This document discusses probiotics and their uses in food and agricultural industries. It defines probiotics as live microorganisms that provide health benefits when consumed. Common probiotic strains include Lactobacillus and Bifidobacterium. The document presents two case studies showing how probiotics can inhibit pathogenic bacteria. Probiotics protect intestinal health and enhance immune function. They are effective for controlling pathogens in poultry, livestock and aquaculture. Probiotics also inhibit biofilm formation by foodborne pathogens like E. coli and Salmonella, reducing food contamination.
Probiotics are useful microorganisms to fight against pathogenic bacteria in human gut . Commercially bacteria and fungus are being used as probiotic organisms
This is the unedited version of the "Probiotics" presentation given at the University of Arizona's College of Medicine. Its intent was to demonstrate that even in the "hard sciences" base emotional biases can interfere with good scientific data and interpretation. To fully appreciate this presentation, you must be eating yogurt prior to beginning to view it, and proceed through the slides one at a time without skipping ahead. This is the actual slide show that was shown at the seminar and is not for the squeamish. I'll post a more benign version for those not wishing to test their boundaries and "good taste."
Probiotics are useful microorganisms to fight against pathogenic bacteria in human gut . Commercially bacteria and fungus are being used as probiotic organisms
This is the unedited version of the "Probiotics" presentation given at the University of Arizona's College of Medicine. Its intent was to demonstrate that even in the "hard sciences" base emotional biases can interfere with good scientific data and interpretation. To fully appreciate this presentation, you must be eating yogurt prior to beginning to view it, and proceed through the slides one at a time without skipping ahead. This is the actual slide show that was shown at the seminar and is not for the squeamish. I'll post a more benign version for those not wishing to test their boundaries and "good taste."
covers Introduction.
History of probiotics.
General characteristics of probiotics.
Why are probiotics important to human health?
Some probiotics strains.
Mechanism of action
Advantages of host and type of advantages.
Classification of probiotics and examples.
Probiotics but not antibiotics, explanation with AAD.
Some probiotic food.
Probiotics and cancer.
Disadvantages of probiotics
Status of probiotics in india
India and global probiotics manufactures.
Beneficial Effects of Probiotics
Introduction: PROBIOTICS are living microorganisms, when taken by mouth, benefit your health by improving the balance of bacteria in the intestines.
Often bacteria, but also include some kinds of yeast.
PROBIOTICS are “good” bacteria or non-pathogenic in healthy people.
Gut contains over 500 different types of beneficial bacteria.
Beneficial functions such as inhibiting the growth of pathogenic bacteria, aiding in digestion, and vitamin-B synthesis.
To consider Bacterium as Probiotics, must meet the following criteria:
It is a microbial organism which is not harmful
It remains alive during processing and the shelf life of the food
It must survive digestion and remain alive in the gut
It is able to bring about a response in the gut
It is associated with health benefits
Common Probiotic Bacteria
Lactobacillus acidophilus
Lactobacillus casei
Lactobacillus reuteri
Lactobacillus plantarum
Lactobacillus rhamnosus
Bifidobacterium animalis
Bifidobacterium infantis
Bifidobacterium lactis
Bifidobacterium longum
Beneficial Effects of Probiotics
Absorbing and/or destroying toxins released by certain “bad” bacteria that can make you sick.
Producing substances that prevent infection.
Preventing harmful bacteria from attaching to the gut wall and growing there.
Boosting your immune system
Sending signals to your cells to strengthen the mucus in your intestine, which helps it act as a barrier against infection.
Production of B vitamins. Vitamin B is important in maintaining healthy skin, a healthy nervous system and preventing anemia
Decrease gas production and bloating
Safety and toxicity evaluation of probiotics foodsSukhveerSingh31
Probiotics can also be defined as “Live microorganisms that when administered in adequate amounts confer a health benefit on the host” (Council for Agricultural Science and Technology).
covers Introduction.
History of probiotics.
General characteristics of probiotics.
Why are probiotics important to human health?
Some probiotics strains.
Mechanism of action
Advantages of host and type of advantages.
Classification of probiotics and examples.
Probiotics but not antibiotics, explanation with AAD.
Some probiotic food.
Probiotics and cancer.
Disadvantages of probiotics
Status of probiotics in india
India and global probiotics manufactures.
Beneficial Effects of Probiotics
Introduction: PROBIOTICS are living microorganisms, when taken by mouth, benefit your health by improving the balance of bacteria in the intestines.
Often bacteria, but also include some kinds of yeast.
PROBIOTICS are “good” bacteria or non-pathogenic in healthy people.
Gut contains over 500 different types of beneficial bacteria.
Beneficial functions such as inhibiting the growth of pathogenic bacteria, aiding in digestion, and vitamin-B synthesis.
To consider Bacterium as Probiotics, must meet the following criteria:
It is a microbial organism which is not harmful
It remains alive during processing and the shelf life of the food
It must survive digestion and remain alive in the gut
It is able to bring about a response in the gut
It is associated with health benefits
Common Probiotic Bacteria
Lactobacillus acidophilus
Lactobacillus casei
Lactobacillus reuteri
Lactobacillus plantarum
Lactobacillus rhamnosus
Bifidobacterium animalis
Bifidobacterium infantis
Bifidobacterium lactis
Bifidobacterium longum
Beneficial Effects of Probiotics
Absorbing and/or destroying toxins released by certain “bad” bacteria that can make you sick.
Producing substances that prevent infection.
Preventing harmful bacteria from attaching to the gut wall and growing there.
Boosting your immune system
Sending signals to your cells to strengthen the mucus in your intestine, which helps it act as a barrier against infection.
Production of B vitamins. Vitamin B is important in maintaining healthy skin, a healthy nervous system and preventing anemia
Decrease gas production and bloating
Safety and toxicity evaluation of probiotics foodsSukhveerSingh31
Probiotics can also be defined as “Live microorganisms that when administered in adequate amounts confer a health benefit on the host” (Council for Agricultural Science and Technology).
How does probiotics help in paediatrics?
All you want to know about probiotics in paediatrics!
PPT made by @smsht ccl and presented by Dr. Rashmin Cecil
Neutraceuticals - Probiotics, Prebiotics & SynbioticsMayur D. Chauhan
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Mammalian Pineal Body Structure and Also Functions
Probiotic: An alternate bio-agent in food and agricultural industry
1.
2.
3. PROBIOTIC: AN ALTERNATE BIOAGENt IN FOODAND
AGRICULTURAL INDUSTRY
Krutika Patil
Division of Microbiology
ICAR-IARI
4. •The human microbiome
is the collection of all the
microbes found in or on
our bodies.
•Their number is ten
times the human cells.
•The gut microflora is a
main constituent of
intestine’s defence
system
HUMAN MICROBIOME
6. • The term Probiotic was coined in 1965 by Lilly and Stillwell.
• The term derived from Latin preposition ‘pro’ and Greek
adjective ‘bios’meaning “FOR LIFE”.
• Organism and substances that have a beneficial effect on the
host animal by contributing to its intestinal microbial balance-
Parker (1974)
• Live microbial feed supplement which beneficially affects the
host animal by improving its intestinal microbial balance-
Fuller (1989)
• Live organisms which, when administered in adequate
amount, confer a health benefit on the host –FAO/WHO
(2002)
PROBIOTICS
7. •Concept of probiotic
•Modify the gut flora and to replace harmful
microbes by useful microbes
•Intestinal autointoxication
•‘Bulgarian Bacillus’
Elie Metkinkoff (1907)
•First isolated Bifidobacterium
from breast fed infants
•Bacillus bifidus communis
•Clinical benefit-treating diarrhea
Henry Tissier (1906)
HISTORY
8. •Outbreak of Shigellosis
•Isolated strain of Escherichia
coli from faeces of a soldier
•E.coli Nessle 1917
•Yakult-a fermented milk product
•Lactobacillus casei shirota
Alfred Nissle (1917)
Dr.Minoru Shirota (1930)
9. What are the Properties of microorganisms to be called as Probiotic?
• Non-pathogenic and non-toxic
• Able to survive the passage through the digestive system
• Able to attach to the intestinal epithelia and colonise
• Able to maintain good viability
• Capable of exerting a beneficial effect on the host
• Stability of desired characteristics during processing, storage
and transportation
11. Bifidobacterium spp.,
• B. bifidum
• B. essencis
• B. infantis
• B. longum
• B. lactis
• B. breve
• B. animalis
12. Other microorganisms used as Probiotic:-
• Bacillus subtilis, Bacillus cereus
• Enterococcus faecalis
• Saccharomyces boulardii, S. cerevisiae
• Propionibacterium freudenreichii
• Leuconostoc mesenteroides
• Streptococcus salivarius
Bacillus spp.,
Enterococcus spp.,
Saccharomyces spp.,
13. importaNCE OF Probiotics
• Antibiotic and other drug intake
• Microbial infections
• Unhealthy diet
• Stress
• Age
• Colonic therapies for detoxification
14. MODE OF ACTION as bioagent
a) Competitive
exclusion of
pathogenic
Microorganisms
b) Production of
antimicrobial
substances.
c) Competition for
nutrients &
growth factors.
d) Increase adhesion
to intestinal mucosa.
e) Enhanced
epithelial barrier
function.
f) Enhanced IgA
secretion (Immune
stimulation)
20. CASE STUDY -1
OBJECTIVE
• To decipher the effect of oral intubation of Bacillus subtilis on
Aeromonas hydrophila-induced intestinal mucosal barrier function
damage and inflammation in grass carp
Weiguang Kong, Can Huang, Ying Tang, Ding Zhang, Zhixin Wu & Xiaoxuan Chen
(Kong et al., (2017) Scientific Reports 7: 1588 DOI:10.1038/s41598-017-01336-9)
21. MATERIALS & METHODS
1.Specimen: 2 year old Grass carp(Ctenopharyngodon idella)
2.Bacterial Strains: B. subtilis Ch9 and A. hydrophila
3.Infection experiment: 100L plastic tank with aerated tap water
a) control group
b) A. hydrophila group
c) B. subtilis + A. hydrophila
4.Sampling: 6 hpi, 12 hpi, 24 hpi, 48 hpi, 72 hpi, 96 hpi, and
120 hpi (hpi - hours post infection)
5. Detection of intestinal permeability:
a. serum D-lactic acid: fish D-lactic ELISA assay kit
b. Evans Blue(EB)
6. Histological assessment: 3D digital slice scanner
22. RESULT
Effect of A. hydrophila on the concentration of intestinal EB and
serum D-lactic acid after oral intubation with B. subtilis
23. Histological changes of the intestines 48 h after oral application
of A. hydrophila
Groups Intestinal villus Number per
villus
Number per
mm2
Length (μm) Width (μm) Goblet cells Inflammatory
cells
Control 367.62 ±
23.70
110.32 ±
7.80
34.3 ± 6.22 1357.05 ±
271.88
A. hydrophila 296.49 ±
19.62
171.75 ±
16.03
40.1 ± 7.43 4014.06 ±
872.12
B. subtilis + A.
hydrophila
375.71 ±
14.26
108.87 ±
7.02
63.6 ± 14.10 1922.84 ±
373.25
Effect of A. hydrophila on the intestinal length, width of villi, No.of villus of goblet,
inflammatory cells and after orally intubation with B. subtilis
A) Control
B) A.
hydrophila
C) B. subtilis +
A.
hydrophila
24. CONCLUSION
• The amount of EB and serum level of D-lactic acid
concentration was markedly higher in the A.hydrophila group
compared to control and protective group 48 hpi and 72 hpi
with A. hydrophila. This indicates that protective group having
B. subtilis could largely prevent the increase in intestinal
mucosal permeability caused by A.hydrophila.
• Disruption of intestinal barrier function is often accompanied
by intestinal inflammation. Administration of the probiotic B.
subtilis Ch9 prior to oral intubation could prevent functional
damage to intestinal mucosal barrier and reduced inflammation
induced by A. hydrophila in grass carp.
25. CASE STUDY-2
Natacha C. Gómez, Juan M. P. Ramiro, Beatriz X. V. Quecan and
Bernadette D. G. de Melo Franco
OBJECTIVE: To evaluate the potential probiotic traits of LAB isolated
from different fermented Brazilian products and their inhibition Effect
against Escherichia coli O157:H7, Listeria monocytogenes and S.
typhimurium biofilm formation.
(Gomez et al., (2016) Frontiers in Microbiology 7:863.doi: 10.3389/fmicb.2016.00863)
Received: 22 February 2016
Accepted: 23 May 2016
28. Co-Aggregation aggregation values recorded for lactic acid bacteria
strains with Listeria monocytogenes , Salmonella typhimurium and
Escherichia coli O157:H7 after 24h incubation at 37°C in PBS (pH 7.1)
30. A. Listeria
monocytogenes
1. Positive control
2. W. viridescens 113
3. Lactobacillus casei 40
4. Lactobacillus
helveticus 352
5. L. lactis 94
6. L. lactis 69
Quantification of pathogen biofilms in presence of probiotic strains
31. 1
1
1. Positive control B. S. Typhimurium
2. W. viridescens 113 C. E. coli O157:H7
3. Lactobacillus casei 40
4. Lactobacillus
helveticus 352
5. L. lactis 94
6. L. lactis 69
32. CONCLUSION
• Lactobacillus curvatus MBSa3 exhibited the highest co-aggregation (69%
with Listeria monocytogenes and 74.6% with E. coli O157:H7) and in this
case pathogenic biofilms were not detected after three times of incubation
tested, 24, 48,and 72 hours.
• The study showed the potential of probiotic LAB biofilms for the control of
Listeria monocytogenes, S. Typhimurium and E. coli O157:H7 biofilms
formation.
33. Conclusion
• Biocontrol property of some probiotics can be exploited
against certain pathogenic strains of bacteria.
• Due to the potential antimicrobial functions, probiotics can be
effectively used to control the pathogens of poultry, live stock
and aquaculture.
• Probiotics can be used as environment friendly agents for
controlling a number of microbial pathogens.
• They can be used to counteract the proliferation of several
bacteria which form biofilm leading to food contamination.
• Therefore, the probiotics offer great potential in food and
agriculture industry due to an array of applications they
possess.
34. Future DIRECTIONS
• More intensive studies are required on probiotics to enhance
their utility as biocontrol in food and agriculture industry
• The consumers must be made aware on the safety, efficacy,
sensory appeal, brand and marketing of probiotics for greater
acceptability.
• Detailed studies on probiotics in vivo is required to critically
evaluate the interaction between host and probiotic strains.
• Advanced throughput technologies to be conducted to
understand the molecular basis of relationship between
probiotic organisms and their host.