The document discusses protein coronas that form around nanoparticles when introduced into biological fluids and how they impact physiological response. It summarizes that nanoparticles interact with proteins to form complexes with unique identities compared to the original nanoparticle. These complexes determine responses like uptake, circulation and toxicity. The document then examines several studies that show how nanoparticle properties like size and surface chemistry influence protein adsorption and subsequently impact biological response. It also reviews techniques for characterizing and experimentally investigating protein coronas and their effects.
Surface Plasmon Resonance (SPR) and its ApplicationDr. Barkha Gupta
DR. BARKHA GUPTA
ASSISTANT PROFESSOR (VETERINARY BIOCHEMISTRY)
DEPARTMENT OF VETERINARY PHYSIOLOGY AND BIOCHEMISTRY
POST GRADUATE INSTITUTE OF VETERINARY EDUCATION AND RESEARCH (PGIVER), JAIPUR RAJASTHAN UNIVERSITY OF VETERINARY AND ANIMAL SCIENCES (RAJUVAS), BIKANER
YouTube Channel: Barkha’s Vet Sphere
DNA Nanotechnology: Concept and its Applications
DNA Nanotechnology # Various 2 and 3 dimensional shapes of DNA nanotechnology # DNA Origami # with their application and Future scope
Surface Plasmon Resonance (SPR) and its ApplicationDr. Barkha Gupta
DR. BARKHA GUPTA
ASSISTANT PROFESSOR (VETERINARY BIOCHEMISTRY)
DEPARTMENT OF VETERINARY PHYSIOLOGY AND BIOCHEMISTRY
POST GRADUATE INSTITUTE OF VETERINARY EDUCATION AND RESEARCH (PGIVER), JAIPUR RAJASTHAN UNIVERSITY OF VETERINARY AND ANIMAL SCIENCES (RAJUVAS), BIKANER
YouTube Channel: Barkha’s Vet Sphere
DNA Nanotechnology: Concept and its Applications
DNA Nanotechnology # Various 2 and 3 dimensional shapes of DNA nanotechnology # DNA Origami # with their application and Future scope
introduction to Nanobiotechnology
what is nanotechnology
bionanotechnology
classical biotechnology industrial production using biological system
modern biotechnology from industrial processes to noval therapeutics
modern biotechnology immunological enzymatic and neucleic acid based technology
Dna based technology
self assembly and supramolecular chemistry
formation of ordered structure at nano scale
Nanotechnology is the manipulation of matter on atomic and molecular level (extremely small things).
It involves in the ability to see and control the individual atoms and molecules.
It has ability to design systems with defined structure and function on the nanometer scale.
Deals with the interdisciplinary areas :
Biology, Physics, Chemistry, Material science, Electronics,
Chemical Engineering, Information technology
The size of the thing in nano scale is measured by nanometer (nm).
1nm = 10−9
Nanomaterials in biomedical applicationsumeet sharma
An introduction to emerging technology in medicinal science, "nanodrugs" a fruitful combination of nano-science and medical science. In this presentation, use of nano shells for delivery of drugs to targeted cancer cells has been explained. along with In Vivo and In Vitro studies on use of nanomaterials for biomedical application. For any information please feel free to contact me or refer to the references.
Introduction
Definition
History
Advantages of nanobiotechnology
Applications of nanobiotechnology
Drawback of nanobiotechnology
New features in the nanobiotechnology
Conclusion
References
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
Protein based nanostructures for biomedical applications karoline Enoch
Proteins are kind of natural molecules that show unique
functionalities and properties in biological materials and
manufacturing feld. Tere are numerous nanomaterials
which are derived from protein, albumin, and gelatin. Tese
nanoparticles have promising properties like biodegradability, nonantigenicity, metabolizable, surface modifer, greater
stability during in vivo during storage, and being relatively
easy to prepare and monitor the size of the particles.
These particles have the ability to attach covalently with
drug and ligand
Antibacterial agents are very important in the textile industry, water disinfection, medicine, and food packaging. Organic compounds used for disinfection have some disadvantages, including toxicity to the human body; therefore, the interest in inorganic disinfectants such as metal oxide nanoparticles (NPs) is increasing. This review focuses on the Preparation and their potential with good antimicrobial activity of Ag-NPs and Se-NPs against biofilm forming S. aureus. Such improved antibacterial agents locally destroy bacteria, without being toxic to the surrounding tissue. We also provide an overview of opportunities and risks of using NPs as antibacterial agents. In particular, we discuss the role of Ag-NPs and Se-NPs materials. Several manufactured nanoparticlesparticles with one dimension less than 100 nm are increasingly used in consumer products. At nano size range, the properties of materials differ substantially from bulk materials of the same composition, mostly due to the increased specific surface area and reactivity, which may lead to increased bioavailability and toxicity. Thus, for the assessment of sustainability of nanotechnologies, methods of manufacturing Nanoparticles, properties have to be studied.
The formation of nanoparticle and physiochemical parameters such as pH, monomer concentration, ionic strength as well as surface charge, particle size and molecular weight are important for drug delivery. Further, these nanoparticles have the capability to reverse
multidrug resistance a major problem in chemotherapy. Well-established therapies commonly employed in cancer treatment include surgery, Chemotherapy, immunotherapy, and
radiotherapy. The silver nanoparticles might be involved in neutralizing these adhesive substances, thus preventing biofilm formation. Selenium is also one of essential trace elements in the human body and has great importance in nourishment and medicine. Medicaldiagnostic field also developed to use the selenium nanoparticles and also studies on the increase efficiency of glutathione peroxidase and thioredosin reductase.
introduction to Nanobiotechnology
what is nanotechnology
bionanotechnology
classical biotechnology industrial production using biological system
modern biotechnology from industrial processes to noval therapeutics
modern biotechnology immunological enzymatic and neucleic acid based technology
Dna based technology
self assembly and supramolecular chemistry
formation of ordered structure at nano scale
Nanotechnology is the manipulation of matter on atomic and molecular level (extremely small things).
It involves in the ability to see and control the individual atoms and molecules.
It has ability to design systems with defined structure and function on the nanometer scale.
Deals with the interdisciplinary areas :
Biology, Physics, Chemistry, Material science, Electronics,
Chemical Engineering, Information technology
The size of the thing in nano scale is measured by nanometer (nm).
1nm = 10−9
Nanomaterials in biomedical applicationsumeet sharma
An introduction to emerging technology in medicinal science, "nanodrugs" a fruitful combination of nano-science and medical science. In this presentation, use of nano shells for delivery of drugs to targeted cancer cells has been explained. along with In Vivo and In Vitro studies on use of nanomaterials for biomedical application. For any information please feel free to contact me or refer to the references.
Introduction
Definition
History
Advantages of nanobiotechnology
Applications of nanobiotechnology
Drawback of nanobiotechnology
New features in the nanobiotechnology
Conclusion
References
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
Protein based nanostructures for biomedical applications karoline Enoch
Proteins are kind of natural molecules that show unique
functionalities and properties in biological materials and
manufacturing feld. Tere are numerous nanomaterials
which are derived from protein, albumin, and gelatin. Tese
nanoparticles have promising properties like biodegradability, nonantigenicity, metabolizable, surface modifer, greater
stability during in vivo during storage, and being relatively
easy to prepare and monitor the size of the particles.
These particles have the ability to attach covalently with
drug and ligand
Antibacterial agents are very important in the textile industry, water disinfection, medicine, and food packaging. Organic compounds used for disinfection have some disadvantages, including toxicity to the human body; therefore, the interest in inorganic disinfectants such as metal oxide nanoparticles (NPs) is increasing. This review focuses on the Preparation and their potential with good antimicrobial activity of Ag-NPs and Se-NPs against biofilm forming S. aureus. Such improved antibacterial agents locally destroy bacteria, without being toxic to the surrounding tissue. We also provide an overview of opportunities and risks of using NPs as antibacterial agents. In particular, we discuss the role of Ag-NPs and Se-NPs materials. Several manufactured nanoparticlesparticles with one dimension less than 100 nm are increasingly used in consumer products. At nano size range, the properties of materials differ substantially from bulk materials of the same composition, mostly due to the increased specific surface area and reactivity, which may lead to increased bioavailability and toxicity. Thus, for the assessment of sustainability of nanotechnologies, methods of manufacturing Nanoparticles, properties have to be studied.
The formation of nanoparticle and physiochemical parameters such as pH, monomer concentration, ionic strength as well as surface charge, particle size and molecular weight are important for drug delivery. Further, these nanoparticles have the capability to reverse
multidrug resistance a major problem in chemotherapy. Well-established therapies commonly employed in cancer treatment include surgery, Chemotherapy, immunotherapy, and
radiotherapy. The silver nanoparticles might be involved in neutralizing these adhesive substances, thus preventing biofilm formation. Selenium is also one of essential trace elements in the human body and has great importance in nourishment and medicine. Medicaldiagnostic field also developed to use the selenium nanoparticles and also studies on the increase efficiency of glutathione peroxidase and thioredosin reductase.
Abstract book for the following conferences:
2013 2nd International Conference on Bioinformatics and Biomedical Science (ICBBS 2013)
2013 2nd International Conference on Environment, Energy and Biotechnology (ICEEB 2013)
2013 2nd International Conference on Chemical and Process Engineering (ICCPE 2013)
2013 2nd Journal Conference on Environmental Science and Development (JCESD 20132nd)
The conferences was held at Concorde Inn Kuala Lumpur International Airport, Malaysia on 09 June 2013
Identification of Rare and Novel Alleles in FFPE Tumor Samples | ESHG 2015 Po...Thermo Fisher Scientific
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QIVIVE extrapolation requires a precise correlation between exposure and the effective chemical concentration at the site where the MIE occurs.
This work demonstrates that intracellular distribution is not ruled only by physical-chemical parameters, rather it is mainly regulated by specific biological-mediated mechanisms. Substances with
apparent chemical similarity may show different distribution profile, as shown by the intra-nuclear distribution of polyphenols. While our results derive from a limited number of substances applied to
one cell line, it is plausible that using different substances and/or different cell lines would also have shown that intracellular distribution is not directly related to physical-chemical parameters.
Chemical uptake should be specifically measured and simple extrapolations based on physical-chemical properties may provide misleading decision
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in this research paper ,researchers found a new therapeutic drug that is SULFASALAZINE for the treatment of neuroblastoma that has a action on SEPIATERIN REDUCTASE
Our third webinar in the MDC Connects Series 2021 | A Guide to Complex Medicines.
This slide deck gives an overview of the early assessment of Prototype Nanomedicine Nano Bio Interactions.
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One of the most developed cities of India, the city of Chennai is the capital of Tamilnadu and many people from different parts of India come here to earn their bread and butter. Being a metropolitan, the city is filled with towering building and beaches but the sad part as with almost every Indian city
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2. Introduction
• After administration of nanoparticle into biological fluid, a
NP-Protein complex is formed (represents “true identity”);
called Protein corona
• This NP-Protein complex determines biological responses
like cellular uptake, circulation time, bioavailability &
toxicity
12. nanoparticles Physico chemical
properties that affect
protein adsorption
Major protein identity
in the corona
Biological
responses
Gold Size(15.13,60,90 nm),
Surface functional group
C3 complement protein Decreasing C3 binding with
increasing PEG grafting
density results in lowered
macrophage uptake of the
nnanoparticles
Poly (propylene sulfide) Surface functional group
(Pluronic F-127 & further
attachment of ovalbumin)
Recognition of the adjuvant
pluronic F-127 by C3b-
fragments
Uptake of the NPs by
dendritic cells via
recognition of the antigen
ovalbumin by Toll-like
receptors (TLRs); the TLRs
is activated upon pluronic-
F127-C3b recognition
Poly(butyl) cyanoacrylate Surface modification via
attachment of the surfactant
polysorbate 80
Preferential binding of
apolipoprotein E to surface
modified NPs
Receptor mediated endocytic
uptake of the NPs by brain
capillary endothelial cells
(BCECs)
Human Serum Albumin
(HAS); liposomes
- Artificial attachment of apo
E or apo-E derived synthetic
peptides
Receptor mediated endocytic
uptake of NPs by cerebral
epithelial cells
Chitosan (CS)-Dextran (DS)
sulfate
CS to DS weight ratio Artificial attachment of IgA Targeted delivery of the NPs
to M cells of NALT via IgA
recognition
13. Characterization of nanoparticle-protein corona
Corona parameter Techniques
Thickness Dynamic Light Scattering (DLS),
differential centrifugation sedimentation
(SEC), Transmission Electron
Microscopy (TEM)
Density Colorimetric protein assays
Identity & Quantity Polyacrylamide Gel Electrophoresis
(PAGE), Liquid chromatography tandem
mass spectroscopy (LC-MS/MS)
Confirmation Circular Dichroism (CD), Fluorescence
quenching, Computational stimulation
Affinity Size exclusion chromatography(SEC),
Surface Plasmon Resonance (SPR),
Isothermal titration calorimetry (ITC)
16. Effect of protein corona on nanoparticle cytotoxicity
Cell membrane rupture
(A) Cytotoxicity of GO nanosheets against A549 cells . AFM images of GO (upper left) and BSA
coated GO nanosheets (lower left). TEM images of A549 cells treated with 100ug/ml GO
nanosheets (upper right) and and BSA coated GO nanosheets (lower right) for 2 hr. Copyright,
Hu et al, 2011, ACS publications.
18. How does protein corona influence tumor
targeting???
• Typically, less than 10% of all systemically administered
nanoparticles accumulate in the tumour
• In the blood, serum proteins adsorb onto nanoparticles to
form a protein corona
• These serum proteins can block nanoparticle tumour
targeting ligands from binding to tumour cell receptors
• The formation of this protein corona will also increase the
nanoparticle hydrodynamic size or induce aggregation,
which makes nanoparticles too large to enter into the
tumour through pores of the leaky vessels, and prevents
their deep penetration into tumours
19. Nanovaccine Designing and Protein corona
• Hydrophobicity,
hydrophilicity
•Surface charge (Alb:
positive NP). Eg:
Deposition of albumin
onto nanogels was
prevented by loading the
nanogels with negatively
charged siRNA.
•PEGylation guaranteeing
a stealth-like behavior for
enhanced circulation
properties after systemic
administration
20. RESEARCH PAPER
Title:
Revealing the immune perturbation of
black phosphorus nanomaterials to
macrophages by understanding the
protein corona
21.
22. AIM:
To evaluate protein corona formed by interaction of
BP nanoparticles and plasma proteins and to
demonstrate their immune perturbation effect on
THP-1 cells.
HYPOTHESIS:
Evaluation of impact of nanoparticle –protein corona
on immune system.
23. Methodology
1. Synthesis and
Characterisation
2. Nanomaterial protein
interaction studies
3. Cellular internalisation of
BP nanomaterials and corona
complexes
4. Fluorescent
microscopic analysis
5. Immune
perturbation studies
6. Cytokine secretion
assays
24. RESULTS AND DISCUSSION
Summary of diagram of immune perturbation of BP-corona complexes.
Formation of BP nanomaterial-corona complexes in blood and their
immunoregulation on macrophages.
25. (a) TEM image of BPNS, scale bar 200nm. (b) TEM image of BPQD, scale bar 200nm. (c) Raman spectra of BPQD and
BPNS. (d) XPS spectra of BPQD and BPNS. (e) TEM image of BPNS-corona complex; scale bar 50nm. (f) TEM image
of BPQD-corona complex; scale bar 500nm. (g) DLS analysis of BPNS and BPNS-corona complexes. (h) DLS analysis
of BPQD and BPQD-corona complex. (i) Zeta potential of BP nanomaterials and BP-corona complexes.
26. SDS PAGE analysis of plasma proteins obtained from (a) BPNS-corona complex, (b) BPQD-corona complex. (c) and
(d) represents relative amounts of the most abundant proteins bound to BPNS and BPQD respectively.
BPNS-corona
BPQD-corona
27. (A) Statistical analysis of the total number of proteins identified by LC-MS/MS.
(B) Analysis of immune relevant protein fraction present in free plasma, BPQD-Corona
complexes and BPNS-Corona complexes
(A)
(B)
28. Cellular internalisation of BP nanomaterials and corona complexes. (a) cellular uptake of BP nanomaterials and
corona complexes. Macrophage like THP-1 cells were treated with 150μg/ml nanomaterials for 1,3 and 6hr. (b)
fluorescent microscopic staining cells treated with BPQD and BPNS corona complexes.
29. (a) Immune responses triggered by BPQD, BPQD-corona, BPNS and BPNS-corona complex with IL-1β, IL-6,
1L-8, IL-9, IL-10 and IFN- γ in Macrophage like THP-1 cells. Increasing concentration of 12.5, 25 and 50μg/ml
for 6hr.
30. (a) Immune responses triggered by BPQD, BPQD-corona complex, BPNS and BPNS-corona complex with
IL-1β, IL-6, 1L-8, IL-9, IL-10 and IFN- γ in SC cells (human macrophage from peripheral blood).
Increasing concentration of 12.5, 25 and 50μg/ml for 6hr.
31. ROS overproduction in macrophages exposed to 50μg/ml BP nanomaterials or BP corona
complexes for 6hr. LPS positive control.
32. Conclusions
• Study was carried out to provide a detailed account of BP-protein
corona and its impact on macrophages.
• Results showed that the size of 2D BP nanomaterials can influence
the identity and quantity of plasma proteins that form corona.
• Corona formation can define the shape of BPQDs but doesnot change
the shape of BPNS.
• Owing to the enrichment of immune proteins around nanoparticles, an
increased uptake efficiency of BP-corona complexes by macrophages
was found.
• Size dependant cellular uptake pattern of BP-corona complexes is
different from that of native BP nanoparticles.
• As the cellular uptake increased BP nanomaterials bound to opsonins
have a higher impact on proinflammatory and immune perturbation
effect on macrophages. This reveals the pivotal role of corona in
determining innate immune and inflammatory responses.
33. References
• Bisht, G. and Rayamajhi, S., 2016. ZnO nanoparticles: a promising
anticancer agent. Nanobiomedicine, 3(Godište 2016), pp.3-9.
• Cedervall, Tommy, Iseult Lynch, Stina Lindman, Tord Berggård, Eva
Thulin, Hanna Nilsson, Kenneth A. Dawson, and Sara Linse.
"Understanding the nanoparticle–protein corona using methods to
quantify exchange rates and affinities of proteins for
nanoparticles." Proceedings of the National Academy of Sciences 104,
no. 7 (2007): 2050-2055.
• Rahman MZ, Kwong CW, Davey K, Qiao SZ. 2D phosphorene as a
water splitting photocatalyst: fundamentals to applications. Energy &
Environmental Science. (2016);9(3):709-28.
• Van Hong Nguyen BJ. Protein corona: a new approach for nanomedicine
design. International journal of nanomedicine. 2017;12:3137.
• Gunawan C, Lim M, Marquis CP, Amal R. Nanoparticle–protein corona
complexes govern the biological fates and functions of nanoparticles.
Journal of Materials Chemistry B. 2014;2(15):2060-83.
Editor's Notes
Conventional vaccines that include a tumor antigen and an adjuvant do not specifically address specific types of APC . In addition, both components may dissociate and cause unwanted side effects .nanovaccines can facilitate co-delivery of antigen and adjuvant.
Thus, intracellular processing is as important as the vaccine dose that is taken up.
