Practical Exam of clinical pathology, [Bone marrow slides, blood film, R.C slides, Hb electrophoresis, HPLC and others

1. Dr. Mohamed Shaheen
MBBCh, MSc, MD, IPCD, HHMD
Lecturer of Clinical Pathology- Al-Azhar University
Chief of Infection Control team El-Hussien hospital

2. RBCs
Spot diagnosis
Cases (L/S)
Electrophoresis
HPLC
ESR
Reticulocyte count
Coombs test
Osmotic fragility
WBCs
Spot diagnosis
Cases (L/S)
CBC Histogram
CBC Manual
FCM
Hemostasis
Coagulation tests
•Mixing study
•PT & PTT
PLT aggregation
Blood bank
ABO discrepancy
AB screen
Genetics
MCQ
7/7/2023
Dr.Mohamed Shaheen
Practical Exam items

3. Exam schedule approximation
Degree 100
Tools
Sample
Test
10
Microscope + slide+ cover + Oil
Slide
Spot diagnosis slides
50
Microscope + slide+ cover + Oil
Slides
Long Cases
5
Microscope + slide+ cover + Oil
Slide
Pb differential count
5
Microscope + slide+ cover + Oil
Slide
Retix count
5/6
Hemocytometer + Cover slide
3 Diluents + Petri dish
Pipets + tips
CBC EDTA
Manual CBC
5/4
ESR tube + rack
CBC EDTA
ESR
5
Glass Tubes + Reagents
Stopwatch + Water bath
PT citrated
Coagulation test
5
Paper+ pen
Reports
FCM
10
Paper+ pen
Reports
Hb electro

4. Examples of Exam schedule
Short/Long cases
Spot
Test
AML (M1-M7)
AML (M1-M7)
AML
ALL (L1,2,3)
ALL (L1,2,3)
ALL
CML
CML
MPN
CLL, PLL, HCL
CLL, PLL, HCL
LPN
MDS
--
MDS
MM
MM
Plasma C.N
Gushier, Niemann pick
Chediak Higashi
Gushier, Niemann pick
Chediak Higashi
Macrophage
Lysozyme
ITP
--
PLT
Megaloblastic anemia
- Hemolytic anemia
- Megaloblastic anemia
- Sphero, elliptocytosis
- Thalassemia (Normoblasts)
- IDA, SCA, HbH
- Malaria, Filarial, Leishmania
RBCs
Parasites
BM infiltration
Neuroblastoma, Histocytosis
Others

5. Exam simulation

6. Long and Short cases
 WBCs: Leukemia:
- Acute: AML (1-7), ALL (L1,2,3).
- Chronic: MPN: CML, & LPD: CLL, PLL, HCL.[DD; FL, MCL, reactive lymphocytosis, MBL]
- PCN: MM, [DD; WM, MGUS].
- Reactive lymphocytosis (IMN).
- Monocytes & lysozyme disorders: Gaucher D, Neumann Pick,
 RBCs: Anemia:
- Megaloblastic anemia, Hemolytic anemia (thalassemia), IDA, Spherocytosis,
Elliptocytosis, Sickle cell anemia, HbH disease
- Parasites: Malaria, Filaria, Leishmania.
 Platelets: ITP.
 Neuroblastoma.

7. Long cases comments
 Pb comment:
- WBCs,
- RBCs &
- PLTs.
 BM Comment:
- Myeloid:
- Erythroid: M:E ratio:
- Megakaryocyte:
 Conclusion:
 Recommendations:
- IPT/FCM
- Cytogenetics
 DD;

8. Exam items
 ABO discrepancy, Ab screen
 BM-Long Cases
 Coagulation case (Mixing study) & Tests (PT)
 Differential count-PB
 Discussion (Oral )
 Electrophoresis [Hb / Protein]
 FCM
 Genetics
 HPLC
 MCQ
 RBCs tests: Reticulocyte count, ESR, Coombs test, Osmotic fragility

9. Degree 100
Tools
Sample
Test
10
Microscope + slide+ cover + Oil
Slide
Spot diagnosis slides
50
Microscope + slide+ cover + Oil
Slides
Long Cases
5/10
Microscope + slide+ cover + Oil
Slide
Pb differential count
5
Microscope + slide+ cover + Oil
Slide
Retix count
6
Hemocytometer + Cover slide
3 Diluents + Petri dish + Pipets + tips
CBC EDTA
CBC manual
4
ESR tube + rack
CBC EDTA
ESR
5
Glass Tubes + Reagents
Stopwatch + Water bath
Citrated blood
Coagulation test
5/10
Paper+ pen
Reports
FCM
10
Paper+ pen
Reports
Hb electro
5/10
O.F tubes + Rack
Tubes
Osmotic fragility
5/10
Paper+ pen
CBC strip
Histogram
7/7/2023
Dr.Mohamed Shaheen
Diploma Exam schedule
‫دلور‬ ‫دور‬ ‫ومن‬ ‫ملاكن‬ ‫ماكن‬ ‫من‬ ‫خيتلف‬ ‫العميل‬ ‫الامتحان‬ ‫ليوم‬ ‫تقديري‬ ‫تصور‬ ‫مثال‬

10. Degree 100
Tools
Sample
Test
10
Microscope + slide+ cover + Oil
Slide
Spot diagnosis slides
50
Microscope + slide+ cover + Oil
Slides
Long Cases
5/10
Microscope + slide+ cover + Oil
Slide
Pb differential count
5
Microscope + slide+ cover + Oil
Slide
Retix count
6
Hemocytometer + Cover slide
3 Diluents + Petri dish + Pipets + tips
CBC EDTA
CBC manual
4
ESR tube + rack
CBC EDTA
ESR
5
Glass Tubes + Reagents
Stopwatch + Water bath
Citrated blood
Coagulation test
PT
5/10
Paper+ pen
Reports
FCM
10
Paper+ pen
Reports
Hb electro
5/10
O.F tubes + Rack
Tubes
Osmotic fragility
7/7/2023
Dr.Mohamed Shaheen
Master Exam schedule
‫دلور‬ ‫دور‬ ‫ومن‬ ‫ملاكن‬ ‫ماكن‬ ‫من‬ ‫خيتلف‬ ‫العميل‬ ‫الامتحان‬ ‫ليوم‬ ‫تقديري‬ ‫تصور‬ ‫مثال‬

11. Doctorate Exam schedule
‫تصور‬
‫دلور‬ ‫دور‬ ‫ومن‬ ‫ملاكن‬ ‫ماكن‬ ‫من‬ ‫خيتلف‬ ‫العميل‬ ‫الامتحان‬ ‫ليوم‬ ‫تقديري‬
Degree 100
Tools
Sample
Test
10
Microscope + slide+ cover + Oil
Slide/10
Spot diagnosis slides
5
Paper+ pen
Case
ABO discrepancy
50
Microscope + slide+ cover + Oil
Slides/5
BM-Long Cases
5
Paper+ pen
Reports
Coagulation case
- PLT aggregation
- Mixing study
10
Paper+ pen
Reports
Electrophoresis Hb/Ptn
10
Paper+ pen
Reports
FCM
10
Paper+ pen
Reports
Genetics
10
Paper+ pen
Reports
HPLC
7/7/2023
Dr.Mohamed Shaheen