This document summarizes an experiment on the effects of salinity on freshwater fishes. The experiment involved exposing freshwater fish to three different salinity levels (0%, 5%, and 15%) over seven days and observing changes. At 15% salinity, fish exhibited low energy, rigidity, loss of scales, and secretion from the body. Some fish died within a few days at 15% salinity. Physiological changes and deaths increased with higher salinity levels as freshwater fish lack mechanisms to tolerate saltwater. The results indicate that changes in salinity beyond about 9% can negatively impact or kill freshwater fish due to osmotic stress on their bodies.