Wide hybridization is a technique used to transfer agriculturally important traits from alien species to cultivated plants. It allows for greater genetic variability but can be hampered by issues like poor crossability and hybrid sterility. These barriers have been overcome through techniques like the use of growth hormones, improved culture conditions, chromosome doubling, and bridge crosses. Alien addition lines carry one chromosome pair from another species in addition to the parent species' normal chromosomes. They allow for the transfer of traits like disease resistance while limiting the introduction of undesirable genes. Alien addition lines have been developed in several important crop species like wheat and tobacco.

1. ALIEN ADDITION LINE
• SUBMITTED TO:
• DR. K.K. PANIGRAHI
• ASST. PROFESSOR IN PLANT BREEDING AND GENETICS
SUBMITTED BY:
ABINASH BHANJA
ADM.NO:48C/15
2ND YR BSC (AG)

2. ALIEN ADDITION LINE
• The transfer of agriculturally important traits from alien species to
cultigens by interspecific and intergeneric crosses, known as wide
hybridization.
• Wide hybridization is a valuable tool for creating genetic variability
in plant breeding by broadening the germplasm base of the
cultigens exploitation of wild relatives of the crop plants.
• It is often hampered because of poor crossability, early embryo
abortion, hybrid seed inviability, hybrid seedling lethality, and
hybrid sterility due to low chromosome pairing.

3. THESE BARRIERS HAVE BEEN OVERCOME BY
(1) The Assemblage Of Diverse Germplasm;
(2) Application Of Growth Hormones To Reduce Embryo Abortion;
(3) Improved Culture Conditions;
(4) Restoration Of The Seed Fertility By Doubling The Chromosomes Of
Sterile F1 Hybrids; And
(5) Utilization Of Bridge Crosses Where Direct Crosses Are Not Possible.

4. • These alien addition lines carries one chromosome pair
from a different species in addition to the normal
somatic chromosome complement of the parent species.
• When only one chromosome from another species is
present ,is known as alien addition monosome.
• The main purpose of alien addition is the transfer of
disease resistance from related wild species.
• E.G. Transfer of mosaic resistance from Nicotiana
glutinosa to N. Tabacum.

7. • The alien addition lines have been developed in case of
wheat, oats, tobacco and several other species.
• Alien addition lines are of still agricultural importance
since the alien chromosomes generally caries many
undesirable genes.
• Production of alien addition lines in wheat :

9. THANK YOU