PHYTOREMEDIATION
CONCEPTS AND
TECHNIQUES
Name: A. Ranga Rani
ID.NO:TAM-13-24
Phytoremediation
It is applied at sites with contamination of
 Organic Chemicals –TCE, TNT,, PCBs etc
 Nutrient – Ammonia, Phosphate, Nitrate
 Metal pollutants – Lead, Cadmium, Zinc,
Arsenic, Chromium
Phytoremediation is a process that uses plants to
remove, transfer, stabilize, and destroy
contaminants in soil and sediment.
Pteris vittata
Brassica juncea
Thlaspi caerulescens
Alyssum serpyllifolium
Hyperaccumulator plants
Poplar sps
PHYTOSTIMULATION
Phytoextraction
It is the uptake of contaminants,particularly toxic metals and radio
nuclei by plant roots and the translocate to the above ground parts.
Specially selected plants, known as hyperaccumulators, can extract and
accumulate exceptionally high levels of contaminants from soil.
Phytoextraction is achieved by two methods:
1. Continuous phytoextraction
2. Induced phytoextraction

1. Continuous phytoextraction
It is based on the phytological processes that allow plants
to accumulate metals over the complete growth cycle. It is
based on the genetic and physiological capacity of hyper
accumulators to accumulate,translocate and resist high
amount of metals
• The first hyper accumulators characterized were number of
Brasicaceae. Eg: Thallium- Iberis intermedia and
Biscutella laevigata of brassicaceae family,
• Zn in roots and shoots of Thalaspi arvense
• Brassica juncea in Pb, Zn and Cd
Chinese brake fern, P. vittata
to hyperaccumulate arsenic
Remediation of saline soils
Salicornia (pickleweed) accumulates salt in vacuole.
A form of table salt can then be extracted from plant.
Induced phytoextraction
• It is also known as chelate assisted phytoextraction.
Synthetic metal chelates such as EDTA addition to soil
increase the heavy metal accumulation by plants.
• Metal accumulation efficiency of plants is directly related
to the affinity of chelates for a particular metal.
• Eg. EDTA for Pb,Cd and citrate for U is normally
recommended for induced phytoextraction.
• Chelates applied at 5 and 10 mmol/kg to soil
Phytostabilization
Phytostabilization, also referred to as in place inactivation, is
primarily used for the remediation of soil, sediment, and
sludges
It is the use of plant roots to limit contaminant mobility and
bioavailability in the soil.
It is useful for the treatment of Pb as well as As, Cd, Cr, Cu
and Zn.
It is very effective when rapid immobilization is needed to
preserve ground and surface waters
Phytostabilization of mercury by willow roots
Yaodong Wang, 2004
Rhizofiltration
Rhizofiltration is primarily used to remediate extracted
groundwater, surface water, and wastewater with low contaminant
concentrations (Ensley, 2000).
 It is defined as the filtering of water through a mass of roots to
remove toxic substances or excess nutrients.
Rhizofiltration can be used for Pb, Cd, Cu, Ni, Zn, and Cr, which
are primarily retained within the roots
Indian mustard
water hyacinth
Duckweed (Lemna)
Azolla
Sunflower
Removal of substances from the
soil or water with release into the
air, possibly after degradation.
Indian mustard and canola
(Brassicanapus) may be effective
for phytovolatilization of
Selenium.

 Enhancement of microbial activity for the degradation
of contaminants, typically around plant roots
 PGPR bacteria produce heavy metal chelating agents
such as siderophores which results in sequestration of
metals and thus facilitates better crop growth in
polluted environment
Phytostimulation
PHYTOTRANSFORMATION
 Uptake of substances from the environment, and
degradation occurring in the plant
Trichloroethylene (TCE), a prevalent groundwater
contaminant, transformed to less toxic
metabolites by using hybrid poplar tree
Phytoremediation of pesticides by a notorious
pest plant
. This plant might be utilized as an efficient, economical and ecological alternative to accelerate the
removal and degradation of agro-industrial wastewater polluted with ethion.

Cost effective when compared to other more conventional
methods.
 “Nature” method, more aesthetically pleasing.
 Minimal land disturbance.
 Reduces potential for transport of contaminants by wind,
reduces soil erosion
Multiple contaminants can be removed with the same plant.
Disadvantages
 Success is dependant on the tolerance of the plant to
the pollutant
 Long length of time is required for remediation
 Possibility of contaminated plants entering the food
chain.
 Depends on the climate.
 Possible spread of contaminant through falling leaves.
THANK YOU

Phytoremediation tam 2013-24

  • 1.
  • 2.
    Phytoremediation It is appliedat sites with contamination of  Organic Chemicals –TCE, TNT,, PCBs etc  Nutrient – Ammonia, Phosphate, Nitrate  Metal pollutants – Lead, Cadmium, Zinc, Arsenic, Chromium Phytoremediation is a process that uses plants to remove, transfer, stabilize, and destroy contaminants in soil and sediment.
  • 4.
    Pteris vittata Brassica juncea Thlaspicaerulescens Alyssum serpyllifolium Hyperaccumulator plants Poplar sps
  • 6.
  • 7.
    Phytoextraction It is theuptake of contaminants,particularly toxic metals and radio nuclei by plant roots and the translocate to the above ground parts. Specially selected plants, known as hyperaccumulators, can extract and accumulate exceptionally high levels of contaminants from soil. Phytoextraction is achieved by two methods: 1. Continuous phytoextraction 2. Induced phytoextraction
  • 8.
     1. Continuous phytoextraction Itis based on the phytological processes that allow plants to accumulate metals over the complete growth cycle. It is based on the genetic and physiological capacity of hyper accumulators to accumulate,translocate and resist high amount of metals
  • 9.
    • The firsthyper accumulators characterized were number of Brasicaceae. Eg: Thallium- Iberis intermedia and Biscutella laevigata of brassicaceae family, • Zn in roots and shoots of Thalaspi arvense • Brassica juncea in Pb, Zn and Cd Chinese brake fern, P. vittata to hyperaccumulate arsenic
  • 10.
    Remediation of salinesoils Salicornia (pickleweed) accumulates salt in vacuole. A form of table salt can then be extracted from plant.
  • 11.
    Induced phytoextraction • Itis also known as chelate assisted phytoextraction. Synthetic metal chelates such as EDTA addition to soil increase the heavy metal accumulation by plants. • Metal accumulation efficiency of plants is directly related to the affinity of chelates for a particular metal. • Eg. EDTA for Pb,Cd and citrate for U is normally recommended for induced phytoextraction. • Chelates applied at 5 and 10 mmol/kg to soil
  • 13.
    Phytostabilization Phytostabilization, also referredto as in place inactivation, is primarily used for the remediation of soil, sediment, and sludges It is the use of plant roots to limit contaminant mobility and bioavailability in the soil. It is useful for the treatment of Pb as well as As, Cd, Cr, Cu and Zn. It is very effective when rapid immobilization is needed to preserve ground and surface waters
  • 14.
    Phytostabilization of mercuryby willow roots Yaodong Wang, 2004
  • 15.
    Rhizofiltration Rhizofiltration is primarilyused to remediate extracted groundwater, surface water, and wastewater with low contaminant concentrations (Ensley, 2000).  It is defined as the filtering of water through a mass of roots to remove toxic substances or excess nutrients. Rhizofiltration can be used for Pb, Cd, Cu, Ni, Zn, and Cr, which are primarily retained within the roots
  • 17.
  • 18.
    Removal of substancesfrom the soil or water with release into the air, possibly after degradation. Indian mustard and canola (Brassicanapus) may be effective for phytovolatilization of Selenium.
  • 19.
      Enhancement ofmicrobial activity for the degradation of contaminants, typically around plant roots  PGPR bacteria produce heavy metal chelating agents such as siderophores which results in sequestration of metals and thus facilitates better crop growth in polluted environment Phytostimulation
  • 21.
    PHYTOTRANSFORMATION  Uptake ofsubstances from the environment, and degradation occurring in the plant Trichloroethylene (TCE), a prevalent groundwater contaminant, transformed to less toxic metabolites by using hybrid poplar tree
  • 23.
    Phytoremediation of pesticidesby a notorious pest plant . This plant might be utilized as an efficient, economical and ecological alternative to accelerate the removal and degradation of agro-industrial wastewater polluted with ethion.
  • 24.
     Cost effective whencompared to other more conventional methods.  “Nature” method, more aesthetically pleasing.  Minimal land disturbance.  Reduces potential for transport of contaminants by wind, reduces soil erosion Multiple contaminants can be removed with the same plant.
  • 25.
    Disadvantages  Success isdependant on the tolerance of the plant to the pollutant  Long length of time is required for remediation  Possibility of contaminated plants entering the food chain.  Depends on the climate.  Possible spread of contaminant through falling leaves.
  • 26.