Vicam Ia Working Principle
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This document summarizes several antibody-based affinity columns and tests for detecting various mycotoxins. It describes the basic process by which monoclonal antibodies are used to selectively bind mycotoxins from samples, which can then be purified and detected. It then provides details on the detection limits, ranges, precision and validation of various test kits for aflatoxins, fumonisins, zearalenone, ochratoxin A, deoxynivalenol, T-2 toxin and others. All tests utilize monoclonal antibody columns and affinity techniques for selective extraction and quantification of different mycotoxins from parts per billion to parts per million levels.
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Vicam Ia Working Principle
This document describes several mycotoxin affinity columns and test kits produced by VICAM. The columns work by binding monoclonal antibodies to mycotoxins like aflatoxins, fumonisins, zearalenone, ochratoxin A, deoxynivalenol, and T-2 toxin. Sample extracts are passed through the columns, which capture the bound mycotoxins while impurities pass through. Elution with methanol releases purified mycotoxins. The test kits use the columns coupled with fluorometry or HPLC to detect various mycotoxins at low ppb to ppm levels with good precision and accuracy. The kits have applications in food safety testing.
Vicam Ia Technology Working Principle
1. The document compares the Vicam immunoaffinity column (IA) method to ELISA for mycotoxin testing.
2. The Vicam IA method is faster, easier to use, more convenient for storage and ordering supplies, and allows for lower limits of detection than ELISA.
3. Specifically, the Vicam IA method takes less than 15 minutes to run per sample while ELISA takes 20 minutes or more, and the Vicam IA method can detect mycotoxins at levels as low as 0.5 ppb while ELISA detection limits are 1-5 ppb.
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This document provides details on procedures and acceptance criteria for qualifying various components of an HPLC instrument, including:
1. The solvent delivery system is tested for flow rate accuracy at different flow rates and gradients, with acceptance criteria of ±5.0% for isocratic and ±2.0% for gradient methods.
2. The injector is tested for volume precision and carryover, with limits of RSD ≤ 1.0% and carryover ≤ 0.2%.
3. The detector is tested for linearity using caffeine standards, with an r2 acceptance criterion of ≥0.99900, and for wavelength accuracy across caffeine's maxima and minima, with a limit of ±2nm
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Vicam Ia Working Principle
This document describes several mycotoxin affinity columns and test kits produced by VICAM. The columns work by binding monoclonal antibodies to mycotoxins like aflatoxins, fumonisins, zearalenone, ochratoxin A, deoxynivalenol, and T-2 toxin. Sample extracts are passed through the columns, which capture the bound mycotoxins while impurities pass through. Elution with methanol releases purified mycotoxins. The test kits use the columns coupled with fluorometry or HPLC to detect various mycotoxins at low ppb to ppm levels with good precision and accuracy. The kits have applications in food safety testing.
Vicam Ia Technology Working Principle
1. The document compares the Vicam immunoaffinity column (IA) method to ELISA for mycotoxin testing.
2. The Vicam IA method is faster, easier to use, more convenient for storage and ordering supplies, and allows for lower limits of detection than ELISA.
3. Specifically, the Vicam IA method takes less than 15 minutes to run per sample while ELISA takes 20 minutes or more, and the Vicam IA method can detect mycotoxins at levels as low as 0.5 ppb while ELISA detection limits are 1-5 ppb.
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This document discusses high performance thin layer chromatography (HPTLC), comparing it to traditional thin layer chromatography (TLC). HPTLC involves the same principles as TLC but with some key improvements like smaller particle sizes, faster separations, and the ability to perform quantitative analysis using scanning densitometry. The document outlines the basic steps of an HPTLC procedure including sample preparation, selecting the chromatographic layer, pre-washing and pre-conditioning the layer, applying the sample, developing the plate, detecting spots, scanning, and documentation. It also discusses factors that affect the separation like the mobile phase composition and saturated vapor. HPTLC is described as having applications in pharmaceutical analysis, cosmetics analysis, and
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1. The solvent delivery system is tested for flow rate accuracy at different flow rates and gradients, with acceptance criteria of ±5.0% for isocratic and ±2.0% for gradient methods.
2. The injector is tested for volume precision and carryover, with limits of RSD ≤ 1.0% and carryover ≤ 0.2%.
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2. Analytical methods for mycotoxins include confirmatory multi-analyte LC-MS/MS, which can measure over 25 mycotoxins simultaneously, and untargeted high resolution MS for identifying unknowns.
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Mycotoxins are toxic compounds produced by molds that can contaminate food and cause illness in humans and animals. Over 350 mycotoxins have been identified. The term was first used in 1961 after an outbreak in England linked to contaminated peanut meal killed thousands of animals. Mycotoxins act as a chemical defense for molds, protecting them from predators. Major mycotoxins include aflatoxins, tricothecenes, and ochratoxins. Exposure can occur through ingestion, inhalation, or skin contact. Health effects range from acute poisoning to cancer. Intervention focuses on preventing mold growth in crops and buildings to reduce exposure.
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Mycotoxins
Mycotoxins are toxic metabolites produced by molds that can contaminate livestock feeds. While the rumen can break down some mycotoxins, the capacity decreases with high-concentrate diets. Mycotoxins can cause reduced performance, diarrhea, and increased disease incidence in stressed animals like fresh cows. Symptoms are often nonspecific but can impact the immune system and antioxidant levels subclinically. Both acute and chronic low-level mycotoxin exposure present economic risks, making prevention and treatment important.
Mycotoxin
Mycotoxins are toxic compounds produced by fungi that can contaminate food and cause illness in humans and animals. The term was first used in 1961 after an outbreak killed thousands of animals that had eaten contaminated peanut meal. Mycotoxins are secondary metabolites of fungi that act as a chemical defense system. Major mycotoxins include aflatoxins, fumonisins, ochratoxins, patulin, and trichothecenes which are produced by fungi such as Aspergillus, Fusarium, and Penicillium. Mycotoxin poisoning has no cure and can cause death, organ damage, cancer, and birth defects. Prevention through monitoring and controlling fungal growth is important
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The document discusses strategies for controlling mycotoxins in animal feed, including: co-contamination of multiple mycotoxins increases toxicity; proper sampling is needed to diagnose mycotoxin issues; prevention strategies during cultivation and storage can reduce mycotoxin formation; and mycotoxin adsorbents or binders can be used to prevent absorption of mycotoxins through physical or chemical binding in the gastrointestinal tract.
Mycotoxin in poultry feeds ppt
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Mycotoxins
Dr. Babasaheb Nagurao Kumbhar discusses mycotoxins, which are toxic substances produced by fungi that grow on crops. Some key points:
- Mycotoxins are secondary metabolites of fungi that are toxic to other organisms. Unlike bacterial toxins, they are not usually detectable by immune systems.
- Over 300 mycotoxins are known. Major ones of concern include aflatoxins, deoxynivalenol, zearalenone, fumonisin, T-2 toxin, and ochratoxin A.
- Factors like temperature, moisture levels, and storage conditions can favor mycotoxin production. Common toxigenic fungi
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This document summarizes ASTM standard test method D6591-19 for determining aromatic hydrocarbons in middle distillates using HPLC-RID. It involves using an HPLC with a refractive index detector to separate and quantify different types of aromatic hydrocarbons. The method specifies using a polar stationary phase column, heptane mobile phase, and particular aromatic hydrocarbon reference standards. It also provides the calculation to determine the mass percent of different aromatic hydrocarbons based on peak area responses from calibration curves.
Food contaminants dhaka university_august2014
This document discusses food contaminants and methods for their analysis. It defines contaminants as unintended substances in food, including naturally occurring toxins, pesticide and drug residues, heavy metals, and chemical hazards from food processing. Maximum residue levels (MRLs) set legal limits for contaminants to protect consumers and ensure fair trade. Analysis methods include fast screening tests and more sophisticated confirmatory methods using techniques like LC-MS/MS and GC-MS/MS. Method validation establishes reliability through parameters like selectivity, accuracy, precision and reproducibility. The document presents examples of validated multi-residue methods for organochlorine pesticides in fish and formaldehyde in foods. Overall it aims to improve food safety in Bangladesh through a
FTIR For Stack and CEM
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Analytical Method Validation
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Clora-Online
The CLORA On-Line Analyzer uses monochromatic wavelength dispersive X-ray fluorescence (MWD XRF) to continuously analyze total chlorine concentrations between 0.2-3000 ppm in crude oil, water, and process streams. It provides real-time, precise measurements without requiring consumables. The analyzer operates on a variety of sample types and viscosities up to 160 cSt at pressures up to 80 psi and temperatures up to 300°F. It offers communication options and high precision, as demonstrated by its ability to repeatedly measure chlorine in crude oil and water samples with standard deviations of 0.4 ppm or less over 5-minute runs.
Qualification of high performance liquid chromatography
Qualification of high performance liquid chromatographysrikrupa institute of pharmaceutical analysis
The document provides an overview of the qualification process for high performance liquid chromatography (HPLC) equipment, including design qualification (DQ), installation qualification (IQ), operational qualification (OQ), and performance qualification (PQ). It describes the objectives and procedures for each qualification step. Key aspects covered include verifying design specifications, proper installation, operational requirements such as precision, accuracy and noise levels, and ongoing performance monitoring. The goal of qualification is to ensure analytical systems are suitable for their intended use and generate reliable results.Pharmaceutical validation
The document discusses the qualification of analytical equipment. It describes the types of qualification which include design qualification, installation qualification, operational qualification, and performance qualification. It provides details on qualifying various analytical instruments such as FTIR, GC, and HPLC. Specific parameters to be tested during qualification are discussed for components like the inlet, oven, detector, autosampler, and others, along with typical acceptance criteria. The document emphasizes the importance of qualifying analytical equipment to ensure it is properly installed, operates correctly, and provides expected results.
validation of analytical method used in cleaning
The document discusses validation of analytical methods used in cleaning validation. It covers parameters assessed in analytical method validation like specificity, linearity, range, accuracy, precision, LOD, LOQ. It also discusses method validation, cleaning validation, levels of cleaning, cleaning process validation, typical analytical procedures and their applicability. Key aspects of validation covered include equipment and personnel qualification, microbiological considerations, documentation, sampling, rinsing, rinse samples, detergents used and establishment of acceptable limits.
Analytical method validation
This document provides an overview of analytical method validation. It discusses key validation characteristics such as specificity, linearity, range, accuracy, precision, LOD and LOQ. Guidelines for validation from organizations like ICH, USP, ANVISA and AOAC are also mentioned. The document describes procedures for establishing various validation parameters and evaluating the results. It emphasizes that validation is necessary to ensure analytical methods consistently provide reliable results.
CLEANING VALIDATION for M.pharm and industry person
YOU CAN EASY WAY TO UNDERSTAND A PROCESS AND ANLYTICAL METHOD OF CLEANING VALIDATION
Cleaning validation is the methodology used to assure that a cleaning process removes residues of the active pharmaceutical ingredients of the product manufactured in a piece of equipment, the cleaning aids utilized in the cleaning process and the microbial attributes.[1] All residues are removed to predetermined levels to ensure the quality of the next product manufactured is not compromised by waste from the previous product and the quality of future products using the equipment, to prevent cross-contamination and as a GMP requirement.
The U.S. Food and Drug Administration (FDA) has strict regulation about the cleaning validation. For example, FDA requires firms to have written general procedures on how cleaning processes will be validated. Also, FDA expects the general validation procedures to address who is responsible for performing and approving the validation study, the acceptance criteria, and when revalidation will be required. FDA also require firms to conduct the validation studies in accordance with the protocols and to document the results of studies.The valuation of cleaning validation is also regulated strictly, which usually mainly covers the aspects of equipment design,cleaning process written, analytical methods and sampling. Each of these processes has their related strict rules and requirements. Regarding to the establishment of limits, FDA does not intend to set acceptance specifications or methods for determining whether a cleaning process is validated. But some limits that have been mentioned by industry include analytical detection levels such as 10 PPM, biological activity levels such as 1/1000 of the normal therapeutic dose and organoleptic levels.[2][3][4]
Cleaning Validation in the context of Active Pharmaceutical Ingredient manufacture may be defined as: "The process of providing documented evidence that the cleaning methods employed within a facility consistently controls potential carryover of product (including intermediates and impurities), cleaning agents and extraneous material into subsequent product to a level which is below predetermined levels".
validation and calibration of HPLC
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Vicam Ia Working Principle
- 1. Antibody Bound Resin Mycotoxin Affinity Column
- 5. Impurities Mycotoxins bound to monoclonal antibodies
- 6. Column Wash
- 7. Mycotoxins bound to monoclonal antibodies
- 10. AflaTest • Limit of detection as low as 0.5 ppb (fluorometer) 0.05 ppb (HPLC) • Wide range as high as 320 ppb • AOAC Official Method status • USDA-FGIS Certificate of Conformance
- 11. FumoniTest • 0.25 ppm (fluorometer) 0.016 ppm (HPLC) limit of detection • Wide range of 0-10 ppm • Excellent linearity: r = 0.998 • Excellent precision: %CV < 11% from 1-10 ppm • Fluorometer method correlates well with HPLC linear regression r = 0.995 • USDA-FGIS Certificate of Conformance
- 12. ZearalaTest • 0.10 ppm (fluorometer) 0.0025 ppm (HPLC) limit of detection • Wide range of 0-5 ppm • Excellent linearity r = 0.999 • Excellent precision: %CV = 13% over the assay range • Fluorometer method correlates well with HPLC linear regression r = 0.990
- 13. OchraTest • Limit of detection as low as 1 ppb (fluorometer) 0.25 ppb (HPLC) • Wide range as high as 100 ppb • Excellent linearity with correlation coefficient (r) of 0.997-0.999 for wheat, dried fruit and green coffee • Excellent precision with %CV <10% at 20 ppb • Fluorometer method correlates well with HPLC when analyzed by linear regression
- 14. DON FQ Quick - Results in less than 30 minutes (after sample extraction. Sensitive - Detects levels as low as 0.5 ppm Wide Range - Measures up to 5 ppm Convenient - Uses same instrumentation as other VICAM mycotoxin tests USDA-FGIS Certificate of Conformance
- 15. DONtest HPLC • limit of detection : 0.1 ppm (HPLC) • Wide range : 0 -10 ppm • Excellent linearity with correlation coefficient (r) of 0.999 ： r2 = 0.999 • Excellent precision : with %CV 8.3% • DONtest-HPLC method correlates well with GC- ECD when analyzed by linear regression: r2 = 0.982
- 16. T-2 Quick - Results in less than 20 minutes (after sample extraction. Sensitive - Detects levels as low as 0.15 ppm Wide Range - Measures up to 2.0 ppm Convenient - Uses same instrumentation as other VICAM mycotoxin tests Safe - Requires less toxic materials than other methods.
- 17. T-2 HPLC • limit of detection : 0.005 ppm • Wide range : 0 –1.5 ppm • Excellent linearity with correlation coefficient (r) of 0.999 ： r2 = 0.999 • Excellent precision : RSD 3.68%
- 18. Team Medical & Scientific Sdn. Bhd. 41, Jalan Anggerik Vanilla T31/T, Kota Kemuning, 40460 Shah Alam, Selangor Tel : 603-5122 5108 Fax : 603-5122 1608 Email : info@tms-lab.com Blog : tms-lab.blogspot.com Solely Distribute by :