Stereo microscope fluorescence illumination solution
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Solution to upgrade stereo microscope to fluorescence microscope observing GFP, mCherry and DAPI. Mshot stereo fluorescence illuminator for Olympus, Nikon, Leica and Zeiss stereomicroscopy.
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TEM uses electron beams to image materials at high magnifications and resolutions. It works by transmitting electrons through a thin sample and forming an image from the electrons. Different imaging modes like bright field and dark field are used by selecting certain electron signals using an aperture. Electron diffraction is also possible, allowing analysis of crystal structures and orientations. Sample preparation into thin foils is important. TEM can reveal details of microstructures like defects, phases, and interfaces.
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This document summarizes an experiment using SEM and AFM microscopy to image and characterize multi-walled carbon nanotubes (MWCNTs). SEM imaging provided estimated diameters of 60.9nm and lengths of 3.21um for the MWCNTs. AFM imaging was unsuccessful likely due to errors in MWCNT preparation that left unwanted material like calcium carbonate binding to the nanotubes, interfering with AFM parameter adjustments needed for clear imaging. While SEM imaging worked as expected for the conductive carbon nanotubes, AFM imaging requires a more uniform sample to produce high-quality images.
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Night vision technology allows humans to see in low light conditions using either image intensification or thermal imaging. Image intensification works by amplifying existing light so that the user can see farther on a moonless night than with the naked eye. There are three main types of night vision devices: scopes, goggles, and cameras, which are used for military operations, hunting, security, and navigation applications. While night vision provides advantages for seeing in darkness, its initial costs can be high.
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Fluorescence microscopy uses fluorescent dyes and ultraviolet light to study samples. When exposed to UV light, the dyes become excited and emit light of longer wavelengths. The microscope filters out the UV light and passes the emitted light through to view fluorescent specimens. Applications include using fluorescent dyes to tag and identify microbes, parasites, and antigens or antibodies in immunofluorescence techniques.
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A fluorescence microscope uses fluorescence to enhance its capabilities beyond a regular light microscope. It illuminates samples tagged with fluorescent dyes with high-energy light, which causes the dyes to emit lower-energy light, producing a magnified image. This allows visualization of cell structures and live/dead cell assays. Advanced fluorescence microscopes like confocal microscopes can generate high-resolution 3D images of sample depths using lasers and image reconstruction software. Key applications include imaging cellular components, viability studies, and fluorescence in situ hybridization.
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Examples of Various Imaging Techniques- SEM, AFM, TEM and Fluorescence
Examples of Various Imaging Techniques- SEM, AFM, TEM and Fluorescence
Similar to Stereo microscope fluorescence illumination solution
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The pdf contain all the information of various technique ,such as chromatography,spectroscopy,centrifugation,electrophoresis special thanks to Dr.Rambir Singh for helping out the topics easily.Contact for help or suggestion @7985214648 whattapp only
Microscopy
Eye microscopy and electron microscopy include differentiation and reflection. Retraction of magnetic fields/electron beams that interact with the image. As well as the scattering of scattered rays or other signals to create the image.
This procedure can be done by inserting a wide-field light sample or by scanning a fine beam over the sample. A microscopy scan probe involves. The interaction of the scanning probe with the surface of the object of interest.
Advances in microscopy transformed living things and exposed the field of histology. And so remain an important strategy for health and natural science.
X-ray microscopy is three-dimensional and unobtrusive. Allowing for repeated photographing of the same sample in situ or 4D subjects. And provides the ability to "see". The sample is readable before devoting it to advanced correction techniques.
The 3D X-ray microscope uses a computed tomography technique, rotating the sample. By 360 degrees and reconstructing images. CT is usually done with a flat panel display. The 3D X-ray microscope uses a series of objectives, e.g., from 4X to 40X, and can include a flat panel.
History of Microscopy
The field of the microscope dates back to at least the 17th century. Early mirrors, single-lens magnifying glasses with limited size. Back to the widespread use of eyeglasses in the 13th century. But the most advanced microscopes first appeared in Europe around 1620 Early.
Microscope doctors included Galileo Galilei, who was discovered in 1610. That he could turn off his telescope to see small objects nearby. And Cornelis Drebbel. Who may have invented the compact microscope in about 1620?
Antonie van Leeuwenhoek developed a simple magnifying microscope. In the 1670s and is often regarded as the first acclaimed microscopist and microbiologist.
Microscope Uses
to view bacteria, parasites, and a variety of human/animal cells
cellular process, cell division
DNA replication
tissue analysis
examining forensic evidence
studying the role of a protein within a cell
studying atomic structures
And in what way are bacteria able to infect human cells, then we use a microscope to study them all. Those studies are done at the micro-level.
We use a microscope to perform the kind of study that we cannot see with the naked eye.
Microscope component
Light
Lence
Optical/Light Microscopy
Bright Field Microscopy
Dark Field Microscopy
Confocal Microscopy
Phase Contrast Microscopy
Fluorescence Microscopy
Electron microscopy
Transmission Electron Microscopy
Scanning Electron Microscopy
Scanning Probe Microscopy
The resolving power of a microscope means
Confocal microscopy by prabhat
Confocal microscopy was invented by Marvin Minsky in 1957 and aims to improve resolution over traditional microscopy. It uses point illumination and a pinhole to exclude out-of-focus light and produce thin optical sections and high-contrast images. The key components are a laser light source, dichromatic mirror, pinholes, and photodetector. Confocal microscopy finds applications in cell biology and materials science by allowing optical sectioning and 3D reconstruction. It provides advantages like non-invasiveness, live cell imaging, and depth analysis, but has disadvantages such as photobleaching and loss of intensity.
Microscope and types of microscope
This document discusses different types of microscopes, including their history, parts, uses, and key features. It describes:
1) The early compound microscope invented by Jansen, capable of 3-9x magnification. Compound microscopes can magnify 40-1000x and have a resolution of 0.25um.
2) Electron microscopes, which use electron beams rather than light and have much higher resolutions of 1-10nm. Scanning electron microscopes provide 3D images while transmission electron microscopes have higher magnification but only show black and white 2D images.
3) Other microscope types like binocular, darkfield, and phase contrast microscopes and their applications in biology research. Pre
Microscope
Introduction to microscopy
Different parts of a microscope & their function
Different types of microscopy
Different types of optical microscopy
Different types of electron microscopy
Different terms used in microscopy
Staining- Simple, Differential, Special
Gram Staining
Confocal microscopy
Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light.
Bright-field microscopy is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light and contrast in the sample is caused by absorbance of some of the transmitted light in dense areas of the sample.
Microscopy .
The document discusses microscopy techniques. It begins by defining microscopy as the technique of viewing tiny objects that are too small to see with the naked eye. It then covers the basic principles of microscopy including resolution, magnification, and illumination. The major components of light microscopes are described as the light source, stand, stage, objective lens, and eyepiece lens. Different types of light microscopes are outlined including brightfield, darkfield, phase contrast, and fluorescence microscopes. Applications of microscopy in various fields like molecular imaging, cellular imaging, and biomedical engineering are also summarized.
Confocal Microscopy.pptx MSC BIOTECHNOLOGY
Confocal microscopy is an optical imaging technique that increases resolution and contrast in micrographs. It works by using a pinhole to block out-of-focus light and only allow focused light to be detected. This allows the reconstruction of 3D structures from multiple 2D images taken at different depths. Key components include a laser, mirrors to direct the laser light, a pinhole aperture, and detector. It works by scanning a focused point of laser light across the sample and detecting the emitted light through the pinhole from in-focus points only. This provides advantages like better resolution and ability to collect 3D image data. Confocal microscopy has applications in fields like biology, materials science, and semiconductor inspection.
Microscopy
The document discusses different types of microscopes, including compound microscopes and stereomicroscopes. It describes the key parts and principles of operation. Compound microscopes use multiple lenses to magnify specimens and provide a two-dimensional image. Stereomicroscopes use two optical paths to provide a three-dimensional view of surface details. Examples of uses include biology studies, forensics, manufacturing quality control, and more. The document also discusses who may have invented the compound microscope and provides references for further reading.
Confocal microscope
The document summarizes the history and components of the confocal microscope. It describes how the confocal microscope was initially conceived in the 1950s but lacked the necessary light sources and computing power. Work in the late 1960s adapted the original concept and allowed for the examination of unstained brain and ganglion cells. Further developments in lasers and computing through the 1980s led to more practical confocal microscopes. Modern confocal microscopes integrate optics, detectors, computers and lasers to produce high-resolution 3D electronic images of samples. Confocal microscopes are now used across various fields including biology and medicine.
Microscopic techniques
Microscopic techniques which are used in lab and it is very helpful in exam preapartion also and details study about each and every techniques.
Stereo Microscope or Dissecting Miscrscope
The stereo microscope is an optical microscope variant designed for low magnification observation of surface samples. It has three key parts: a viewing head/body that houses optical components, a focus block that attaches the head to the stand, and a luminous stand that supports the microscope. Stereo microscopes provide an erect, three-dimensional perspective and are used for applications like insect dissection, microsurgery, watchmaking, and commercial inspection tasks.
Microscope and Microscopy
Microscope and Microscopy
Principal , Function & Difference of various types of Light & Electron microscope.Microscopy is the technical field of using microscopes to view samples & objects that cannot be seen with the unaided eye (objects that are not within the resolution range of the normal eye).
Microscopists explore the relationships between structures & properties for a very wide variety of materials ranging from soft to very hard, from inanimate materials to living organisms, in order to better understand it. Zachariaz Janssen 1585 Robert Hooks 1665
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Lecture 2 MICROSCOPY.pptx
170male reproductive systemmale reproductive system
xtestis is covered by three layers (from
outside to inwards):
̞visceral layer of tunica vaginalis:
̎it is lined by flat mesothelial cells.ons of seminiferous tubules lined by spermatogonia, primary and secondary
xThese tight junctions form the blood–testis
barrier.
xThe tight junction divides the intercellular
compartment between the Sertoli cells
into basal and luminal compartment.
xBasal compartment contains spermato
gonia and primary spermatocytes.
xLuminal compartment contains secondary
spermatocytes and spermatids (Fig. 19.5).
Functions of Sertoli Cells
xSertoli cells provide support and nutrition
to spermatogenic cells.
xThe bloodtestis barrier protects the
spermatogenic cells from the harmful
substances (antigens) of blood.
xThey phagocytose the residual bodies.
xSertoli cells secrete androgen-binding
protein (ABP), which concentrates the
testosterone.
xIn fetal testis, Sertoli cells produce anti
mullerian hormone, which inhibits the
development of mullerian duct.
xSertoli cells are nondividing cells, highly
resistant to infection, malnutrition, and
radiation.
xThese produce inhibin, which inhibits the
secretion of follicle-stimulating hormone
(FSH).
Interstitial Cells of Leydig
xThese are large polyhedral cells lying in
the connective tissue between seminif
erous tubules.
xThese are pale staining cells with eccen
tric nucleus and cytoplasm shows unique
needleshaped crystalline inclusion
(Reinke’s crystal).
spermatocytes, spermatids, and sperms are seen.
2.Sertoli cells are seen in between the spermatogenic cells.
3.Interstitial ces of Leydig are seen in between the seminiferous tubules.
xThey secrete testoster
̞tunica albuginea:
̎it is a thin layer of connective tissue
containing collagen, blood vessels,
and lymphatics.
̎along the posterior border, tunica
albuginea is thickened to form medi
astinum testis.
̎septa arising from the mediastinum
testis divide the substance of the
testis into 200 to 300 lobules.
̎each lobule contains one to four
seminiferous tubules.
̎seminiferous tubules contain coiled
part in the front and straight part
behind.
̎straight part enters the medi
astinum testis where it joins and
forms a network called as rete testis.
̎from the upper end of rete testis
12 to 14 efferent ductules arise and
enter the epididymis.
̞tunica vasculosa:
̎highly vascularized connective
tissue which covers the individual
lobule.
microscopic structure
oftestis
seminiferous tubule
xthere are 400 to 600 seminiferous tubules
in each testis.
xeach tubule is surrounded by a basal
lamina supported by connective tissue
which contains muscle-like myoid cells.
xcontraction of myoid cells helps to move
the spermatozoa along the tubule.
xeach seminiferous tubule is lined by
stratified seminiferous epithelium which
contains spermatogenic cells and sertoli
cells(figs. 19.2and19.3).
fig. 19.2diagram of testis (h&e pencil). h&e, hematoxylin and eosin. 3.Interstitia
Types of Light Microscopes used in Histological Studies.pptx
Light microscopes relies on glass lenses and visible light to magnify tissue samples. It was
invented in XVII century, and has been improved over the years, resulting in the powerful
modern light microscopes. As individual cellular structures are too small to be seen by the
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This document provides information about microscopy. It begins by defining microscopy and explaining that microscopes are used to view microorganisms that are too small to see with the naked eye. It then discusses the history and basic principles of microscopy, including magnification, resolution, and numerical aperture. The document outlines the key parts of the microscope and describes different types of microscopes in more detail, including light microscopes (brightfield, darkfield, fluorescence, phase contrast, UV) and electron microscopes (TEM, SEM). It provides examples of how each type of microscope is used.
Microscopy introduction
i am HAFIZ M WASEEM from mailsi vehari
BSc in science college Multan Pakistan
MSC university of education Lahore Pakistan
I love Pakistan and my teachers
Microscope ppt, by jitendra kumar pandey,medical micro,2nd yr, mgm medical co...
The document summarizes different types of microscopes used to study microorganisms. It discusses light microscopes like brightfield, darkfield and phase contrast microscopes. It also describes electron microscopes like transmission electron microscopes (TEM) and scanning electron microscopes (SEM) that use electron beams instead of light. TEM images internal structures by transmitting electrons through thin samples while SEM scans sample surfaces using secondary electrons. Sample preparation methods for both light and electron microscopy are also outlined.
Enhancing Vision
The document discusses telescopes and microscopes. It describes that telescopes use lenses or mirrors to magnify distant objects and make them appear closer. The two main types of telescopes are refracting and reflecting. Microscopes are used to view objects too small to be seen by the naked eye and the two primary types are optical and electron microscopes. Optical microscopes use lenses and light to magnify, while electron microscopes like transmission and scanning electron microscopes use focused electron beams instead of light.
Mshot microscopy products catalog 2020
The catalog contains all of Mshot products line, including optical microscope, LED fluorescence illuminator, microscope camera, thermal plate and other accessories.
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Types of Light Microscopes used in Histological Studies.pptx
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Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
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