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‫الرحيم‬ ‫الرحمن‬ ‫هللا‬ ‫بسم‬
Profundización en Química Analítica
Quantum Dots as pH Probes for Study of the Enzyme Kinetics
Ala’ Salem Alnaimat
1
Outlines: Introduction
- What is Quantum Dots (QDs)?
- Properties of QDs
- Applications of QDs
 Objective
 Advantages & Disadvantages
 Methodology
 Results
 conclusions
2
Introduction
What is Quantum Dots (QDs)?
QDs are luminescent semiconductor nanocrystals of an approximately 2–100 nm
( 10-50 atoms) in diameter.
Properties of QDs:
1- high brightness due to the extinction coefficient
2- broad absorption characteristics and a narrow band width in emission spectra.
3- Longer fluorescence lifetime ranging from 10 - 40 ns compared to the organic dyes.
The Quantum Dot
3
Applications of QDs:
Computing : increase the performance
and storage of computers
Biology: Cell labelling, cancer therapy, and
lymphocyte immunology
Light emitting devices (LOD): displays industry
Photovoltaic devices: increase the efficiency and reduce
the cost of today's typical silicon Photovoltaic cells.
Security Tags: can be detected using the night-vision goggles
4
Advantages & Disadvantages of QDs
Advantages: water soluble substances. Compared with the
traditional analytical methods based organic dyes using QDs
as pH probes was found to be a convenient, rapid and specific
method for the kinetics studies.
Disadvantages: QDs may be toxic according to its composition
which includes some heavy metals, and also the degradation of
QDs is still unknown specially inside the living organism.
5
Objective
QDs were used as pH probe for a reaction kinetic study of the hydrolysis
of glycidyl butyrate catalyzed by porcine pancreatic lipase (PPL)
6
Methodology
Glycidyl butyrate solution
ZnS QDs solution
Phosphate buffer saline
((PBS) (PPL) Enzyme solution
Incubation at 25˚C
for 1 min.
7
Results
The fluorescence intensity of ZnS QDs linearly decreases with time increasing, which
indicates that QDs can be successfully used as pH probes for enzyme reaction kinetic
study.Time ( a-j): 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 min
8
The effect of The emission spectra
of ZnS QDs to different additives.
(a) Ultra pure water; (b) methanol;
(c) PPL; (d) glycidyl butyrate;
(e) glycidol.on the fluorescence
intensity of QDs is investigated
No significant influence of
all these materials on the
fluorescence intensity of QDs
is observed.
9
conclusions
• The proposed method was found to improve stability,
sensitivity and a monitoring range for determination proton
concentration as compared to the methods based on the organic
dyes.
References:
• Dudu Wua, Zhi Chenb “ZnS quantum dots as pH probes for study of enzyme
reaction kinetics” Enzyme and Microbial Technology 51 (2012) 47– 52.
• Tan Jun, Wang Bochu, Zhu Liancai “ Quantum Dots: A novel tool to discovery”
Pakistan Journal of Biological Science. 9 (5) 917- 922, 2006
10
Muchas Gracias ….
11

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Quantum dots

  • 1. ‫الرحيم‬ ‫الرحمن‬ ‫هللا‬ ‫بسم‬ Profundización en Química Analítica Quantum Dots as pH Probes for Study of the Enzyme Kinetics Ala’ Salem Alnaimat 1
  • 2. Outlines: Introduction - What is Quantum Dots (QDs)? - Properties of QDs - Applications of QDs  Objective  Advantages & Disadvantages  Methodology  Results  conclusions 2
  • 3. Introduction What is Quantum Dots (QDs)? QDs are luminescent semiconductor nanocrystals of an approximately 2–100 nm ( 10-50 atoms) in diameter. Properties of QDs: 1- high brightness due to the extinction coefficient 2- broad absorption characteristics and a narrow band width in emission spectra. 3- Longer fluorescence lifetime ranging from 10 - 40 ns compared to the organic dyes. The Quantum Dot 3
  • 4. Applications of QDs: Computing : increase the performance and storage of computers Biology: Cell labelling, cancer therapy, and lymphocyte immunology Light emitting devices (LOD): displays industry Photovoltaic devices: increase the efficiency and reduce the cost of today's typical silicon Photovoltaic cells. Security Tags: can be detected using the night-vision goggles 4
  • 5. Advantages & Disadvantages of QDs Advantages: water soluble substances. Compared with the traditional analytical methods based organic dyes using QDs as pH probes was found to be a convenient, rapid and specific method for the kinetics studies. Disadvantages: QDs may be toxic according to its composition which includes some heavy metals, and also the degradation of QDs is still unknown specially inside the living organism. 5
  • 6. Objective QDs were used as pH probe for a reaction kinetic study of the hydrolysis of glycidyl butyrate catalyzed by porcine pancreatic lipase (PPL) 6
  • 7. Methodology Glycidyl butyrate solution ZnS QDs solution Phosphate buffer saline ((PBS) (PPL) Enzyme solution Incubation at 25˚C for 1 min. 7
  • 8. Results The fluorescence intensity of ZnS QDs linearly decreases with time increasing, which indicates that QDs can be successfully used as pH probes for enzyme reaction kinetic study.Time ( a-j): 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 min 8
  • 9. The effect of The emission spectra of ZnS QDs to different additives. (a) Ultra pure water; (b) methanol; (c) PPL; (d) glycidyl butyrate; (e) glycidol.on the fluorescence intensity of QDs is investigated No significant influence of all these materials on the fluorescence intensity of QDs is observed. 9
  • 10. conclusions • The proposed method was found to improve stability, sensitivity and a monitoring range for determination proton concentration as compared to the methods based on the organic dyes. References: • Dudu Wua, Zhi Chenb “ZnS quantum dots as pH probes for study of enzyme reaction kinetics” Enzyme and Microbial Technology 51 (2012) 47– 52. • Tan Jun, Wang Bochu, Zhu Liancai “ Quantum Dots: A novel tool to discovery” Pakistan Journal of Biological Science. 9 (5) 917- 922, 2006 10