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1. Edible vaccine
Manasi chauhan
Roll 127
MSC-ll Botany

2. Edible vaccine from plant
• Edible vaccines are the transgenic plant and animal based
production of or those contain agent that trigger on animals immune
response.
• 1989 the effort to produce by Hiatt and coworkers
• In the early 1990s Charles j . Arntzen then at Texas A&M University
conceived of a way to solve many Of the bar problem that bar
vaccine from reaching all too many children in developing nations.
• In 1996 in tobacco plant made as vaccine for Norwalk virus.
• Eg, Hepatitis B , diarrhea

3. Production of vaccine
• Propagation
• Isolation
• Purification
• Formulation
• Methods to make edible
vaccines
1. Indirect method-
mediated by vector
2. Direct method – gene
delivery method eg
biolistic gun or gene gun

4. How edible vaccine works

5. Banana(Musa sapientum)
• Banana is still considered an ideal source of vaccine as well as grown in
both tropics and subtropical regions.
• They are eaten raw hence the protein they contain is not denatured,
which makes them ideal candidate for edible vaccine.
• It reports that banana plant express HBsAg and especially leaves contain
antigen.
• It is edible vaccine candidate for diarrhea.
• It is a vaccine for enteritis caused by Ecoli. strain

6. Lettuce ( Lactuca saliva)
• Lettuce is one of the indispensable vegetables for salads that people
consume in daily life.
• Trials are underway to develop an HBV vaccine in lettuce.
• Most important the expression of measles virus In MVH in lettuce .
• In addition, the heat – stable B- subunits of cholera toxin was
expressed in lettuce.

7. Potato ( Solanum tuberosum)
• Potatoes are excellent source of high quality protein and of lysine
amino acids.
• It has been proven that enteric pathogens such as ETEC LT- B and
Norwalk virus capsid protein can be successfully expressed in
Potatoes tubers .
• Apart from this vaccine are also developed for enteric pathogens
such as cholera and rotavirus.

8. Tomatoes ( Solanum lucopersicum )
• The tomato plant was first used in the development of an edible
rabies vaccine.
• To express the epitope for respiratory syncytial virus along with
Hepatitis E virus, Yersina, DPT endotoxin and synthetic HBV and HIV
antigen.
• It is engineered to express the gene for glycoprotein (G- protein) that
coats the outer surface of rabies virus to develop edible vaccine .

9. Tobacco ( Nicotina tabacum)
• Model plant in biotechnology.
• In type I diabetes or insulin- dependent
is a disease caused by autoimmune
destruction of insulin secreting beta
cells in pancreas, and transgenic plant
tobacco with the gene encoding
GAD67 has been developed as an
edible vaccine mechanism.
• Tetanus, Goucher , malaria, Ebola , HIV
, blue tounge , rabies, dengue fever
and many has potential as an edible
vaccine.

11. Advantages of edible vaccine
• Cost-effective
• Needless
• Convenient
• Safe
• Especially for fatal illness of poultry disease
• Easy for storing and administrating the vaccine

12. Reference
• https://medwinpublishers.com/VIJ/plants-that-can-be-used-as-plant-based-
edible-vaccines-current-situation-and-recent-developments.pdf
• Kurup VM, Thomas J. Edible Vaccines: Promises and Challenges. Mol
Biotechnol. 2020 Feb;62(2):79-90. doi: 10.1007/s12033-019-00222-1. PMID:
31758488; PMCID: PMC7090473.

13. Thank you