1. A motor unit includes a motor neuron and all of the muscle fibers it controls. When the motor neuron fires an action potential, it causes the release of acetylcholine at the neuromuscular junction, which then triggers an action potential in the muscle fiber. 2. The muscle action potential spreads throughout the fiber via t-tubules, causing the release of calcium from the sarcoplasmic reticulum. 3. The calcium binds to troponin, exposing actin binding sites for myosin, leading to cross-bridge cycling and muscle contraction.