The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Centres of diversity – types of biodiversity – Centres of origin – Law of homologous series – centers of origin – types of centres of diversity – gene sanctuaries – genetic erosion – main reasons of genetic erosion – extinction – introgression – gene banks – types of gene banks
Centres of diversity – types of biodiversity – Centres of origin – Law of homologous series – centers of origin – types of centres of diversity – gene sanctuaries – genetic erosion – main reasons of genetic erosion – extinction – introgression – gene banks – types of gene banks
Floral biology of Garlic and Onion
Scientific name : Allium sativum
Family : Alliaceae
Chromosome number : 2n = 2x = 16
Garlic is a herbaceous annual for bulb and a biennial for seed production. Garlic is frost hardy bulbous perennial erect herbs of 30-100 cm in height with narrow flat leaves and bears small white flowers and bulbils. The under-ground stems are modified as bulbs.
Scientific name : Allium cepa
Family : Alliaceae
Chromosome number : 2n = 2x = 16
Habitat & Habit : The onion is a biennial, but can perennate by underground bulbs in favourable conditions.
Stem : The true stem of onion is much reduced in size and is hard, erect, short with condensed internodes and flat bottom. It is held underneath the soil. The bulbous structure found above the soil surface known as “pseudostem” is constituted by the accumulation of the tubular leaf sheaths. The short stem produces the slender primary root from the centre of the base.
After a definite period, the apex of the stem elongates upward and produces the inflorescence axis or seed stalk known as scape.
Floral biology of Garlic and Onion
Scientific name : Allium sativum
Family : Alliaceae
Chromosome number : 2n = 2x = 16
Garlic is a herbaceous annual for bulb and a biennial for seed production. Garlic is frost hardy bulbous perennial erect herbs of 30-100 cm in height with narrow flat leaves and bears small white flowers and bulbils. The under-ground stems are modified as bulbs.
Scientific name : Allium cepa
Family : Alliaceae
Chromosome number : 2n = 2x = 16
Habitat & Habit : The onion is a biennial, but can perennate by underground bulbs in favourable conditions.
Stem : The true stem of onion is much reduced in size and is hard, erect, short with condensed internodes and flat bottom. It is held underneath the soil. The bulbous structure found above the soil surface known as “pseudostem” is constituted by the accumulation of the tubular leaf sheaths. The short stem produces the slender primary root from the centre of the base.
After a definite period, the apex of the stem elongates upward and produces the inflorescence axis or seed stalk known as scape.
Manuel Ruiz' presentation in the framework of the expert consultation on the ...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Flor Rodriguez' presentation in the framework of the expert consultation on t...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
ESTE TRABAJO FUE ADAPTADO PARA LA MATERIA DE LAS TIC´S EN EL DESARROLLO DE COMPETENCIAS Y LA CULTURA INVESTIGATIVA
PARA EL CURSO DE NIVELACION DE LA MAESTRIA EN GERENCIA EDUCACIONAL DE LA UNIVERSIDAD PEDAGOGICA EXPERIMETAL LIBERTADOR NUCLEO ACADÉMICO CARABOBO
AUTORES: UNIVERSIDAD PEDAGOGICA EXPERIMETAL LIBERTADOR NUCLEO ACADÉMICO CARABOBO LICENCIADOS ORSON SERRANO Y MARIA A. CUELLO
TUTOR: DR. DOUGLAS LEAL
JULIO 2016
Andy Jarvis' presentation in the framework of the expert consultation on the ...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Out Crossing, Heterozygosis and Inbreeding with Environments Interaction in R...paperpublications3
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Out Crossing, Heterozygosis and Inbreeding with Environments Interaction in R...paperpublications3
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Knowledge engineering: from people to machines and back
Elsa Camadro's presentation in the framework of the expert consultation on the use of crop wild relatives for pre-breeding in potato
1. Reproductive behavior and germplasm classification
of wild potato populations: relevance in germplasm
conservation and breeding
Elsa L. Camadro
Laboratorio de Genética
Instituto Nacional de Tecnología Agropecuaria
Universidad Nacional de Mar del Plata
Consejo Nacional de Investigaciones Científicas y Técnicas
Argentina
2. Premises of the presentation
The knowledge of the reproductive biology of wild potatoes
and its consequences in the functioning of natural populations
is of utmost importance to understand the morphological and
molecular variability encountered in the group
The choice of genetic materials and methodological approches
used to classify wild germplasm has direct consequences in:
population sampling
ex situ conservation of germplasm (gene frequencies)
breeding
3. Plant classifications in the XXth and XXIth centuries
Artificial binomial system developed
by von Linneus in the XVIIIth
based in the use of “holotypes”
(single physical examples or illustrations of organisms known
to have been used when the species or lower-ranked taxon
were formally described)
with the more recent aid of biochemical and molecular tools
4. Species Concept
based on
reproductive relations morphological phenotypes
and morphological discontinuities (uni- and biparental organisms)
(biparental organisms)
Biological species Taxonomic species
The Biological species can coincide (or not) with the Taxonomic species
If the two uses of the term are not distinguished,
the confusion is perpetuated
5. Genus Solanum
~1,000-2,000 species
(herbs or shrubs)
≥110 ≤235 wild and
cultivated tuber-
bearing species
Section Petota (Tuberarium)
Subsection Potatoe (Hyperbasarthrum)
Spooner y Hijmans 2001
6. Species concept in subsection
Hyperbasarthrum (actually Potatoe)
Early XXth century
Narrow taxonomic unit, with little phenotypic variability
(Bitter, Juzepczuk, Bukasov)
Mid XXth century
Profound revision of tuber-bearing species due
to great phenotypic variability encountered
(Hawkes 1963)
Late XXth and early XXIth centuries
New revisions (Hawkes 1990; Spooner´s group)
7. Taxonomic revisions
(last 20 years)
Mainly based on:
Herbarium materials and samples of living plantas,
with more recent application of molecular tools to
accessions with previously assigned specific categories
No. of tuber-bearing species:
Hawkes (1990): 227
Spooner and Hijmans (2001): 203
Spooner and Salas (2006): 189
Spooner (2009): converging around 110
8. Main features of wild and cultivated potatoes
Form polyploid series
(2n=2x, 3x, 4x, 5x, 6x; x=12)
Can reproduce:
•sexually (by seeds)
•asexually (by stolons and tubers)
9. Posses one multiallelic S-locus conferring
gametophytic SI
diploids obligate outcrossers
polyploids -allogamous (can self-pollinate by
competitive S-alleles interaction)
-autogamous (self-pollinate)
S7 S1 S2
S4
1st Third
S1 S2 S1 S2
Self-compatible Self-incompatible
10. -continues-
Present scarce genome differentiation
All species share a common genome (A),
and four derived genomes (B, C, D, E)
Under genetic control, can produce:
•2n pollen and/or 2n eggs
Gametophytes with sporophytic chromosome numbers
•Haploids
Sporophytes with gametophytic chromosome numbers
In nature, are isolated by external and/or
internal hybridization barriers
The internal barriers are genetically controlled
and can be incomplete
Camadro et al. 2004
11. General Observations
The breeding system provides for:
maintenance of superior genotypes in stable environments
evolution of new genotypic combinations in changing
environments
overlapping of generations (parental, hybrids, BCs)
The morphological phenotypic differences among taxa
are not of great magnitude
Hybridization and gene flow within and between
ploidy levels and generations produce very complex
patterns of morphological variation
12. Potato accessions
Samples of natural populations ex situ conserved as:
original collections or multiplications of original collections
Purposes
Conservation of samples of natural variability of a species
and of genes for breeding and other applied purposes
P1 P2 P3
xxxx xx x x x x x x
xxxx xx x x x x x x
xxxx x x x x x x x
x x x x x x
Passport information for potato accessions:
Collection date, locality, latitude, longitude and altitude a.s.l.
13. Information unavaible or lacking
In situ sampling
No. and spatial distribution of sampled plants at collection site
No. harvested fruits or tubers/sampled plant
No. sampled plants/population
How the seed sample (accession) is formed
If seedlings are sampled, their spatial distribution
Are accessions representative samples of
the natural genetic variability at the sampling site?
Ex situ seed/tuber multiplication
Effective no. of parental plants
No. harvested fruits or tubers/plant
No. harvested seeds/fruit
How the seed sample (accession) is formed
Discarding unwanted contamination/crossing
Has genetic drift being avoided?
14. Current classification approaches are based on
the Taxonomic species concept
not taken into consideration:
Natural populations of sexual reproducing allogamous plants
are expected to exhibit morphological and genetic variation
(one plant=one genotype)
Morphological and genetic variation can occur within and
between populations of the same taxonomic species
Natural populations can consist of uni- and biparental plants,
with overlapping of generations
Breeding relations within and among spontaneous populations
provide for hybridization and introgression among ploidy levels
and generations, creating complex patterns of variation
17. Prevention of fertilization due to abnormalities in
Pollen tube growth
Pollen germination
Estigma
1/3
1st Third 1er Tercio
2do2/3
Tercio
3 3/3
er
Tercio
ovary
Gametophytic SI Cross Incompatibility
Bilateral (CI)
Unilateral or Bilateral
Camadro y Peloquin 1981
20. Inviability of embryo and/or endosperm in the
F1 hybrid seed due to abnormal embryo and/or
endosperm development/abortion
Hybrid sterility (principally male sterility)
Inviability or weakness of F2 and advanced
segregating generations
Camadro et al. 2004
21. Inviability of F1 hybrid seed
endosperm Genetic causes
(EBN)
Abortion
Endosperm abortion
embryo
Genetic causes
(cannot be circumvented)
22. 2x 1NBE cmm x 2x 1NBE cmm, 15 DDP 4x 2NBE acl x 2x 1NBE cmm, 9 DDP
2x 2NBE grl x 4x 4NBE grl, 9 DDP 4x 2NBE acl x 2x 2NBE grl, 11DDP
Masuelli y Camadro (1997)
24. Male sterility and cytological abnormalities
Interspecific artificial hybrids
Diploids: S. tuberosum (tbr) x S. chacoense (chc),
tbr x S.gourlayi (grl),
tbr x S. spegazzinii (spg)
(Santini et al. 2000)
Tetraploids: grl x tbr and tbr x grl
(Larrosa et al. 2005)
Spontaneous hybrids
grl-S. infundibuliforme
(Larrosa et al. 2005)
25. Species of hybrid origin
S. ruiz-lealli
(Raimondi et al. 2000; Marfil et al. 2009)
“Species”
S. okadae
(Camadro et al. 2007; Bottini et al. 2008)
S. chacoense
(Bottini et al. 2009; Larrosa et al. 2010;
Poulsen et al. 2011)
29. polen
Fertile and sterile pollen in spontaneous and artificial hybrids and
populations with specific category
Larrosa et al. 2010
30. Tetrad stage in spontaneous and artificial hybrids
and populations with specific category
Larrosa et al. 2010
31. P MI MII AI
MII AI AII MII
AII AII TI TII
Meiosis in spontaneous and artificial hybrids
and populations with specific category
Larrosa et al. 2010
32. Bolivia Argentina
a b
a
S. okadae (2x)
Accessions from Germplasm Potato Bank,
INTA, Argentina
Argentina Camadro et al. 2007
33. S. kurtzianum (2x)
OKA 872 OKA 5026 OKA 6003
OKA 6144 OKA 4505 OKA 6125
Accessions from Germplasm Potato Bank, INTA, Argentina
Bedogni & Camadro 2009
34. Evaluation of morphological characters
(quanti- and qualitative) and molecular markers (SSR)
Accessions from:
S. kurtzianum, Catamarca, La Rioja and
Mendoza
Sympatric “species”:
•S. chacoense (chc),
•S. spegazzinii (spg)
“outliers”
•S. oplocense (opl) y S. maglia (mag)
Genetic distances among sympatric accessions
with different specific statuts
was smaller than among allopatric accessions
with the same specific status
Bedogni & Camadro 2009
35. Accessions of S. kurtzianum (ktz) S. chacoense (chc), S.
spegazzinii (spg), S. maglia (mag) and S. oplocense (opl)
opl
C OKA 6147
C OL 4943
C OKA 6144
M SCl 4505
R OKA 6003
R OKA 6125
M ClM 872
C OKA 6141
C OKA 5026
M mag
R OKA 6113
C OKA 6107
0.00 0.22 0.45 0.67 0.90
SM Coefficient
= chc = ktz = spg
C=Catamarca, M=Mendoza, R=La Rioja
Nuclear SSR markers
Bedogni & Camadro 2009
36. UPGMA phenogram of accessions of S. kurtzianum (ktz),
S. chacoense (chc), S. spegazzinii (spg), S. maglia (mag) and
S. tuberosum ssp. tuberosum (tbr)
M ClM 872
M mag
M SCl 4505
R OKA 6125
C OKA 6144
R OKA 6003
R OKA 6113
tbr
C OKA 6147
C OKA 6107
C OKA 6141
C OL 4943
C OKA 5026
0.00 1.60 3.21 4.81 6.42
Euclidean Distance Coefficient
= chc = ktz = spg
C=Catamarca , M= Mendoza, R= La Rioja
Morphological Qualitative Traits
Bedogni & Camadro 2009
37. UPGMA phenogram of accesssions of S. kurtzianum (ktz),
S. chacoense (chc), S. spegazzinii (spg), S. maglia (mag) and
S. tuberosum ssp. tuberosum (tbr)
tbr
C OKA 6107
C OKA 6147
M mag
R OKA 6003
C OKA 5026
C OL 4943
M SCl 4505
C OKA 6144
R OKA 6113
R OKA 6125
M ClM 872
C OKA 6141
0.00 2.02 4.05 6.07 8.09
Euclidian Distance Coefficient
Morphological Quantitative Traits
Caracteres Morfológicos Cuantitativos
Bedogni & Camadro 2009
38. To fully exploit in breeding the genetic variability
of the wild potato germplasm
Screen various accessions (if available),
(particularly if the “species” has wide geographic or environmental distribution)
Take into consideration:
macro- and micro-environments of the originally sampled sites
type of original samples (botanical seed, seedlings, tubers)
Screen 15-25 plants/accession for allogamous “species”
(one plant= one genotype) and ≥10 plants for autogamous “species”
(there could be homozygosity for different gene combinations)
Use incomplete diallele crossing designs
(keeping tract of individual genotypic combinations. Breeding barriers
can be incomplete, hybrids are very frequent and there are
nuclear-cytoplasmic interactions)
Conclude with respect to a given accession, not a species
(do not extrapolate results).