The document compares the Vicam immunoaffinity column (IA) method to the ELISA method for analyzing AflaToxin. The Vicam IA method can be trained in an hour, takes less than 15 minutes to run a test, has products that can be stored at room temperature for 1.5 years, uses reusable standards, and displays results directly in ppb or ppm. The ELISA method is more difficult to understand, takes 20 minutes or more to run a test, requires refrigerated storage of products with a 6 month shelf life, requires separate purchase and use of standards for each batch, displays data as groups requiring separate calculation, and kits must be ordered with matched reagents. The Vicam

11. Elute AflaToxin
with 1.0 ml Methanol
Add Developer Solution Dilute with H2O
Measure in Fluorometer Inject into HPLC

18. Vicam Immunoaffinity Column
vs. ELISA

19. Training
Vicam IA: Can be trained in an hour.
Concept of method easy to understand.
ELISA: Operator must measure and mix
very small volumes, which can bead to
errors. Concept of method difficult to
understand.

20. Time to run test
Vicam IA: Less than 15 minutes.
ELISA: 20 minutes or more.
Test requires 2 incubations of 5 or 10
minutes.

21. Storage of products
Vicam IA: Room temperature and shelf life
up to 1.5 years from date of production.
ELISA: Must be refrigerated and 6 month
shelf life. Must throwing away expired
product.

22. Standards
Vicam IA: Reusable standards to calibrate
the fluorometer. One time low cost.
ELISA: Must run 3 to 5 standards with
every batch. Customer must pay to run
each standard, which is costly.

23. Data display
Vicam IA: Fluorometer printout in ppb or
ppm for each sample.
ELISA: Data printout in groups or must be
calculated by using a separate computer
and special software.

24. Ordering
Vicam IA: Can order supplies separately
as needed.
ELISA: Must order as a kit. ELISA Wells
can only be used with reagents in the kit
(matched reagents).

25. Additional Advantage
Vicam IA:
• LOD: 0.5ppb.
• Can be used with an HPLC clean up.
ELISA:
• LOD: 1 - 5 ppb
• Can’t be used for HPLC clean up

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