TLC-V1.pptx
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The separation technique-Thin Layer Chromatography and its methods. Other separating methods-Soxlet, solvent extraction
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Chromatography is a technique used to separate mixtures into individual components. It was first invented in 1906 by Mikhail Tswett who separated chlorophyll and xanthophyll. There are two main types of chromatography: partition chromatography and adsorption chromatography. Paper chromatography uses a paper strip as the stationary phase, with samples applied as spots and developed with a mobile phase solvent. Thin layer chromatography uses coated plates as the stationary phase. Gas chromatography uses gases as the mobile phase to separate components through a column at elevated temperatures. High performance liquid chromatography applies high pressure to the mobile phase to rapidly separate components through smaller stationary phase particles.
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Chromatography is a technique used to separate mixtures into individual components. It works by distributing components between a stationary and mobile phase based on differences in how strongly each component interacts with the phases. The main types are adsorption, partition, ion exchange, size exclusion, and affinity chromatography. Chromatography is widely used in industries like pharmaceuticals, food, chemicals, and molecular biology to analyze, identify, purify, and quantify mixtures and components.
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