sting in autonomic storm. He was given prazosin, hydrocortisone, and other supportive treatments. His condition stabilized and he was discharged after 6 days. Scorpion stings can cause local effects and a systemic autonomic storm response due to neurotoxins that affect sodium channels and induce catecholamine release. Prazosin is an effective treatment as it blocks alpha receptors and counters the effects of venom.
Organophosphorus compounds are widely used as pesticides and chemical weapons. They work by inhibiting acetylcholinesterase, leading to accumulation of acetylcholine and overstimulation of nicotinic and muscarinic receptors. Clinical features include excessive secretions, nausea, vomiting, diarrhea, weakness and respiratory failure. Treatment involves decontamination, atropine to block muscarinic effects, pralidoxime to reactivate acetylcholinesterase, and supportive care. Prognosis depends on prompt diagnosis and treatment, with mortality risks highest within 24 hours from respiratory or cardiac failure.
Scorpion stings are a major public health problem in tropical countries. The document discusses the clinical presentation, pathophysiology, grading, investigations, and management of scorpion stings. It focuses on the Indian red scorpion Mesobuthus tamulus, which can cause a life-threatening hypertensive crisis and pulmonary edema if not treated promptly. Prazosin is the primary treatment to counteract venom effects and prevent complications. Scorpion antivenom may also be given if autonomic symptoms persist. Supportive care includes fluid resuscitation, oxygen therapy, diuretics and inotropes as needed.
Snakebite is a significant public health issue in India, causing approximately 50,000 deaths per year. The document discusses snakebite as an occupational hazard, particularly for agricultural workers. It provides details on the types of poisonous snakes found in India, the symptoms and complications of snakebites, and the current treatment protocol. The protocol involves first aid, diagnosis, and treatment with antivenom immunotherapy. Improving access to timely medical care and antivenom could help reduce the high snakebite mortality in India.
1) Paracetamol is metabolized in the liver and can cause toxicity in high doses by depleting glutathione levels, allowing the reactive metabolite NAPQI to damage cells.
2) For paracetamol overdose, N-acetylcysteine should be administered within 8-10 hours as an antidote to replenish glutathione levels. The treatment involves intravenous administration over 21 hours.
3) Management also involves monitoring liver function tests and providing supportive care. Liver transplantation may be considered for severe liver failure due to paracetamol poisoning.
Snake bites can be fatal, causing around 30-40 thousand deaths per year in tropical countries. Poisonous snakes use venom glands and hollow fangs to inject victims. Cobra venom causes symptoms within minutes like blurred vision and paralysis, while krait and viper venom have longer onset but can cause bleeding, organ damage, and death from shock. First aid involves cleaning the wound, immobilizing the limb, and rushing the victim to the hospital. There, antivenom treatment, supportive care, and monitoring for anaphylaxis are given to counter the effects of the venom and save the victim's life.
- Hymenoptera stings (from bees, wasps, hornets) can cause localized swelling, redness, itching, and pain at the sting site. They may also cause a delayed hypersensitivity reaction with more swelling.
- For acute stings, scrape away the stinger, check for signs of allergic reaction, apply a cold pack, and observe the patient for an hour. Reassure patients seen after 12-24 hours that an allergic reaction is unlikely.
- For swelling, prescribe antihistamines. If lymphangitis is present, prescribe antibiotics. Elevate and splint swollen extremities. Do not apply heat or belittle the patient.
This document discusses snake poisoning from venomous snake bites. It begins by classifying snakes as either poisonous or non-poisonous, and further classifying poisonous snakes. The symptoms and treatment of bites from neurotoxic, vasculotoxic, and myotoxic snakes are described. Treatment involves first aid measures to prevent venom spread, use of antivenom to neutralize toxins, and supportive care. Postmortem findings are also outlined.
Management of Opioid Analgesic OverdoseSun Yai-Cheng
This document summarizes the management of opioid analgesic overdoses. It notes that opioid overdoses can have life-threatening effects on multiple organ systems. The duration of action varies between opioid formulations and an overdose can prolong intoxication. Prescriptions for opioid analgesics in the US increased 700% from 1997-2007. Opioid overdoses lead to over 27,500 health care facility admissions in 2010. Clinical signs of overdose include respiratory depression, apnea, miosis, and stupor. Naloxone is the antidote and works by reversing opioid receptor activity but has a shorter duration than many opioids. Higher and repeated naloxone doses may be needed for long-acting opioids like
Dr. Sachin Verma is a young, diligent and dynamic physician. He did his graduation from IGMC Shimla and MD in Internal Medicine from GSVM Medical College Kanpur. Then he did his Fellowship in Intensive Care Medicine (FICM) from Apollo Hospital Delhi. He has done fellowship in infectious diseases by Infectious Disease Society of America (IDSA). He has also done FCCS course and is certified Advance Cardiac Life support (ACLS) and Basic Life Support (BLS) provider by American Heart Association. He has also done a course in Cardiology by American College of Cardiology and a course in Diabetology by International Diabetes Centre. He specializes in the management of Infections, Multiorgan Dysfunctions and Critically ill patients and has many publications and presentations in various national conferences under his belt. He is currently working in NABH Approved Ivy super-specialty Hospital Mohali as Consultant Intensivists and Physician.
Rodenticides are chemicals used to kill rodent pests that can destroy large amounts of grains. They are commonly used but also pose risks to humans and pets through secondary poisoning. Common rodenticides include inorganic preparations like phosphorus and organic preparations like fluoroacetate compounds. Symptoms depend on the specific rodenticide ingested or inhaled and can include abdominal pain, vomiting, seizures, and bleeding. Treatment involves decontamination, supportive care, and antidotes like vitamin K for anticoagulants.
The document discusses bee sting management. It describes the different types of reactions to bee stings including mild local reactions, strong local reactions, and allergic reactions. It outlines treatments for minor reactions like removing the stinger, applying a cold compress, and taking an antihistamine. For moderate reactions, it recommends additional treatments like elevating the affected area, applying hydrocortisone cream, and oral antihistamines. Emergency treatment for allergic reactions may include epinephrine, oxygen, IV medications, and CPR if needed. The document also provides tips to avoid bee stings and sample multiple choice questions.
A 55-year-old male presented to the emergency room with swelling and numbness in his left leg after being bitten by a snake while gardening that morning. Tests revealed decreased hemoglobin and platelets, elevated coagulation markers, and electrolyte abnormalities indicating disseminated intravascular coagulation (DIC) from the snake bite. He was given oxygen, fluids, steroids, tetanus shot, anti-venom, and blood products to treat the DIC but his condition deteriorated and he died after being transferred to another hospital. The take-home message is that abnormal or prolonged bleeding after a snake bite could indicate DIC.
Paraquat poisoning causes severe oxidative stress and multi-organ failure. Ingestion has a high fatality rate. It is rapidly absorbed and concentrated in lungs, liver, and kidneys, causing cellular damage through redox cycling. Clinical features include oropharyngeal burns, respiratory distress, and acute kidney injury. Diagnosis is confirmed by positive urine dithionite test showing blue color change. Prognosis is poor for those with serum paraquat levels above the Sipp score threshold or rapidly increasing creatinine. Early extracorporeal removal within 4 hours may help severe cases, but long-term outcomes are generally poor with progressive lung fibrosis. Management focuses on supportive care, though antioxidants have been tried with
The document discusses oleandrin, the main toxic compound found in the plant Nerium oleander. It provides background on oleandrin's toxicity, structure, mechanism of action, and medical importance. While oleandrin is highly toxic and can be fatal, it is also under investigation for potential uses in cancer treatment and neuroprotection. However, its narrow therapeutic range and potential for drug interactions pose issues. The document also covers oleandrin's traditional uses in folk medicine, industrial applications, and current research developments regarding its medical potential.
A 30-year-old unconscious housewife was brought to the hospital with a history of domestic violence. On examination, she had constricted pupils, low blood pressure, high blood sugar, metabolic acidosis on ABG, bradycardia on ECG, and elevated serum amylase. The diagnosis was organophosphorus poisoning based on the clinical features and laboratory findings.
This document provides information about scorpion stings, including:
1) Scorpions have a lobster-like body with claws, legs, and a segmented tail ending in a stinger containing venom glands. Their venom causes uncontrolled nerve firing through sodium channel effects.
2) Symptoms range from localized pain to cranial nerve dysfunction and autonomic/muscular symptoms. Severe cases can lead to complications like respiratory failure.
3) Treatment involves wound care, pain management, antivenom if available, and supportive care like intubation, IV fluids and medication for symptoms. Outcomes are generally good with treatment.
Aluminum phosphide poisoning is a common lethal poisoning that has caused many deaths in India in recent decades. Aluminum phosphide is marketed as pesticide pellets or tablets used to preserve food grains. When it reacts with water, it produces phosphine gas which is toxic as it inhibits cellular respiration. Symptoms include vomiting, hypotension, and organ damage. Diagnosis involves detecting phosphine in breath or vomit. Treatment focuses on managing shock through fluids and vasopressors, as there is no antidote. Prevention requires better regulation of aluminum phosphide distribution to limit accidental or intentional ingestion.
This document provides guidance on snake bites in India. It discusses that snake bites are a major public health issue, killing over 11,000 people annually. The most common venomous snakes in India are Russell's viper, hump-nosed viper, cobra, and krait. It outlines the clinical presentation of envenomings from different snakes, including neuroparalytic effects from cobras and kraits, bleeding disorders from vipers, and muscle damage from sea snakes. The document emphasizes rapid assessment, resuscitation, detailed examination to identify the snake species, laboratory tests, and antivenom treatment for snake bites in India.
Snake Bite,Rabies,Scorpion Bite PPT – Presented By Prof.Dr.R.R.deshpande on 1...rajendra deshpande
This document provides information on snake bite treatment from an expert, Prof. Dr. R.R. Deshpande. It discusses the types of poisonous snakes in India and their distinctive features. It describes symptoms of neurotoxic and haemotoxic snake bites. The recommended treatment involves immobilizing the bitten area, applying ice or tourniquet, monitoring for symptoms, and administering Antivenom Serum intravenously if symptoms appear. It provides dosage guidelines and monitoring advice for different clinical presentations. Hospital admission is recommended for patients with bleeding, black urine or extensive paralysis.
The document outlines guidelines for the treatment of snake bites, including rapidly assessing the airway, breathing, and circulation of the patient; administering tetanus toxoid, antibiotics, and anti-venom as needed based on symptoms; and closely monitoring vital signs and administering supportive care for any complications like respiratory failure, hypotension, or renal failure. Laboratory tests are also recommended to identify coagulopathies or tissue damage from the venom.
This document summarizes the pathophysiology, clinical presentation, management, and prognosis of kerosene poisoning in children. Kerosene easily penetrates the lungs due to its low viscosity and surface tension, causing chemical pneumonitis that can lead to respiratory failure. Symptoms include cough, tachypnea, hypoxemia, and neurological effects. Management involves supportive care, with intubation and ventilation for severe cases. Gastric decontamination methods are not recommended due to risk of aspiration. Outcomes are generally good with supportive care alone, though extracorporeal membrane oxygenation or high frequency ventilation may be life-saving in severe cases unresponsive to conventional ventilation.
Bee and wasp stings can cause local reactions like pain, swelling and itching at the sting site. For some people, multiple stings or an allergic reaction can be life-threatening. Honeybees leave their stinger behind when they sting while wasps have a smooth stinger that can sting repeatedly. Symptoms of a local reaction include pain, swelling and hives near the sting that last up to a week. Removing the stinger, applying ice, taking antihistamines and pain relievers can help treat reactions. Wearing protective clothing and avoiding startling insects can help prevent stings.
This document provides information on corrosive poisoning, including types, mechanisms of injury, clinical features, investigations, management, and treatment. It discusses three main types of corrosives - acids, alkalis, and oxidants. Alkalis are noted as the most dangerous due to their ability to rapidly cause liquifactive necrosis and injury. Clinical features involve the gastrointestinal tract, respiratory system, eyes and skin. Investigations include endoscopy, imaging, and labs. Management focuses on airway protection, dilution, antibiotics, and monitoring. Long term complications like stricture formation may require repeated dilations.
This document provides an overview of immunity, including the basic concepts of innate and acquired immunity. It discusses the types of innate immunity like species, racial, and individual immunity. The mechanisms of innate immunity include anatomical barriers, phagocytic barriers, blood proteins, cytokines, inflammation, and fever. Acquired immunity is specific and involves the generation of immunological memory. It can be active, developed from natural infection or artificial vaccination, or passive, developed from maternal antibodies or antibody administration. The document also covers factors affecting individual innate immunity like age, hormones, and nutrition.
Management of Opioid Analgesic OverdoseSun Yai-Cheng
This document summarizes the management of opioid analgesic overdoses. It notes that opioid overdoses can have life-threatening effects on multiple organ systems. The duration of action varies between opioid formulations and an overdose can prolong intoxication. Prescriptions for opioid analgesics in the US increased 700% from 1997-2007. Opioid overdoses lead to over 27,500 health care facility admissions in 2010. Clinical signs of overdose include respiratory depression, apnea, miosis, and stupor. Naloxone is the antidote and works by reversing opioid receptor activity but has a shorter duration than many opioids. Higher and repeated naloxone doses may be needed for long-acting opioids like
Dr. Sachin Verma is a young, diligent and dynamic physician. He did his graduation from IGMC Shimla and MD in Internal Medicine from GSVM Medical College Kanpur. Then he did his Fellowship in Intensive Care Medicine (FICM) from Apollo Hospital Delhi. He has done fellowship in infectious diseases by Infectious Disease Society of America (IDSA). He has also done FCCS course and is certified Advance Cardiac Life support (ACLS) and Basic Life Support (BLS) provider by American Heart Association. He has also done a course in Cardiology by American College of Cardiology and a course in Diabetology by International Diabetes Centre. He specializes in the management of Infections, Multiorgan Dysfunctions and Critically ill patients and has many publications and presentations in various national conferences under his belt. He is currently working in NABH Approved Ivy super-specialty Hospital Mohali as Consultant Intensivists and Physician.
Rodenticides are chemicals used to kill rodent pests that can destroy large amounts of grains. They are commonly used but also pose risks to humans and pets through secondary poisoning. Common rodenticides include inorganic preparations like phosphorus and organic preparations like fluoroacetate compounds. Symptoms depend on the specific rodenticide ingested or inhaled and can include abdominal pain, vomiting, seizures, and bleeding. Treatment involves decontamination, supportive care, and antidotes like vitamin K for anticoagulants.
The document discusses bee sting management. It describes the different types of reactions to bee stings including mild local reactions, strong local reactions, and allergic reactions. It outlines treatments for minor reactions like removing the stinger, applying a cold compress, and taking an antihistamine. For moderate reactions, it recommends additional treatments like elevating the affected area, applying hydrocortisone cream, and oral antihistamines. Emergency treatment for allergic reactions may include epinephrine, oxygen, IV medications, and CPR if needed. The document also provides tips to avoid bee stings and sample multiple choice questions.
A 55-year-old male presented to the emergency room with swelling and numbness in his left leg after being bitten by a snake while gardening that morning. Tests revealed decreased hemoglobin and platelets, elevated coagulation markers, and electrolyte abnormalities indicating disseminated intravascular coagulation (DIC) from the snake bite. He was given oxygen, fluids, steroids, tetanus shot, anti-venom, and blood products to treat the DIC but his condition deteriorated and he died after being transferred to another hospital. The take-home message is that abnormal or prolonged bleeding after a snake bite could indicate DIC.
Paraquat poisoning causes severe oxidative stress and multi-organ failure. Ingestion has a high fatality rate. It is rapidly absorbed and concentrated in lungs, liver, and kidneys, causing cellular damage through redox cycling. Clinical features include oropharyngeal burns, respiratory distress, and acute kidney injury. Diagnosis is confirmed by positive urine dithionite test showing blue color change. Prognosis is poor for those with serum paraquat levels above the Sipp score threshold or rapidly increasing creatinine. Early extracorporeal removal within 4 hours may help severe cases, but long-term outcomes are generally poor with progressive lung fibrosis. Management focuses on supportive care, though antioxidants have been tried with
The document discusses oleandrin, the main toxic compound found in the plant Nerium oleander. It provides background on oleandrin's toxicity, structure, mechanism of action, and medical importance. While oleandrin is highly toxic and can be fatal, it is also under investigation for potential uses in cancer treatment and neuroprotection. However, its narrow therapeutic range and potential for drug interactions pose issues. The document also covers oleandrin's traditional uses in folk medicine, industrial applications, and current research developments regarding its medical potential.
A 30-year-old unconscious housewife was brought to the hospital with a history of domestic violence. On examination, she had constricted pupils, low blood pressure, high blood sugar, metabolic acidosis on ABG, bradycardia on ECG, and elevated serum amylase. The diagnosis was organophosphorus poisoning based on the clinical features and laboratory findings.
This document provides information about scorpion stings, including:
1) Scorpions have a lobster-like body with claws, legs, and a segmented tail ending in a stinger containing venom glands. Their venom causes uncontrolled nerve firing through sodium channel effects.
2) Symptoms range from localized pain to cranial nerve dysfunction and autonomic/muscular symptoms. Severe cases can lead to complications like respiratory failure.
3) Treatment involves wound care, pain management, antivenom if available, and supportive care like intubation, IV fluids and medication for symptoms. Outcomes are generally good with treatment.
Aluminum phosphide poisoning is a common lethal poisoning that has caused many deaths in India in recent decades. Aluminum phosphide is marketed as pesticide pellets or tablets used to preserve food grains. When it reacts with water, it produces phosphine gas which is toxic as it inhibits cellular respiration. Symptoms include vomiting, hypotension, and organ damage. Diagnosis involves detecting phosphine in breath or vomit. Treatment focuses on managing shock through fluids and vasopressors, as there is no antidote. Prevention requires better regulation of aluminum phosphide distribution to limit accidental or intentional ingestion.
This document provides guidance on snake bites in India. It discusses that snake bites are a major public health issue, killing over 11,000 people annually. The most common venomous snakes in India are Russell's viper, hump-nosed viper, cobra, and krait. It outlines the clinical presentation of envenomings from different snakes, including neuroparalytic effects from cobras and kraits, bleeding disorders from vipers, and muscle damage from sea snakes. The document emphasizes rapid assessment, resuscitation, detailed examination to identify the snake species, laboratory tests, and antivenom treatment for snake bites in India.
Snake Bite,Rabies,Scorpion Bite PPT – Presented By Prof.Dr.R.R.deshpande on 1...rajendra deshpande
This document provides information on snake bite treatment from an expert, Prof. Dr. R.R. Deshpande. It discusses the types of poisonous snakes in India and their distinctive features. It describes symptoms of neurotoxic and haemotoxic snake bites. The recommended treatment involves immobilizing the bitten area, applying ice or tourniquet, monitoring for symptoms, and administering Antivenom Serum intravenously if symptoms appear. It provides dosage guidelines and monitoring advice for different clinical presentations. Hospital admission is recommended for patients with bleeding, black urine or extensive paralysis.
The document outlines guidelines for the treatment of snake bites, including rapidly assessing the airway, breathing, and circulation of the patient; administering tetanus toxoid, antibiotics, and anti-venom as needed based on symptoms; and closely monitoring vital signs and administering supportive care for any complications like respiratory failure, hypotension, or renal failure. Laboratory tests are also recommended to identify coagulopathies or tissue damage from the venom.
This document summarizes the pathophysiology, clinical presentation, management, and prognosis of kerosene poisoning in children. Kerosene easily penetrates the lungs due to its low viscosity and surface tension, causing chemical pneumonitis that can lead to respiratory failure. Symptoms include cough, tachypnea, hypoxemia, and neurological effects. Management involves supportive care, with intubation and ventilation for severe cases. Gastric decontamination methods are not recommended due to risk of aspiration. Outcomes are generally good with supportive care alone, though extracorporeal membrane oxygenation or high frequency ventilation may be life-saving in severe cases unresponsive to conventional ventilation.
Bee and wasp stings can cause local reactions like pain, swelling and itching at the sting site. For some people, multiple stings or an allergic reaction can be life-threatening. Honeybees leave their stinger behind when they sting while wasps have a smooth stinger that can sting repeatedly. Symptoms of a local reaction include pain, swelling and hives near the sting that last up to a week. Removing the stinger, applying ice, taking antihistamines and pain relievers can help treat reactions. Wearing protective clothing and avoiding startling insects can help prevent stings.
This document provides information on corrosive poisoning, including types, mechanisms of injury, clinical features, investigations, management, and treatment. It discusses three main types of corrosives - acids, alkalis, and oxidants. Alkalis are noted as the most dangerous due to their ability to rapidly cause liquifactive necrosis and injury. Clinical features involve the gastrointestinal tract, respiratory system, eyes and skin. Investigations include endoscopy, imaging, and labs. Management focuses on airway protection, dilution, antibiotics, and monitoring. Long term complications like stricture formation may require repeated dilations.
This document provides an overview of immunity, including the basic concepts of innate and acquired immunity. It discusses the types of innate immunity like species, racial, and individual immunity. The mechanisms of innate immunity include anatomical barriers, phagocytic barriers, blood proteins, cytokines, inflammation, and fever. Acquired immunity is specific and involves the generation of immunological memory. It can be active, developed from natural infection or artificial vaccination, or passive, developed from maternal antibodies or antibody administration. The document also covers factors affecting individual innate immunity like age, hormones, and nutrition.
This document provides an outline and overview of ocular immunology. It begins with a definition of immunology and outlines the innate and adaptive immune systems. It describes components of the immune system including cells, tissues, and soluble factors. It then discusses ocular immunology, focusing on the immune response and privilege of different ocular tissues. Specific topics covered include uveitis immunology, hypersensitivity reactions, and selected autoimmune diseases involving the eye.
Effect of sub lethal doses of insecticides on natural enemies of crop pestsSAURABHPADAMSHALI
The document discusses natural enemies and the sublethal effects of insecticides on them. It begins by defining key terms like sublethal dose, insecticide, natural enemy, pest, integrated pest management, chemical control and biological control. It then provides tables listing important natural enemies and parasitoids of crop pests. The document discusses the physiological and behavioral effects insecticides can have at sublethal doses, including effects on development, fertility, mobility and orientation. It explains the mechanisms of insecticide penetration in insect bodies and their modes of action.
This document provides an overview of medical mycology, including:
1. It classifies fungi and discusses their morphology, including yeasts, molds, and dimorphic fungi.
2. It covers the epidemiology of fungal infections, including endemic mycoses.
3. It discusses the diagnosis of fungal infections through microscopy, culture, histology and other methods.
4. It outlines the treatment of fungal infections through antifungal drugs like amphotericin B and azoles.
This document provides an overview of medical mycology, including:
1. It classifies fungi and discusses their morphology, including yeasts, hyphae, and dimorphic fungi.
2. It covers the epidemiology of fungal infections, including endemic mycoses caused by dimorphic fungi.
3. It discusses the diagnosis of fungal infections through wet mounts, skin tests, serology, biopsy, and culture.
4. It outlines the treatment of fungal infections through antifungal agents like polyenes, azoles, and others.
Chemotherapy uses powerful chemicals to kill fast-growing cancer cells. It works by targeting cells that multiply quickly. Aminoglycosides are a class of antibiotics that kill bacteria by interfering with protein production. They have side effects like hearing loss and kidney damage. Inflammation is mediated by cellular and plasma factors called biomediators that increase vascular permeability. These include histamine, cytokines, and components of the complement system. Allergy is an immune response triggered by IgE antibodies binding to an allergen and activating mast cells. This causes symptoms through the release of inflammatory mediators.
Non-specific host defenses provide three lines of protection against invading pathogens:
1. Anatomical barriers like the skin and mucous membranes form the first line of defense.
2. Innate immune responses like inflammation, fever, and phagocytosis from white blood cells are the second line of defense.
3. The third line of defense involves specific immune responses from antibodies and lymphocytes that target pathogens that breach the first two lines. Together, these non-specific defenses provide broad protection against microbes.
Malaria and Antimalarial Drugs and recent Advancement Akhil Nagar
The document discusses advances in anti-malarial therapy. It describes the life cycle and pathogenesis of the malaria parasite Plasmodium. Several classes of antimalarial drugs are discussed, including quinine derivatives like chloroquine, aminoquinolines, antifolates like pyrimethamine, and artemisinin derivatives. Screening methods for antimalarial compounds include in vitro tests measuring parasite growth inhibition using radiolabeled hypoxanthine or microscopy. Drug combinations can help address drug resistance.
This document discusses insects of medical importance. It describes how some insects directly damage human tissue or act as vectors for disease. It also notes that insects provide useful medical services like producing drugs and serving as models for genetic studies. It focuses on stinging insects, describing their venom and allergic reactions. It discusses malaria transmission by mosquitoes and trypanosomiasis spread by tsetse flies. The document also outlines pharmaceutically important uses of insect venom, maggot therapy, and genetic model organisms like fruit flies.
This document discusses various types of fungi and fungal infections. It begins by describing the characteristics of fungi, including their cell structure and cell wall composition. It then classifies fungi and describes the types of infections they can cause, including superficial, subcutaneous, systemic, and opportunistic infections. Specific fungal genera and species that cause different types of infections are identified. The document also categorizes and describes various classes of antifungal drugs, including their mechanisms of action, pharmacokinetics, therapeutic uses, and adverse effects. These drug classes include azoles, polyenes like amphotericin B, and echinocandins.
1. The study assessed the efficacy of Nitazoxanide, Myrrh Total Oil, and Mirazid in treating Schistosomiasis mansoni infections in mice compared to Praziquantel.
2. Mice were infected with S. mansoni cercariae and treated with the drugs 50 days later. Efficacy was evaluated by examining parasite burden reduction, egg counts in stool and tissues, and biochemical changes.
3. Praziquantel showed the highest reduction in worm burden (up to 97%) and egg counts. Nitazoxanide and Mirazid also significantly reduced parasite burden and egg counts, though not as strongly as Praziquant
This document discusses different types of light and electron microscopes used to view microorganisms. It describes brightfield, darkfield, phase-contrast, and fluorescence microscopes under the light microscope section. It then discusses transmission electron microscopes and scanning electron microscopes. Key differences between TEM and SEM are provided.
Host pathogen interactions - This presentation is about the Host pathogen interaction played between bacteria virus and the human body and it also explains about the different protein and enzymes secreted by pathogens to cause infection and diseases in human like the release of endotoxin and exotoxin.
ANTI FUNGAL DRUGS AFFECTING CELL MEMBRANE AND CELL WALL.Mohammad Bilal
The document discusses anti-fungal drugs that target the fungal cell membrane and cell wall biosynthesis. It provides an overview of fungal cell structure, focusing on the cell wall components of chitin and beta-glucans. It explains how drugs like echinocandins inhibit beta-glucan synthase to disrupt cell wall formation, and how azoles and terbinafine inhibit ergosterol biosynthesis in the cell membrane. The summary highlights the main cellular targets of anti-fungal drugs and how inhibiting key processes like chitin, beta-glucan, and ergosterol synthesis impacts fungal growth and viability.
Shigella is a Gram-negative bacterium that causes the infectious disease shigellosis or bacillary dysentery. It is transmitted via the fecal-oral route and infects the colonic epithelium. Clinical symptoms include abdominal cramps, bloody mucus in the stool, and fever. S. dysenteriae produces a Shiga toxin that can damage kidney and brain cells. The bacterium uses a type III secretion system and effector proteins to invade intestinal cells, spread intracellularly, and induce inflammation. Treatment involves rehydration and antibiotics such as ciprofloxacin. Prevention relies on hand washing and water sanitation.
This document discusses insects of medical importance. It describes how some insects directly damage human tissue or act as vectors for disease. Additionally, it explains how some insects provide useful medical services through substances like venom or maggots used for debridement. Key disease vectors discussed are mosquitoes transmitting malaria, fleas transmitting plague, and kissing bugs transmitting Chagas disease. The model organism Drosophila melanogaster is also described as useful for genetic studies.
This document discusses insects of medical importance. It describes how some insects directly damage human tissue or act as vectors for disease. Additionally, it explains how some insects provide useful medical services and are model organisms for genetic studies. Specific examples of stinging insects that cause allergic reactions are described. The life cycles and transmission of malaria, trypanosomiasis, and Chagas disease by insect vectors are summarized. The document also outlines how venom and other insect products are used for testing allergies, treating inflammation and wounds, and in traditional medicines. It concludes by noting that insects like fruit flies and mosquitos are important genetic model organisms.
The immune system document defines key terminology related to symbiosis, disease, and immunology. It then describes the different types of immunity, including nonspecific and specific responses. As part of the nonspecific response, it details the immune system's physical and mechanical barriers, as well as chemical factors like lysozymes and complement proteins. It also explains the roles of phagocytes such as neutrophils, monocytes, and macrophages in phagocytosis and the inflammatory response.
INFLAMMATION-A DISCUSSION OF VARIOUS ANIMAL MODELS FOR THE STUDY OF ANTI-INF...AishaKhan276
This slide includes;
1. Definition, causes and signs of Inflammation
2. Comparison between acute and chronic inflammation
3. Various animal models for Pre clinical testing of Anti-inflammatory agents
The different animal models are;
I. Vascular permeability
II. UV-erythema in guinea pigs
III. Croton-oil ear edema in rats and mice
IV. Paw edema in rats
V. Collagen Induced Arthritis
VI. Adjuvant Induced Arthritis
VII. Oxazolone-induced ear edema in mice
VII. Pleurisy tests
VIII. Granuloma pouch technique (various modifications and various irritants:
A. Cotton wool granuloma
B. Glass rod granuloma
IX. Papaya Latex Induced Arthritis
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Wasp sting: Inflammation & Relief by Topical Natural Phytochemicals
1. WASP STING:
INFLAMMATION & RELIEF BY TOPICAL
NATURAL PHYTOCHEMICALS
By
Kevin KF Ng, MD, PhD
Former Associate Professor of Medicine
Division of Clinical Pharmacology
University of Miami, FL, USA
Email: kevinng68@gmail.com
Presented at HealthCare provider Seminar 2018
2. Lecture Outline
▪ Introduction: what is a wasp? What is wasp sting?
▪ Statistics: incidence of wasp stings and deaths
▪ Composition of wasp venom
▪ Reactions to wasp sting by immune system
▪ Acute inflammation: mediators
▪ Treatment of wasp stings: Local reaction
Systemic reaction or anaphylaxis
▪ Anti-inflammatory phytochemicals
▪ Effect of topical anti-inflammatory phytochemicals
▪ Summary and future directions.
2
3. Modified taxonomic tree of stinging insects
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4517515/
waswasp
Vespula
3
4. Background of Wasp Stings
▪ More than 25,000 species of wasps exist worldwide. They include yellow jackets,
paper wasps and hornets of the genus Vespula.
▪ Stings by members of the order Hymenoptera is a major cause of morbidity and
mortality and accounts for more fatalities than any other venomous animal.
▪ The skin is the most commonly affected organ system.
▪ The result of a wasp sting can vary from a single area of localized inflammation
to a generalized urticarial rash.
4
5. Hymenoptera (wasp, hornets, yellow jackets) Stings in USA
▪ Annual number of stings by Hymenoptera
• more than1 million stings annually
• 3% of general population
▪ Systemic reactions
• 0.4-0.8 % of children
• 3 % of adults
https://emedicine.medscape.com/article/768764-overview#a6
Deaths from 2003-2010
5
6. Incidence of Reactions to Wasp Stings in Emergency Room
n = 2606 patients in 2015
https://emedicine.medscape.com/article/169324-overview#a6
Under
reported
Hymenoptera stings and wasp stings
have increased over the last 60 years
and now account for over 79 deaths
each year.
6
7. Fatal occupational injuries involving insects 2003-2013
http://blogs.marketwatch.com/capitolreport/2014/08/11/bee-stings-are-behind-most-insect-related-deaths-for-workers/
number
7
9. Features of an adult female worker wasp
▪ Length: 0.5-1.0 inch
▪ Stinger: 2.5 mm/0.1 inch
▪ Narrow waist
▪ Black and yellow stripes in abdomen
▪ Only worker wasp (sterile female) has stinger
▪ Fly with legs hanging down
▪ Feed on flower nectar, ripen fruits and insects https://www.confirmakill.co.uk/services/wasp-nest-removal/
stinger 2.5 mm
0.5-1.0 inch
9
10. Wasp sting and the stinger apparatus
http://www.vapaguide.info/page/23https://www.healthline.com/health/wasp-sting
10
11. Only female (sterile) adult worker wasp stings
▪ Only female worker wasp has stinger
▪ It stings when threatened
▪ It does not pollinate
▪ It does not make honey
▪ It feeds on other insects and human food
▪ It’s average life span is 12-22 days
▪ The queen has an average lifespan of 12 months
http://www.stepin2mygreenworld.com/healthyliving/around-the-home/wasp-repellent/
https://www.landcareresearch.co.nz/science/plants-animals-fungi/animals/.../wasps/faq
11
12. Which insect gives the most painful sting?
Adapted from www.bbc.com/earth/story/20150312-the-worlds-most-painful-insect-sting
12
13. Reactions to a wasp sting
▪ A local reaction: Pain, swelling and redness lasting 30
minutes to 3 hours
▪ A large local reaction causes swelling beyond the sting
site. The large local reactions peak at about 48 hours
and last 5 to10 days
▪ The most serous reaction is an allergic response to the
sting. It needs to be treated immediately with Epi-pen
and followed up at Emergency Room
13
14. What happens when a wasp stings ?
(1 sting contains 2 – 15 µg venom)
Acute Inflammation
to 10 days
to 5 days
14
27. Pathophysiology (local reaction)
▪ Wasp does not leave the stinger behind.
▪ Wasp venom contains up to 13 different antigens.
▪ The venom causes an intense stinging sensation that is mediated by acetylcholine
and serotonin, which make up to 5% of the dry weight of the venom
▪ The acetylcholine stimulates nociceptors within the dermis. The serotonin causes
multiple effects through the 5-hydroxytryptamine (5-HT) receptors,
▪ The wasp venom also contains phospholipase A2 , phospholipase B, as well as
mastoparan peptide, which can cause direct mast cell degranulation with the release
of histamine.
https://emedicine.medscape.com/article/169324-overview#a5 27
28. Pathophysiology (Systemic reaction)
▪ For individuals who have been sensitized to the venom by a past exposure to
Hymenoptera venom, symptoms may progress
▪ This progression involves elements of both cellular and humoral immunity.
▪ The cellular components consist of lymphocytes, both T cells (CD4+ and CD8+) and B
cells, macrophages, and mast cells
▪ The humoral factors include immunoglobulin E (IgE) and cytokines.
▪ An immediate type of hypersensitivity occurs when the sensitized mast cells contact the
offending allergen.
▪ This is followed by degranulation with the release of chemical mediators: histamine,
heparin and tumor necrosis factor (TNF).
▪ Mast cell activation also initiates generation of bioactive products through lipid
metabolism of arachidonic acid and the production of cytokines such as TNF, interleukin
(IL)–6, IL-4, and IL-5.
▪ Mast cells are located in the connective tissue of the dermis, intestinal tract, airway, and
lungs and around the vascular system, thus producing the symptoms of urticaria,
vomiting, abdominal cramp, diarrhea, dyspnea and wheezing.
https://emedicine.medscape.com/article/169324-overview#a5
28
29. Signs and symptoms of Severe Wasp Sting or
Anaphylaxis
https://en.wikipedia.org/wiki/Anaphylaxis
Treatment
● Epi-Pen
● Call 911 or
go to Emergency Room
29
30. Pathophysiology: severe systemic or anaphylactic reaction
▪ Anaphylactic shock is an immediate-type hypersensitivity reaction that occurs when
mast cells are activated within multiple organ systems.
▪ The explosive of histamine and cytokines cause vascular collapse.
▪ This is an IgE-mediated reaction to the sting.
▪ Most fatalities occur within 1 hour, with most severe reactions occurring within 10
minutes of the wasp sting.
▪ In one study of postmortem sera from patients with sudden death, 23% had
elevated levels to Hymenoptera venom
https://emedicine.medscape.com/article/169324-overview#a5
30
31. Requirements for rational treatment of
local reaction to wasp sting
▪ Knowledge of the severity of reaction to wasp sting
▪ Knowledge of potency of wasp venom
▪ Knowledge of components of wasp venom
▪ Knowledge of the immune response to wasp venom
▪ Knowledge of the natural history of wasp sting
▪ Knowledge of pharmacology of anti-inflammatory drugs and phytochemicals.
▪ Combination of these knowledges help to formulate a natural topical product based
on anti-inflammatory phytochemicals,
31
32. Potency of Hymenopteran Venom
(1/lethal dose x 1000)
Source: Schmidt, J.O. (1983) Hymenoptera envenomation
Potency
32
33. Lethality of Hymenopteran Stings
http://www.artasaweapon.info/pages/bullet-ant-sting-pain-scale.awp
33
40. Remedies for Local Reaction to Wasp Sting
Home remedy
▪ Ice
▪ Garlic
▪ Onion
▪ Cucumber
▪ Lemon
▪ Baking soda ?
▪ Honey
OTC product
▪ Antihistamine cream
▪ Hydrocortisone cream
▪ Ibuprofen
40
41. Disadvantages of Current Topical Renedies
▪ Low concentration of active ingredients.
▪ Water-based formulations.
▪ Inefficient and slow absorption.
▪ Actions limited to 2 out of 14 mediators.
▪ Many mediators act unopposed.
▪ Therefore treatment is incomplete.
▪ This prompted us to search for anti-inflammatory
phytochemicals among food, spices and herbs.
41
42. What are phytochemicals?
▪ Phytochemicals are bioactive substances
found in plants.
▪ Phytochemicals are found in roots, barks, stems,
leaves, flowers, fruits.
▪ Phytochemicals are beneficial for health.
▪ Phytochemicals are not vitamins.
▪ Phytochemicals can be extracted by water,
alcohol or massage oils.
42
43. Bioactive functions of phytochemicals
Anti-inflammatory
Anti-oxidant
Anti-aging
Anti-cancer
43
44. Examples of anti-inflammatory phytochemicals
in food, spices and herbs
Product Variety Active ingredient
Fruit Grape Resveratrol
Apple Quercetin
Chili pepper Capsaicin
Leaf Green tea EGCG
Aloe Salicylic acid, emodin
Bark White willow Salicylic acid
Magnolia Honokiol
Root Ginseng Ginsenosides
Galagal Galagin
Tumeric Cucumin
44
47. 15 Molecular targets of synthetic drugs and phytochemicals
Food
Spices
Herbs
Synthetic
drugs
47
48. A phytochemical formula L35 made from spices and herbs for
Itch, Pain and Swelling caused by wasp sting
chemical mediators
Spices
Herbs
48
49. Preparation of extracts of phytochemicals with
alcohol and olive oil
Botanical materials + extraction solvent
Temperature controlled treatment
Homogenization
Differential centrifugation
Residue removal
Recovery of filtrate
49
50. Effects of extracts of spices or herbs on
itch & swelling caused by wasp sting
▪ Oil extract with one anti-inflammatory herb: no relief
▪ Oil extract with two anti-inflammatory herbs: slight relief
▪ Oil extract with three anti-inflammatory herbs: moderate relief
▪ Oil extract with four or more anti-inflammatory herbs : significant relief
50
51. Effect of L35 on symptoms of wasp sting:
significant relief
Pain
Redness
Swelling
Itch
● Control data based on family exposure
● L35 Rx based on personal experience
51
52. Summary
▪ Only female (sterile) adult worker wasp stings.
▪ Wasp venom contains allergens, proteins, peptides, enzymes, etc.
▪ Exposure of the skin to wasp venoms results in the release of multiple
chemical mediators which cause a local reaction, severe systemic
reaction or anaphylaxis.
▪ Local reactions are self-limiting, whereas severe systemic and toxic
reaction require immediate medical intervention.
▪ Home remedies and OTC products for local reactions are generally
not effective as they target only a few chemical mediators.
▪ A combination of phytochemicals target most of the chemical
mediators and provide a rational local treatment of wasp stings.
52