SlideShare a Scribd company logo
Identification of new microorganisms with
probiotic potential and activity against
enteropathogens
Dr. Herminio Mendes
Introduction
• What area probiotics?
• According to the Food and Agriculture Organization of the United Nations and the World Health
Organization (WHO), probiotics are live microorganisms that confer a health benefit to the host
when they are administered in adequate amounts (FAO/WHO, 2006).
• Specially, bacteria belonging to genus Bifidobacterium and Lactobacillus, which are the
predominant and subdominant groups on the gastrointestinal microbiota, respectively, are the
most widely used probiotic bacteria and are included in many functional foods and dietary
supplements (GOURBEYRE et al., 2011).
Lactobacillus Bifidobacterium
History of Probiotics
• Metchnikoff – acid producing bacteria in fermented milk products 1907
• Probiotics term coined in 1965 by Lilly And Stillwell
• Probiotics, which means "for life" was meant to contrast "antibiotics",
popularly prescribed and known to also destroy beneficial organisms
and impact the immune system.
Elie
Metchnikoff
Relevance
• Why are probiotics importante for human life?
Destroy the gut
microflora
Emergency of
antibiotic resistance
strains
Public health
problems
Antibiotics
Human diseases
Stress
delays in children's
linear and cognitive
development
Problems in
digestion
Problems in the
absortion of
nutrientes in the
body
Where we found probiotics?
• Generally, the selection of new probiotic strains for human use is sourced from human intestinal
flora and breast milk (MARTIN et al., 2009)
Intestinal microflora
With a long history of the safe use of
probiotics in fermented dairy products
and recognition of their beneficial effects
on human health, the food industry has
become increasingly interested in these
types of microorganisms
source of new species
Microflora Composition
• The initial composition of the gut microbiota begins to be established
concomitantly with birth and depends fundamentally on two factors:
DELIVERY MODE
Cesarian
Vaginal
NUTRITIONAL STATUS
OF THE MOTHER
• Normal Development of the Immune
System
• Production of specific cytokines for proper
immune system development
• Disrupted Intestinal Microbial Colonization
• Increase risk for Atopic Diseases, Asthma,
Allergic Rhinitis, and Celiac Disease
• Association: Delayed Onset of Lactation
• Lack Breast Milk Support for Gut Flora
Identification of new strains
• The criteria for the selection of probiotics include tolerance to gastrointestinal conditions (gastric
acid and bile), the ability to adhere to the gastrointestinal mucosa and competitive exclusion of
pathogens (SANDERS, 2013).
Diarrhoea is a leading killer of children,
accounting for approximately 9% of all deaths
among children under age 5 worldwide in
2022. This translates to over 1,300 young
children dying each day, or about 484,000
children a year, despite the availability of a
simple treatment solution
Doctorate Thesis
• Biological Samples – 30 samples
• The breastmilk from different stages (Colostrum, Transitional milk and Mature
Milk)
• Stools samples of exclusively breast-fed infants under 1 month of age.
Transitional milk
Colostrum Mature Milk
Total = 90 samples Total = 30 samples
Infant stools
Doctorate Thesis
• Bacterial Isolation, Probioct Evaluation and Molecular Identification
• Bacteria were isolated from stool samples and breastmilk samples, as previously described
(Thompson-Chagoyan et al., 2010) with modifications.
Evaluation of
probiotic potential
Antagonic
activity
Adhesion
assays
Resistant to bile
salt and gastric
juice
Antibiotic
susceptibility
test
Stools samples
And
milk samples
(PBS; pH 7.4)
MRS MRS
37 °C for 48-72 h
under anaerobic conditions
API 50 CHL Biochemical
characterization
Microscope
analysis
-80 °C
Bacterial cell
Futher analysis
MiSeq Platform (Illumina)
16s rRNA
Sequencing
Disc difusion
method CLSI 2012
HT-29 Cells and
Mucin Biding Assay
V3-V4
ETEC, EPEC,
EHEC, EAEC Enterpathopgens
What are the actions of probiotics?
Probiotics
mode of action
Modification of
microbial population
Modification of the structure
and function of intestinal
epithelium
Competition for
nutrients
Production of specific substances (organic acide,
bacteriocins, dipiocolinic acid
Aggregation with pathogenic
bacteria
Competitive adhesion to
epitelial receptors
Diarrheal disease
• In diarrhea, the effects of probiotics are not yet fully elucidated,
however, some works reports beneficial effects of probiotics on some
types of diarrhea.
Diarrheal disease
• Diarrheagenic Escherichia coli (DEC) are considered important etiologic agents of intestinal
infection worldwide, especially in children (Acosta et al. 2016, Al-Gallas et al. 2007, Bueris et
al.2007, Dias et al. 2016, Ieanyi et al. 2015, lijima et al. 2017).
• Several distinct clinical syndromes accompany infection with diarrheagenic E. coli categories,
including
traveler's diarrhea
(enterotoxigenic E.
coli)
hemorrhagic colitis and
hemolytic-uremic
syndrome
persistent diarrhea
(enteroaggregative E.
coli)
and watery diarrhea of
infants (entero-
pathogenic E. coli)
DEC
How probiotics work in the treatment and prevention
of diarrhea?
Competetive
exclusion of enteric
pathogens
Probiotics
Tiggers cytokine synthesis
from enterocytes by attaching
to their surface
Inhibit growth of potential
pathogens by producing
lactic acid, bacteriocin etc
Restore the normal
intestinal flora during
antibiotic therapy
Produce toxic metabolites
e.g hydrogen peroxide
Production of butyric acid
Increased turnover of
enterocytes
Neutralization of dietary
carcinogens
Thank you all
Lençóis Maranhenses National Park in Brazil

More Related Content

Similar to identification of new microorganisms with probiotic potential and.pptx

Probiotics ppt seminar prebiotics presentation
Probiotics ppt seminar prebiotics presentation Probiotics ppt seminar prebiotics presentation
Probiotics ppt seminar prebiotics presentation
naveen CHAUDHARY
 
Microbiota intestinale e Patologie Neurodegenerative
Microbiota intestinale e Patologie NeurodegenerativeMicrobiota intestinale e Patologie Neurodegenerative
Microbiota intestinale e Patologie Neurodegenerative
ASMaD
 
Probiotics
ProbioticsProbiotics
Probiotics
Atharva Chintawar
 
Probiotics ppt
Probiotics pptProbiotics ppt
Probiotics ppt
Deepak Sarangi
 
Probiotics by Akash Raza
Probiotics by Akash RazaProbiotics by Akash Raza
Probiotics by Akash Raza
akash raza
 
Probiotics1 12766811034852-phpapp01
Probiotics1 12766811034852-phpapp01Probiotics1 12766811034852-phpapp01
Probiotics1 12766811034852-phpapp01
akash raza
 
Prebiotics and Probiotics
Prebiotics and ProbioticsPrebiotics and Probiotics
Prebiotics and Probiotics
Rinisha Sinha
 
Dysbiosis & Probiotics Gyn Final (1) [Autosaved].pptx
Dysbiosis & Probiotics Gyn Final (1) [Autosaved].pptxDysbiosis & Probiotics Gyn Final (1) [Autosaved].pptx
Dysbiosis & Probiotics Gyn Final (1) [Autosaved].pptx
VidushRatan1
 
probioticsppt-160528055555.pptx
probioticsppt-160528055555.pptxprobioticsppt-160528055555.pptx
probioticsppt-160528055555.pptx
admissioncellHomesci
 
Probiotics
ProbioticsProbiotics
Probiotics
abeera fatima
 
Probiotics bhushan
Probiotics bhushanProbiotics bhushan
Probiotics bhushan
Bhushan Sanap
 
Probiotic
ProbioticProbiotic
Probiotic
Janak Shah
 
probiotics.pptx
probiotics.pptxprobiotics.pptx
probiotics.pptx
poonambansal32
 
Probiotics.farhat
Probiotics.farhatProbiotics.farhat
Probiotics.farhat
Dema Moheb
 
Prebiotics and probiotics
Prebiotics and probioticsPrebiotics and probiotics
Prebiotics and probiotics
Soumya Sahoo
 
Anticarcinogenic properties of probiotics
Anticarcinogenic properties  of probioticsAnticarcinogenic properties  of probiotics
Anticarcinogenic properties of probiotics
Debika Roy
 
Anticarcinogenic properties of probiotics
Anticarcinogenic properties  of probioticsAnticarcinogenic properties  of probiotics
Anticarcinogenic properties of probiotics
Debika Roy
 
Postbiotics in children
 Postbiotics in children Postbiotics in children
Postbiotics in children
Azad Haleem
 
Final diary products_as_carriers_of_probiotics_and_prebiotics[1]
Final diary products_as_carriers_of_probiotics_and_prebiotics[1]Final diary products_as_carriers_of_probiotics_and_prebiotics[1]
Final diary products_as_carriers_of_probiotics_and_prebiotics[1]
Michael Taiwo
 
Prebiotics and probiotics
Prebiotics and probioticsPrebiotics and probiotics
Prebiotics and probiotics
NivyaEM
 

Similar to identification of new microorganisms with probiotic potential and.pptx (20)

Probiotics ppt seminar prebiotics presentation
Probiotics ppt seminar prebiotics presentation Probiotics ppt seminar prebiotics presentation
Probiotics ppt seminar prebiotics presentation
 
Microbiota intestinale e Patologie Neurodegenerative
Microbiota intestinale e Patologie NeurodegenerativeMicrobiota intestinale e Patologie Neurodegenerative
Microbiota intestinale e Patologie Neurodegenerative
 
Probiotics
ProbioticsProbiotics
Probiotics
 
Probiotics ppt
Probiotics pptProbiotics ppt
Probiotics ppt
 
Probiotics by Akash Raza
Probiotics by Akash RazaProbiotics by Akash Raza
Probiotics by Akash Raza
 
Probiotics1 12766811034852-phpapp01
Probiotics1 12766811034852-phpapp01Probiotics1 12766811034852-phpapp01
Probiotics1 12766811034852-phpapp01
 
Prebiotics and Probiotics
Prebiotics and ProbioticsPrebiotics and Probiotics
Prebiotics and Probiotics
 
Dysbiosis & Probiotics Gyn Final (1) [Autosaved].pptx
Dysbiosis & Probiotics Gyn Final (1) [Autosaved].pptxDysbiosis & Probiotics Gyn Final (1) [Autosaved].pptx
Dysbiosis & Probiotics Gyn Final (1) [Autosaved].pptx
 
probioticsppt-160528055555.pptx
probioticsppt-160528055555.pptxprobioticsppt-160528055555.pptx
probioticsppt-160528055555.pptx
 
Probiotics
ProbioticsProbiotics
Probiotics
 
Probiotics bhushan
Probiotics bhushanProbiotics bhushan
Probiotics bhushan
 
Probiotic
ProbioticProbiotic
Probiotic
 
probiotics.pptx
probiotics.pptxprobiotics.pptx
probiotics.pptx
 
Probiotics.farhat
Probiotics.farhatProbiotics.farhat
Probiotics.farhat
 
Prebiotics and probiotics
Prebiotics and probioticsPrebiotics and probiotics
Prebiotics and probiotics
 
Anticarcinogenic properties of probiotics
Anticarcinogenic properties  of probioticsAnticarcinogenic properties  of probiotics
Anticarcinogenic properties of probiotics
 
Anticarcinogenic properties of probiotics
Anticarcinogenic properties  of probioticsAnticarcinogenic properties  of probiotics
Anticarcinogenic properties of probiotics
 
Postbiotics in children
 Postbiotics in children Postbiotics in children
Postbiotics in children
 
Final diary products_as_carriers_of_probiotics_and_prebiotics[1]
Final diary products_as_carriers_of_probiotics_and_prebiotics[1]Final diary products_as_carriers_of_probiotics_and_prebiotics[1]
Final diary products_as_carriers_of_probiotics_and_prebiotics[1]
 
Prebiotics and probiotics
Prebiotics and probioticsPrebiotics and probiotics
Prebiotics and probiotics
 

More from HerminioMendes3

AULA SOBRE herancamonogenica-160830171513.ppt
AULA SOBRE herancamonogenica-160830171513.pptAULA SOBRE herancamonogenica-160830171513.ppt
AULA SOBRE herancamonogenica-160830171513.ppt
HerminioMendes3
 
Os Padrões e os Processos da Evolução.pptx
Os Padrões e os Processos da Evolução.pptxOs Padrões e os Processos da Evolução.pptx
Os Padrões e os Processos da Evolução.pptx
HerminioMendes3
 
FORMAÇAO biossegurança e boas praticas MOMENTO 2.pptx
FORMAÇAO biossegurança e boas praticas MOMENTO 2.pptxFORMAÇAO biossegurança e boas praticas MOMENTO 2.pptx
FORMAÇAO biossegurança e boas praticas MOMENTO 2.pptx
HerminioMendes3
 
genetica conceitos basicos (2020_03_19 15_11_22 UTC).ppt
genetica conceitos  basicos (2020_03_19 15_11_22 UTC).pptgenetica conceitos  basicos (2020_03_19 15_11_22 UTC).ppt
genetica conceitos basicos (2020_03_19 15_11_22 UTC).ppt
HerminioMendes3
 
Aula_5 Genetica e Embriologia (2020_03_19 15_11_22 UTC).ppt
Aula_5 Genetica e Embriologia (2020_03_19 15_11_22 UTC).pptAula_5 Genetica e Embriologia (2020_03_19 15_11_22 UTC).ppt
Aula_5 Genetica e Embriologia (2020_03_19 15_11_22 UTC).ppt
HerminioMendes3
 
aula sobre SEDE E fisiologia da INGESTAO.pdf
aula sobre SEDE E fisiologia da INGESTAO.pdfaula sobre SEDE E fisiologia da INGESTAO.pdf
aula sobre SEDE E fisiologia da INGESTAO.pdf
HerminioMendes3
 
Saúde Coletiva - Aula NASSAU - ESTRATEGIAS
Saúde Coletiva - Aula NASSAU - ESTRATEGIASSaúde Coletiva - Aula NASSAU - ESTRATEGIAS
Saúde Coletiva - Aula NASSAU - ESTRATEGIAS
HerminioMendes3
 
celulas do sistema imune e suas principais características de
celulas do sistema imune e suas principais características decelulas do sistema imune e suas principais características de
celulas do sistema imune e suas principais características de
HerminioMendes3
 
Introdução Anatomia Humana e suas características
Introdução Anatomia Humana e suas característicasIntrodução Anatomia Humana e suas características
Introdução Anatomia Humana e suas características
HerminioMendes3
 
Formação em Biossegurança e Boas Práticas Laboratoriais 2.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais 2.pptxFormação em Biossegurança e Boas Práticas Laboratoriais 2.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais 2.pptx
HerminioMendes3
 
Formação em Biossegurança e Boas Práticas Laboratoriais.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais.pptxFormação em Biossegurança e Boas Práticas Laboratoriais.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais.pptx
HerminioMendes3
 
Formação em Biossegurança e Boas Práticas Laboratoriais - Copia.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais - Copia.pptxFormação em Biossegurança e Boas Práticas Laboratoriais - Copia.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais - Copia.pptx
HerminioMendes3
 
mapa e mapeamento de riscos ambientais aula
mapa e mapeamento de riscos ambientais aulamapa e mapeamento de riscos ambientais aula
mapa e mapeamento de riscos ambientais aula
HerminioMendes3
 
CONTEÚDO-PROGRAMÁTICO-IEMA-atualizado (2).pdf
CONTEÚDO-PROGRAMÁTICO-IEMA-atualizado (2).pdfCONTEÚDO-PROGRAMÁTICO-IEMA-atualizado (2).pdf
CONTEÚDO-PROGRAMÁTICO-IEMA-atualizado (2).pdf
HerminioMendes3
 
aula sobre gametogenese e fertilização sistema saude
aula sobre gametogenese e fertilização sistema saudeaula sobre gametogenese e fertilização sistema saude
aula sobre gametogenese e fertilização sistema saude
HerminioMendes3
 

More from HerminioMendes3 (15)

AULA SOBRE herancamonogenica-160830171513.ppt
AULA SOBRE herancamonogenica-160830171513.pptAULA SOBRE herancamonogenica-160830171513.ppt
AULA SOBRE herancamonogenica-160830171513.ppt
 
Os Padrões e os Processos da Evolução.pptx
Os Padrões e os Processos da Evolução.pptxOs Padrões e os Processos da Evolução.pptx
Os Padrões e os Processos da Evolução.pptx
 
FORMAÇAO biossegurança e boas praticas MOMENTO 2.pptx
FORMAÇAO biossegurança e boas praticas MOMENTO 2.pptxFORMAÇAO biossegurança e boas praticas MOMENTO 2.pptx
FORMAÇAO biossegurança e boas praticas MOMENTO 2.pptx
 
genetica conceitos basicos (2020_03_19 15_11_22 UTC).ppt
genetica conceitos  basicos (2020_03_19 15_11_22 UTC).pptgenetica conceitos  basicos (2020_03_19 15_11_22 UTC).ppt
genetica conceitos basicos (2020_03_19 15_11_22 UTC).ppt
 
Aula_5 Genetica e Embriologia (2020_03_19 15_11_22 UTC).ppt
Aula_5 Genetica e Embriologia (2020_03_19 15_11_22 UTC).pptAula_5 Genetica e Embriologia (2020_03_19 15_11_22 UTC).ppt
Aula_5 Genetica e Embriologia (2020_03_19 15_11_22 UTC).ppt
 
aula sobre SEDE E fisiologia da INGESTAO.pdf
aula sobre SEDE E fisiologia da INGESTAO.pdfaula sobre SEDE E fisiologia da INGESTAO.pdf
aula sobre SEDE E fisiologia da INGESTAO.pdf
 
Saúde Coletiva - Aula NASSAU - ESTRATEGIAS
Saúde Coletiva - Aula NASSAU - ESTRATEGIASSaúde Coletiva - Aula NASSAU - ESTRATEGIAS
Saúde Coletiva - Aula NASSAU - ESTRATEGIAS
 
celulas do sistema imune e suas principais características de
celulas do sistema imune e suas principais características decelulas do sistema imune e suas principais características de
celulas do sistema imune e suas principais características de
 
Introdução Anatomia Humana e suas características
Introdução Anatomia Humana e suas característicasIntrodução Anatomia Humana e suas características
Introdução Anatomia Humana e suas características
 
Formação em Biossegurança e Boas Práticas Laboratoriais 2.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais 2.pptxFormação em Biossegurança e Boas Práticas Laboratoriais 2.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais 2.pptx
 
Formação em Biossegurança e Boas Práticas Laboratoriais.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais.pptxFormação em Biossegurança e Boas Práticas Laboratoriais.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais.pptx
 
Formação em Biossegurança e Boas Práticas Laboratoriais - Copia.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais - Copia.pptxFormação em Biossegurança e Boas Práticas Laboratoriais - Copia.pptx
Formação em Biossegurança e Boas Práticas Laboratoriais - Copia.pptx
 
mapa e mapeamento de riscos ambientais aula
mapa e mapeamento de riscos ambientais aulamapa e mapeamento de riscos ambientais aula
mapa e mapeamento de riscos ambientais aula
 
CONTEÚDO-PROGRAMÁTICO-IEMA-atualizado (2).pdf
CONTEÚDO-PROGRAMÁTICO-IEMA-atualizado (2).pdfCONTEÚDO-PROGRAMÁTICO-IEMA-atualizado (2).pdf
CONTEÚDO-PROGRAMÁTICO-IEMA-atualizado (2).pdf
 
aula sobre gametogenese e fertilização sistema saude
aula sobre gametogenese e fertilização sistema saudeaula sobre gametogenese e fertilização sistema saude
aula sobre gametogenese e fertilização sistema saude
 

Recently uploaded

ColombiaPresentation.pptx macroeconomics
ColombiaPresentation.pptx macroeconomicsColombiaPresentation.pptx macroeconomics
ColombiaPresentation.pptx macroeconomics
JuanFelipeHerrera4
 
一比一原版(uoit毕业证书)加拿大安大略理工大学毕业证如何办理
一比一原版(uoit毕业证书)加拿大安大略理工大学毕业证如何办理一比一原版(uoit毕业证书)加拿大安大略理工大学毕业证如何办理
一比一原版(uoit毕业证书)加拿大安大略理工大学毕业证如何办理
vfefek
 
The cost of poor health: What does rising health-related benefit spending mea...
The cost of poor health: What does rising health-related benefit spending mea...The cost of poor health: What does rising health-related benefit spending mea...
The cost of poor health: What does rising health-related benefit spending mea...
ResolutionFoundation
 
Full Night Fun With Call Girls Hyderabad📞7737669865 At Very Cheap Rates Doors...
Full Night Fun With Call Girls Hyderabad📞7737669865 At Very Cheap Rates Doors...Full Night Fun With Call Girls Hyderabad📞7737669865 At Very Cheap Rates Doors...
Full Night Fun With Call Girls Hyderabad📞7737669865 At Very Cheap Rates Doors...
gourkajal4343
 
Jabatan Fungsional: Konsep, Peran & Prospeknya
Jabatan Fungsional: Konsep, Peran & ProspeknyaJabatan Fungsional: Konsep, Peran & Prospeknya
Jabatan Fungsional: Konsep, Peran & Prospeknya
Tri Widodo W. UTOMO
 
7339748667 Call Girls Kolkata with Trusted Models
 7339748667 Call Girls Kolkata with Trusted Models 7339748667 Call Girls Kolkata with Trusted Models
7339748667 Call Girls Kolkata with Trusted Models
Russian Escorts Delhi | 9711199171 | To Enjoy Every Moments Of Life!
 
Spending in the 340B Drug Pricing Program, 2010 to 2021
Spending in the 340B Drug Pricing Program, 2010 to 2021Spending in the 340B Drug Pricing Program, 2010 to 2021
Spending in the 340B Drug Pricing Program, 2010 to 2021
Congressional Budget Office
 
Health Insurance Coverage for the U.S. Population, 2024 to 2034
Health Insurance Coverage for the U.S. Population, 2024 to 2034Health Insurance Coverage for the U.S. Population, 2024 to 2034
Health Insurance Coverage for the U.S. Population, 2024 to 2034
Congressional Budget Office
 
UN SDSN Sustainable Development Report 2024
UN SDSN Sustainable Development Report 2024UN SDSN Sustainable Development Report 2024
UN SDSN Sustainable Development Report 2024
Energy for One World
 
Luxury Call Girls Noida {9873940964} VVIP POOJA Call Girls in Noida
Luxury Call Girls Noida {9873940964} VVIP POOJA Call Girls in NoidaLuxury Call Girls Noida {9873940964} VVIP POOJA Call Girls in Noida
Luxury Call Girls Noida {9873940964} VVIP POOJA Call Girls in Noida
gurkirankumar98700
 
CBO's Immigration Projections - Presentation
CBO's Immigration Projections - PresentationCBO's Immigration Projections - Presentation
CBO's Immigration Projections - Presentation
Congressional Budget Office
 
GRASSROOT-MODEL GRASSROOT-MODEL GRASSROOT-MODEL.pptx
GRASSROOT-MODEL GRASSROOT-MODEL GRASSROOT-MODEL.pptxGRASSROOT-MODEL GRASSROOT-MODEL GRASSROOT-MODEL.pptx
GRASSROOT-MODEL GRASSROOT-MODEL GRASSROOT-MODEL.pptx
Gavin Malala
 
EI Statistical Review of World Energy 2024
EI Statistical Review of World Energy 2024EI Statistical Review of World Energy 2024
EI Statistical Review of World Energy 2024
Energy for One World
 
一比一原版(theauckland毕业证书)新西兰奥克兰大学毕业证成绩单如何办理
一比一原版(theauckland毕业证书)新西兰奥克兰大学毕业证成绩单如何办理一比一原版(theauckland毕业证书)新西兰奥克兰大学毕业证成绩单如何办理
一比一原版(theauckland毕业证书)新西兰奥克兰大学毕业证成绩单如何办理
odmqk
 
一比一原版办理(UQ毕业证)昆士兰大学毕业证
一比一原版办理(UQ毕业证)昆士兰大学毕业证一比一原版办理(UQ毕业证)昆士兰大学毕业证
一比一原版办理(UQ毕业证)昆士兰大学毕业证
eesme1
 
SPONSORED CONTENT - Palmetier Law - Unleashing Small Business Innovations: A ...
SPONSORED CONTENT - Palmetier Law - Unleashing Small Business Innovations: A ...SPONSORED CONTENT - Palmetier Law - Unleashing Small Business Innovations: A ...
SPONSORED CONTENT - Palmetier Law - Unleashing Small Business Innovations: A ...
JSchaus & Associates
 
Call Girls in Kolkata (West Bengal) call me [🔝9352988975🔝] Escort In Kolkata ...
Call Girls in Kolkata (West Bengal) call me [🔝9352988975🔝] Escort In Kolkata ...Call Girls in Kolkata (West Bengal) call me [🔝9352988975🔝] Escort In Kolkata ...
Call Girls in Kolkata (West Bengal) call me [🔝9352988975🔝] Escort In Kolkata ...
deepakrana121234
 
Russian Call Girls Visakhapatnam 8800000000 Low Rate HIgh Profile Visakhapatn...
Russian Call Girls Visakhapatnam 8800000000 Low Rate HIgh Profile Visakhapatn...Russian Call Girls Visakhapatnam 8800000000 Low Rate HIgh Profile Visakhapatn...
Russian Call Girls Visakhapatnam 8800000000 Low Rate HIgh Profile Visakhapatn...
khannsohil539
 
Call Girls Kolkata { 7014168258 } Book High Class Models In Kolkata
Call Girls Kolkata { 7014168258 } Book High Class Models In KolkataCall Girls Kolkata { 7014168258 } Book High Class Models In Kolkata
Call Girls Kolkata { 7014168258 } Book High Class Models In Kolkata
aakashkumar5545
 
Causes Supporting Charity for Elderly People
Causes Supporting Charity for Elderly PeopleCauses Supporting Charity for Elderly People
Causes Supporting Charity for Elderly People
SERUDS INDIA
 

Recently uploaded (20)

ColombiaPresentation.pptx macroeconomics
ColombiaPresentation.pptx macroeconomicsColombiaPresentation.pptx macroeconomics
ColombiaPresentation.pptx macroeconomics
 
一比一原版(uoit毕业证书)加拿大安大略理工大学毕业证如何办理
一比一原版(uoit毕业证书)加拿大安大略理工大学毕业证如何办理一比一原版(uoit毕业证书)加拿大安大略理工大学毕业证如何办理
一比一原版(uoit毕业证书)加拿大安大略理工大学毕业证如何办理
 
The cost of poor health: What does rising health-related benefit spending mea...
The cost of poor health: What does rising health-related benefit spending mea...The cost of poor health: What does rising health-related benefit spending mea...
The cost of poor health: What does rising health-related benefit spending mea...
 
Full Night Fun With Call Girls Hyderabad📞7737669865 At Very Cheap Rates Doors...
Full Night Fun With Call Girls Hyderabad📞7737669865 At Very Cheap Rates Doors...Full Night Fun With Call Girls Hyderabad📞7737669865 At Very Cheap Rates Doors...
Full Night Fun With Call Girls Hyderabad📞7737669865 At Very Cheap Rates Doors...
 
Jabatan Fungsional: Konsep, Peran & Prospeknya
Jabatan Fungsional: Konsep, Peran & ProspeknyaJabatan Fungsional: Konsep, Peran & Prospeknya
Jabatan Fungsional: Konsep, Peran & Prospeknya
 
7339748667 Call Girls Kolkata with Trusted Models
 7339748667 Call Girls Kolkata with Trusted Models 7339748667 Call Girls Kolkata with Trusted Models
7339748667 Call Girls Kolkata with Trusted Models
 
Spending in the 340B Drug Pricing Program, 2010 to 2021
Spending in the 340B Drug Pricing Program, 2010 to 2021Spending in the 340B Drug Pricing Program, 2010 to 2021
Spending in the 340B Drug Pricing Program, 2010 to 2021
 
Health Insurance Coverage for the U.S. Population, 2024 to 2034
Health Insurance Coverage for the U.S. Population, 2024 to 2034Health Insurance Coverage for the U.S. Population, 2024 to 2034
Health Insurance Coverage for the U.S. Population, 2024 to 2034
 
UN SDSN Sustainable Development Report 2024
UN SDSN Sustainable Development Report 2024UN SDSN Sustainable Development Report 2024
UN SDSN Sustainable Development Report 2024
 
Luxury Call Girls Noida {9873940964} VVIP POOJA Call Girls in Noida
Luxury Call Girls Noida {9873940964} VVIP POOJA Call Girls in NoidaLuxury Call Girls Noida {9873940964} VVIP POOJA Call Girls in Noida
Luxury Call Girls Noida {9873940964} VVIP POOJA Call Girls in Noida
 
CBO's Immigration Projections - Presentation
CBO's Immigration Projections - PresentationCBO's Immigration Projections - Presentation
CBO's Immigration Projections - Presentation
 
GRASSROOT-MODEL GRASSROOT-MODEL GRASSROOT-MODEL.pptx
GRASSROOT-MODEL GRASSROOT-MODEL GRASSROOT-MODEL.pptxGRASSROOT-MODEL GRASSROOT-MODEL GRASSROOT-MODEL.pptx
GRASSROOT-MODEL GRASSROOT-MODEL GRASSROOT-MODEL.pptx
 
EI Statistical Review of World Energy 2024
EI Statistical Review of World Energy 2024EI Statistical Review of World Energy 2024
EI Statistical Review of World Energy 2024
 
一比一原版(theauckland毕业证书)新西兰奥克兰大学毕业证成绩单如何办理
一比一原版(theauckland毕业证书)新西兰奥克兰大学毕业证成绩单如何办理一比一原版(theauckland毕业证书)新西兰奥克兰大学毕业证成绩单如何办理
一比一原版(theauckland毕业证书)新西兰奥克兰大学毕业证成绩单如何办理
 
一比一原版办理(UQ毕业证)昆士兰大学毕业证
一比一原版办理(UQ毕业证)昆士兰大学毕业证一比一原版办理(UQ毕业证)昆士兰大学毕业证
一比一原版办理(UQ毕业证)昆士兰大学毕业证
 
SPONSORED CONTENT - Palmetier Law - Unleashing Small Business Innovations: A ...
SPONSORED CONTENT - Palmetier Law - Unleashing Small Business Innovations: A ...SPONSORED CONTENT - Palmetier Law - Unleashing Small Business Innovations: A ...
SPONSORED CONTENT - Palmetier Law - Unleashing Small Business Innovations: A ...
 
Call Girls in Kolkata (West Bengal) call me [🔝9352988975🔝] Escort In Kolkata ...
Call Girls in Kolkata (West Bengal) call me [🔝9352988975🔝] Escort In Kolkata ...Call Girls in Kolkata (West Bengal) call me [🔝9352988975🔝] Escort In Kolkata ...
Call Girls in Kolkata (West Bengal) call me [🔝9352988975🔝] Escort In Kolkata ...
 
Russian Call Girls Visakhapatnam 8800000000 Low Rate HIgh Profile Visakhapatn...
Russian Call Girls Visakhapatnam 8800000000 Low Rate HIgh Profile Visakhapatn...Russian Call Girls Visakhapatnam 8800000000 Low Rate HIgh Profile Visakhapatn...
Russian Call Girls Visakhapatnam 8800000000 Low Rate HIgh Profile Visakhapatn...
 
Call Girls Kolkata { 7014168258 } Book High Class Models In Kolkata
Call Girls Kolkata { 7014168258 } Book High Class Models In KolkataCall Girls Kolkata { 7014168258 } Book High Class Models In Kolkata
Call Girls Kolkata { 7014168258 } Book High Class Models In Kolkata
 
Causes Supporting Charity for Elderly People
Causes Supporting Charity for Elderly PeopleCauses Supporting Charity for Elderly People
Causes Supporting Charity for Elderly People
 

identification of new microorganisms with probiotic potential and.pptx

  • 1. Identification of new microorganisms with probiotic potential and activity against enteropathogens Dr. Herminio Mendes
  • 2. Introduction • What area probiotics? • According to the Food and Agriculture Organization of the United Nations and the World Health Organization (WHO), probiotics are live microorganisms that confer a health benefit to the host when they are administered in adequate amounts (FAO/WHO, 2006). • Specially, bacteria belonging to genus Bifidobacterium and Lactobacillus, which are the predominant and subdominant groups on the gastrointestinal microbiota, respectively, are the most widely used probiotic bacteria and are included in many functional foods and dietary supplements (GOURBEYRE et al., 2011). Lactobacillus Bifidobacterium
  • 3. History of Probiotics • Metchnikoff – acid producing bacteria in fermented milk products 1907 • Probiotics term coined in 1965 by Lilly And Stillwell • Probiotics, which means "for life" was meant to contrast "antibiotics", popularly prescribed and known to also destroy beneficial organisms and impact the immune system. Elie Metchnikoff
  • 4. Relevance • Why are probiotics importante for human life? Destroy the gut microflora Emergency of antibiotic resistance strains Public health problems Antibiotics Human diseases Stress delays in children's linear and cognitive development Problems in digestion Problems in the absortion of nutrientes in the body
  • 5. Where we found probiotics? • Generally, the selection of new probiotic strains for human use is sourced from human intestinal flora and breast milk (MARTIN et al., 2009) Intestinal microflora With a long history of the safe use of probiotics in fermented dairy products and recognition of their beneficial effects on human health, the food industry has become increasingly interested in these types of microorganisms source of new species
  • 6. Microflora Composition • The initial composition of the gut microbiota begins to be established concomitantly with birth and depends fundamentally on two factors: DELIVERY MODE Cesarian Vaginal NUTRITIONAL STATUS OF THE MOTHER • Normal Development of the Immune System • Production of specific cytokines for proper immune system development • Disrupted Intestinal Microbial Colonization • Increase risk for Atopic Diseases, Asthma, Allergic Rhinitis, and Celiac Disease • Association: Delayed Onset of Lactation • Lack Breast Milk Support for Gut Flora
  • 7. Identification of new strains • The criteria for the selection of probiotics include tolerance to gastrointestinal conditions (gastric acid and bile), the ability to adhere to the gastrointestinal mucosa and competitive exclusion of pathogens (SANDERS, 2013). Diarrhoea is a leading killer of children, accounting for approximately 9% of all deaths among children under age 5 worldwide in 2022. This translates to over 1,300 young children dying each day, or about 484,000 children a year, despite the availability of a simple treatment solution
  • 8. Doctorate Thesis • Biological Samples – 30 samples • The breastmilk from different stages (Colostrum, Transitional milk and Mature Milk) • Stools samples of exclusively breast-fed infants under 1 month of age. Transitional milk Colostrum Mature Milk Total = 90 samples Total = 30 samples Infant stools
  • 9. Doctorate Thesis • Bacterial Isolation, Probioct Evaluation and Molecular Identification • Bacteria were isolated from stool samples and breastmilk samples, as previously described (Thompson-Chagoyan et al., 2010) with modifications. Evaluation of probiotic potential Antagonic activity Adhesion assays Resistant to bile salt and gastric juice Antibiotic susceptibility test Stools samples And milk samples (PBS; pH 7.4) MRS MRS 37 °C for 48-72 h under anaerobic conditions API 50 CHL Biochemical characterization Microscope analysis -80 °C Bacterial cell Futher analysis MiSeq Platform (Illumina) 16s rRNA Sequencing Disc difusion method CLSI 2012 HT-29 Cells and Mucin Biding Assay V3-V4 ETEC, EPEC, EHEC, EAEC Enterpathopgens
  • 10. What are the actions of probiotics? Probiotics mode of action Modification of microbial population Modification of the structure and function of intestinal epithelium Competition for nutrients Production of specific substances (organic acide, bacteriocins, dipiocolinic acid Aggregation with pathogenic bacteria Competitive adhesion to epitelial receptors
  • 11. Diarrheal disease • In diarrhea, the effects of probiotics are not yet fully elucidated, however, some works reports beneficial effects of probiotics on some types of diarrhea.
  • 12. Diarrheal disease • Diarrheagenic Escherichia coli (DEC) are considered important etiologic agents of intestinal infection worldwide, especially in children (Acosta et al. 2016, Al-Gallas et al. 2007, Bueris et al.2007, Dias et al. 2016, Ieanyi et al. 2015, lijima et al. 2017). • Several distinct clinical syndromes accompany infection with diarrheagenic E. coli categories, including traveler's diarrhea (enterotoxigenic E. coli) hemorrhagic colitis and hemolytic-uremic syndrome persistent diarrhea (enteroaggregative E. coli) and watery diarrhea of infants (entero- pathogenic E. coli) DEC
  • 13. How probiotics work in the treatment and prevention of diarrhea? Competetive exclusion of enteric pathogens Probiotics Tiggers cytokine synthesis from enterocytes by attaching to their surface Inhibit growth of potential pathogens by producing lactic acid, bacteriocin etc Restore the normal intestinal flora during antibiotic therapy Produce toxic metabolites e.g hydrogen peroxide Production of butyric acid Increased turnover of enterocytes Neutralization of dietary carcinogens
  • 14. Thank you all Lençóis Maranhenses National Park in Brazil