This document summarizes the goal and objectives of pre-breeding work at the International Potato Center (CIP) focused on using crop wild relatives (CWR) to improve potato varieties. The goal is to introduce valuable genes from CWR and landraces into improved varieties to enhance sustainable farming. Objectives include identifying, combining, and documenting new traits from CWR and landraces for use in potato breeding programs. The document provides examples of traits and pre-breeding approaches used at CIP, such as introducing resistance to pests, diseases and abiotic stresses into advanced potato populations and varieties.
Andy Jarvis' presentation in the framework of the expert consultation on the ...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
NOVA PhD training course on pre-breeding, Nordic University Network (2012)Dag Endresen
Pre-breeding for sustainable plant production. Nova PhD course, January 2012 at Röstånga in Southern Sweden. Nova is a Nordic University Network.
Pre-breeding provides an important element in broadening the genetic diversity and introducing new and useful traits and properties to the food crops. New traits introduced in pre-breeding activities are not least important to meet the new challenges agriculture will face from the on-going climate change. The needed genetic diversity is often available outside of the genepool of cultivars and elite breeding lines. And sources of novel genetic diversity such as the primitive crops and even the wild relatives of the cultivated plants are expected to get increased focus when facing new challenges in agriculture.
The GBIF data portal provides information on in situ occurrences for many of the wild relatives to the cultivated plants that are not (yet) collected and accessioned by the ex situ seed genebank collections. The GBIF data portal will therefore provide a very valuable bridge between these data sources for genebank accessions and occurrence data sources outside of the genebank community. Occurrences from the GBIF data portal will assist in the identification of locations where potentially useful populations of crop wild relatives can be found. Ecological niche modeling provides a widely used approach for predicting species distributions and can be used for this purpose.
Recent work on predictive modeling to identify a link between useful crop traits and eco-geographic data associated with the source locations for germplasm may have particular value for pre-breeding efforts. The Focused Identification of Germplasm Strategy (FIGS) provides and approach for efficient identification of germplasm material with new and useful genetic diversity for a target trait property. Such predictive modeling approaches are of particular interest when performing pre-breeding because of the high costs related to working with this material. Cultivated plants are domesticated for properties and traits such as non-shattering seed behavior and more uniform harvest time that makes conducting agricultural experiments easier and less costly. Non-domesticated germplasm material and also the older cultivars and landraces have many agro-botanical traits that was moderated in modern cultivars to better suit agricultural practices and efficiency. Pre-breeding is largely about removing such undesired traits from the non-cultivated and less intensively domesticated material while maintaining potentially useful traits.
Nova PhD course home page:
http://www2.nova-university.org/chome/cpage.php?cnr=03-110404-412
https://sites.google.com/site/novaplantimprovementnetwork/home/phd-course-in-sweden-january-2012
Ewa Zimnoch-Guzowska's presentation in the framework of the expert consultati...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Bangladesh is situated in the subtropical region of the world and has enough opprtunities for commercial vegetable cultivation. Near about 65-70 vegetable crops are grown here. Besides domestic consumption Bangladesh is now exporting fresh vegetables in the European and Middle East countries. Therefore,utilizing breeding techniques a lot of more productive, disease resistant, good quality varieties of different vegetable crops can be developed here in Bangladesh. These new varieties can promote the Agro-Industry sector as well. Here brief descriptions of two important crops Tomato & Hot pepper which has the potetiality to provide support to the industry sector has been elaborated. AVRDC- The World Vegetable Centre has kindly supplied the germplasms and utilizing breeding techniques new tomato hybrid has been developed at BRAC while through adaptive trial one promising hot pepper hybrid has been identified in the BRAC Agricultural Research & Development Centre. #suggested link of my you tube video on research activities on eggplant at BARDC: https://www.youtube.com/watch?v=cZ3P5fTr7GU&t=1s
Andy Jarvis' presentation in the framework of the expert consultation on the ...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
NOVA PhD training course on pre-breeding, Nordic University Network (2012)Dag Endresen
Pre-breeding for sustainable plant production. Nova PhD course, January 2012 at Röstånga in Southern Sweden. Nova is a Nordic University Network.
Pre-breeding provides an important element in broadening the genetic diversity and introducing new and useful traits and properties to the food crops. New traits introduced in pre-breeding activities are not least important to meet the new challenges agriculture will face from the on-going climate change. The needed genetic diversity is often available outside of the genepool of cultivars and elite breeding lines. And sources of novel genetic diversity such as the primitive crops and even the wild relatives of the cultivated plants are expected to get increased focus when facing new challenges in agriculture.
The GBIF data portal provides information on in situ occurrences for many of the wild relatives to the cultivated plants that are not (yet) collected and accessioned by the ex situ seed genebank collections. The GBIF data portal will therefore provide a very valuable bridge between these data sources for genebank accessions and occurrence data sources outside of the genebank community. Occurrences from the GBIF data portal will assist in the identification of locations where potentially useful populations of crop wild relatives can be found. Ecological niche modeling provides a widely used approach for predicting species distributions and can be used for this purpose.
Recent work on predictive modeling to identify a link between useful crop traits and eco-geographic data associated with the source locations for germplasm may have particular value for pre-breeding efforts. The Focused Identification of Germplasm Strategy (FIGS) provides and approach for efficient identification of germplasm material with new and useful genetic diversity for a target trait property. Such predictive modeling approaches are of particular interest when performing pre-breeding because of the high costs related to working with this material. Cultivated plants are domesticated for properties and traits such as non-shattering seed behavior and more uniform harvest time that makes conducting agricultural experiments easier and less costly. Non-domesticated germplasm material and also the older cultivars and landraces have many agro-botanical traits that was moderated in modern cultivars to better suit agricultural practices and efficiency. Pre-breeding is largely about removing such undesired traits from the non-cultivated and less intensively domesticated material while maintaining potentially useful traits.
Nova PhD course home page:
http://www2.nova-university.org/chome/cpage.php?cnr=03-110404-412
https://sites.google.com/site/novaplantimprovementnetwork/home/phd-course-in-sweden-january-2012
Ewa Zimnoch-Guzowska's presentation in the framework of the expert consultati...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Bangladesh is situated in the subtropical region of the world and has enough opprtunities for commercial vegetable cultivation. Near about 65-70 vegetable crops are grown here. Besides domestic consumption Bangladesh is now exporting fresh vegetables in the European and Middle East countries. Therefore,utilizing breeding techniques a lot of more productive, disease resistant, good quality varieties of different vegetable crops can be developed here in Bangladesh. These new varieties can promote the Agro-Industry sector as well. Here brief descriptions of two important crops Tomato & Hot pepper which has the potetiality to provide support to the industry sector has been elaborated. AVRDC- The World Vegetable Centre has kindly supplied the germplasms and utilizing breeding techniques new tomato hybrid has been developed at BRAC while through adaptive trial one promising hot pepper hybrid has been identified in the BRAC Agricultural Research & Development Centre. #suggested link of my you tube video on research activities on eggplant at BARDC: https://www.youtube.com/watch?v=cZ3P5fTr7GU&t=1s
Sandra Knapp's presentation in the framework of the expert consultation on th...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Manuel Ruiz' presentation in the framework of the expert consultation on the ...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Shelley Jansky's and Reinhard Simon's talk on taxonomic and biogeographic pre...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Elsa Camadro's presentation in the framework of the expert consultation on th...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Flor Rodriguez' presentation in the framework of the expert consultation on t...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Arturo Brenes' presentation in the framework of the expert consultation on th...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Alberto Salas' presentation in the framework of the expert consultation on th...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Craig Yencho's presentation in the framework of the expert consultation on th...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Shelley Jansky's presentation in the framework of the expert consultation on ...cwr_use
The expert consultations on the use of crop wild relatives for pre-breeding in potato was a workshops organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Tatjana Gavrilenko's presentation in the framework of the expert consultation...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
Sandra Knapp's presentation in the framework of the expert consultation on th...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Manuel Ruiz' presentation in the framework of the expert consultation on the ...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Shelley Jansky's and Reinhard Simon's talk on taxonomic and biogeographic pre...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Elsa Camadro's presentation in the framework of the expert consultation on th...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Flor Rodriguez' presentation in the framework of the expert consultation on t...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Arturo Brenes' presentation in the framework of the expert consultation on th...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Alberto Salas' presentation in the framework of the expert consultation on th...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Craig Yencho's presentation in the framework of the expert consultation on th...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Shelley Jansky's presentation in the framework of the expert consultation on ...cwr_use
The expert consultations on the use of crop wild relatives for pre-breeding in potato was a workshops organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
Tatjana Gavrilenko's presentation in the framework of the expert consultation...cwr_use
The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
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Neuro-symbolic (NeSy) AI is on the rise. However, simply machine learning on just any symbolic structure is not sufficient to really harvest the gains of NeSy. These will only be gained when the symbolic structures have an actual semantics. I give an operational definition of semantics as “predictable inference”.
All of this illustrated with link prediction over knowledge graphs, but the argument is general.
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
Speakers:
👨🏫 Andras Palfi, Senior Product Manager, UiPath
👩🏫 Lenka Dulovicova, Product Program Manager, UiPath
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
Encryption in Microsoft 365 - ExpertsLive Netherlands 2024Albert Hoitingh
In this session I delve into the encryption technology used in Microsoft 365 and Microsoft Purview. Including the concepts of Customer Key and Double Key Encryption.
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Merideth Bonierbale's presentation in the framework of the expert consultation on the use of crop wild relatives for pre-breeding in potato
1. Overview of pre-breeding w/
focus on the use of CWR in
potato improvement at CIP
M. Bonierbale, E. Mihovilovich, B. Ordoñez
Global Crop Diversity Trust CWR Consultancy: Potato PreBreeding
2/22/2012, Lima, Peru
2. Pre-breeding Goal
Assure the flow of valuable genes and diversity
from CWR and landrace germplasm into
productive, improved varieties that enhance
sustainable and profitable farming systems.
Objectives
– Identify, upgrade, combine and document new sources of
needed traits for their incorporation into useful support
population and stocks.
– Support the continued utility of CIP’s broad-based tropically-
adapted potato populations for variety selection and potato
improvement.
3. General Pre-breeding Targets
& Cases for Potato at CIP
A. Traits “missing” from advanced Ø Pests, PVY, PVX, BW
populations and adapted varieties. resistance
B. Higher levels and more highly
heritable sources of needed traits. Ø PLRV resistance
C. Complementary sources to build
durable resistance to variable Ø Late blight resistance
pathogens.
D. Horizon scanning: Emerging Ø Heat, salinity and
Constraints; Climate Change. drought tolerance
E. Opportunities for new/added-value Ø Nutritional value
traits not previously attended by (micronutrient density)
breeding programs.
5. Progress and prospects of Potato
Improvement by sources and traits
(CIP at a glance)
Before CIP's
40 years' CIP History Future
foundation
80 85 90 95 00 05 10
Late Blight dms ,
A B P T
Potato Cyst Nematode
(Globodera pallida)
Frost
PVY, PVX acl,
s to
PLRV
Potato Tuber Moth
Root Knot Nematode
Early Blight
Bacterial Wilt
Drought
Bioavailable Micronutrients
7. Assets: Raw and Improved Germplasm
Adapted to Tropical Agro-ecologies
Lowland
phu
Tbr Adg tropics and
Neo dms Virus Resistant
tbr (LTVR)
Population
dms
1.2
Lesion radius (mm)
y = a+bx
Tbr 1.0
blb phu
b=LGR= 5.7
Highland
0.8
0.6
acl Neo
Adg tbr
0.4
0.2
y = a+bx
b=LGR= 3.6
tropics
0.0
3 5 7
Late Blight
Days after inoculation
Resistant (B3)
Population
8. Sustainability Population Improvement
Progenitors Incorporation Progenitors
New Cycles
Nobilisation and Genetic studies:
Introgression of • Parental value
Evaluation • Heritability
new diversity
• Variability
Support Yield, Stability
Populaciones Advanced
populations
Adaptation
Elite clones
10. Pre-breeding forBW + RKN Resistance
1977 -1995
BW, LB
dms × tbr (Mex germ:Atzimba) S.
sparsipilum
x S.
chacoense
tbr (earliness),
phu (CCC)x
tbr (Katahdin)
(University
of
Wisconsin)
Katahdin,
Atlantic,
DTO-‐28 E S.
microdontum
(
CIP,
1977)
S.
phureja BW
2x
BR,
MS,
PSP,
PSW Katahdin,
Atlantic,
DTO-‐28 E, HT
HT Stn,
phu.
Gon
tbr (heat
tolerance), (Long
adapted
Group
V
(2x)
Group
I
X
M.
tropical,
Serrana
germplasm)
X MBN
(BW
+
RKN)
CGN-‐69.1 (1977) CGN-‐69.1
PI/PS
bulk
X (
CIP,
1977)
(NCSU) (1980)
Group
II
‘Nobilisation”
Group
III (1979)
BW (1979-‐80)
4x
x
2x
Group
VI
(2x)
X MBN
+
TUBEROSA
Group
IV (1983)
(1980)
raph
,
chc
(AVRDC-‐1287.19)
Group
VII CIP
BW TD
(4x)
(1985) X LTVR
population E, HT, V
(4x)
4x
population
(1995)
Highly
heterozygote
Four
sources
of
BW
resistance
+
virus
resistance
12. Products of Collaborative BW Resistance
PreBreeding Program
391930.1 394904.17
BW, LB, PVX,
BW,
High
high concentration concentration
Zinc and Vit C Iron and Zinc
394908.13
395443.103
BW, PVX,
high concentration BW, LB –HR, PVX,
Zinc high concentration
Zinc and Vit C
395446.1
BW, PVX, PVY,
High concentration Iron and Zinc
13. Highly Heterozygous Nematode Resistant Variety
2x Breeding follow by 2x-4x Hybridization
vrn 2488-A (C.P.C. collection)
82M124.27
HT Bulk - stn-phu (NCSU)
84.28.42 stn-phu (NCSU)
378908.43
381343.40 (MBN) S. spl
Bulk Kura CIP 760147.7
85.27.13 (2x) (Pi 1230502)
(CIP PTL 80M (virus)
591099.13) MI-7.10 stn-phu (NCSU)
BW
84.194.11 S. spl
(MBN)
Nemared CIP 760147.7
MI-49.10 (Pi 1230502)
387559.3
released in
1994
AQUILA [tbr x (tbr x phu)]
LT-1
KATAHDIN (tbr)
LT-8 (4x)
PVXY Bk. (adg, neo-tbr)
14. Strategic Broadening of the Late Blight
Resistance of Advanced PopB3
1.2
Lesion radius (mm)
y = a+bx
1.0
b=LGR= 5.7
B3 0.8
0.6
Population 0.4
y = a+bx
0.2 b=LGR= 3.6
S bulbocastanum S. .tuberosum 0.0
S phureja 2x=48 3 5 7
2x=24
S acaule Days after inoculation
2x=24
4x=48 S. demissum
2x=48
S. tuberosum 25
S. andigena
Number of families
2x=24 2x=48 20
15
10
B3C3
5
B3C0
0
AUDPC (Family Means)
16. 2. 2. Search for Novel Resistance Sources:
Evaluate Putative Novel Resistance Sources
Piurana and Tuberosa Groups
Field and Detached leaf
Assays
S. chiquidenum Comas. Oxapampa
2000-2004
S. paucissectum
S. piurae
S. cajamarquense
17. 3. Pre-breeding with Putative Novel Resistance Sources
Embryo Rescue
Technique
Prezygotic barriers Field Testing & Selection
4x × 2x (B3 clones x ER)
Field testing
2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013
Selection:
3rd
resistance +
2nd agronomic +
Project
start Crossing Blocks reproductive
1st Selected
wild 2x
Pre Breeding
populations traits.
Dihaplod Native
2x selections
PF A B C D PM
wild wild x =
x
A B C D
Diallel wild x wild cross
Hybridity test
Field Testing and selection
19. Comparative Gene Expression between
B3 and S. cajamarquense under LB Challenge
Study# 83 at
Solanaceae
Gene expression
database:
http://www.tigr.org/tdb/potato/
SGED_index2.shtml
Design
:
advanced B3 S. cajamarquense
clone (cjm)
Inoculation with isolates
POX067 and PE B3
274
Sample collection at 72h and 96h after 30
inoculation cjm
329
Hybridization to the TIGR10K potato array
20. Lesson learned
• Interspecific • Innovative approaches
reproductive barriers (Sli?)
• Lack of fertile • Selection &
recipients w/ good
tuber appearance consolidation of pool of
compatible recipients
• Dilution of resistance • Sufficient
during breeding recombination; Monitor
process w/ molecular tools.
• Poorly-suited genetic • Attention to adaptation
background of recipient germplasm
21. Lesson Learned
• Variability of • Use and monitor
pathogens complementary sources
• Accumulation of • Monitor glycoalkaloids
glycoalkaloids accumulation during
breeding process
• Large populations
and cumbersome • Rapid and affordable
screening methods screening methods
• Limited, short term • Develop and debate
project funding business models
• Capitalize products per
se? Courses?
22. Recomendations
• Develop mechanism to sustain funding for
maintenance of most-valuable prebreeding
populations and stocks
• Document, communicate and create
demand for products for use in house and
across institutions
• Develop Community of Practice for potato
pre-breeding
23. More Pre-breeding Products
112 rare LB
resistant
hybrids with 2n
pollen and
good
agronomic
performance
(ER)