Plasma cell disorders is a difficult topic where most residents and students confuse with regarding to differentiating between various types of para-proteinemias or plasma cell dyscrasias. This simple presentation will highlight the key points in differentiating, diagnosing these orders. Initial management principles are discussed as well.
It is a neoplasm of B-cell lineage; proliferation of the cells forms a monoclonal population of plasma cells and produces a single type of Ig/Ig fragment.
Plasma cell disorders is a difficult topic where most residents and students confuse with regarding to differentiating between various types of para-proteinemias or plasma cell dyscrasias. This simple presentation will highlight the key points in differentiating, diagnosing these orders. Initial management principles are discussed as well.
It is a neoplasm of B-cell lineage; proliferation of the cells forms a monoclonal population of plasma cells and produces a single type of Ig/Ig fragment.
UAEU - CMHS - Hematology-Oncology Course - MMH 302 - HONC 320. Education material for medical students - It cover basic principles of hematology and oncology, including CAR-T and gene editing. It can be used for study and review. It illustrates main principles of hematology and oncology.
Lab Diagnosis of Chronic lymphoproliferative disorders (CLPD);Flowcytometric...Dr Siddartha
Lab Diagnosis of Chronic lymphoproliferative disorders (CLPD);Flowcytometric Evaluation
Basavatarakam Indo-American Cancer Hospital and Research Institute
Cell blocks are an integral part of cytology preparations and ancillary testing.
In certain settings, such as molecular testing of lung cancer or by a commercial laboratory, they are the preferred cytology preparation.
To optimize them, care in specimen procurement, triage, and improvement in current processing techniques are necessary.
UAEU - CMHS - Hematology-Oncology Course - MMH 302 - HONC 320. Education material for medical students - It cover basic principles of hematology and oncology, including CAR-T and gene editing. It can be used for study and review. It illustrates main principles of hematology and oncology.
Lab Diagnosis of Chronic lymphoproliferative disorders (CLPD);Flowcytometric...Dr Siddartha
Lab Diagnosis of Chronic lymphoproliferative disorders (CLPD);Flowcytometric Evaluation
Basavatarakam Indo-American Cancer Hospital and Research Institute
Cell blocks are an integral part of cytology preparations and ancillary testing.
In certain settings, such as molecular testing of lung cancer or by a commercial laboratory, they are the preferred cytology preparation.
To optimize them, care in specimen procurement, triage, and improvement in current processing techniques are necessary.
multiple myeloma
Pathophysiology
Malignant proliferation of plasma cells in the bone marrow.
Causes bone marrow destruction via infiltration, and bone destruction via ↑RANKL activity (causing ↑osteoclast activity).
A single clone of plasma cells produce large amounts of identical immunoglobulin (a 'paraprotein' or 'monoclonal band'), as well as free κ or λ light chains (also a 'paraprotein', or 'Bence Jones protein' if in the urine).
Classified by Ig class, with prevalence reflecting prevalence in normal blood: IgG (⅔), IgA (⅓), remainder IgM or IgD.
Immunoglobulin classes other than that of the proliferating clone are relatively low ('immunoparesis').
Epidemiology
Lifetime risk: 1/140.
Incidence steadily increases with age. Rare <55.
Slightly commoner in men.
2x commoner in blacks vs. whites.
acute leukemia
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For Health benefits and medicine videos Subscribe youtube channel - https://www.youtube.com/playlist?list=PLKg-H-sMh9G01zEg4YpndngXODW2bq92w
Practical Hematology lectures as Manual CBC, shows count of blood cells white, Red and platelets.
Clinical Pathology
Hematology
Al-Azhar University
Clinical Examination
Iron is very important for Hemoglobin synthesis and avoidance for anemia so it is very important to understand to protect us from iron deficiency anemia & Iron overload
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
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micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...
Plasma cell Neoplasms 2021.pdf
1. Plasma Cell Neoplasms
DR. MOHAMD SHAHEEN
MBBCH, MSC, MD, IPCD, HHMD
LECTURER OF CLINICAL PATHOLOGY- AL-AZHAR UNIVERSITY
CHIEF INFECTION CONTROL TEAM EL-HUSSIEN HOSPITAL
8. Monoclonal gammopathy of
undetermined significance (MGUS)
Epidemiology: More common in males after 70 years.
Characteristics:
- BM Plasma cells < 10 % which interstitial, scattered or in small clusters.
- M-protein: < 3 g/dl with
- No Bence Jones protein.
- No evidence of other B cell LPDs.
- No Organ or tissue involvement:
- No end organ damage CRAB (hypercalcemia, renal impairment, anemia or bone lesions).
Malignant evolution: in 30% of cases after 25 years.
9. Types: MGUS are major 2 types
1- IgM MGUS = Lymphoid/lymphoplasmacytic MGUS.
2- Non-IgM MGUS = Plasma cell MGUS (80% of MGUS).
o They have different
- Genetic bases and
- Outcomes as malignant progression.
- Morphology, but this analysis is not always precise.
- 1% of plasma cell MGUS cases produce an IgM M protein.
- IgM MGUS is a lymphoplasmacytic lymphoma entity.
10. Non-IgM MGUS
Definition:
- Presence of an IgG, IgA, or (rarely) IgD M protein in the serum at a concentration < 3 g/dl
- BM plasma cells < 10%; and
- No end organ damage (CRAB) [hypercalcemia, Renal insufficiency, Anemia, and Bone lesions)
- No amyloidosis attributable to the plasma cell proliferative disorders
Epidemiology:
- Non IgM MGUS= 80% of MGUS.
- Male predominance 60%. [≈ ♂:♀= 2:1]
- Black : White populations = 2:1
Cell of origin: Post-germinal center plasma cells
11. Genetic profile:
- Conventional karyotyping are rarely found.
- FISH identifies numerical and/or structural abnormalities in most cases [as myeloma but
prevalence differ].
- Translocations involving the
1. Translocation of IGH locus 50% of cases,
2. t(11;14)(q13;q32) (IGH/CCND1) → 15―25%,
3. t(4;14)(p16.3;q32) (IGH/ NSD2 → 3―9%, and
4. t(14;16)(q32;q23) (IGH/M4F) → 1―5%
5. Hyperdiploidy 40%
6. Deletions of 13q → 35―40%
7. Activating KRAS mutations not detected in MGUS but present in 20% of myelomas
15. DD between MGUS [IgM & Non IgM]
Non-IgM MGUS
IgM-MGUS
Plasma cell neoplasm
Lympho-plasma cell lymphoma
Category
85% of MGUS
Male predominance
Black : White = 2:1
15% of MGUS
Male predominance
White > black
Incidence
Post germinal center plasma cell with IgHV gene but
without class switching
B cell with somatic hypermutation
Origin
PB:
- M protein unexpected in PE
- Imm.Fix: IgG 60%, IgA 15%, IgE 1%, IgD 1%
- Light chain 20%.
BM:
Hypercellular BM+ Plasma cells < 10%
BP: Serum IgM < 3 gm/dl
BM: lymphoplasmacytic cell < 10%
Lack of diagnostic feature of LPD, LPL, PCM.
Lab findings
2 populations:
Poly clonal normal Plasma cells: CD19+, CD56-.
Mono clonal aberrant Plasma cells: CD19-,56-/CD19-,56+
Clonal B cells: no specific IPT.
CD19+, 20+, CD5-, 10-, 103-
Plasma cells: 56-
I.P.T
- Serum IgM < 3 gm/dl
- Plasma cell < 10%
- No CRAB nor amyloidosis related to PCM
- Serum IgM < 3 gm/dl
- Plasma cell < 10%
- No CRAB, HSM, LN+, No Hyperviscosity
Diagnostic
Criteria
17. Plasma cell myeloma
Definition:
- Clonal proliferation of Ig secreting heavy chain class switched terminally differentiated
plasma B cells that produce a monoclonal protein in the serum or urine.
Epidemiology:
- Age: > 50 year not before 30 years. [adult disease]
- Sex: males predominance
18. Pathogenesis:
I- Genetic abnormalities (80-90%):
➢ Translocation of genes involved in ↑ cell survival and proliferation and
↓ apoptosis adjacent to Ig heavy or light chains.
- Ig heavy chain gene on chromosome (14) may rearranged adjacent to the following genes:
- 11q13 (CCND1).
- 6p21(CCND3).
- 4p16 (FGFR/MMSET). - 16q23(MAF). - 20q11(MAFB). - 8q24(RAS).
➢ Dysregulation of cyclin D gene.
➢ Micro RNA dysregulation.
➢ Aneuploidy:
- Monosomy 13. - (17q-). - (1q -).
- Hyperdiploidy.
➢ Methylation modification.
19. II- Cytokine interaction between plasma cells and their microenvironment:
▪ Chemotaxis between stromal cell derived factor (SCD-F) secreted by BM stromal
cells and CXCR4 on myeloma cells.
▪ Adhesion between intracellular adhesion molecule-1 (ICAM-1) and vascular cell
adhesion molecule1 (VCAM-1) on BM stromal cells and β1 integrin of myeloma
cells.
▪ BM homing of plasma cells due to expression of adhesion molecules on myeloma
cells as CD138, CD38, CD44 and CD106.
▪ Adhesion of myeloma cells to BM leads to:
- Cell cycle arrest at G1. - Decreased apoptosis.
- ↓ topoisomerase II activity e protection of myeloma cells from drugs.
- Secretion of cytokines as TNFα, IL6, IL21, ILGF-1, SCD-F and vascular endothelial
growth factor (VEGF) by both plasma cells and BM stromal cells.
20. Pathogenesis continue
III-Bone marrow infiltration by myeloma cells causes:
- Bone marrow failure.
- Cytokine production which suppress osteoblasts and stimulate osteoclasts causing
bone destruction that leads to bone pain, neurological manifestations and
hypercalcemia.
IV- Myeloma cells secret a monoclonal protein which causes:
- Hyperviscosity.
- Light chains precipitate in tissues causing amyloidosis.
- Light chain deposition in the renal tubules causing nephropathy.
21. V- Anemia may be due to:
- BM infiltration. - Bleeding.
- Anemia of chronic disorders. - Anemia of chronic renal failure.
- Chemotherapy.
VI- Recurrent infection may be due to:
- Suppression of the normal CD19(+ )B cells with suppression of the normal Igs.
- Reversed CD4+/CD8+ ratio. - Renal failure.
- Neutropenia. - Chemotherapy.
VII-Thrombosis may be due to:
- Hyperviscosity.
- Protein C deficiency and activated protein C resistance.
- Lupus anticoagulants. - Dysfibrinogenemia.
- Thialidomide treatment.
- Thrombosis from hyposplenism may be caused by amyloidosis.
22. VIII- Bleeding may occur due to:
- Hyperviscosity.
- Perivascular amyloidosis.
- Paraproteins inhibits fibrin aggregation.
- Antibodies against coagulation factors.
- Thrombocytopenia.
IX- Renal failure may be due to:
- Amyloidosis.
- Light chain deposition (Tamm-Horsfall protein).
- Recurrent infection.
- Hypercalcemia.
23. X-Pain may be caused by:
- Vertebral compression Fracture.
- Pathological fracture.
- Compression of spinal.
- Amyloid deposition.
24. Clinical picture
Bone: Back pain, collapse of the vertebrae, osteoporosis and pathological fracture.
Renal disease.
Pain.
Infection.
Hyperviscosity: [Thrombosis tendency] More in IgA due to tendency of IgA to form
polymers.
Amyloidosis: Macroglossia, carpal tunnel syndrome, gastrointestinal disturbance,
neuropathy and liver infiltration.
BM failure:
- Symptoms of anemia: pallor, fatigue, weakness, …..
- Symptoms of neutropenia: recurrent infection.
- Symptoms of thrombocytopenia: bleeding tendency.
CRAB
- Calcium elevation
- Renal Impairment (↑ urea& creatinine)
- Anemia
- Bone (pain, fractures)
25. Laboratory diagnosis
o CBC:
- May be completely normal:
- Anemia.
- Thrombocytopenia & may be Thrombocytosis due to the hyposplenism caused
by amyloid deposition.
o Blood film:
- Blue background due to the high protein.
- Increased reuleaux.
o ESR: Very high>100mm/hr. [Inverted A/G ratio+ hyperviscosity].
o Total protein: High and low serum albumin.
o 24-Hours urine: Bence jones protein.
26. o Serum and urine protein electrophoresis:
Monoclonal M-band.
o Immunofixation: detects the type of the Ig which is:
- IgG in 50% of cases.
- IgA in 20% of cases.
- Light chain in 20% of cases.
- Ig E.
- IgD and IgM biclonal in less than 10% of cases.
28. Peripheral blood
Rouleaux formation is the most striking feature and is related to quantity
and type of M protein.
A leukoerythroblastic reaction.
Plasma cells are found in 15% of cases, usually in small numbers.
Marked plasmacytosis indicates plasma cell leukemia.
Kidney Bence Jones protein accumulates as aggregates of eosinophilic
material in the lumina of the renal tubules. Renal tubular reabsorption of
Bence Jones protein is largely responsible for renal damage in PCM.
29. - Hypercellular BM.
- Myeloma plasma cells of different stages of maturation
- Dry tap may be present
- Fragile bone
Bone Marrow
30. Bone marrow aspiration
Plasma cells varies from little increased to > 90%.
Myeloma plasma cells vary from mature forms indistinguishable from normal cells to immature, plasmablastic, and
pleomorphic cells.
Mature plasma cells: usually oval, with a round eccentric nucleus and so-called spoke-wheel or clock-face chromatin
without nucleoli. abundant basophilic cytoplasm and a perinuclear hof. The small-cell variant shows a
lymphoplasmacytic appearance, with a narrow rim of basophilic cytoplasm and the occasional perinuclear hof.
Immature forms have more-dispersed nuclear chromatin, a higher N:C ratio, and (often) prominent nucleoli. In almost
10% of cases, there is plasmablastic morphology. Multinucleated, multilobed, pleomorphic plasma cells are prominent
in some cases. Nuclear immaturity and pleomorphism rarely occur in reactive plasma cells, so they are indicators of
neoplastic plasma cells. The cytoplasm of myeloma cells has abundant endoplasmic reticulum, contain condensed or
crystallized cytoplasmic Ig producing a variety of morphological findings, including multiple pale bluish-white, grape-
like accumulations (Mott cells and morula cells); cherryred refractive round bodies (Russell bodies); vermilion-staining
glycogen-rich IgA (flame cells); overstuffed fibrils (pseudoGaucher cells and thesaurocytes); and crystalline rods. These
changes are not pathognomonic of PCM; they can also be found in reactive plasma cells.
In 5% of cases of PCM, there are < 10% plasma cells in BM aspirate smears. This may be due to a suboptimal BM
aspirate or the frequent focal distribution of PCM in BM.
BM biopsy: larger numbers of plasma cells and focal clusters. Biopsies directed at radiographical lesions may be
necessary to establish the diagnosis in some patients.
32. - Pattern of infiltration: Interstitial clusters or sheets.
- Plasma cells or masses of plasma cells ≥ 30% of BM volume is diagnostic.
- Prominent osteoclastic activity.
- Myelodysaplasia may develop after therapy.
o Immunophenotyping [FCM + IHC]:
- CD19(-), and Surface membrane Ig(-) but it is assigned to B cell lineage because
it is CD79a(+) and cytoplasmic Ig(+).
- Light chain restriction.
- CD38(+), CD138(+), CD56(+) and CD10(+).
- Cyclin D1 is (+) in t(11;14)(q13;32).
Bone marrow biopsy
33. o Cytogenetic: [Remember 13-14--16-17]
1- Ig heavy chain gene rearrangement of on chromosome (14) with either of the following:
- 11q13 (CCND1).
- 6p21(CCND3).
- 4p16 (FGFR/MMSET).
- 16q23(MAF).
- 20q11(MAFB).
- 8q24(RAS).
2- Dysregulation of cyclin D gene.
3- Micro RNA dysregulation.
4- Aneuploidy:
- Monosomy 13. - (17q-). - (1q -).
- Hyperdiploidy.
Cytogenetic
34. Lab investigation cont.
o Plasma cell labeling index by a tritiated thymidine or bromoeoxyuridine techniques.
- It is incorporated in S-phase.
- If less than 0.5, it means a short survival.
o Amyloid deposition by congo red stain.
o Serum β2 microglobulin: High.
o Biochemical tests:
- Serum uric acid: High.
- Serum Calcium: High.
- Kidney function: High urea & creatinine.
o Coagulation tests: Prolonged.
o Platelet function: impaired.
35. - Skeletal involvement in the form of lytic lesion and/or severe
osteoporosis with or without compression fracture.
X-Ray shows
- A frontal view of the shoulder in a patient with multiple
myeloma.
- The solid arrow indicates a chronic pathologic humeral
fracture at the surgical neck across a large lytic lesion
with a "moth-eaten" appearance down the humeral
shaft.
- The clavicle and ribs are also affected.
- The open arrows reveal a missing fifth rib, which was
destroyed by a plasmacytoma (plasma cell tumor) (open
arrows).
oRadiological examination
36. Differential diagnosis
Increased plasma cells in the BM:
1. Benign BM plasmacytosis: Infection. Autoimmune diseases.
2. B-cell NHL with plasmacytic differentiation.
3. Plasma cell neoplasms:
o MGUS.
o Plasma cell myeloma and variants: Asymptomatic (Smouldering), Non-secretory myeloma &
Plasma cell leukemia.
o Plasmacytoma:
- Solitary plasmacytoma of bone.
- Extraosseous (extramedullary) plasmacytoma.
o Ig deposition diseases:
- Primary amyloidosis (Ig light chain amyloidosis).
- Systemic light and heavy chain deposition diseases.
o Osteosclerotic myeloma (POEMS syndrome: polyneuropathy – organomegaly- endocrinopathy –
M protein – Skin).
37. II- Differential diagnosis of hyperviscosity:
1- Waldenstrom’s macroglobulinemia:
- The monoclonal protein is IgM and
- The malignant cells may be lymphocytes, lymphoplasmacytoid cells or plasma
cells which are
CD19(+), surface Ig(+), CD38(-), CD138(-) and CD56(-).
2-Plasma cell neoplasm.
3- Polycythemia: ↑ RBCs count, ↑ Hb conc. & ↑ hematocrit.
4- Thrombocytosis: ↑ platelet count.
5- Hyperleucocytosois
38. III- Differential diagnosis of monoclonal proteins:
Multiple
Myeloma
Smoldering
myeloma
MGUS
In serum & urine
≥ 3 g/dl
> 3 g/dl
M Protein
> 10
> 10
> 10
BM Plasma cell
yes
No
No
Tissue/Organ impaired
Myeloma cell
Normal plasma
cell
CD19-ve
CD19+ve
CD19
CD56+ve
CD56-ve
CD56
CD38 dimmer
CD38+
CD38
brighter
CD138+
CD138
44. Non-secretory myeloma
Incidence: ≈ 1% of PCMs.
Characters:
- No M protein by serum &urine immunofixation electrophoresis.
- Cytoplasmic M protein is present in the neoplastic plasma cells in about 85% of
cases by IHC, consistent with production but impaired secretion of Ig.
- No cytoplasmic Ig: 15% of non-secretory myelomas, synthesis is detect
- serum free light chains ± an abnormal free light chain ratio: in 2/3 of cases & is
considered to be non-secretory by immunofixation electrophoresis, suggesting that
many such cases are at least minimally secretory or oligosecretory.
45. Pathogenesis:
Acquired mutations of the IG light chain variable genes and alteration in the light
chain constant region.
Clinical features: as other PCMs, except for a lower incidence of renal
insufficiency and hypercalcaemia and less depression of normal Ig.
Immunophenotype, genetics, and prognosis: as other PCMs.
Survival appears to be better for patients with a normal baseline serum free light
chain ratio than those with an abnormal ratio.
Non-secretory myeloma must be distinguished from the rare IgD and IgE
myelomas, which have low serum M protein and may not be routinely screened
for by immunofixation electrophoresis.
46. Plasma cell leukemia
▪ Definition: Clonal proliferation of plasma cells infiltrating the peripheral blood with tissue
affection.
▪ Origin:
1- De novo presentation in leukemic phase.
2- Secondary to multiple myeloma.
▪ Pathogenesis: Genetic abnormalities which affect the regulation of the cell cycle, and
plasma cell proliferation, survival, apoptosis and adhesion to BM.
▪ Clinical picture: Hypercalcemia, renal impairment and anemia with less bone lesions.
47. Laboratory diagnosis:
1- CBC:
- Anemia and thrombocytosis.
- Leukocytosis up to 90-150 X 109 /L.
2- Blood film:
- Plasma cells are ≥ 20% or > 2X 109/L.
- Blue background.
- Increased RBCs reuleaux.
3- ESR: Very high.
4- Total protein =↑and serum albumin = ↓.
5- Serum and urine protein electrophoresis: Monoclonal band.
6- Immunofixation: Light chain only, IgD or IgE.
48. 7- Bone marrow:
- Hypercellular BM with plasma cells less than in MM which may resemble plasma cells or
lymphoplasmacytoid lymphocytes.
8- Immunophenotyping [FCM & IHC]:
- CD19(-), and surface Ig(-) but it is assigned to B cell lineage because it is CD79a(+) and
cytoplasmic Ig(+).
- Light chain restriction.
- CD38(+), CD138(+), CD56(-) and CD10(+).
9- Cytogenetics: t(11;14).
10-Biochemical tests: High serum calcium and creatinine.
Prognosis: very aggressive and resistant to therapy.
50. Plasmacytoma
Definition:
- Solitary plasmacytomas are single localized tumours consisting of monoclonal plasma
cells with no clinical features of plasma cell myeloma (PCM) and no physical or
radiographical evidence of additional plasma cell tumours.
Two types of plasmacytoma:
- Solitary plasmacytoma of bone and
- Extraosseous (extramedullary) plasmacytoma.
51. Solitary plasmacytoma of bone
(SPB).
- Definition
- SPB is a localized tumour consisting of monoclonal plasma cells with no clinical
features of PCM. Radiographical studies, including MRI and CT, show no other bone
lesions.
- 30% of patients with a SPB defined only by radiographical skeletal survey.
- Epidemiology
- 1 % of plasma cell neoplasms.
- More common in men (65% of cases),
- Median age is 55 years.
53. Extraosseous plasmacytoma
Definition
- Localized plasma cell neoplasms that arise in tissues other than bone.
- Lymphomas with prominent plasmacytic differentiation, especially
extranodal MZL of mucosa-associated lymphoid tissue (MALT lymphoma),
must be excluded.
Epidemiology
- 1% of plasma cell neoplasms
- 2/3 of patients are male,
- Median age = 55 years
54. Differential diagnosis of neoplasms with plasmacytic (PC) or
plasmablastic (PB) differentiation in extraosseous locations
57. Monoclonal Ig deposition diseases
Definition
- They are closely related disorders characterized by visceral and soft tissue deposition of
aberrant Ig, resulting in loss organ function.
- These disorder is typically a plasma cell neoplasm, or rarely a lymphoplasmacytic neoplasm;
however, the Ig molecule usually accumulates in tissue before the development of a large
tumour burden.
- Therefore, patients typically do not have overt myeloma or lymphoma at the time of the
diagnosis.
2 major types:
- Primary amyloidosis and
- Light chain and heavy chain deposition diseases.
- These disorders have chemically different manifestations of similar pathological processes,
resulting in clinically similar conditions.
58. Primary amyloidosis
Definition
-Primary amyloidosis is caused by a plasma cell or (rarely) a lymphoplasmacytic neoplasm in
which the monoclonal plasma cells secrete intact or fragments of abnormal Ig light chains that
deposit in various tissues and form a beta-pleated sheet structure (amyloid light chain).
Epidemiology
- Incidence: 1 /100 000 population,
- Age: Median age is 64 years & 95% > 40 years;
- Sex: 70% are male = male : female= 2:1.
- 20% of patients with primary amyloidosis have PCM.
59. Macroscopy
- Amyloid has a porcelain-like or waxy appearance.
- Site of Amyloid deposits: 60% in BM & also present in other tissues and organs.
Microscopy
- BM biopsy: vary from revealing no pathological findings to showing extensive replacement with amyloid, overt PCM, or
(rarely) involvement with lymphoplasmacytic lymphoma (LPL).
- Mild ↑ in plasma cells (commonest finding), which may appear normal or show any of the changes found in myeloma.
On H&E-stained sections,
- Amyloid is a pink, amorphous, waxy-looking substance with a characteristic cracking artifact.
- Macrophages and foreign-body giant cells may be found around deposits.
- Amyloid rarely massively replace organ parenchyma .
- Plasma cells may be increased in the adjacent tissues.
Congo red stains amyloid:
- Pink to red by standard light microscopy, and
- Apple-green birefringence under polarized light .
Congo red fluorescence microscopy:more sensitive method for amyloid detection.
Tissue biopsy
60.
61. Light chain and heavy chain
deposition diseases (LCDD & HCDD)
Definition
- Monoclonal LCDD and HCDDs are plasma cell or (rarely) lymphoplasmacytic
neoplasms that secrete an abnormal light or (less often) heavy chain or both, which
deposit in tissues, causing organ dysfunction, but do not form amyloid beta-pleated
sheets, bind Congo red stain, or contain an amyloid P component.
These disorders comprise:
- Light chain deposition disease (LCDD),
- Heavy chain deposition disease (HCDD), and
- Light and heavy chain deposition disease (LHCDD)
63. POEMS syndrome
Definition: It is a para-neoplastic syndrome associated with a plasma cell
neoplasm, usually characterized by
- Fibrosis and
- Osteosclerotic changes in bone trabeculae, and
- Often with lymph node changes resembling the plasma cell variant of
Castleman disease.
Abrreviation:
- Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy,
and Skin changes
65. Immunophenotyping:
- The neoplastic plasma cells are of IgG or IgA type, and are lambda-restricted in almost all cases.
Genetic profile: as PCM
Prognosis:
- Poor prognostic factors:
- Extravascular fluid overload,
- Fingernail clubbing,
- Respiratory symptoms, and
- Pulmonary hypertension
66. TEMPI syndrome
Definition: It is a para-neoplastic syndrome associated with a plasma cell neoplasm
It is a rare and recently described disease [11 cases only].
Abbreviation:
- Telangiectasias, Elevated Erythropoietin and Erythrocytosis, Monoclonal gammopathy,
Perinephric fluid collection, and Intrapulmonary shunting.
-
It similar to POEMS syndrome in its manifestations that appear to result from the monoclonal
plasma cell proliferation and associated M protein. However, the clinical and laboratory
findings are mostly distinct from those of POEMS syndrome.
TEMPI syndrome is a rare and only recently described disease.
67. Microscopy:
- No specific finding in Pb or BM, but
- BM: Erythrocytosis and a hypercellular BM due to erythroid hyperplasia are recurrent findings.
- Mild erythroid and megakaryocytic atypia has been described in one patient, and reactive
lymphoid aggregates were present in another.
- BM clonal plasma cells % in the range of MGUS ( 10% plasma cells)
Immunophenotyping
- Plasma cell is most commonly IgG kappa, but both IgG and IgA lambda have also been reported.
- There are no detailed descriptions of IPT of monoclonal plasma cells.
69. Waldenstrom Macroglobulinemia
Lymphoplasmacytic lymphoma
WM: (mainly secretory cells) LPL(mainly proliferating cells)
Definition: Post-germinal center lymphoid neoplasm with accumulation of a clonal population
of lymphocytes, lymphoplastic cells and plasma which secrete a monoclonal IgM.
Etiology:
1- Familial.
2- HCV infection.
3- Radiation.
4- Genetic abnormalities.
- Point mutation of MYD88 gene in 90-100% of cases leading to high expression of nuclear factor
kβ (NF-kβ).
- Loss of all or part of chromosomes (17), (18), (19), (20), (21), (22), (X) or (Y).
- Gain in chromosomes (3), (4) or (12). - (6q-) in 50% of cases.
- No Ig heavy chain rearrangement which differentiates it from IgM plasma cell myeloma.
Epidemiology: More in males after 60 years
70. Clinical picture:
Splenomegaly and hepatomegaly in < 15% of cases.
Symptoms of Hyperviscosity: Headache, blurring of vision and loss of
concentration.
Hemorrhage: may be due to:
- Dysfibrinogenemia.
- Thrombocytopenia.
Symptoms of Neuropathy.
Symptoms of Anemia; may be due to:
- ↓ erythropoietin level due to the hyperviscosity.
- ↓RBCs survival due to the anti I or I Antibodies.
- Moderate plasma volume expansion due to splenomegaly.
- Blood loss from the GIT.
- BM infiltration.
71. Laboratory investigations
1- CBC:
- Anemia with inaccurate ↑ Hb estimation due to interaction between monoclonal proteins and diluent of
automated analyzer.
- Severe thrombocytopenia.
2- Blood film: Reuleaux and RBCs aggregation.
3- Coagulation: Prolonged PT, aPTT and TT.
4- Platelet functions: ↓platelet aggregation.
5- Bone marrow biopsy:
a- Hypercellular BM.
b- Infiltration by lymphoplasmacytic cells with evidence of plasmacytoid and plasma cell maturation.
c- Pattern of infiltration: Diffuse, interstitial, nodular or intertrabecular.
d- Cytochemistry: PAS (+) intranuclear inclusions.
e- Increased mast cells in association with lymphoid aggregates
72. 6- Immunophenotyping:
- CD19(+), surface IgM(+), CD20(+), CD22(+) and CD79a(+).
- Up to 20% of cases are CD5(+), CD10(+) and CD 23(+).
7- Protein electrophoresis: Monoclonal band.
8- Bence Jones protein:
- Positive in 70% of cases but ≤ 1g/24 hours urine.
9- Immunofixation: IgM monoclonal protein.
10- Auto antibodies as: Anti-erythropoietin antibodies, anti-amylein antibodies, anti-vWF Abs.
11- Positive tests for cryoglobulins and cold agglutinins.
12- Increased serum viscosity.
13- Genetic studies:
- Point mutation of MYD88 gene in 90-100% of cases.
- Loss of all or part of chromosomes (17), (18), (19), (20), (21), (22), (X) or (Y).
- Gain in chromosomes (3), (4) or (12).
- (6q-) in 50%of cases.
- No Ig heavy chain rearrangement.