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Scientific classification
Kingdom: Bacteria
Phylum: Proteobacteria
Class: Gamma Proteobacteria
Order: Pseudomonadales
Family: Pseudomonadaceae
Genus: Pseudomonas
Species: P. aeruginosa
Binomial name
Pseudomonas aeruginosa
Pseudomonas Infections
Survive where most
organisms cannot;
e.g., “oil-eating”
bacteria are
Pseudomonas.
(Slime layer)
Opportunistic pathogen
Flagella
Pseudomonas aeruginosa
Structure
1.Exotoxin A
 Similar in structure to Diptheria toxin
 Causes Dermatonecrosis in burn
wounds, corneal damage in ocular
infections, and tissue damage in
chronic pulmonary infections.
 Also this toxin is immunosuppressive
Heat labile hemolysin
Breaks down lipids and lecithin causing
tissue destruction
Stimulates inflammatory response
2.Phospholipase C
3.Elastase and Alkaline Protease
• Destruction of elastin-containing tissues
(blood vessels, lung tissue, skin), collagen,
immunoglobulins, and complement factors
• Can produce hemorrhagic lesions (ecthyma
gangrenosum)
• Inactivation of interferon
Clinical Disease
Pulmonary Infections
Burn Wound Infections and other skin and soft tissue
infections (life threatening)
UTI’s (especially catheterized)
External Otitis (malignant OE, swimmer’s ear)
Eye Infections and corneal ulceration via contaminated
contact lens cleaning fluids
Pseudomonal Endocarditis
Grow on most laboratory media
Blood agar
MacConkey’s agar
Minimal nutritional reqts.;
Many organic compounds used
as C and N sources, but only a
few carbohydrates by oxidative
metabolism
tolerate broad temperature
spectrum
its ability to grow at 42° C.
Isolation and identification
Motility of organism
Colony characteristic:
 Characteristic grape-like odor and blue-green pus & colon
 Lactose negative on MacConkey’s agar
 Some strains produce diffusible pigments:
Pyocyanin (blue);
Fluorescein (yellow);
Pyorubin (red)
 Fluorescence is helpful in early identification
CharacteristicsCharacteristics
Biochemical test:
inability to ferment lactose,
oxidase & catalase positive
Nitrate reduction negative
Catalase test
THANK YOU

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Pseudomonas fi

  • 1.
  • 2. Scientific classification Kingdom: Bacteria Phylum: Proteobacteria Class: Gamma Proteobacteria Order: Pseudomonadales Family: Pseudomonadaceae Genus: Pseudomonas Species: P. aeruginosa Binomial name Pseudomonas aeruginosa
  • 3. Pseudomonas Infections Survive where most organisms cannot; e.g., “oil-eating” bacteria are Pseudomonas. (Slime layer) Opportunistic pathogen Flagella
  • 6. 1.Exotoxin A  Similar in structure to Diptheria toxin  Causes Dermatonecrosis in burn wounds, corneal damage in ocular infections, and tissue damage in chronic pulmonary infections.  Also this toxin is immunosuppressive Heat labile hemolysin Breaks down lipids and lecithin causing tissue destruction Stimulates inflammatory response 2.Phospholipase C
  • 7. 3.Elastase and Alkaline Protease • Destruction of elastin-containing tissues (blood vessels, lung tissue, skin), collagen, immunoglobulins, and complement factors • Can produce hemorrhagic lesions (ecthyma gangrenosum) • Inactivation of interferon
  • 8. Clinical Disease Pulmonary Infections Burn Wound Infections and other skin and soft tissue infections (life threatening) UTI’s (especially catheterized) External Otitis (malignant OE, swimmer’s ear) Eye Infections and corneal ulceration via contaminated contact lens cleaning fluids Pseudomonal Endocarditis
  • 9.
  • 10. Grow on most laboratory media Blood agar MacConkey’s agar Minimal nutritional reqts.; Many organic compounds used as C and N sources, but only a few carbohydrates by oxidative metabolism tolerate broad temperature spectrum its ability to grow at 42° C. Isolation and identification
  • 12. Colony characteristic:  Characteristic grape-like odor and blue-green pus & colon  Lactose negative on MacConkey’s agar  Some strains produce diffusible pigments: Pyocyanin (blue); Fluorescein (yellow); Pyorubin (red)  Fluorescence is helpful in early identification CharacteristicsCharacteristics Biochemical test: inability to ferment lactose, oxidase & catalase positive Nitrate reduction negative Catalase test