






Genetic material of living organisms is either
DNA or RNA.
DNA – Deoxyribonucleic acid
RNA – Ribonucleic acid
Genes are lengths of DNA that code for
particular proteins.

Monday, December 09,
2013

Pranabjyoti Das

1





Both DNA and RNA are polynucleotides.
They are made up of smaller molecules called
nucleotides.
DNA is made of two polynucleotide strands:

Nucleotide

Nucleotide

Nucleotide

Nucleotide

Nucleotide

Nucleotide

Nucleotide

Nucleotide

Nucleotide

Nucleotide



RNA is made of a single polynucleotide strand:

Nucleotide

Nucleotide

Monday, December 09,
2013

Nucleotide

Pranabjyoti Das

Nucleotide

Nucleotide

2
A nucleotide is made of 3
components:
 A Pentose sugar
 This is a 5 carbon
sugar
 The sugar in DNA is
deoxyribose.
 The sugar in RNA is
ribose.

Monday, December 09,
2013

Pranabjyoti Das

3



A Phosphate group
Phosphate groups are
important because
they link the sugar on
one nucleotide onto
the phosphate of the
next nucleotide to
make a
polynucleotide.

Monday, December 09,
2013

Pranabjyoti Das

4


A Nitogenous base



In DNA the four bases are:
ļ‚”
ļ‚”
ļ‚”
ļ‚”



Thymine
Adenine
Cytosine
Guanine

In RNA the four bases are:
ļ‚”
ļ‚”
ļ‚”

ļ‚”

Uracil
Adenine
Cytosine
Guanine

Monday, December 09,
2013

Pranabjyoti Das

5
Pyramidines

Purines

Thymine - T
Cytosine - C
Uracil - U

Adenine - A
Guanine - G

Monday, December 09,
2013

Pranabjyoti Das

6
Monday, December 09,
2013

Pranabjyoti Das

7
Monday, December 09,
2013

Pranabjyoti Das

8
Monday, December 09,
2013

Pranabjyoti Das

9




Nucleotides are
connected to each other
via the phosphate on
one nucleotide and the
sugar on the next
nucleotide
A Polynucleotide

Monday, December 09,
2013

Pranabjyoti Das

10
Monday, December 09,
2013

Pranabjyoti Das

11


The Nitrogenous
Bases pair up with
other bases. For
example the bases of
one strand of DNA
base pair with the
bases on the opposite
strand of the DNA.

Monday, December 09,
2013

Pranabjyoti Das

12
Monday, December 09,
2013

Pranabjyoti Das

13
Monday, December 09,
2013

Pranabjyoti Das

14
Monday, December 09,
2013

Pranabjyoti Das

15






Adenine always base pairs with Thymine (or
Uracil if RNA)
Cytosine always base pairs with Guanine.

This is beacuse there is exactly enough room
for one purine and one pyramide base between
the two polynucleotide strands of DNA.

Monday, December 09,
2013

Pranabjyoti Das

16
Purines

Pyramidines

Adenine
Adenine

Thymine
Uracil

Guanine

Cytosine

Monday, December 09,
2013

Pranabjyoti Das

17






Property 1 - it must contain, in a stable form,
information encoding the organism’s
structure, function, development and
reproduction
Property 2 - it must replicate accurately so
progeny cells have the same genetic makeup
Property 3 - it must be capable of some
variation (mutation) to permit evolution

Monday, December 09,
2013

Pranabjyoti Das

18




Speed of DNA replication:
3,000 nucleotides/min in human
30,000 nucleotides/min in E.coli
Accuracy of DNA replication:
Very precise (1 error/1,000,000,000 nt)

Monday, December 09,
2013

Pranabjyoti Das

19
Monday, December 09,
2013

Pranabjyoti Das

20
Monday, December 09,
2013

Pranabjyoti Das

21
Monday, December 09,
2013

Pranabjyoti Das

22
3H-labelled

after one further replication
Pranabjyoti Das
in unlabelled media 23

chromosomes

Monday, December 09,
2013
Monday, December 09,
2013

Pranabjyoti Das

24
Monday, December 09,
2013

Pranabjyoti Das

25
Monday, December 09,
2013

Pranabjyoti Das

26
Origins initiate
replication at
different
times.

Monday, December 09,
2013

Pranabjyoti Das

27
Monday, December 09,
2013

Pranabjyoti Das

28
Monday, December 09,
2013

Pranabjyoti Das

29
Monday, December 09,
2013

Pranabjyoti Das

30
Monday, December 09,
2013

Pranabjyoti Das

31
Monday, December 09,
2013

Pranabjyoti Das

32
Monday, December 09,
2013

Pranabjyoti Das

33
Monday, December 09,
2013

Pranabjyoti Das

34
Monday, December 09,
2013

Pranabjyoti Das

35
Monday, December 09,
2013

Pranabjyoti Das

36

Structure of DNA