Reverse transcription is the process by which retroviruses such as HIV convert their RNA genomes into DNA using the reverse transcriptase enzyme. The process involves an oligo dT primer binding to mRNA, followed by reverse transcriptase copying the first cDNA strand while the mRNA is intact, then digesting the mRNA and displacing it to copy the second cDNA strand, ultimately producing double-stranded cDNA. The presentation discusses reverse transcription as it relates to retroviruses and the multi-step process by which reverse transcriptase converts viral RNA into DNA.

3. RNA & Reverse Transcription
Presented By :- Farah Arain
Reg. No. :- 2k15-PD-79
Assigned By :- Dr. Shabana Memon
(Associate Professor Dept. of Plant Breeding &
Genetics).

6. Concept of Reverse Transcription
 The idea of reverse transcription
is based upon retroviruses.
 Retroviruses have an RNA
rather than DNA genome.
 So they must use reverse transcription
to convert their RNA genomes into DNA.
 Human Immunodeficiency Virus (HIV)
is an example of a retrovirus.

7. Reverse Transcription Process
 In 1st step Oligo dT primer is bound to mRNA.
 In 2nd step reverse transcriptase (RT enzyme) copies the 1st cDNA strand.

8.  In the 3rd step reverse transcriptase digests and displaces mRNA and
copies 2nd strand of cDNA.
 Finally a double stranded cDNA comes into existence.

10. THANKS