This document discusses peptide synthesis methods, including solution phase peptide synthesis and solid phase peptide synthesis (SPPS). SPPS involves attaching the starting peptide to an inert resin bead and performing sequential amino acid couplings and deprotections while the growing peptide remains attached to the resin. This allows for easy purification after each step. Once the full peptide is synthesized, it is cleaved off the resin bead. SPPS allows for faster synthesis of longer peptides up to 70-100 amino acids in length compared to solution phase synthesis. Applications of synthetic peptides include vaccines, hormones, antibiotics, toxins, and enzyme inhibitors.
THE SOLID PHASE PEPTIDE SYNTHESIS IS SLIGHTLY DIFFRENT FROM PEPTIDE SYNTHESIS WHICH IS DISCUSSED HERE, ITS SYNTHESIS WITH STRUCTURE ANS BASICS ARE DISCUSSED WHICH WILL BE VERY USEFUL FOR READERS.
A presentation discussing amino acids, peptide bonds and peptide synthesis. The Merrifield Synthesis of Peptides is further discussed covering principle, methodology, isolation and purification, its advantages and disadvantages. A brief note on protecting groups is mentioned. A few practical applications of synthetic peptides are mentioned and discussed in brief as well.
CHEMISTRY OF PEPTIDES [M.PHARM, M.SC, BSC, B.PHARM]Shikha Popali
THE CHEMISTRY OF PEPTIDES THE DIFFICULT TO COLLECT DATA FOR READERS , THREFORE HERE WE HAVE COLLECTED ALL THE DATA AT A PLACE AND PROVIDED EASIER TO CHEMISTRIANS.
THE SOLID PHASE PEPTIDE SYNTHESIS IS SLIGHTLY DIFFRENT FROM PEPTIDE SYNTHESIS WHICH IS DISCUSSED HERE, ITS SYNTHESIS WITH STRUCTURE ANS BASICS ARE DISCUSSED WHICH WILL BE VERY USEFUL FOR READERS.
A presentation discussing amino acids, peptide bonds and peptide synthesis. The Merrifield Synthesis of Peptides is further discussed covering principle, methodology, isolation and purification, its advantages and disadvantages. A brief note on protecting groups is mentioned. A few practical applications of synthetic peptides are mentioned and discussed in brief as well.
CHEMISTRY OF PEPTIDES [M.PHARM, M.SC, BSC, B.PHARM]Shikha Popali
THE CHEMISTRY OF PEPTIDES THE DIFFICULT TO COLLECT DATA FOR READERS , THREFORE HERE WE HAVE COLLECTED ALL THE DATA AT A PLACE AND PROVIDED EASIER TO CHEMISTRIANS.
Chemistry of peptide (BPHARM,MPHARM,MSC,BSC)Shikha Popali
THE PRESENTATION DESCRIBING BOND FORMATION OF AMINO ACIDS AND PROTEINS AND COUPLING REAGENTS IN PEPTIDE SYNTHESIS FOLLOWED BY CARBODIMIDES, PHOSPHONIUM AND AMMONIUM SALTS.
MERITS OF MICROWAVE ASSISTED REACTIONS
DEMERITS OF MICROWAVE ASSISTED REACTIONS
MECHANISM OF MICROWAVE HEATING
EFFECTS OF SOLVENTS IN MICROWAVE ASSISTED SYNTHESIS
MICROWAVE VERSUS CONVENTIONAL SYNTHESIS
MICROWAVE INSTRUMENTATION
VARIOUS TYPES OF MICROWAVE ASSISTED ORGANIC REACTIONS
APPLICATIONS OF MICROWAVE ASSISTED REACTIONS
Prediction of the three dimensional structure of a given protein sequence i.e. target protein from the amino acid sequence of a homologous (template) protein for which an X-ray or NMR structure is available based on an alignment to one or more known protein structures
Energy minimization methods - Molecular ModelingChandni Pathak
Methods to minimize the energy of molecules during drug designing - Computational chemistry. According to the PCI syllabus, B.Pharm 8th Sem - Computer-Aided Drug Design (CADD).
An artificial enzyme is a synthetic organic molecule or ion that mimics one or more functions of an enzyme.
Molecules are designed and modified to achieve some desirable features of enzymes.
Protein engineering has been developed to design and synthesize molecules with the attributes of enzymes for non-natural reactions.
They have a molecular weight of less than 2000 Dalton.
They have the ability to stabilize at a higher temperature.
They are also known as synzymes or enzyme mimics.
This power point contain introduction, all synthesisStrecker synthesis
(Amido-malonate
Reductive amination of alpha keto acids) (Solid phase (Merrifield)
Solution phase) of amino and and peptides.
Chemistry of peptide (BPHARM,MPHARM,MSC,BSC)Shikha Popali
THE PRESENTATION DESCRIBING BOND FORMATION OF AMINO ACIDS AND PROTEINS AND COUPLING REAGENTS IN PEPTIDE SYNTHESIS FOLLOWED BY CARBODIMIDES, PHOSPHONIUM AND AMMONIUM SALTS.
MERITS OF MICROWAVE ASSISTED REACTIONS
DEMERITS OF MICROWAVE ASSISTED REACTIONS
MECHANISM OF MICROWAVE HEATING
EFFECTS OF SOLVENTS IN MICROWAVE ASSISTED SYNTHESIS
MICROWAVE VERSUS CONVENTIONAL SYNTHESIS
MICROWAVE INSTRUMENTATION
VARIOUS TYPES OF MICROWAVE ASSISTED ORGANIC REACTIONS
APPLICATIONS OF MICROWAVE ASSISTED REACTIONS
Prediction of the three dimensional structure of a given protein sequence i.e. target protein from the amino acid sequence of a homologous (template) protein for which an X-ray or NMR structure is available based on an alignment to one or more known protein structures
Energy minimization methods - Molecular ModelingChandni Pathak
Methods to minimize the energy of molecules during drug designing - Computational chemistry. According to the PCI syllabus, B.Pharm 8th Sem - Computer-Aided Drug Design (CADD).
An artificial enzyme is a synthetic organic molecule or ion that mimics one or more functions of an enzyme.
Molecules are designed and modified to achieve some desirable features of enzymes.
Protein engineering has been developed to design and synthesize molecules with the attributes of enzymes for non-natural reactions.
They have a molecular weight of less than 2000 Dalton.
They have the ability to stabilize at a higher temperature.
They are also known as synzymes or enzyme mimics.
This power point contain introduction, all synthesisStrecker synthesis
(Amido-malonate
Reductive amination of alpha keto acids) (Solid phase (Merrifield)
Solution phase) of amino and and peptides.
Nanoparticles are sub-nanosized colloidal structures composed of synthetic or semi synthetic polymers.
The drug is dissolved, entrapped, encapsulated or attached to a nanoparticle matrix.
Amino acid analysis and peptide mapping Likhith KLIKHITHK1
Amino Acid Analyzer is specifically configured system optimized for analysis of free amino acids.
PURPOSE:
Detection of presence of Amino acid in variety of biological samples, such as
extracellular and intracellular fluids
plant and animal tissues,
broths, and fruits
beverage juices
Detection of presence of hydrolyzed Amino acid, such as found in
protein, collagen, peptides, and processes foods.
Peptide mapping is an identity test for proteins, especially those
obtained by r-DNA technology. Peptide mapping is a comparative procedure because the information obtained, compared to a Reference Standard or Reference Material similarly treated, confirms the primary structure of the protein, is capable of detecting whether alterations in structure have occurred, and demonstrates process consistency and genetic stability. Peptide mapping refers to the identification of proteins using data from intact peptide masses. It is a powerful test that is capable of identifying single amino acid changes resulting from events such as errors in the reading of complementary DNA (cDNA) sequences or point mutations.
drug execipent compatibilty studies is of prime importance for the better formulation of the new drug and also for reducing cost by verfication of the data at the earlier atage.
this presentation will give the brief explanation of the goal, importance, dteps involve to studi the drug execient compatibility studies with different examples suitable accordiingly.
It is the presentation for Combinatorial Chemistry. this presentation should be helpful for B. Pharm students. It includes introduction, types, applications, advantages and disadvantages.
In this presentation I'm giving idea about the protecting group for the protection of amino acid and amino group... I'm trying my best to elaborate all the points and try to give all the structure which is required or related with the topic. In this presentation I discussed about the protecting group why they required, why amino acid required protecting group, method , structures , mechanism, application etc .
I mainly discussed about the carbamate and amide as Protecting group..... In carbamate we discuss about cbz and boc protecting group and in amide I discussed about acetamide formamide and so on .........
I believe that this presentation is very helpful for our batchmate colleagues and students of m.pharm ( chemistry) , M. Sc chemistry and bsc students .........
Thank you
It consists classification of polymerization techniques. What is bulk polymerization, how will the reaction proceed, and what are the advantages, disadvantages, and applications. Similarly, what is solution polymerization and how it will be carried out, what are the advantages, disadvantages, and applications behind it everything is explained in detail. Some of the related questions are also included for practice. All the contents taken from different websites and books are also mentioned.
Recovery of 4-Aminophenol from Aqueous Solution Using Different techniques IJARIIE JOURNAL
4-Aminophenol belong to a class of organic substances of particular environmental interest. Due to their
widespread discharge into environment and their toxicity to many living organisms, phenol and its amine derivates
are presently found on most priority lists of hazardous substances.
Different techniques to separate phenolic compounds from aqueous solution has been developed which includes
extraction, absorption, distillation , freeze crystallization ,etc. To separate a phenolic compound from effluent & to
recover it is an essential need for the industry today. This report presents experimental for aqueous solution of 4aminophenol
with
help
of
Distillation
and
freeze
crystallization
system
which
is
utilized
in
finding
%
recovery
of
4aminophenol.
Using freeze crystallization method we did not get any recovery of 4-aminophenol so freeze
crystallization method don’t work in recovery of 4-aminophenol from aqueous solution. then used distillation
technique we get 80% recovery of p-aminophenol from aqueous solution. Based on experimental data, we proposed
to design the batch distillation column.
A Novel Approach to Internal Standardization in LC/MS/MS Analysis; Sensitive ...MicroConstants
This presentation was prepared by Bruce Babson, Research Fellow at MicroConstants, Inc. in San Diego, California, for the CACO-PBS Mini-Symposium on Bioanalytical and Analytical Applications and Problem Investigation Case Studies. Bruce is one of twelve presenters at the August 10, 2012 event in Foster City, California.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
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Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
2. • More than 40 marketed peptides worldwide
• 270 peptides in clinical trials
• 400 peptides in advanced preclinical phases
Peptides as Drugs Today
The polymers of amino acids that are linked by peptide bonds. The
protein is a polypeptide.
Formation of a peptide bond by condensation
3. Before starting….
• Choose the C-terminal protecting group
• Choose the N-terminal protecting group
• Choose the coupling reagent
Mainly peptide synthesis are two types
1. Solution phase peptide synthesis
2. Solid phase peptide synthesis
7. Solution Phase Peptide Synthesis
H2N COOMe
BocNH COOH
BocNH C
O
H
N CH2 COOMe
H2N C
H
N CH2 COOMe
O
acid protectionamine protection
coupling
amine deprotection
BocNH COOH
amine protection
coupling
N
H
C
H
N CH2 COOMe
O
C
O
BocNH
8. The Merrifield Method
Synthesized a nonapeptide (bradykinin) in
1962 in 8 days in 68% yield.
Synthesized ribonuclease (124 amino acids)
in 1969.
369 reactions; 11,391 steps
Nobel Prize in chemistry: 1984
10. In solid-phase synthesis, the starting
material is bonded to an inert solid support
Reaction occurs at the interface between
the solid and the solution. Because the
starting material is bonded to the solid, any
product from the starting material remains
bonded as well.
Purification involves simply washing the
byproducts from the solid support.
11. • First step is to attach the starting
molecule to an inert solid.
– Typically inert polymers or resins are
used.
– These are commercially availableSince the molecule is attached to a solid,
any other chemicals added or products can
be removed by filtration.
After all reactions are done the product is
still attached to the insoluble bead.
Finally, the product is cleaved from the
bead and isolated.
12. Solid phase peptide synthesis (SPPS)
Resin
O
AA1HNFmoc
Fmoc
P1
Resin
O
AA1H2N
P1
O
AA2HNFmoc
P2
OH
O
AA2HNFmoc
P2
A
A
Resin
O
AA1NH
P1
O
AA2HNFmoc
P2
A
activation deblocking
coupling repeat steps
for each amino
acid in peptide,
then deblock,
deprotect,
cleave off resin
Resin
Fmoc
P2P1
AA2AA1
A
solid supportsolid support
fmoc protecting groupfmoc protecting group
protecting groupsprotecting groups
for side chainsfor side chains
1st and 2nd1st and 2nd
amino acidsamino acids
carbonyl activatingcarbonyl activating
groupgroup
15. Applications of
synthetic peptides
Immune peptides:
synthetic antigens;
vaccines
diagnostic tools
immunostimulator peptides;
muramyl dipeptide
tuftsin derivatives
Hormones:
oxytocin
vasopressin
insulin
somatostatin
GnRH
etc.
Neuropeptides:
substance P
cholecystokinin
neurotensin
Antibiotics:
tachikinin
gramicidine S
Toxins:
conotoxins
spider toxins
snake toxins
ionchanel blockers
Enzymes and
enzyme inhibitors:
Ribonuclease A
Carriers:
templates
miniproteins
Peptides
for structural studies:
turn mimicking cyclic peptides
Transporter peptides:
penetratin
oligoarginine
HIV-Tat protein
16. • time consuming;
• side chain protecting groups for
• Lys, Asp, Glu, (Cys);
• coupling: less than 90% conversion;
• purification after each steps;
• large scale;
• cheap.
• fast;
• side chain protecting groups for
• all functional groups
• coupling: over 99.5% conversion;
• purification at the end;
• rather small scale;
• expensive.
Solution phase peptide synthesis Solid phase peptide synthesis
(SPPS)
17. MAPS (Multiple Antigenic Peptide System)
MAPS is a method for producing high-titer
anti-peptide antibodies and synthetic peptide
vaccines. This system utilizes the α- and ε-
amino functional groups of lysine to form a
backbone to which multiple peptide chains
are attached. Depending on the number of
lysine tiers (2, 4, 8, etc.), different numbers of
peptide branches can be synthesized. Using
this new technology, our customers have
successfully produced high-titer antibodies.