A presentation on Photoacoustic Spectroscopy by Deepak Rajput, UT Space Institute, TN, USA.
This presentation was made as a course requirement at the University of Tennessee Space Institute at Tullahoma.
A presentation on Photoacoustic Spectroscopy by Deepak Rajput, UT Space Institute, TN, USA.
This presentation was made as a course requirement at the University of Tennessee Space Institute at Tullahoma.
Fluorescence spectroscopy becomes a widely used tool at the interface of biology, chemistry and physics, because of its precise sensitivity and recent technical advancements. The measurements can provide information on a wide range of molecular processes including the interactions of solvent molecules with fluorophores, rotational diffraction of biomolecules, distance between sites of biomolecules, conformational changes and binding interactions. These advances in fluorescence technology are decreasing the cost and complexity of previously complex processes. Fluorescence spectroscopy is a highly developed and non-invasive technique that enables the on-line measurements of substrate and product concentrations or the identification of characteristic process states.
Atomic absorption spectroscopy, History, atomization techniques, and instrume...Muhammad Asif Shaheeen
History, principle, types, instrumentation, comparison with atomic emission spectroscopy, interference, advantages and disadvantages of different types of atomization techniques.
Spectroscopy using spectrophotometers of different types like: U.V, Mass Spectrophotometer, absorption , Emission, Nuclear magnetic resonance and X-rays Spectrophotometer
Fluorescence spectroscopy becomes a widely used tool at the interface of biology, chemistry and physics, because of its precise sensitivity and recent technical advancements. The measurements can provide information on a wide range of molecular processes including the interactions of solvent molecules with fluorophores, rotational diffraction of biomolecules, distance between sites of biomolecules, conformational changes and binding interactions. These advances in fluorescence technology are decreasing the cost and complexity of previously complex processes. Fluorescence spectroscopy is a highly developed and non-invasive technique that enables the on-line measurements of substrate and product concentrations or the identification of characteristic process states.
Atomic absorption spectroscopy, History, atomization techniques, and instrume...Muhammad Asif Shaheeen
History, principle, types, instrumentation, comparison with atomic emission spectroscopy, interference, advantages and disadvantages of different types of atomization techniques.
Spectroscopy using spectrophotometers of different types like: U.V, Mass Spectrophotometer, absorption , Emission, Nuclear magnetic resonance and X-rays Spectrophotometer
UV-Visible spectroscopy is considered as an important tool in the analytical chemistry.
Most powerful tool available for the study of atomic and molecular structure.
- Most commonly used techniques in clinical as well as chemical laboratories.
- Used for the qualitative analysis and identification of chemicals.
ain use is for quantitative determination of different organic and inorganic compounds in solution.
Basically, spectroscopy is related to the interaction of light with matter.
As light is absorbed by matter, the result is an increase in the energy content of the atoms or molecules.
The absorption of visible or ultraviolet light by a chemical compound will produce a distinct spectrum.
UV-Visible light range- 200-800 nm
Visible range: 400-800 nm
UV range: 200-400 nm
The detailed information of UV Visible Spectroscopy, it includes the information regarding electronic transitions, Electromagnetic radiations, Various shifts.
UV - Visible Spectroscopy detailed information is included .The Spectroscopy study provide the information and the absorbance as well the concentration of the drugs is studied.
principle, application and instrumentation of UV- visible Spectrophotometer Ayetenew Abita Desa
This Presentation powerpoint includes the principle, application, and instrumentation of UV- Visible Spectrophotometer. It covers beer-lambert low and its quantitative applications. It also includes the qualitative applications in different fields of study. Presented at Addis Ababa University, School of medicine, department of medical biochemistry.
Pharmaceuticals: In some pharmaceutical manufacturing processes, minute quantities of a catalyst used in the process (usually a metal) are sometimes present in the final product. By using AAS the amount of catalyst present can be determined.
Atomic absorption spectroscopy (AAS) and atomic emission spectroscopy (AES) is a spectro analytical procedure for the quantitative determination of chemical elements by free atoms in the gaseous state.
Atomic absorption spectroscopy is based on absorption of light by free metallic ions.
In analytical chemistry the technique is used for determining the concentration of a particular element (the analyte) in a sample to be analyzed. AAS can be used to determine over 70 different elements in solution, or directly in solid samples via electrothermal vaporization
Atomic absorption spectrometry (AAS) is an analytical technique that measures the concentrations of elements.
Atomic absorption is so sensitive that it can measure down to parts per billion of a gram (µg dm–3 ) in a sample.
The technique makes use of the wavelengths of light specifically absorbed by an element. They correspond to the energies needed to promote electrons from one energy level to another, higher, energy level.
Atomic absorption spectrometry has many uses in different areas of chemistry.
Clinical analysis : Analysing metals in biological fluids such as blood and urine.
Environmental analysis: Monitoring our environment – eg finding out the levels of various elements in rivers, seawater, drinking water, air, petrol and drinks such as wine, beer and fruit drinks.
The technique makes use of the atomic absorption spectrum of a sample in order to assess the concentration of specific analytes within it. It requires standards with known analyte content to establish the relation between the measured absorbance and the analyte concentration and relies therefore on the [Beer–Lambert law].
The electrons within an atom exist at various energy levels. When the atom is exposed to its own unique wavelength, it can absorb the energy (photons) and electrons move from a ground state to excited states.
The radiant energy absorbed by the electrons is directly related to the transition that occurs during this process.
Furthermore, since the electronic structure of every element is unique, the radiation absorbed represents a unique property of each individual element and it can be measured.
An atomic absorption spectrometer uses these basic principles and applies them in practical quantitative analysis
A typical atomic absorption spectrometer consists of four main components:
Atomization
Light source,
Atomization system,
Monochromator &
Detection system
Atomization can be carried out either by a flame or furnace.
Heat energy is utilized in atomic absorption spectroscopy to convert metallic elements to atomic dissociated vapor.
The temperature should be controlled very carefully for the conversion of atomic vapor.
At too high temperatures, atoms
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
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Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
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Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
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How to Create Map Views in the Odoo 17 ERPCeline George
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Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
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This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
3. FLAME PHOTOMETRY
Introduction
• Flame photometry is also known as Flame emission spectroscopy.
• Flame photometry is based on measurement of intensity of light emitted
when metal is introduced into flame.The wavelength of colour tells us
what the element is and the colour intensity tells us how much of element
is present.
• A photoelectric flame photometer is an instrument used in inorganic
chemical analysis to determine the concentration of certain metal ions
among them sodium,potassium,calcium and lithium.
3
4. PRINCIPLE
- The basic principle upon which Atomic Spectroscopy works is
based on the fact that "Matter absorbs light at the same wavelength at
which it emits light".
- The compounds of the alkali and alkaline earth metals (Group II)
dissociate into atoms when introduced into the flame. Some of these atoms
further get excited to even higher levels. But these atoms are not stable at
higher levels.Hence, these atoms emit radiations when returning back to the
ground state. These radiations generally lie in the visible region of the
spectrum. Each of the alkali and alkaline earth metals has a specific
wavelength.
4
5. Element Emitted wavelength Flame color
Sodium 589 nm Yellow
Potassium 766 nm Violet
Barium 554 nm Lime green
Calcium 622 nm Orange
Lithium 670 nm Red
For certain concentration ranges,
The intensity of the emission is directly proportional to the
number of atoms returning to the ground state. And the light
emitted is in turn proportional to the concentration of the
sample.
5
7. Parts of flame photometer
A simple flame photometer consists of the following basic components:
Source of flame: A Burner in the flame photometer is the source of flame. It can be
maintained in at a constant temperature. The temperature of the flame is one of
critical factors in flame photometry.
Fuel-Oxidant mixture
Temperature (°C)
Natural gas-Air 1700
Propane-Air 1800
Hydrogen-Air 2000
Hydrogen-Oxygen 2650
Acetylene-Air 2300
Acetylene-Oxyen 3200
Acetylene-Nitrous oxide 2700
Cyanogen-Oxygen 4800
7
8. Nebuliser: Nebuliser is used to send homogeneous solution into the flame at a balanced
rate.
Optical system: The optical system consists of convex mirror and convex lens. The convex
mirror transmits the light emitted from the atoms. Convex mirror also helps to focus the
emissions to the lens. The lens helps to focus the light on a point or slit.
Simple colour filters: The reflections from the mirror pass through the slit and reach the
filters. Filters will isolate the wavelength to be measured from that of irrelevant emissions.
Photo-detector: The intensity of radiation emitted by the flame is measured by photo
detector. Here the emitted radiation is converted to an electrical signal with the help of
photo detector. These electrical signals are directly proportional to the intensity of light.
8
9. Events occur in Flame:
The oxidants in flame photometer are mainly air, oxygen or nitrous oxide. The temperature of the
flame depends on the ratio of fuel and oxidant.
The processes occurring during flame photometer analysis are summarized below:
Desolvation: Desolvation involves drying a sample in a solution. The metal particles in the solvent are
dehydrated by the flame and thus solvent is evaporated.
Vaporization: The metal particles in the sample are also dehydrated. This also led to the evaporation of the
the solvent.
Atomization: Atomization is the separation of all atoms in a chemical substance. The metal ions in the
sample are reduced to metal atoms by the flame.
Excitation: The electrostatic force of attraction between the electrons and nucleus of the atom helps them
to absorb a particular amount of energy. The atoms then jump to the higher energy state when excited.
Emission: Since the higher energy state is unstable the atoms jump back to the ground state or low energy
energy state to gain stability. This jumping of atoms emits radiation with characteristic wavelength. The
radiation is measured by the photo detector.
9
10. Applications of flame photometer
1.Flame photometer can be applied both for quantitative and qualitative analysis of elements.
The radiations emitted by the flame photometer are characteristic to particular metal. Hence
with the help of Flame photometer we can detect the presence of any specific element in the
given sample.
2.The presence of some group II elements is critical for soil health. We can determine the
presence of various alkali and alkaline earth metals in soil sample by conducting flame test
and then the soil can be supplied with specific fertiliser.
3.The concentrations of Na+ and K+ ions are very important in the human body for
conducting various metabolic functions. Their concentrations can be determined by diluting
and aspirating blood serum sample into the flame.
4.Soft drinks, fruit juices and alcoholic beverages can also be analysed by using flame
photometry to determine the concentrations of various metals and elements.
10
12. ULTRAVIOLET SPECTROSCOPY
Spectroscopy
• It is the branch of science that deals with the study of interaction of matter with
light. OR
• It is the branch of science that deals with the study of interaction of
electromagnetic radiation with matter.
• The study of molecular or atomic structure of a substance by observation of its
interaction with electromagnetic radiation
• QUANTITATIVELY - For determining the amount of material in a sample
• QUALITATIVELY – For identifying the chemical structure of a sample
12
13. Principles of Spectroscopy
• The principle is based on the measurement of spectrum of a sample containing atoms /
molecules.
• Spectrum is a graph of intensity of absorbed or emitted radiation by sample verses
frequency (v) or wavelength (ʄ).
• Spectrometer is an instrument design to measure the spectrum of a compound.
1. Absorption Spectroscopy:
• An analytical technique which concerns with the measurement of absorption of
electromagnetic radiation.
• e.g. UV (185 - 400 nm) / Visible (400 - 800 nm) Spectroscopy, IR Spectroscopy (0.76 - 15
ʅm)
2. Emission Spectroscopy:
• An analytical technique in which emission (of a particle or radiation) is dispersed
according to some property of the emission & the amount of dispersion is measured.
• e.g. Mass Spectroscopy
13
14. THE LAWS OF SPECTROPHOTOMETRY
There are two very important basic laws and a third one which is a combination of the two.
• LAMBERTS LAW – ABSORBANCE (A) proportional to the PATHLENGTH (l) of the absorbing medium.
• BEERS LAW - ABSORBANCE (A) proportional to the CONCENTRATION (c) of the sample.
• BEER- LAMBERT LAW - ABSORBANCE (A) proportional to c x l
A α cl
A = Ecl (A is a ratio and therefore has no units)
The constant E is called the MOLAR EXTINCTION COEFFICIENT
14
15. IMPORTANCE OF THE BEER LAMBERT LAW
A = Ecl but if E and l are constant
ABSORBANCE α CONCENTRATION and should be linear
relationship Prepare standards of the analyte to be quantified at
known concentrations and measure absorbance at a specified
wavelength. Prepare calibration curve.
From measuring absorbance of sample
Concentration of analyte in sample can be obtained from the
calibration curve
E can be obtained from the slope of the calibration curve for a
given wavelength (l)
15
16. PRINCIPLE OF UV
• UV absorption spectra arise from transition of electron or electron within a molecule or an ion
from a lower to higher electronic energy level and UV emission spectra arise from reverse type
of transition.
• The UV radiation region extends from 10 nm to 400 nm and the visible radiation region extends
from 400 nm to 800 nm.
• Near UV Region: 200 nm to 400 nm
• Far UV Region: below 200 nm
• Far UV spectroscopy is studied under vacuum condition.
• The common solvent used for preparing sample to be analyzed is either ethyl alcohol or
hexane.
16
20. PRINCIPLE
• Infrared spectroscopy (IR) measures the bond vibration frequencies in a molecule and
is used to determine the functional group.
• Just below red in the visible region
• Wavelengths usually 2.5-25 mm
• More common units are wavenumbers, or cm-1 , the reciprocal of the wavelength in
centimeters (104/mm = 4000-400 cm-1 )
• Wavenumbers are proportional to frequency and energy The IR Region
• The IR region is divided into three regions: the near, mid, and far IR. The mid IR
region is of greatest practical use to the organic chemistry
20
22. APPLICATION
• IR is used for determination of impurities.
• It is used for analysis of organic compounds
• IR is used for determination of molecular structure.
22
24. CHROMATOGRAPHY
• It is define as, it is analytical method in which separation of active constituent in complex mixture, and
the mixture was distributed in two phases i.e. stationary phase and mobile phase is known as
chromatography.
• The technique is used for separation, purification, Identification and extraction of compound.
• It is method it can consist of two phases stationary phase and mobile phase.
• Stationary phase is constant phase or column packaging material. Mobile phase is moveable phase.
• The basic principle of chromatography is based on Adsorption and partition chromatography.
- Adsorption chromatography - The affinity of molecules towards stationary phase is known as
Adsorption chromatography.
- Partition chromatography - The molecule can moves in two phases of liquid is known as partition
chromatography.
24
25. HPLC
HPLC is a High Performance liquid Chromatography or High Pressure Liquid Chromatography or High
Priced Liquid Chromatography.
It is column chromatography.
It is Liquid Chromatography.
It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile
compound.
It can mainly divided by two types 1. Normal phase HPLC 2. Reversed Phase HPLC.
It is having a high resolution and separation capacity. It is used as qualitative as well as quantitative
analysis.
High performance liquid chromatography (HPLC) is a chromatographic technique used to separate a
mixture of compounds in analytical chemistry and biochemistry with the purpose of identifying,
quantifying or purifying the individual components of the mixture.
25
26. PRINCIPLE
High Performance Liquid Chromatography [HPLC] is based on adsorption
as well as partition chromatography is depending on the nature of
stationary phase, if stationary phase is solid principle is based on
adsorption chromatography and if stationary phase is liquid principle is
based on partition chromatography.
It is important for determination of volatile and non volatile compounds.
It is important for determination of Retention Time (the time is required ,
after sample injection maximum angle peak reaches to detector)
26
27. TYPES OF HPLC SEPARATIONS
1. Normal Phase: Separation of polar analytes by partitioning onto a polar, bonded
stationary phase.
2. Reversed Phase: Separation of non-polar analytes by partitioning onto a non-polar,
bonded stationary phase.
3. Adsorption: In Between Normal and Reversed. Separation of moderately polar analytes
using adsorption onto a pure stationary phase (e.g. alumina or silica)
4. Ion Chromatography: Separation of organic and inorganic ions by their partitioning
onto ionic stationary phases bonded to a solid support.
5. Size Exclusion Chromatography: Separation of large molecules based in the paths they
take through a “maze” of tunnels in the stationary phase.
27
28. INSTRUMENTATION
o Solvent storage bottle
o Gradient controller and mixing unit
o De-gassing of solvents
o Pump
o Pressure gauge
o Pre-column
o Sample introduction system
o Column
o Detector
o Recorder
28
29. DETECTORS
• Absorbance (UV/Vis and PDA)
• Refractive index (detects the change in turbidity)
• Fluorescence (if the analyte is fluorescent)
• Electrochemical (measures current flowing through a pair
of electrodes, on which a potential difference is imposed,
due to oxidation or reduction of solute)
• Conductivity (for ions)
• Light scattering
• Mass spectrometry (HPLC-MS)
29
Types of pumps used in HPLC
• DISPLACEMENT PUMPS
• RECIPROCATING PUMPS
• PNEUMATIC PUMP
HPLC Columns Used in Analysis
• Normal Phase Columns.
• Reverse Phase Columns.
• Ion Exchange Columns.
• Size Exclusion Columns
30. APPLICATION
o Drug Discovery
o Clinical Analysis
o Forensic Chemistry
o Drug Metabolism study
o Environmental chemistry
o Diagnostic studies
o Determination of Green Florescent Protein
o Structural Determination
30