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• Comparison with Evaporative Light Scattering Detectors
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• How Charged Aerosol Technology Works
• Comparison with Evaporative Light Scattering Detectors
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• Examples of Applications
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This presentation describes the combined advantages of a reagent-free capillary format Ion Chromatography (IC) to determine monosaccharides and disaccharides in various applications, from low concentrations in synthetic urine samples to high concentrations in beverage samples. In a reagent-free IC system, the hydroxide eluent is electrolytically generated inline to deliver accurate and precise concentrations for isocratic or gradient separations by only adding deionized water. Eluent generation eliminates carbonate contamination and errors from manual preparation. A capillary scale system with µL/min flow rates can run 24/7, always on and always ready for samples.
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Pool side testing
1. 1
New Methodology for Poolside Compliance Testing
By Ivars Jaunakais
World Aquatic Health Conference
Friday, October 17, 2008 - 2:30 pm
www.sensafe.com / ivars@sensafe.com
2. 2
Water
• It’s the most important natural resource in
our environment
• Not pure - contains microorganisms and
dissolved minerals which need to be
controlled so water can be used safely
3. 3
Chlorine in Water
• Effective against a broad range of
microorganisms
• Chlorine first used in 1908 for public health
• More than 79,000 tons of chlorine are
used per year in the United States and
Canada to treat water
• Monitoring chlorine is very important
4. 4
Pool disinfection
• To protect people, water clarity and
equipment
• Several pathogens can be transmitted in
water
• Inactivation of pathogens depends on
contact time
• Public health requires pool water testing
for Hypochlorous acid or free chlorine
residual concentration
5. 5
Germ inactivation time in
1 ppm chlorinated water
GERM INACTIVATION TIME
E. Coli O157:H7
Bacterium
Less than 1 minute
Hepatitis A
Virus
About 16 minutes
Giardia
Parasite
About 45 minutes
Cryptosporidium
Parasite
About 9600 minutes
(6.7 days)
6. 6
Some sources of Chlorine
Chemical
Name
Chemical
Formula
Form % Chlorine
Chlorine
Gas
Cl2 Gas 100%
Calcium
Hypochlorite
Ca(OCl)2 Solid 65-70%
Sodium
Hypochlorite
NaOCl Liquid ~12%
7. 7
About the sources of Chlorine
• Despite their chemical and physical
differences they all form hypochlorous acid
• This change occurs when water is added
• Hypochlorous acid (HOCl) is the actual
disinfecting agent
8. 8
Hypochlorite ion
• The sum of Hypochlorous acid
and Hypochlorite ion is called free
chlorine, and the chemical
equation or relationship is:
9. 9
Hypochlorous acid reactions
• Two chemical reactions impact the performance of
Hypochlorous acid as a disinfectant:
• First reaction involves a hydroxide ion (OH¯)
• OH¯ is available in aqueous solution especially when pH
level is above 7 which causes Hypochlorous acid to form
Hypochlorite ion
• Hypochlorite ion is less than one third as effective a
disinfectant as Hypochlorous acid
• Graph 1 shows the relationship between pH versus
chlorine species (Hypochlorous acid and Hypochlorite
ion)
10. 10
pH versus chlorine speciesGraph 1
Percent HOCl and OCl-
vs. pH
0
10
20
30
40
50
60
70
80
90
100
6.0 6.5 7.0 7.5 8.0 8.5
pH
PercentHOClandOCl-
HOCl
OCl-
HOCl
Hypochlorous Acid
OCl-
Hypochlorite Ion
11. 11
Chlorine reaction
• Second chlorine reaction is with ammonia
(NH3) and organic nitrogen compounds such as
proteins and amino acids in the pool
• A series of reactions occur that form
chloramines
• Chloramines are less effective as a disinfectant
• Active chlorine can be transferred from inorganic
chloramine to amine (organic) containing
compounds
12. 12
Combined Chlorine Equations
• The sum of the chloramine species is
called combined chlorine, and chemically
created in the pool as follows:
13. 13
Breakpoint Chlorination
• The process which eliminates both the
combined chlorine and the ammonia
problem responsible for creating the
chloramine is called Breakpoint Chlorination
• In the pool industry its called “Shock”
14. 14
Total Chlorine
• Total chlorine is the sum of free chlorine
and combined chlorine
• Free chlorine and total chlorine are
monitored by automated equipment and
confirmed by poolside testing for swimmer
protection
Total Chlorine =
Free Chlorine + Combined Chlorine
15. 15
To achieve good test results
1. Test is acceptable or compliant
2. Test is appropriate for the staff technical
ability
3. Test is robust, reagents and
equipment are reliable & stable
4. Test is not affected by interferences
5. Test is accurate when staff performs the
test correctly
16. 16
US EPA accepted test methods
• Amperometric Method
• DPD-FAS Titrimetric Method
• DPD Colorimetric Method
• Syringaldazine (FACTS) Colorimetric
Method
• TMB (3,3’,5,5’Tetramethylbenzidine)
SenSafe™ Aperture Colorimetric Test
Strip Method
(Approved by the US EPA in 2007)
17. 17
DPD Chlorine testing
• DPD methods have become preferred for
chlorine measurement
• The DPD methods determine concentration from
the color formed when chlorine reacts with the
DPD
• DPD-FAS Titration method determines the
chlorine by measuring the amount of FAS Titrant
needed to bleach out the DPD-chlorine color
formed
• Most state health departments recommend or
accept DPD tests because they are quick, enjoy
wide acceptance and have US EPA approval
18. 18
DPD Chlorine Test
Manufacturers
• Hach Company
• LaMotte Company
• Taylor Technologies, Inc.
• HF Scientific, Inc.
• Palintest LTD
• Industrial Test Systems, Inc.
• Others
19. 19
DPD delivery methods
• DPD liquid reagents (2)
• DPD tablet
• DPD powder pillow
• eXact® Strip Micro DPD-1 and
DPD-3
(All meet 4500-CL G reportable method)
• DPD is used for colorimetric or
the DPD-FAS Titration methods
to determine the chlorine
concentration
20. 20
New DPD methodology for
poolside compliance testing
System consists of two
items:
1. Meter with sealed
sample cell
2. eXact® Strip Micro
DPD-1, DPD-3
(reagent delivery
device)
21. 21
New DPD methodology for
poolside compliance testing
Step 1
• Rinse the sample cell,
press the “ZERO/ON”
button, dip meter
upside down to elbow
length turn meter
upright to sample pool
water and remove
meter from the pool.
22. 22
New DPD methodology for
poolside compliance testing
Step 2
1. Press “ZERO/ON” button and
meter will zero and display 0.00.
23. 23
New DPD methodology for
poolside compliance testing
Step 3
1. Press “READ” button and
immediately dip eXact® Strip Micro
DPD-1 into water sample for the 20
second count down. During the 20
seconds constantly move the strip
back and forth, which releases the
DPD and buffer reagents from the
test strip while mixing the sample.
24. 24
New DPD methodology for
poolside compliance testing
Step 4
• At the end of the 20 seconds the
meter will display “1” at which
time you remove and discard the
strip and the meter automatically
reads and displays the free
chlorine concentration, and
stores the result in memory.
Free Chlorine = 1.58 ppm
25. 25
New DPD methodology for
poolside compliance testing
Step 5
• Continue the test to determine the Total
Chlorine concentration: Press “READ”
button and simultaneously dip eXact® Strip
Micro DPD-3 into the water sample for 20
second count down.
• During the 20 seconds constantly move the
strip back and forth, which releases the
Potassium Iodide reagent from the strip and
mixes the sample.
26. 26
New DPD methodology for
poolside compliance testing
Step 6
• At the end of the 20 seconds the meter will
display “1” at which time you remove and
discard the strip and the meter
automatically reads and displays the total
chlorine concentration, and stores the
result in memory.
• Discard sample and rinse with water before
storage. This DPD test method is compliant
for health department and US EPA
requirements.
Total Chlorine = 1.89 ppm
27. 27
Total chlorine (TC) =
free chlorine (FC) + combined chlorine
• Combined chlorine = TC - FC
• Free chlorine = 1.58 ppm (FC)
• Total chlorine = 1.89 ppm (TC)
• Combined chlorine = 1.89 – 1.58 = 0.31 ppm
• Combined chlorine is above the recommend level of 0.20
ppm and suggests pool needs to be shocked: in this
example ( 10 X 0.31 = 3.1 ) this pool can be shocked by
increasing the pool chlorine level by 3.1 ppm
28. 28
Benefits of this DPD
methodology
• No cell or test tube to handle
• Safe and easy to use DPD delivery device
• The eXact® DPD Strip is stable
• Non technical procedure
• Quick
• Accurate
29. 29
Chlorine Test Specifications
METER MENU RANGE (PPM) RESOLUTION
Micro 7+ CL1 0.00 – 2.39
2.4 – 10.8
0.01
0.1
DR890 Program 9 0.00 – 2.20 0.01
Program 12 0.0 – 11.0 0.1