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SAURABH ANIL BANE
S.I.E.S. COLLEGE
M.Sc.II ROLL NO.11
•Phytoremediation is the use of plants to clean up a
contamination from soils, sediments, and water.
•Takes the advantage of the unique capabilities of plant root
systems -translocation, bioaccumulation, and contaminant
degradation
► Phyton = Plant (in Greek) & Remediare = To remedy (in Latin).
•Traditional treatments are expensive and cost prohibitive.
•Some treatments that are available include:
TRADITIONAL TREATMENTS FOR SOIL CONTAMINATION
•Phytoremediation is on average tenfold cheaper than
engineering-based remediation methods
•It is usually carried out in situ
phyto-remediation can be broadly categorised as::
To Treat Organic
Contaminants :
• Phytodegradation
• Phytostimulation
• Phytovolatilisation.
To Treat Metal
Contaminants :
• Phytoextraction
• Rhizofiltration
• Phytostabilisation
PHYTOREMEDIATION
The Use of Phytoremediation to Treat Organic Contaminants
► Phytodegradation
•Breakdown of contaminants taken up by plants through
metabolic processes or through the effect of compounds (such
as enzymes) produced by the plants.
•Enzymes used – Nitroreductases,
Dehalogenases and laccases
eg.Populus species and
Myriophyllium spicatum .
► Phytostimulation
• Is the breakdown of contaminants in the rhizosphere through
microbial activity that is enhanced by the presence of plant
roots.
•Natural substances released by the plant roots – sugars,
alcohols, and acids – contain organic carbon
► Phytovolatilisation.
•Is the uptake and transpiration of a contaminant by a plant,
with release of the modified form of the contaminant from
the plant to the atmosphere.
•Poplar trees at one particular study site have been shown to
volatilise 90% of the TCE they take up.
The Use of Phytoremediation to Treat Metal Contaminants
► Phytoextraction
•Is the process of planting a crop of a species that is
known to accumulate contaminants in the shoots and
leaves, and then harvesting the crop and removing the
contaminant from the site.
•The harvested plant tissue is easily and safely processed
by drying, ashing or composting.
•Synthetic chelates has been shown to stimulate the release
of metals into soil solution and enhance the potential for
uptake into roots.
Schematic representation of the processes of natural (A) and assisted (B) phytoextraction.
► Rhizofiltration-
•Rhizofiltration (‘rhizo’ means ‘root’) is the adsorption or
precipitation onto plant roots (or absorption into the roots) of
contaminants that are in solution surrounding the root zone.
For example, sunflowers were successfully used to remove radioactive contaminants from pond
water in a test at Chernobyl, Ukraine.
► Phytostabilisation
•Is the use of plant species to immobilize contaminants in the
soil and groundwater through absorption and accumulation by
roots, adsorption onto roots, or precipitation within the root zone
of plants .
•This process prevents migration to the groundwater or air, and
reduces bioavailability for entry into the food chain .
•Metal-tolerant
species can be used to
restore vegetation to
the sites
•For enhancing natural phytoremediation capabilities, or for
introducing new capabilities into plants.
•Eg.-Bioengineering of plants (Arabidopsis and tobacco) capable
of volatilizing mercury from soil contaminated with methyl-
mercury
THE ROLE OF GENETICS
ADVANTAGES
• Lower cost technology.
• Requires less equipment and labor than other methods
• Cause little disruption to the site or surrounding community.
• It also reduces soil erosion and dust emissions.
• Make a site more attractive, and improve surrounding air
quality.
LIMITATIONS
• Slow and time consuming.
• Depends on tolerance of plants to the pollutants
• Requires large surface area of land.
• Climatic or seasonal variation can effect the growth
of plants and hence increase the time for remediation
• Environmental Science & Technology. 1998. Phytoremediation;
forecasting. Environmental Science & Technology. Vol. 32, issue
17, p.399A.
• McGrath, S.P. 1998. Phytoextraction for soil remediation. p.
261-287. In R. Brooks (ed.) Plants that hyperaccumulate heavy
metals their role in phytoremediation, microbiology,
archaeology, mineral exploration and phytomining.
• Phytoextraction of Toxic Metals: A Review of Biological
Mechanisms, Mitch M. Lasat.
•http://www.aslaoregon.org/updates/articles/phytoremediation
-in-landscape-architecture
REFERENCES
THANK YOU
YNWA►

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Phytoremediation 21 2-2015

  • 1. SAURABH ANIL BANE S.I.E.S. COLLEGE M.Sc.II ROLL NO.11
  • 2. •Phytoremediation is the use of plants to clean up a contamination from soils, sediments, and water. •Takes the advantage of the unique capabilities of plant root systems -translocation, bioaccumulation, and contaminant degradation ► Phyton = Plant (in Greek) & Remediare = To remedy (in Latin).
  • 3. •Traditional treatments are expensive and cost prohibitive. •Some treatments that are available include: TRADITIONAL TREATMENTS FOR SOIL CONTAMINATION
  • 4. •Phytoremediation is on average tenfold cheaper than engineering-based remediation methods •It is usually carried out in situ phyto-remediation can be broadly categorised as:: To Treat Organic Contaminants : • Phytodegradation • Phytostimulation • Phytovolatilisation. To Treat Metal Contaminants : • Phytoextraction • Rhizofiltration • Phytostabilisation PHYTOREMEDIATION
  • 5. The Use of Phytoremediation to Treat Organic Contaminants ► Phytodegradation •Breakdown of contaminants taken up by plants through metabolic processes or through the effect of compounds (such as enzymes) produced by the plants. •Enzymes used – Nitroreductases, Dehalogenases and laccases eg.Populus species and Myriophyllium spicatum .
  • 6. ► Phytostimulation • Is the breakdown of contaminants in the rhizosphere through microbial activity that is enhanced by the presence of plant roots. •Natural substances released by the plant roots – sugars, alcohols, and acids – contain organic carbon ► Phytovolatilisation. •Is the uptake and transpiration of a contaminant by a plant, with release of the modified form of the contaminant from the plant to the atmosphere. •Poplar trees at one particular study site have been shown to volatilise 90% of the TCE they take up.
  • 7. The Use of Phytoremediation to Treat Metal Contaminants ► Phytoextraction •Is the process of planting a crop of a species that is known to accumulate contaminants in the shoots and leaves, and then harvesting the crop and removing the contaminant from the site. •The harvested plant tissue is easily and safely processed by drying, ashing or composting. •Synthetic chelates has been shown to stimulate the release of metals into soil solution and enhance the potential for uptake into roots.
  • 8. Schematic representation of the processes of natural (A) and assisted (B) phytoextraction.
  • 9. ► Rhizofiltration- •Rhizofiltration (‘rhizo’ means ‘root’) is the adsorption or precipitation onto plant roots (or absorption into the roots) of contaminants that are in solution surrounding the root zone. For example, sunflowers were successfully used to remove radioactive contaminants from pond water in a test at Chernobyl, Ukraine.
  • 10.
  • 11. ► Phytostabilisation •Is the use of plant species to immobilize contaminants in the soil and groundwater through absorption and accumulation by roots, adsorption onto roots, or precipitation within the root zone of plants . •This process prevents migration to the groundwater or air, and reduces bioavailability for entry into the food chain . •Metal-tolerant species can be used to restore vegetation to the sites
  • 12. •For enhancing natural phytoremediation capabilities, or for introducing new capabilities into plants. •Eg.-Bioengineering of plants (Arabidopsis and tobacco) capable of volatilizing mercury from soil contaminated with methyl- mercury THE ROLE OF GENETICS
  • 13. ADVANTAGES • Lower cost technology. • Requires less equipment and labor than other methods • Cause little disruption to the site or surrounding community. • It also reduces soil erosion and dust emissions. • Make a site more attractive, and improve surrounding air quality.
  • 14. LIMITATIONS • Slow and time consuming. • Depends on tolerance of plants to the pollutants • Requires large surface area of land. • Climatic or seasonal variation can effect the growth of plants and hence increase the time for remediation
  • 15. • Environmental Science & Technology. 1998. Phytoremediation; forecasting. Environmental Science & Technology. Vol. 32, issue 17, p.399A. • McGrath, S.P. 1998. Phytoextraction for soil remediation. p. 261-287. In R. Brooks (ed.) Plants that hyperaccumulate heavy metals their role in phytoremediation, microbiology, archaeology, mineral exploration and phytomining. • Phytoextraction of Toxic Metals: A Review of Biological Mechanisms, Mitch M. Lasat. •http://www.aslaoregon.org/updates/articles/phytoremediation -in-landscape-architecture REFERENCES

Editor's Notes

  1. Phytoremediation is on average tenfold cheaper than engineering-based remediation methods such as soil excavation, soil washing or burning, or pump-and-treat systems. It is usually carried out in situ contributes to its cost-effectiveness and may reduce exposure of the polluted substrate to humans, wildlife, and the environment.
  2. Phytodegradation Phytodegradation, also called phyto-transformation, is the breakdown of contaminants taken up by plants through metabolic processes within the plant, or the breakdown of contaminants surrounding the plant through the effect of compounds (such as enzymes) produced by the plants. Complex organic pollutants are degraded into simpler molecules and are incorporated into the plant tissues to help the plant grow faster . Plant enzymes used for the degradation include nitroreductases (degradation of nitroaromatic compounds), dehalogenases (degradation of chlorinated solvents and pesticides) and laccases (degradation of anilines). Populus species and  Myriophyllium spicatum are examples of plants that have these enzymatic systems 
  3. Phyto-stimulation also called Rhizodegradation or plant-assisted bioremediation /degradation, is the breakdown of contaminants in the rhizosphere (soil surrounding the roots of plants) through microbial activity that is enhanced by the presence of plant roots and is a much slower process than phytodegradation. ► Phytostimulation Certain micro-organisms can digest organic substances such as fuels or solvents that are hazardous to humans and break them down into harmless products in a process called biodegradation. Natural substances released by the plant roots – sugars, alcohols, and acids – contain organic carbon that provides food for soil microorganisms and the additional nutrients enhance their activity. ► Phytovolatilisation. Phytovolatilisation is the uptake and transpiration of a contaminant by a plant, with release of the contaminant or a modified form of the contaminant from the plant to the atmosphere. Phytovolatilisation occurs as growing trees and other plants take up water and the organic contaminants. Some of these contaminants can pass through the plants to the leaves and evaporate, or volatilise, into the atmosphere. Poplar trees at one particular study site have been shown to volatilise 90% of the TCE they take up.
  4. Phytoextraction, or phytomining, is the process of planting a crop of a species that is known to accumulate contaminants in the shoots and leaves of the plants, and then harvesting the crop and removing the contaminant from the site. The harvested plant tissue, rich in accumulated contaminant, is easily and safely processed by drying, ashing or composting. The potential for phytoextraction of several major metal contaminants including Pb is adversely affected by metal adsorption to soil solids and/or precipitation as insoluble compounds. Addition of synthetic chelates has been shown to stimulate the release of metals into soil solution and enhance the potential for uptake into roots. A variety of synthetic chelates have this potential to induce Pb desorption from the soil matrix. Their effectiveness, in decreasing order is EDTA > HEDTA >DTPA >EGTA >EDDHA. This is known as Assisted Phytoextraction.
  5. Rhizofiltration is similar to phytoextraction, but the plants are used to clean up contaminated groundwater rather than soil.
  6. Contaminated water is either collected from a waste site and brought to the plants or the plants are planted in the contaminated area, where the roots then take up the water and the contaminants dissolved in it. As the roots become saturated with contaminants, they are harvested.
  7. Phytostabilisation is the use of certain plant species to immobilise contaminants in the soil and groundwater through absorption and accumulation by roots, adsorption onto roots, or precipitation within the root zone of plants (rhizosphere). This process reduces the mobility of the contaminant and prevents migration to the groundwater or air, and also reduces bioavailability for entry into the food chain . This technique can be used to re-establish a vegetative cover at sites where natural vegetation is lacking due to high metal concentrations in surface soils or physical disturbances to surficial materials. Metal-tolerant species can be used to restore vegetation to the sites, thereby decreasing the potential migration of contamination through wind erosion and transport of exposed surface soils and leaching of soil contamination to groundwater.
  8. Genetic engineering is a powerful method for enhancing natural phytoremediation capabilities, or for introducing new capabilities into plants Possibly, the most spectacular application of biotechnology for environmental restoration has been the bioengineering of plants capable of volatilizing mercury from soil contaminated with methyl-mercury. Methyl-mercury, a strong neurotoxic agents, is biosynthesized in Hg contaminated soils. To detoxify this toxin, transgenic plants (Arabidopsis and tobacco) were engineered to express bacterial genes merB and merA. In these modified plants, merB catalyzes the protonolysis of the carbon-mercury bond with the generation of Hg2+, a less mobile mercury species. Subsequently, MerA converts Hg(II) to Hg (0) a less toxic, volatile element which is released into the atmosphere .