Metagenomics is the study of microbial communities directly from environmental samples without isolating individual microbial strains. Next-generation sequencing allows researchers to sequence thousands of organisms in parallel from a sample. Metagenomic studies involve extracting DNA from a sample, fragmenting the DNA, and sequencing the fragments to determine the microbial diversity and genes present in the community. The Illumina sequencing workflow involves sample preparation through fragmentation and adaptor ligation, cluster generation by bridge amplification on a flow cell, sequencing by synthesis using fluorescently labeled nucleotides, and data analysis to assemble sequences and identify genes.