The document discusses primary and secondary metabolism in plants. Primary metabolism produces primary metabolites like carbohydrates, proteins, lipids and nucleic acids that are essential for growth and survival. The pathways for primary metabolism are essentially the same across organisms. Secondary metabolism produces secondary metabolites that are not essential for growth but provide functions like defense against predators through toxicity, attracting pollinators or dispersing seeds. Secondary metabolites have more limited distribution between taxa and their roles are often unclear.
Quality control of Drugs of Natural Origin. PHARMACognosy & Phytochemistry-I ...Ms. Pooja Bhandare
Quality control of Drugs of Natural Origin PHARMACognosy & Phytochemistry-I (BP405T)Unit-I Part-3.
CONTENTS
Adulteration
Evaluation of adulteration
Morphological / Organoleptic evaluation
Microscopic evaluation
Quantitative evaluation
Physical evaluation
Chemical evaluation
Biological evaluation
Adulteration is of two types:
Indirect or Unintentional adulteration
Direct or Intentional adulteration
Intentional adulteration may be due to the following reasons
adulteration using manufactured substances
substitution using inferior commercial varieties
substitution using exhausted drugs
substitution of superficially similar inferior natural substance
adulteration using the vegetative part of the same plant
addition of toxic materials
adulteration of powders
addition of synthetic principles
Evaluation of Crude Drugs
1. ORGANOLEPTIC EVALUATION
2. MICROSCOPICAL EVALUATION
Stomatal index Vein-islet number
Veinlet termination number
Palisade ratio
Quantitative Microscopy (Lycopodium Spore Method)
3.CHEMICAL EVALUATION
4. Physical Evaluation
I. Solubility
II. Optical Rotation
III. Refractive Index
III. Specific Gravity
IV Viscosity
V. Melting Point
VI. Moisture Content
VII. Ultraviolet Light
VIII. Ash Values
Total ash
Acid-insoluble ash
The water-soluble ash
IX. Extractive Values
X. Foreign Organic Matters
5. BIOLOGICAL EVALUATION
Toxicity
Oxytocic activity
Microbiological assays
Microbiological Assay of Vitamin & Amino acid Assessment of a New Antibiotic...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T) Unit-IV Part-3
Microbiological Assay of Vitamin & Amino acid Assessment of a New Antibiotic: Introduction:
Principle
Microbiological Assay of Cynocobalamin (Vitamin B12):
Tritrimetric Method.
Turbidimetric Method.
Preparation of Standard Cynocobalmine stock solution:
Preparation of Basal Medium Stock Solution:
Test Solution of the material to be assayed Preparation of inoculum: Procedure of Titrimetric method: Turbidimetric Method: Microbiological assay of Amino acids. Assessment of a New Antibiotic.
Introduction:
MIC of an antibiotic is tested either by one of the following ways,
Liquid Dilution Method.
Solid Dilution Method
VIRUS PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-IIIPart-2Study of morphology, ...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-IIIPart-2Study of morphology, classification, reproduction/replication and cultivation of Virus. Introduction, Def General characteristics of Viruses: small size characteristic shapes, obligate intracellular parasites no built-in metabolic machinery no ribosomes
only one type of nucleic acid
do not grow in size. Morphology of Virus: Helical, Polyhedral (Icosahedral) Viral Envelop, Complex virus, Classification of virus. Viral Replication LIFE CYCLE OF BACTIRIOPHAGES Lytic cycle: Attachment, Penetration, Biosynthesis, Maturation and Release of progeny Phage Particles. The Lysogenic Cycle, Cultivation of virus : Animal inoculation, Embryonated eggs or chick embryo method and Tissue culture or cell culture: Organ cultures Explant culture and Cell culture. Types of cell culture
1.Primary cell culture: 2. Diploid cell culture (Semi-continuous cell lines):3. Heteroploid cultures (Continuous cell lines):
MULTIPLICATION OF HUMAN VIRUS:1. Attachment of Viral Particles 2. Penetration 3. Uncoating 4. Replication Of Viral Nucleic Acids And Translation Of The Genome 5) Maturation Or Assembly Of Virions. ) 6. Release Of Virions Into The Surrounding Environment
According to the PCI Syllabus ,B Pharm V Sem Sub : Pharmacognosy and Phytochemistry II
It contains the general introduction of metabolic Pathways ,Metabolites and How primary metabolites are linked with secondary metabolites
PHARMACOGNOSY & Phytochemistry-I (BP405T)Unit-IIPart-2.FACTORS AFFECTING CULTIVATION
1. Altitude
2.Temperature
3. Rainfall
4. Day Length and Day Light
5. Soil
6. Soil Fertility
7. Fertilizers and Manures
a) Chemical fertilizers
(b) Manures
(c) Biofertilizers
8. Pests and Pests Control
a. Microbes
b) Insects
C) Non insect pests
d) Weeds
9. Other Factors that Affect the Cultivated Plants
a. Air Pollution
b. Herbicide
THIS SLIDE CONTAIN ABOUT QUALITATIVE TEST, STRUCTURE AND USES OF DIFFERENT CARBONYL COMPOUNDS LIKE FORMALDEHYDE, PARALDEHYDE, ACETONE, CHLORAL HYDRATE, HEXAMINE, BENZALDEHYDE, VANILIN AND CINNAMALDEHYDE
Quality control of Drugs of Natural Origin. PHARMACognosy & Phytochemistry-I ...Ms. Pooja Bhandare
Quality control of Drugs of Natural Origin PHARMACognosy & Phytochemistry-I (BP405T)Unit-I Part-3.
CONTENTS
Adulteration
Evaluation of adulteration
Morphological / Organoleptic evaluation
Microscopic evaluation
Quantitative evaluation
Physical evaluation
Chemical evaluation
Biological evaluation
Adulteration is of two types:
Indirect or Unintentional adulteration
Direct or Intentional adulteration
Intentional adulteration may be due to the following reasons
adulteration using manufactured substances
substitution using inferior commercial varieties
substitution using exhausted drugs
substitution of superficially similar inferior natural substance
adulteration using the vegetative part of the same plant
addition of toxic materials
adulteration of powders
addition of synthetic principles
Evaluation of Crude Drugs
1. ORGANOLEPTIC EVALUATION
2. MICROSCOPICAL EVALUATION
Stomatal index Vein-islet number
Veinlet termination number
Palisade ratio
Quantitative Microscopy (Lycopodium Spore Method)
3.CHEMICAL EVALUATION
4. Physical Evaluation
I. Solubility
II. Optical Rotation
III. Refractive Index
III. Specific Gravity
IV Viscosity
V. Melting Point
VI. Moisture Content
VII. Ultraviolet Light
VIII. Ash Values
Total ash
Acid-insoluble ash
The water-soluble ash
IX. Extractive Values
X. Foreign Organic Matters
5. BIOLOGICAL EVALUATION
Toxicity
Oxytocic activity
Microbiological assays
Microbiological Assay of Vitamin & Amino acid Assessment of a New Antibiotic...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T) Unit-IV Part-3
Microbiological Assay of Vitamin & Amino acid Assessment of a New Antibiotic: Introduction:
Principle
Microbiological Assay of Cynocobalamin (Vitamin B12):
Tritrimetric Method.
Turbidimetric Method.
Preparation of Standard Cynocobalmine stock solution:
Preparation of Basal Medium Stock Solution:
Test Solution of the material to be assayed Preparation of inoculum: Procedure of Titrimetric method: Turbidimetric Method: Microbiological assay of Amino acids. Assessment of a New Antibiotic.
Introduction:
MIC of an antibiotic is tested either by one of the following ways,
Liquid Dilution Method.
Solid Dilution Method
VIRUS PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-IIIPart-2Study of morphology, ...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-IIIPart-2Study of morphology, classification, reproduction/replication and cultivation of Virus. Introduction, Def General characteristics of Viruses: small size characteristic shapes, obligate intracellular parasites no built-in metabolic machinery no ribosomes
only one type of nucleic acid
do not grow in size. Morphology of Virus: Helical, Polyhedral (Icosahedral) Viral Envelop, Complex virus, Classification of virus. Viral Replication LIFE CYCLE OF BACTIRIOPHAGES Lytic cycle: Attachment, Penetration, Biosynthesis, Maturation and Release of progeny Phage Particles. The Lysogenic Cycle, Cultivation of virus : Animal inoculation, Embryonated eggs or chick embryo method and Tissue culture or cell culture: Organ cultures Explant culture and Cell culture. Types of cell culture
1.Primary cell culture: 2. Diploid cell culture (Semi-continuous cell lines):3. Heteroploid cultures (Continuous cell lines):
MULTIPLICATION OF HUMAN VIRUS:1. Attachment of Viral Particles 2. Penetration 3. Uncoating 4. Replication Of Viral Nucleic Acids And Translation Of The Genome 5) Maturation Or Assembly Of Virions. ) 6. Release Of Virions Into The Surrounding Environment
According to the PCI Syllabus ,B Pharm V Sem Sub : Pharmacognosy and Phytochemistry II
It contains the general introduction of metabolic Pathways ,Metabolites and How primary metabolites are linked with secondary metabolites
PHARMACOGNOSY & Phytochemistry-I (BP405T)Unit-IIPart-2.FACTORS AFFECTING CULTIVATION
1. Altitude
2.Temperature
3. Rainfall
4. Day Length and Day Light
5. Soil
6. Soil Fertility
7. Fertilizers and Manures
a) Chemical fertilizers
(b) Manures
(c) Biofertilizers
8. Pests and Pests Control
a. Microbes
b) Insects
C) Non insect pests
d) Weeds
9. Other Factors that Affect the Cultivated Plants
a. Air Pollution
b. Herbicide
THIS SLIDE CONTAIN ABOUT QUALITATIVE TEST, STRUCTURE AND USES OF DIFFERENT CARBONYL COMPOUNDS LIKE FORMALDEHYDE, PARALDEHYDE, ACETONE, CHLORAL HYDRATE, HEXAMINE, BENZALDEHYDE, VANILIN AND CINNAMALDEHYDE
Preservation of pharmaceutical products using antimicrobial agents. PHARMACEU...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-VPart-3
Preservation of pharmaceutical products using antimicrobial agents.
Introduction. Ideal Properties of Preservatives:
Antimicrobial Chemical Preservatives
Development of a Preservative System.
Factors affecting efficacy of a preservative: 1. Interaction With components of the formulation
2. Properties of the Preservatives:
3) Effect of Containers.
4) Type of microbes:
5) Influence of pH:
Challenge Test: Efficacy Test of Preservative : Medium used, Choice of test organism:
Preparation of the inoculum:
Procedure:
Interpretation of Results:
PHARMACEUTICAL MICROBIOLOGY (BP303T) Unit-III Part-1 Study of morphology, cla...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-IIIPart-1Study of morphology, classification, reproduction/replication and cultivation of fungi, Introduction fungi. Morphological Characteristics of fungi, CLASSIFICATION: Depending on cell morphology, fungi can be divided into 4 classes:
Moulds Yeasts ,Yeast like fungi and
Dimorphic fungi
Depending on their sexual spores formation fungi are divided into 4 classes:
Zygomycetes Ascomycetes
Basidiomycetes Dueteromycetes
Reproduction and sporulation;Vegetative, Asexual
and Sexual
Vegetative reproduction: Fragmentation ,Fission, budding, Sclerotia Rhizomorphs
Asexual reproduction: Zoospores
Sporangiospore, Conidia
Oidia Uredospores ,Basidiospores
Sexual reproduction:Planogametic copulation: Isogamy Heterogamy
Gametangial contact
Gametangial copulation Spermatization Somatogamy CULTIVATION OF FUNGI: Brain Heart Infusion (BHT) agar
Czapek’s agar
Mycobiotic agar Inhibitory mold agar (IMA)
Potato dextrose agar
Sabouraud’s dextrose agar (SDA):
Sabouraud’s heart infusion (SABHI) agar
Potato Flake agar
Potato dextrose-yeast extract agar (PDYA)
. Cornmeal agar
Malt extract agar (MEA)
Evaluation of Bactericidal and Bacteriostatic (Disinfectant). PHARMACEUTICAL ...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-III Part-5 Evaluation of Bactericidal and Bacteriostatic (Disinfectant). The common methods used for evaluation of a disinfectant are as follows,
Tube Dilution Method.
Agar Plate Method.
Filter Paper & Cup Plate Method.
Ditch-Plate Method.
Phenol Coefficient Method.
The official phenol coefficient tests include,
Rideal-Walker Test (RW Test).
Chick-Martin Test.
United States FDA Test for Phenol Coefficient. (FDA Test)
The US Association of Official Agricultural Chemists Test (FDA Test)
A. Rideal-Walker Test:
Kelsey Sykes Method
Crude drugs are plant, animal or their parts which after collection are subjected only to drying or making them into transverse/ longitudinal slices pieces or peeling them in some cases. They exist in natural form.
Crude drugs may be derived from various natural sources like Plants, Animals, Minerals, Marine and Micro-organisms etc
: In this video the viewers will come to know about Castor oil that is one of the Lipid containing crude drugs obtained from the plant sources Castor oil is the fixed oil obtained by cold expression of the seeds of Ricinus communis Linn., belonging to family Euphorbiaceae. This drug becomes important since it is obtained from seed source. Here the synonyms, biological sources (scientific names & Family), geographical sources (what are the countries where it can be collected), chemical constituents, identification tests and uses has been discussed in brief.
Portion explained:
1. Synonyms of Castor oil
2. Biological Sources of Castor oil
3. Geographical Sources of Castor oil
4. Preparation of Castor oil
5. Description of Castor oil
6. Chemical Constituents of Castor oil
7. Chemical Test of Castor oil
8. Uses of Castor oil
Preservation of pharmaceutical products using antimicrobial agents. PHARMACEU...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-VPart-3
Preservation of pharmaceutical products using antimicrobial agents.
Introduction. Ideal Properties of Preservatives:
Antimicrobial Chemical Preservatives
Development of a Preservative System.
Factors affecting efficacy of a preservative: 1. Interaction With components of the formulation
2. Properties of the Preservatives:
3) Effect of Containers.
4) Type of microbes:
5) Influence of pH:
Challenge Test: Efficacy Test of Preservative : Medium used, Choice of test organism:
Preparation of the inoculum:
Procedure:
Interpretation of Results:
PHARMACEUTICAL MICROBIOLOGY (BP303T) Unit-III Part-1 Study of morphology, cla...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-IIIPart-1Study of morphology, classification, reproduction/replication and cultivation of fungi, Introduction fungi. Morphological Characteristics of fungi, CLASSIFICATION: Depending on cell morphology, fungi can be divided into 4 classes:
Moulds Yeasts ,Yeast like fungi and
Dimorphic fungi
Depending on their sexual spores formation fungi are divided into 4 classes:
Zygomycetes Ascomycetes
Basidiomycetes Dueteromycetes
Reproduction and sporulation;Vegetative, Asexual
and Sexual
Vegetative reproduction: Fragmentation ,Fission, budding, Sclerotia Rhizomorphs
Asexual reproduction: Zoospores
Sporangiospore, Conidia
Oidia Uredospores ,Basidiospores
Sexual reproduction:Planogametic copulation: Isogamy Heterogamy
Gametangial contact
Gametangial copulation Spermatization Somatogamy CULTIVATION OF FUNGI: Brain Heart Infusion (BHT) agar
Czapek’s agar
Mycobiotic agar Inhibitory mold agar (IMA)
Potato dextrose agar
Sabouraud’s dextrose agar (SDA):
Sabouraud’s heart infusion (SABHI) agar
Potato Flake agar
Potato dextrose-yeast extract agar (PDYA)
. Cornmeal agar
Malt extract agar (MEA)
Evaluation of Bactericidal and Bacteriostatic (Disinfectant). PHARMACEUTICAL ...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-III Part-5 Evaluation of Bactericidal and Bacteriostatic (Disinfectant). The common methods used for evaluation of a disinfectant are as follows,
Tube Dilution Method.
Agar Plate Method.
Filter Paper & Cup Plate Method.
Ditch-Plate Method.
Phenol Coefficient Method.
The official phenol coefficient tests include,
Rideal-Walker Test (RW Test).
Chick-Martin Test.
United States FDA Test for Phenol Coefficient. (FDA Test)
The US Association of Official Agricultural Chemists Test (FDA Test)
A. Rideal-Walker Test:
Kelsey Sykes Method
Crude drugs are plant, animal or their parts which after collection are subjected only to drying or making them into transverse/ longitudinal slices pieces or peeling them in some cases. They exist in natural form.
Crude drugs may be derived from various natural sources like Plants, Animals, Minerals, Marine and Micro-organisms etc
: In this video the viewers will come to know about Castor oil that is one of the Lipid containing crude drugs obtained from the plant sources Castor oil is the fixed oil obtained by cold expression of the seeds of Ricinus communis Linn., belonging to family Euphorbiaceae. This drug becomes important since it is obtained from seed source. Here the synonyms, biological sources (scientific names & Family), geographical sources (what are the countries where it can be collected), chemical constituents, identification tests and uses has been discussed in brief.
Portion explained:
1. Synonyms of Castor oil
2. Biological Sources of Castor oil
3. Geographical Sources of Castor oil
4. Preparation of Castor oil
5. Description of Castor oil
6. Chemical Constituents of Castor oil
7. Chemical Test of Castor oil
8. Uses of Castor oil
Pharmacognosy and phytochemistry- II/ semester V/ Unit I/Basic metabolic pathway/ Primary metabolites/ secondary metabolites/ formation secondary metabolites/ Formation of amino acid / role of enzyme/ role of coenzyme
"Bacterial metabolism: Fueling life's processes in tiny powerhouses."
Use of bacterial metabolism in biotechnology, biofuels, and other industries
Examples of how bacterial metabolism is harnessed for beneficial purposes
"Metabolism: the sum of chemical reactions in an organism, supporting growth, energy production, and vital functions."
"Bacterial Metabolism and Life: Pervading every aspect of life, shaping ecosystems, and influencing our world."
Bacterial metabolism refers to the collective chemical reactions and processes that occur within bacterial cells, enabling them to maintain life, grow, and reproduce. These metabolic activities involve a complex network of biochemical pathways that facilitate the conversion of nutrients into energy, biomolecules, and essential compounds necessary for bacterial survival.
Metabolic processes in bacteria include catabolic pathways that break down complex molecules (such as sugars) to release energy and anabolic pathways that build complex molecules (such as proteins, nucleic acids) using energy. Bacteria utilize various metabolic strategies based on their energy and carbon sources, including aerobic and anaerobic respiration, fermentation, and photosynthesis in photosynthetic bacteria.
The primary goals of bacterial metabolism are to obtain energy, synthesize necessary cellular components, regulate chemical processes, and adapt to changing environmental conditions. The understanding of bacterial metabolism is crucial for various fields, including medicine, agriculture, biotechnology, and environmental science, as it allows us to develop strategies to combat harmful bacteria, harness their metabolic capabilities for beneficial applications, and study their role in ecological systems.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
2. • Despite the extremely varied characteristics of living organisms, the
pathways for generally modifying and synthesizing carbohydrates,
proteins, fats, and nucleic acids are found to be essentially the same
in all organisms, apart from minor variations.
• These processes demonstrate the fundamental unity of all living
matter, and are collectively described as ‘primary metabolism’, with
the compounds involved in the pathways being termed ‘primary
metabolites’.
• Thus degradation of carbohydrates and sugars generally proceeds
via the well-characterized pathways known as glycolysis and the
Krebs/citric acid/tricarboxylic acid cycle, which release energy from
the organic compounds by oxidative reactions. Oxidation of fatty
acids from fats by the sequence called β-oxidation also provides
energy.
3. • In contrast to these primary metabolic pathways, which
synthesize, degrade, and generally interconvert compounds
commonly encountered in all organisms, there also exists an
area of metabolism concerned with compounds which have a
much more limited distribution in nature.
• Such compounds, called ‘secondary metabolites’, are found in
only specific organisms, or groups of organisms, and are an
expression of the individuality of species. Secondary
metabolites are not necessarily produced under all conditions,
and in the vast majority of cases the function of these
compounds and their benefit to the organism is not clear.
4. • Some are undoubtedly produced for easily appreciated
reasons, for example,
• as toxic materials providing defence against predators
• as volatile attractants towards the same or other species, or
as colouring agents to attract or warn other species, but it is
logical to assume that all do play some vital role for the well-
being of the producer.
• It is this area of ‘secondary metabolism’ that provides most of
the pharmacologically active natural products.
5. • PRIMARY METABOLITES: Molecules that are essential for
growth and development of an organism. Examples:
• 1.Carbohydrates 2.Proteins 3.Lipids
• 4.Nucleic acids 5.Hormones
• SECONDARY METABOLITES: Molecules that are not
essential for growth and development of an organism and are
biosynthetically derived from primary metabolites. They are
more limited in distribution being found usually in specific
families.
• 1. Terpenoids 2. Nitrogen containing compounds
• 3. Phenolics
6. Primary plant metabolites are considered as basic plant
constituents.
They are simple in structure & do not have any biological or
pharmacological activity associated with them.
They are utilized by plants for building up their body &
responsible for physiological functioning of the plant body.
There are distinct pathways and chain of reactions through
which they are synthesized or produced known as basic
metabolic pathways.
Kreb cycle, citric acid, glycolysis for degradation of
carbohydrates, oxidation of fatty acids by beta oxidation are
primary metabolic raections.
7. • Secondary metabolites are biosynthesized from primary
metabolites.
• These are potent in action & associated with marked
pharmacological actions on human body known as active
constituents.
• The quantity of active constituents ( secondary metabolites)
determines the intrinsic important role in the process of
biosynthesis.
• Main classes are polyketides, fatty acids, terpenoids, steroids,
alkaloids and specialize amino acids and carbohydrates.
8. • Secondary metabolites are those metabolites which are often
produced in a phase of subsequent to growth, have no
function in growth, are produced by certain restricted
taxonomic groups of microorganisms, have unusual
chemicals structures, and are often formed as mixtures of
closely related members of a chemical family.
• The simplest definition of secondary products is that they are
not generally included in standard metabolic charts.
• Plants produce a large, diverse array of organic compounds
that appear to have no direct function in growth, development,
photosynthesis, respiration, solute transport, translocation,
protein synthesis, nutrient assimilation differentiation, or the
formation of carbohydrates called as secondary metabolites.
9. • Thus small organic molecules produced by an organism that
are not essential for their growth, development and
reproduction are called secondary metabolites.
• They may include pharmaceuticals, flavours, fragrance, food
additives, feedstock etc. Possibly over 250,000 secondary
metabolites in plants.
• Classified based on common biosynthetic pathways where a
chemical is derived.
• Four major classes: Alkaloids, glycosides, phenolics,
terpenoids
10. • Plant secondary metabolites are a diverse group of molecules
that are involved in the adaptation of plants to their
environment but are not part of the primary biochemical
pathways of cell growth and reproduction.
• These compounds play many important rules in plant life
such as involved in defense against herbivores and
pathogens, regulation of symbiosis, control of seed
germination, and chemical inhibition of competing plant
species (allelopathy), and therefore are an integral part of the
interactions of species in plant and animal communities and
the adaptation of plants to their environment.
11. • Higher plants synthesize these wide variety of low molecular
weight compounds called secondary metabolites in addition to
the essential primary metabolites.
• These offer protection against pests, they act as attractants
and as the plant’s own hormones. Chemically meant to
protect plants from the attacks by predators, pathogens, or
competitors.
• Attract pollinators as seed dispersal agent
• Important for abiotic stress
• Medicine
• Industrial additives
12. • In addition, plant secondary metabolites are also
associated with improved nutritive value and may have
beneficial effects on animal health.
• Growing interest in the potential health-promoting
effects of plant secondary metabolites in human foods
has prompted research on their potential to prevent or
treat cancer, circulatory disease, and viral infection and
many more life threatning diseases.
13. • A metabolic intermediate or product, found as a differentiation
product in restricted taxonomic groups, not essential to growth
and the life of the producing organism, and biosynthesis from
one or more general metabolites by a wider variety of
pathways than is available in general metabolism.
• They have a wide range of chemical structures and biological
activities. They are derived by unique biosynthetic pathways
from primary metabolites and intermediates.
• These biochemical pathways are not necessary for growth or
reproduction of an organism, but which can be demonstrated
genetically, physiologically or biochemically.
14. • Plants produce an amazing diversity of low molecular weight
compounds.
• The ability to synthesize secondary metabolites has been
selected through the course of evolution in different plant
lineage when such compounds address specific needs.
• Floral scent volatiles and pigments have evolved to attract
insect pollinators and thus enhance fertilization.
• To synthesize toxic chemical has evolved to ward off
pathogens and herbivores or to suppress the growth of
neighboring plants.
15. • Chemicals found in fruits prevent spoilage and act as signals
(in the form of color, aroma, and flavor) of the presence of
potential rewards (sugars, vitamins and flavor) for animals
that eat the fruit and thereby help to disperse the seeds.
• Other chemicals serve cellular functions that are unique to the
particular plant in which they occur (e.g. resistance to salt or
drought).
16. • Primary metabolism
• The biological reactions are essential to maintain life in living
organisms and are known as primary metabolism.
• Plant convert sunlight energy to chemical energy, such as
ATP, NADPH, by the mediation of chlorophyll in chloroplasts
and synthesize sugars and starch from CO2 by using ATP
and NADPH+.
• These carbohydrates are stored and used for differentiation
and formation of plant tissues.
17. • All organisms need to transform and interconvert a vast
number of organic compounds to enable them to live, grow
and reproduce.
• All organisms need to provide themselves with energy in the
form of ATP, and a supply of building blocks to construct their
own tissues.
• An integrated network of enzyme-mediated and carefully
regulated chemical reactions in used for this purpose,
collectively referred to as intermediary metabolism, and the
pathways involved are termed metabolic pathway.
• These processes demonstrate the fundamental unity of all
living matter, and are collectively described as primary
metabolism, with the compounds involved in pathways being
termed primary metabolites.
18. • The pathways for modifying and synthesizing carbohydrates,
proteins, fats and nucleic acid are found to be same in all
organisms, apart from minor variations.
• These processes demonstrate the fundamental unity of all
living matter and are collectively described as primary
metabolism with the compounds involved in pathways are
termed as primary metabolites.
19. • Secondary metabolism
• The metabolism which are not directly related to maintaining
life, are known as secondary metabolism.
• The products formed by secondary metabolism are called
secondary metabolites.
• Secondary metabolite play a role in reinforcement of tissue
and body (e.g. cellulose, lignin, suberin), protection against
insects, diseases, and plant regulation (plant hormones).
20. • The compounds which synthesized from the secondary
metabolisms are so-called secondary metabolites.
• Secondary metabolites are formed in only specific organisms,
or groups of organisms, and are expression of the individuality
of species.
• Secondary metabolites are not necessarily produced under all
conditions, and in the vast majority of cases the function of
these compounds and their benefit to the organism is not yet
known.
• It is this area of secondary metabolism that provides most of
the pharmacologically active natural products.
21. • They are classified on the basis of:
• 1) chemical structure
• 2) chemical composition
• 3) solubility in various solvents
• 4) pathways by which they are synthesized
22. • A simple classification of secondary metabolites includes
three main groups:
• 1) the terpenes- made from mevalonic acid, composed almost
entirely of carbon and hygrogen
• 2) phenolics – made from simple sugars, containing benzene
rings, hydrogen and oxygen
• 3) nitrogen-containing compounds – extremely diverse, may
also contain sulphur
23. • To make such compounds plants utilize very specific
enzymes each of which catalyzes a specific metabolic
reaction.
These enzymes are proteins called as organic catalyst coded
by specific genes in the plants DNA.
These series of enzymatically catalyzed reactions in a well
defined sequence of step is termed as a metabolic pathway.