The document discusses how solvent quality can affect liquid chromatography performance. It explains that different grades of solvents are needed depending on the type of chromatography and detector being used, such as LC-MS, UHPLC-UV, or fluorescence detection. The key factors that determine solvent grade are discussed, including filtration level, metal ion content, UV transmission, fluorescence, and MS background noise. Various solvent grades from companies like Fisher Chemical and Thermo Scientific are presented and their applications and specifications compared. The document provides guidance on choosing the appropriate solvent for different chromatography instrumentation and techniques.
Presentation Outline for Expanding Your High Performance Liquid Chromatography and Ultra High Performance Liquid Chromatography Capabilities with Universal Detection-Shedding Light on Non-Chromophore Compounds:
• Introduction to Charged Aerosol Detection
• How Charged Aerosol Technology Works
• Comparison with Evaporative Light Scattering Detectors
(ELSD)
• Examples of Applications
• Inverse Gradient Solution for Uniform Response
Presentation Outline for Expanding Your High Performance Liquid Chromatography and Ultra High Performance Liquid Chromatography Capabilities with Universal Detection-Shedding Light on Non-Chromophore Compounds:
• Introduction to Charged Aerosol Detection
• How Charged Aerosol Technology Works
• Comparison with Evaporative Light Scattering Detectors
(ELSD)
• Examples of Applications
• Inverse Gradient Solution for Uniform Response
Determination of Carbohydrates in Various Matrices by Capillary High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)
This presentation describes the combined advantages of a reagent-free capillary format Ion Chromatography (IC) to determine monosaccharides and disaccharides in various applications, from low concentrations in synthetic urine samples to high concentrations in beverage samples. In a reagent-free IC system, the hydroxide eluent is electrolytically generated inline to deliver accurate and precise concentrations for isocratic or gradient separations by only adding deionized water. Eluent generation eliminates carbonate contamination and errors from manual preparation. A capillary scale system with µL/min flow rates can run 24/7, always on and always ready for samples.
Over the past decade, the number of mAb candidates entering the clinical pipeline has grown significantly. In addition, the number of ADCs that use mAb specificity to carry drug payloads to target sites has increased. As a result, analytical characterization is in high demand.
This webinar discusses new innovations in sample preparation, column technology, UHPLC, and high resolution mass spectroscopy (HRMS) that allow the development of analytical methods with run times of less than 5 minutes for all routine methods.
Selectivity is the KEY
Mixed-mode chromatography addresses unmet challenges in pharmaceutical analysis:
API and counter ion by RP/AEX/CEX trimodal columns (e.g. Acclaim Trinity P1 and P2)
Unique Charged Aerosol Detector for consistent response of analytes that are weak or non-chromophoric molecules
Unmatched performance for counter ion analysis by dedicated column technology, unique charged aerosol detection and established UltiMate 3000 technology
New IonCount complete solution for ease of use and quick methods development of new API and counter ion analysis
Easy operation by predefined eWorkflows, Thermo Scientific™ Dionex™ Chromeleon™ 7.2 Chromatography Data System, and viper connection tubing
The webinar is all about Ultra High Pressure Liquid Chromatography (UHPLC) performance and how new column technology can deliver the best separation power and be married with the best UHPLC system to ensure an outstanding result. It covers how chromatographers can ensure that even very complex and unfamiliar samples are assayed with the highest scrutiny possible? The webinar discusses how to get the most out of solid core column technology with the right UHPLC system. It covers the use of an extremely long column approach for ultra-high resolution assays and the outlines the importance of robustness and retention time stability.
Determination of Common Counterions and Impurity Anions in Pharmaceuticals Using a Capillary HPIC System with Suppressed Conductivity and Charge Detection
Recently, identification and quantification of ions in early stage drug development has gained increasing attention, because the APIs maybe contaminated with different counter ions from synthesis steps, and because selecting the counter ion to enhance APIs’ solubility and stability is becoming a key step in formulation development. This presentation demonstrates the identification and quantification of 22 commonly found anions in pharmaceuticals in a single run using a high-pressure capillary IC system (HPIC) with 4-μm particle ion –exchange column, and CD-QD dual detectors.
In the past, measuring the total amount of an element was sufficient. Unfortunately, the effects of an element extend far beyond its absolute amount. Different forms of an element can exhibit very different physicochemical properties, including varying toxicities. The process of separation and quantification of different chemical forms of an element, more specifically termed speciation analysis, can be utilized to determine an element’s various chemical forms. The number of environmental applications of elemental speciation analysis has increased significantly. For example, both the United States EPA and the European Union have specified maximum admissible chromium concentrations in their respective drinking water directives and are evaluating the inclusion of hexavalent chromium in certain legislation. Learn about the latest developments in chromatography technology for speciation analysis that offer data for a wide variety of applications, including chromium in drinking water and both arsenic and sulfur in environmental waters.
Different forms of an element can exhibit very different physicochemical properties, including varying toxicities. The chromatography process of separation and quantification of different chemical forms of an element, more specifically termed speciation analysis, can be utilized to determine an element’s various chemical forms. The food safety industries have significantly increased their interest in understanding an element’s various chemical forms due to pending legislative pressures. Learn about the latest developments in speciation technology and offer proof data for a wide variety of applications, including arsenic species in apple juice and rice syrup and iodine species in milk.
Monitoring and maintaining water purity are important to the power and electronics industries. In the both of these industries, impurities must be minimized and monitored to prevent corrosion or scaling, and degradation in demineralization processes. Learn about the analysis of ppb concentrations of ionic contaminants in high purity water using two easy methods: a direct large volume injection and concentration of a large volume injection, using electrolytically generated hydroxide eluents on a Reagent-Free™ Ion Chromatography system (RFIC™).
Application of ICP-MS and LC-ICP-MS in Drug DevelopmentQPS Holdings, LLC
Inductively coupled mass spectroscopy plasma (ICP-MS) has big potential in preclinical and clinical studies of new drug candidates. One particular area is metallodrugs.
An introduction to the use of ICP-MS in the clinical setting, that goes on to describe some potential new application areas for advanced instrumentation such as HPLC-ICP-MS, laser ablation-ICP-MS and immuno-tagging-ICP-MS for the measurement of biomolecules.
This webinar will provide pesticides residue analysts with valuable information on software method development and data processing for the analysis of pesticide residues in food for both LC–MS and GC–MS. Technical experts will review the latest in software advances to help with data interpretation and reporting.
High-performance anion-exchange chromatography with pulsed amperometric detection is valuable for oligosaccharide analysis with the value derived from the high-resolution separation followed by sensitive detection of native oligosaccharides. In this presentation the application of HPAE-PAD to oligosaccharides released from glycoproteins is demonstrated.
The importance of clean drinking water is recognized worldwide. In the U.S., the Environmental Protection Agency (EPA) has established Maximum Contamination Limits (MCL) for monitoring toxic contaminants that may cause adverse health effects. Other ions, such as chloride and sulfate are monitored for aesthetic characteristics under the U.S. National Secondary Drinking Water Standards guidelines. Similar regulations for clean drinking water have been implemented in other industrialized countries. Ion Chromatography (IC) methods have been approved for compliance monitoring including U.S. EPA 300.0 in 1993. Learn about using ion chromatography for the determination of inorganic anions, perchlorate and chromate for compliance monitoring according to U.S. EPA Methods 300.0, 314 and 218.6.
In the pharmaceutical arena there is great interest in solid core technology, where there is a broad range of sample types as well as requirements throughout the process of developing new chemical entities. The presentation looks at how solid core technology can be readily adapted to cope with the challenges associated with the pharmaceutical sector, looking at various sample matrices and molecular entities, from small molecules to large biomolecules. The presentation gives an insight into how varying the solid core to porous layer allows the user to optimize separation performance by reducing extra band broadening. Data presented demonstrates how this technology is more robust than fully porous systems when analyzing biological extracts, routinely used in DMPK departments, resulting in longer column lifetimes.
Analysis of Disinfection Byproducts by Ion Chromatography
In this presentation, the use of ion chromatography for the determination of bromate, chlorate and haloacetic acids for compliance monitoring according to various ISO standards (15061, 11206, 10304-4, 23631) and U.S. EPA Method 557 will be discussed. Examples will include IC methods using electrolytically generated hydroxide eluents on an RFIC™ system.
Wastewater is produced by multiple sources, including chemical manufacturing, power generation, petroleum product extraction, and private residences. Specific industries can use knowledge of around the analytes present in wastewater to make decisions on reuse, treatment, or whether disposal is the most cost effective option. Prior to any discharge into ground or surface waters, the level of specific analytes must be determined to ensure that they do not exceed regulated limits. If these limits are being exceeded, treatment will be required. Ion Chromatography (IC) is the primary technique used for measuring the concentration of ions in wastewater and numerous methods have been developed that meet regulatory requirements. Learn about IC methods that enable accurate, consistent, and rapid measurement of both anions, such as chloride, sulfate, and bromate, and cations, such as sodium and magnesium.
No single liquid chromatography (LC) detector delivers ideal results. Often with LC detectors one analyte responds more strongly than another, or may not respond at all. What is most desired is the ability to accurately measure a wide range of analytes with consistent response simultaneously.
Charged Aerosol detection (CAD) is a mass sensitive technique for determining levels of any non-volatile and many semi-volatile analytes after separation by liquid chromatography. This technique provides consistent analyte response independent of chemical characteristics and gives greater sensitivity over a wider dynamic range. An analytes response does not depend on optical properties, like with UV-vis absorbance, or the ability to ionize, as with mass spectrometry (MS). The presence of chromophoric groups, radiolabels, ionizable moieties, or chemical derivatization is needed for detection.
Determination of Carbohydrates in Various Matrices by Capillary High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)
This presentation describes the combined advantages of a reagent-free capillary format Ion Chromatography (IC) to determine monosaccharides and disaccharides in various applications, from low concentrations in synthetic urine samples to high concentrations in beverage samples. In a reagent-free IC system, the hydroxide eluent is electrolytically generated inline to deliver accurate and precise concentrations for isocratic or gradient separations by only adding deionized water. Eluent generation eliminates carbonate contamination and errors from manual preparation. A capillary scale system with µL/min flow rates can run 24/7, always on and always ready for samples.
Over the past decade, the number of mAb candidates entering the clinical pipeline has grown significantly. In addition, the number of ADCs that use mAb specificity to carry drug payloads to target sites has increased. As a result, analytical characterization is in high demand.
This webinar discusses new innovations in sample preparation, column technology, UHPLC, and high resolution mass spectroscopy (HRMS) that allow the development of analytical methods with run times of less than 5 minutes for all routine methods.
Selectivity is the KEY
Mixed-mode chromatography addresses unmet challenges in pharmaceutical analysis:
API and counter ion by RP/AEX/CEX trimodal columns (e.g. Acclaim Trinity P1 and P2)
Unique Charged Aerosol Detector for consistent response of analytes that are weak or non-chromophoric molecules
Unmatched performance for counter ion analysis by dedicated column technology, unique charged aerosol detection and established UltiMate 3000 technology
New IonCount complete solution for ease of use and quick methods development of new API and counter ion analysis
Easy operation by predefined eWorkflows, Thermo Scientific™ Dionex™ Chromeleon™ 7.2 Chromatography Data System, and viper connection tubing
The webinar is all about Ultra High Pressure Liquid Chromatography (UHPLC) performance and how new column technology can deliver the best separation power and be married with the best UHPLC system to ensure an outstanding result. It covers how chromatographers can ensure that even very complex and unfamiliar samples are assayed with the highest scrutiny possible? The webinar discusses how to get the most out of solid core column technology with the right UHPLC system. It covers the use of an extremely long column approach for ultra-high resolution assays and the outlines the importance of robustness and retention time stability.
Determination of Common Counterions and Impurity Anions in Pharmaceuticals Using a Capillary HPIC System with Suppressed Conductivity and Charge Detection
Recently, identification and quantification of ions in early stage drug development has gained increasing attention, because the APIs maybe contaminated with different counter ions from synthesis steps, and because selecting the counter ion to enhance APIs’ solubility and stability is becoming a key step in formulation development. This presentation demonstrates the identification and quantification of 22 commonly found anions in pharmaceuticals in a single run using a high-pressure capillary IC system (HPIC) with 4-μm particle ion –exchange column, and CD-QD dual detectors.
In the past, measuring the total amount of an element was sufficient. Unfortunately, the effects of an element extend far beyond its absolute amount. Different forms of an element can exhibit very different physicochemical properties, including varying toxicities. The process of separation and quantification of different chemical forms of an element, more specifically termed speciation analysis, can be utilized to determine an element’s various chemical forms. The number of environmental applications of elemental speciation analysis has increased significantly. For example, both the United States EPA and the European Union have specified maximum admissible chromium concentrations in their respective drinking water directives and are evaluating the inclusion of hexavalent chromium in certain legislation. Learn about the latest developments in chromatography technology for speciation analysis that offer data for a wide variety of applications, including chromium in drinking water and both arsenic and sulfur in environmental waters.
Different forms of an element can exhibit very different physicochemical properties, including varying toxicities. The chromatography process of separation and quantification of different chemical forms of an element, more specifically termed speciation analysis, can be utilized to determine an element’s various chemical forms. The food safety industries have significantly increased their interest in understanding an element’s various chemical forms due to pending legislative pressures. Learn about the latest developments in speciation technology and offer proof data for a wide variety of applications, including arsenic species in apple juice and rice syrup and iodine species in milk.
Monitoring and maintaining water purity are important to the power and electronics industries. In the both of these industries, impurities must be minimized and monitored to prevent corrosion or scaling, and degradation in demineralization processes. Learn about the analysis of ppb concentrations of ionic contaminants in high purity water using two easy methods: a direct large volume injection and concentration of a large volume injection, using electrolytically generated hydroxide eluents on a Reagent-Free™ Ion Chromatography system (RFIC™).
Application of ICP-MS and LC-ICP-MS in Drug DevelopmentQPS Holdings, LLC
Inductively coupled mass spectroscopy plasma (ICP-MS) has big potential in preclinical and clinical studies of new drug candidates. One particular area is metallodrugs.
An introduction to the use of ICP-MS in the clinical setting, that goes on to describe some potential new application areas for advanced instrumentation such as HPLC-ICP-MS, laser ablation-ICP-MS and immuno-tagging-ICP-MS for the measurement of biomolecules.
This webinar will provide pesticides residue analysts with valuable information on software method development and data processing for the analysis of pesticide residues in food for both LC–MS and GC–MS. Technical experts will review the latest in software advances to help with data interpretation and reporting.
High-performance anion-exchange chromatography with pulsed amperometric detection is valuable for oligosaccharide analysis with the value derived from the high-resolution separation followed by sensitive detection of native oligosaccharides. In this presentation the application of HPAE-PAD to oligosaccharides released from glycoproteins is demonstrated.
The importance of clean drinking water is recognized worldwide. In the U.S., the Environmental Protection Agency (EPA) has established Maximum Contamination Limits (MCL) for monitoring toxic contaminants that may cause adverse health effects. Other ions, such as chloride and sulfate are monitored for aesthetic characteristics under the U.S. National Secondary Drinking Water Standards guidelines. Similar regulations for clean drinking water have been implemented in other industrialized countries. Ion Chromatography (IC) methods have been approved for compliance monitoring including U.S. EPA 300.0 in 1993. Learn about using ion chromatography for the determination of inorganic anions, perchlorate and chromate for compliance monitoring according to U.S. EPA Methods 300.0, 314 and 218.6.
In the pharmaceutical arena there is great interest in solid core technology, where there is a broad range of sample types as well as requirements throughout the process of developing new chemical entities. The presentation looks at how solid core technology can be readily adapted to cope with the challenges associated with the pharmaceutical sector, looking at various sample matrices and molecular entities, from small molecules to large biomolecules. The presentation gives an insight into how varying the solid core to porous layer allows the user to optimize separation performance by reducing extra band broadening. Data presented demonstrates how this technology is more robust than fully porous systems when analyzing biological extracts, routinely used in DMPK departments, resulting in longer column lifetimes.
Analysis of Disinfection Byproducts by Ion Chromatography
In this presentation, the use of ion chromatography for the determination of bromate, chlorate and haloacetic acids for compliance monitoring according to various ISO standards (15061, 11206, 10304-4, 23631) and U.S. EPA Method 557 will be discussed. Examples will include IC methods using electrolytically generated hydroxide eluents on an RFIC™ system.
Wastewater is produced by multiple sources, including chemical manufacturing, power generation, petroleum product extraction, and private residences. Specific industries can use knowledge of around the analytes present in wastewater to make decisions on reuse, treatment, or whether disposal is the most cost effective option. Prior to any discharge into ground or surface waters, the level of specific analytes must be determined to ensure that they do not exceed regulated limits. If these limits are being exceeded, treatment will be required. Ion Chromatography (IC) is the primary technique used for measuring the concentration of ions in wastewater and numerous methods have been developed that meet regulatory requirements. Learn about IC methods that enable accurate, consistent, and rapid measurement of both anions, such as chloride, sulfate, and bromate, and cations, such as sodium and magnesium.
No single liquid chromatography (LC) detector delivers ideal results. Often with LC detectors one analyte responds more strongly than another, or may not respond at all. What is most desired is the ability to accurately measure a wide range of analytes with consistent response simultaneously.
Charged Aerosol detection (CAD) is a mass sensitive technique for determining levels of any non-volatile and many semi-volatile analytes after separation by liquid chromatography. This technique provides consistent analyte response independent of chemical characteristics and gives greater sensitivity over a wider dynamic range. An analytes response does not depend on optical properties, like with UV-vis absorbance, or the ability to ionize, as with mass spectrometry (MS). The presence of chromophoric groups, radiolabels, ionizable moieties, or chemical derivatization is needed for detection.
AAVOS International bvba has a wide range of online process instrumentation for many applications in chemical, petrochemical, oil, gas, food, beverage and pharmaceutical industry.
We will advise you to find the right equipment for your analysis of fluids, gasses and solids.
Process on-line analysers are offered as a solution to the efficiency of plant operation and offer huge paybacks. Our analysers are also applied for monitoring emissions and as safety measurement. http://aavos.be
At GVK BIO, we provide a full range of analytical services from Discovery to Commercial Phase III. The Analytical Service portfolio includes method development and validation, stability studies, analytical testing and release, structure elucidation, GMP separation and CMC Support
A catalog including carefully selected products, from the most prestigious brands in the Water Treatment market, operative website, personalized services, precise and efficient logistics, flexible organization, but also the basic importance that is attributed to human factors and relationships with partners, make of Sinergroup Srl a reference for many companies of the market.
Outline of presentation:
Overview — Plating Baths and High Pressure Liquid Chromatography (HPLC)
Determination of Accelerator and Suppressor by HPLC and Charged Aerosol Detection
Sample Preparation, Calibration, Measurements
Comparisons to CVS data
Determination of Accelerator and Leveller by HPLC and Electrochemical Detection (ECD)
Coulometric Detection Mechanism and Design
Calibration and Measurements
Nickel Additives, Saccharin and Sodium Alkylsulfate
Gage Study Results
This webinar will provide pesticides residue analysts with valuable information on the development and optimization of chromatographic separations and mass spectrometry methods for the analysis of pesticide residues in food. The expert speakers will share their knowledge in understanding the critical aspects of the method, assisting analysts in optimizing their methods for the most challenging analyses.
Have a Good Trip? How to Analyze PsychedelicsMarkus Roggen
Entheogens, frequently used interchangeably with hallucinogens and psychedelics, are naturally occurring psychoactive substances. Historically, these substances have been used to induce a change of perception, mood, consciousness, or behavior for the purposes of spiritual development or social enrichment. In addition to these uses, these substances have become strong candidates for alternative medicines for the treatment of psychological disorders. Cannabis, a more common entheogen, has paved the way for decriminalization and legalization of other substances, such as psilocin, psilocybin, MDMA, and LSD to be used for clinical treatments. While these compounds in many places are still classified as Schedule I substances under the Controlled Substances Act, other entheogenic plants with different psychoactive compounds are not, such as Mitragyna speciosa and Amanita muscaria, which already appear on the market in the form of edibles, extracts, or powders. With the lack of standardized methods for these psychoactive targets, and with the rise of legalization and clinical treatment centers, it is important to understand this emerging market from a safety and quality perspective. This session will focus on emerging products, testing targets, sample preparation, method development, and analytical challenges. Attendees of this session will learn technical steps that need to be considered to develop robust, reproducible analytical methods, and a quality assurance program to expand into this new and evolving area of testing.
This presentation lays out all the differences between the two analytical techniques i.e. High Performance Thin Layer Chromatography and High Performance Liquid Chromatography.
For more information regarding HPTLC, please visit www.anchrom.in or you can send us an email at lab@anchrom.in
Thank you
Anchrom
Absolute quantification of mAbs and ADCs : forget amino acid analysis and shi...Quality Assistance s.a.
Absolute quantification of mAbs and ADCs: Quality Assistance developed an innovative analytical method based on sulfur quantification by ICP-MS/MS
Visit www.quality-assistance.com for more information
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
The Art of the Pitch: WordPress Relationships and SalesLaura Byrne
Clients don’t know what they don’t know. What web solutions are right for them? How does WordPress come into the picture? How do you make sure you understand scope and timeline? What do you do if sometime changes?
All these questions and more will be explored as we talk about matching clients’ needs with what your agency offers without pulling teeth or pulling your hair out. Practical tips, and strategies for successful relationship building that leads to closing the deal.
Software Delivery At the Speed of AI: Inflectra Invests In AI-Powered QualityInflectra
In this insightful webinar, Inflectra explores how artificial intelligence (AI) is transforming software development and testing. Discover how AI-powered tools are revolutionizing every stage of the software development lifecycle (SDLC), from design and prototyping to testing, deployment, and monitoring.
Learn about:
• The Future of Testing: How AI is shifting testing towards verification, analysis, and higher-level skills, while reducing repetitive tasks.
• Test Automation: How AI-powered test case generation, optimization, and self-healing tests are making testing more efficient and effective.
• Visual Testing: Explore the emerging capabilities of AI in visual testing and how it's set to revolutionize UI verification.
• Inflectra's AI Solutions: See demonstrations of Inflectra's cutting-edge AI tools like the ChatGPT plugin and Azure Open AI platform, designed to streamline your testing process.
Whether you're a developer, tester, or QA professional, this webinar will give you valuable insights into how AI is shaping the future of software delivery.
Elevating Tactical DDD Patterns Through Object CalisthenicsDorra BARTAGUIZ
After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more ‘mechanical’ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
Neuro-symbolic is not enough, we need neuro-*semantic*Frank van Harmelen
Neuro-symbolic (NeSy) AI is on the rise. However, simply machine learning on just any symbolic structure is not sufficient to really harvest the gains of NeSy. These will only be gained when the symbolic structures have an actual semantics. I give an operational definition of semantics as “predictable inference”.
All of this illustrated with link prediction over knowledge graphs, but the argument is general.
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
Speakers:
👨🏫 Andras Palfi, Senior Product Manager, UiPath
👩🏫 Lenka Dulovicova, Product Program Manager, UiPath
FIDO Alliance Osaka Seminar: Passkeys at Amazon.pdf
Solvent affecting chromatography 20 min
1. The world leader in serving scienceProprietary & Confidential
Jan Pettersson
Nordic HPLC & Chromeleon support
How solvent quality affects your analysis
5. 5
How solvent quality affects your analysis
1- Why there is a need for
different grade of solvents ?
2- What can affect the
chromatography performance?
Not all solvents are equal
7. 7
HPLC Gradient grade
• The economical choice for HPLC
gradient grade for UV
UHPLC-UV Gradient grade
• The good choice for UHPLC-UV
Thermo Scientific
UltiMate™ 3000
UV detector
LC instrumentation Driven Solvent Quality Needs
8. 8
HPLC Gradient grade
• The economical choice for HPLC
gradient grade for UV
UHPLC-UV Gradient grade
• The good choice for UHPLC-UV
Thermo Scientific
UltiMate™ 3000
UV detector
Thermo Scientific UltiMate™ 3000
With TSQ or Q Exactive…
Optima LC-MS
• For high sensitivity LC-MS analysis
• Better quality than LC-MS grade
• UV scan from 200-400 nm
• Low metal impurities
LC instrumentation Driven Solvent Quality Needs
9. 9
HPLC Gradient grade
• The economical choice for HPLC
gradient grade for UV
UHPLC-UV Gradient grade
• The good choice for UHPLC-UV
Thermo Scientific
UltiMate™ 3000
UV detector
Thermo Scientific UltiMate™ 3000
With TSQ or Q Exactive…
Thermo Scientific Vanquish™
With TSQ or Q Exactive or Orbitrap…& CAD
Optima LC-MS
• For high sensitivity LC-MS analysis
• Better quality than LC-MS grade
• UV scan from 200-400 nm
• Low metal impurities
UHPLC-MS
• For UHPLC-MS applications with highest
sensitivity
• Higher quality than Optima LC-MS
• Lowest metal impurities
• Low baseline noise in MS detector results in higher
signal to noise ratios.
LC instrumentation Driven Solvent Quality Needs
10. 10
How solvent quality affects your analysis
Key Questions
LC or UHPLC
UV ?
Fluorescence ?
CAD ?
MS ?
11. 11
LC-MS
• Low mass noise levels
• Lowest UV absorbing impurity background
• Low metal ions content
• Ultrafiltration level
UHPLC-MS
• Lowest UV absorbing impurity & MS-ionizing impurities
background
• Lowest metal ions content
• Optimal signal to noise ration in MS/MS mode
• Ultrafiltration for demanding UHPLC-MS application
LC-UV
• High UV Transmission
• Low acidity & alkalinity level
• Filtered to 0,2µm
UHPLC-UV
• Outstanding high UV transmission, low acidity & alkalinity level
• Ultrafiltration to 0,1µm
UHPLC-CAD
• Low mass noise levels
• Minimal organic contamination
• Minimal metal content
• Low presence of non volatile dissolved
contaminants
Key criteria required per instruments
17. 17
Fluorescence Detectors
What is important
High UV transmission
Ultra filtrated to at least 0,2µm
Low fluorescence between 250nm and
750nm emission & excitation
wavelengths
19. 19
Fluorescence
Comparison of the fluorescence baseline behavior of different grade water samples
(Pink: purified water, blue: ultra-pure lab water, black: LC/MS grade water
29. 29
MS requirement
Application Grade
LC-UV Gradient Test
Diode array detector
Signal to Noise Metal tested Packaging
LC-MS
Optima LC-MS
UHPLC-UV Suitability
test
Scan from 200 to 400nm
No test
17 Amber Glass bottle
UHPLC-MS
UHPLC-MS
UHPLC-MS suitability
test
Scan from 200 to 400nm
Signal to noise ratio of
production peak (MS/MS)
250 ppt propazine
(m/z 188) > 10 S/N
17
Borosilicate glass bottle
Reducing significantly
leaching of metal cations
( Na+ & K+)
• Lowest UV absorbing impurity & MS-ionizing impurities background
• Lowest metal ions content
• Optimal signal to noise ration in MS/MS mode
30. 30
How solvents quality affect your LC-MS performance
Black is the LCMS ( Optima LC-MS Fisher Chemical )
Blue line is UHPLCMS ( UHPLC-MS Thermo Scientific )
UHPLC-MS quality is much better vs LCMS , background noise is lower meaning much
better quality for the Corona detector sensitivity
31. 31
Summary
HPLC or UHPLC
• UV absorbance characteristics
• particles content and size
LC-MS or UHPLC-MS
• UV & MS-ionizing impurities background
• Metal ion content
• Particle content and size of the particles
32. 32
System cleaning
What can affect the
chromatography
performance?
Phtalates
Polypropylene glycol -PPG
Polyethylene glycol - PEG
Siloxanes
33. 33
System cleaning
Clean up contaminated LC & LC-MS instruments
Reduce background noise
Facilitate instrument installation, preventative maintenance
Help to avoid troubleshoot instruments
• Effective removal of common background contamination such as:
• Polyethylene glycol usually observed in ESI+ as a series of ions separated by 44 Da
• Polypropylene glycol usually seen in ESI+ as a series of ions separated by 58 Da
• Phthalates observed as [M+H]+ in ESI+ at m/z = 391, 419 and 447 which are attributed to
• diisooctylphthalate, dinonylphthalate, diisodecyl phthalate, respectively
• Siloxanes observed at m/z = 445, 519, 593, 667 and 741
34. 34
Thermo Scientific ChromaCare™ LC-MS Flush Solution
• Assay by GC-TCD is 25% 2%
• Color ≤ 10 APHA
• Residue ≤ 1mg/L
• Optical Absorbance at 210nm ≤ 1.0 AU
• Optical Absorbance at 220nm ≤ 0.5 AU
• Optical Absorbance at 254nm ≤ 0.05 AU
• Calcium (Ca) ≤ 20ppb
• Potassium (K) ≤ 10ppb
• Sodium (Na) ≤ 50ppb
New code T11110 ( 1L)
• Formulation:
Acetonitrile 25%
Methanol 25%
Isopropanol 25%
Water 25%
35. 35
Common Background Contamination Ions in Mass Spectrometry
Cleaning out:
Polyethylene glycol, polypropylene
glycol, phthalates, organic solvent
clusters, solvent modifiers, fatty
acids, metal ions, tritons, tweens
and siloxanes.
Metal ions form adducts with
varying numbers of substrates to
give characteristic ESI+ ions.
39. 39
LC instrumentation Driven Solvent Quality Needs
Chromatography Application Instrument and Detector Type
Fisher Chemical & Thermo
Scientific Solvent Grade
UHPLC-MS UHPLC coupled with Mass detector or CAD UHPLC-MS Optima
HPLC-MS LC coupled with Mass detector Optima LC-MS HPLC
UHPLC-UV UHPLC coupled with UV detector UHPLC Gradient Grade
HPLC gradient analysis LC gradient coupled with UV detector HPLC Gradient Grade
HPLC Fluorescence UHPLC/LC gradient coupled with FL detector HPLC Florescence
40. 40
Filtration requirement in chromatography
Application Filtered to
Fisher Chemical
Thermo Scientific
UHPLC-MS
0,05µm (acetonitrile & methanol )
0,03µm water
UHPLC-MS grade Optima
HPLC-MS
0.1 µm (acetonitrile & methanol )
0.03 µm (Water only) Optima LC-MS grade
UHPLC-UV To 0,1 µm UHPLC Gradient Grade
HPLC gradient analysis To 0,2µm HPLC Gradient Grade
41. 41
Laboratory Chemicals | Portfolio Overview
Organic and
inorganic chemistry
Life science Screening libraries
Reagents used to
synthesize a
compound or material.
Focused on high purity
inorganics, metals and
materials, building
blocks, and moisture
sensitive reagents.
Eliminates the need
for testing with
essential reagents
prequalified for
molecular biology,
protein chemistry, and
cell biology
World-leading
screening libraries
and compound
collections of diverse
drug-like molecules
designed to
accelerate the drug
discovery process
For Research Use Only. Not for use in diagnostic procedures
Analytical
chemistry
A full portfolio of dry
reagents, salts, acids,
solutions, and high-
purity solvents ready
to analyze and
purify a compound or
material.
Specialized
chemical services
Custom chemical,
testing, or packaging
solutions in research
to scaling up/
development for
production
42. 42
UHPLC-MS Optima™
Grade
UHPLC-MS Optima™
Application
UHPLC-MS
Definition
Ultra high-purity solvents specifically qualified for UHPLC-MS instrumentation. Specification based on higher
ionization efficiency to detect organic contaminants in full scan MS with the absence of an additive. Signal to
noise specification greater than ten when measured with 250 ppt Propazine using MS/MS. Filtered at 0.1μm,
packaged in borosilicate glass and tightened metal specifications minimizes metal ion adduct formation
Designed to ensure:
• low metal ion adduct formation
• reduce column clogging
• improve peak profiles
43. 43
Optima LC-MS
Grade
Optima LC-MS
Application
LC-MS
Definition
Optima LC-MS grade products meet stringent purity requirements of LC/MS and UHPLC by addressing
the need for minimal organic contamination with 0.1μm filtration to make particle free. Evaluated for 17
metal impurities at ppb concentrations for minimal metal mass adduct formation. High ionization
efficiency to detect organic contaminants at 50 ppb max (positive) and 300 ppb max (negative) in full
scan MS. Screened for UV-absorbing contaminants at every wavelength in the 200 to 400 nm range to
afford smooth baselines and to reduce interferences
44. 44
Grade
LC-MS
Application
LC-MS
Definition
Ideal mobile phase for routine LC-MS applications. Guaranteed for low level of trace metals and nonvolatile
residue. Low level of absorbance, performance under gradient conditions. Filtered at 0.2μm
LC-MS
45. 45
Advanced HPLC Gradient grade
Grade
Advanced HPLC Gradient grade
Application
HPLC Gradient grade
Definition
Advanced HPLC gradient grade specifically manufactured to guarantee a very low level of gradient baseline
drift. Includes lot analysis and absorbance curve on the label. Filtered at 0.2μm.
46. 46
UHPLC Gradient grade
Grade
UHPLC Gradient grade
Application
UHPLC-UV
Definition
Advanced HPLC gradient grade specifically manufactured to guarantee a very low level of gradient baseline
drift. Includes lot analysis and absorbance curve on the label. Filtered at 0.2μm.
47. 47
HPLC Gradient grade
Grade
HPLC Gradient grade
Application
HPLC Gradient grade
Definition
HPLC solvents suitable for gradient analysis. Guaranteed for low absorbance/high UV transmission and low
concentration of non-volatile impurities. In some instances may be suitable for fluorescence detection.
Includes lot analysis and absorbance curve on the label. Filtered at 0.2μm.
48. 48
HPLC Fluorescence
Grade
HPLC Fluorescence
Application
HPLC with Fluorescence and UV detectors
Definition
HPLC solvents suitable for Fluorescence and UV detectors. Guaranteed for low fluorescence between 250nm
and 750nm emission & excitation wavelengths.
49. 49
HPLC Electrochemical
Grade
HPLC Electrochemical
Application
HPLC with Electrochemical and UV detectors
Definition
HPLC solvents suitable for Electrochemical and UV detectors. Guaranteed for low electrochemical activity
and low UV absorbance/high transmission. Includes lot analysis and absorbance curve on label.
