very nice and beautiful pictures of the day

1. Extraction methods of
fermentation broth
Manasi chauhan
Roll 127
MSC –ll Botany

2. Solvent extraction method
• It is process in which compounds ate separated based on their
relative solubility.
• Factors to be considered
• Purity
• During dissolution ,annalyte
• Has too be made less hydrophilic and more hydrophobic

3. Choices of solvent : liqid-liquid extraction
• Osmotic shock
• Alkali treatment
• Enzymatic treatment eg.
• Lysozyme
• D=C%C○

4. Batch distillation plant with tray or perforated
plate column

5. Podbielniak extractor
The force is used in this
machine is centrifugal
force
Mainly used in industry for
e.g. penicillin extraction

7. Cocurrent and countercurrent flow extraction
system
In concurrent flow
the solvent is
added after each
mixer separator
In countercurrent
flow one time
solvent added.

9. Chromatography
• Adsorption chromatography
• Ion – exchange
chromatography
• Gel permeation
chromatography
• High performance liquid
chromatography

10. Removal of volatile products
Pervaporation
module Is there in
this permeable
membrane is present
which works
according to its
affinity

11. Factors affecting chromatography
• Choice of the adsorbent
• Selection of the solvent for the mixture
• The rate of flow of the solvent
• The temperature of the system
• The column of the height for the procedure

12. Gel Filtration
• A type of size exclusion chromatography can be used to
either fractionate molecule and complex in a sample into
fraction with a particular size from the sample or
combination .

13. Factors affecting
• Fraction range
• Size exclusion limit
• Flow rate
• Sample viscosity

14. Reference
• https://www.sciencedirect.com/topics/immunology-and-
microbiology/gel-filtration
https://www.bio-rad.com/featured/en/gel-filtration-
chromatography.html

15. Thank you