This document summarizes a seminar presentation on pharmacosomes. Pharmacosomes are colloidal dispersions of drugs that are covalently bound to lipids. They can exist as vesicular, micellar, or hexagonal aggregates depending on the drug-lipid complex. The presentation discusses the advantages of pharmacosomes over other drug delivery systems like liposomes, as well as their importance, formulation, evaluation, applications, and limitations. The key components and preparation methods for pharmacosomes are also outlined.
Aquasomes are nanoparticulate carrier system but instead of being simple nanoparticles these are three layered self assembled structures, comprised of a solid phase nanocrystalline core coated with oligomeric film to which biochemically active molecules are adsorbed with or without modification.
Pharmacosomes are the colloidal dispersions of drugs covalently bound to lipids, and may exist as ultrafine vesicular, micellar, or hexagonal aggregates, depending on the chemical structure of drug-lipid complex.
Aquasomes are nanoparticulate carrier system but instead of being simple nanoparticles these are three layered self assembled structures, comprised of a solid phase nanocrystalline core coated with oligomeric film to which biochemically active molecules are adsorbed with or without modification.
Pharmacosomes are the colloidal dispersions of drugs covalently bound to lipids, and may exist as ultrafine vesicular, micellar, or hexagonal aggregates, depending on the chemical structure of drug-lipid complex.
Barrier of drugs permeation through ocular route by Sushil Kumar SinghSushil Singh
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Three layered self assembled structures, containing the particle core composed of nanocrystalline calcium phosphate or ceramic diamond, and is covered by a polyhydroxyl oligomeric film to which biochemically active molecules are adsorbed.
SOLID DISPERSION
Definition: The technology is the science of dispersing one or more active ingredients in an inert matrix in the solid stage.
Need of solid dispersion:
Increases Oral bioavailability of a drug
Increased dissolution rate.
Enhanced release of drugs from ointment.
Improved the solubility & stability.
The concept of solid dispersion was originally proposed by Sekiguchi & obi.
Increasing the dissolution, absorption & therapeutic efficacy of drugs in dosage forms.
Increasing solubility in water.
Improving the oral absorption and bioavailability of BCS Class II drugs.
Liposomes are concentric bilayered vesicles in which an aqueous core is entirely enclosed by a membranous lipid bilayer mainly composed of natural or synthetic phospholipids.
Liposomes are spherical microscopic vesicles consisting phospholipids bilayers which enclose aqueous compartments.
The size of a liposome ranges from some 20 nm up to several micrometers.
Liposomes were first produced in England in 1961 by Alec D. Bangham, who was studying phospholipids and blood clotting.
Small unilamellar vesicles (SUV), 25 to 100 nm in size that consist of a single bilayer
Large unilamellar vesicle (LUV), 100 to 500 nm in size that consist of a single bilayer
Multilamellar vesicle (MLV), 200 nm to several microns, that consist of two or more concentric bilayer
NIOSOMES , GENERAL CHARACTERISTICS OF NIOSOME , TYPES OF NIOSOMES , OTHERS TYPES OF NIOSOMES , NIOSOMES VS LIPOSOMES , COMPONENTS OF NIOSOMES , Non-ionic surfactant , Cholesterol , Charge inducing molecule , METHOD OF PREPARATION , preparation of small unilamellar vesicles , Sonication , Micro fluidization , preparation of large unilamellar vesicles , Reverse Phase Evaporation , Ether Injection , preparation of Multilamellar vesicles , Hand shaking method , Trans membrane pH gradient drug uptake process (remote loading) , Miscellaneous method :Multiple membrane extrusion method , The “Bubble” Method , Formation of Niosomes From Proniosomes , SEPARATION OF UNENTRAPPED DRUGS , Gel Filtration , Dialysis , Centrifugation , FACTORS AFFECTING THE PHYSICOCHEMICAL PROPERTIES OF NIOSOMES , Membrane Additives , Temperature of Hydration , PROPERTIES OF DRUGS , AMOUNT AND TYPE OF SURFACTANT
Structure of Surfactants , Resistance to Osmotic Stress , Characterization of niosomes ,Therapeutic applications of Niosomes , For Controlled Release of Drugs , To Improve the Stability and Physical Properties of the Drugs , For Targeting and Retention of Drug in Blood Circulation , Proniosomes , Aspasomes , Vesicles in Water and Oil System (v/w/o) ,Bola - niosomes , Discomes , Deformable niosomes or elastic niosomes , According to the nature of lamellarity ,Small Unilamellar vesicles (SUV) 25 – 500 nm in size.,Large Unilamellar vesicles (LUV) 0.1 – 1μm in size , Multilamellar vesicles (MLV) 1-5 μm in size , According to the size:Small Niosomes (100 nm – 200 nm) , Large Niosomes (800 nm – 900 nm),Big Niosomes (2 μm – 4 μm)
An overview of Microspheres including Advantages, Types, Method of preparation, Materials used in preparations, Characterization or Evaluation and Applications.
Barrier of drugs permeation through ocular route by Sushil Kumar SinghSushil Singh
Barriers of Drugs Permeation Through Ocular Route. this topic explain about ocular route and barriers system. and classification of different injection routes takes the ocular drugs.
Three layered self assembled structures, containing the particle core composed of nanocrystalline calcium phosphate or ceramic diamond, and is covered by a polyhydroxyl oligomeric film to which biochemically active molecules are adsorbed.
SOLID DISPERSION
Definition: The technology is the science of dispersing one or more active ingredients in an inert matrix in the solid stage.
Need of solid dispersion:
Increases Oral bioavailability of a drug
Increased dissolution rate.
Enhanced release of drugs from ointment.
Improved the solubility & stability.
The concept of solid dispersion was originally proposed by Sekiguchi & obi.
Increasing the dissolution, absorption & therapeutic efficacy of drugs in dosage forms.
Increasing solubility in water.
Improving the oral absorption and bioavailability of BCS Class II drugs.
Liposomes are concentric bilayered vesicles in which an aqueous core is entirely enclosed by a membranous lipid bilayer mainly composed of natural or synthetic phospholipids.
Liposomes are spherical microscopic vesicles consisting phospholipids bilayers which enclose aqueous compartments.
The size of a liposome ranges from some 20 nm up to several micrometers.
Liposomes were first produced in England in 1961 by Alec D. Bangham, who was studying phospholipids and blood clotting.
Small unilamellar vesicles (SUV), 25 to 100 nm in size that consist of a single bilayer
Large unilamellar vesicle (LUV), 100 to 500 nm in size that consist of a single bilayer
Multilamellar vesicle (MLV), 200 nm to several microns, that consist of two or more concentric bilayer
NIOSOMES , GENERAL CHARACTERISTICS OF NIOSOME , TYPES OF NIOSOMES , OTHERS TYPES OF NIOSOMES , NIOSOMES VS LIPOSOMES , COMPONENTS OF NIOSOMES , Non-ionic surfactant , Cholesterol , Charge inducing molecule , METHOD OF PREPARATION , preparation of small unilamellar vesicles , Sonication , Micro fluidization , preparation of large unilamellar vesicles , Reverse Phase Evaporation , Ether Injection , preparation of Multilamellar vesicles , Hand shaking method , Trans membrane pH gradient drug uptake process (remote loading) , Miscellaneous method :Multiple membrane extrusion method , The “Bubble” Method , Formation of Niosomes From Proniosomes , SEPARATION OF UNENTRAPPED DRUGS , Gel Filtration , Dialysis , Centrifugation , FACTORS AFFECTING THE PHYSICOCHEMICAL PROPERTIES OF NIOSOMES , Membrane Additives , Temperature of Hydration , PROPERTIES OF DRUGS , AMOUNT AND TYPE OF SURFACTANT
Structure of Surfactants , Resistance to Osmotic Stress , Characterization of niosomes ,Therapeutic applications of Niosomes , For Controlled Release of Drugs , To Improve the Stability and Physical Properties of the Drugs , For Targeting and Retention of Drug in Blood Circulation , Proniosomes , Aspasomes , Vesicles in Water and Oil System (v/w/o) ,Bola - niosomes , Discomes , Deformable niosomes or elastic niosomes , According to the nature of lamellarity ,Small Unilamellar vesicles (SUV) 25 – 500 nm in size.,Large Unilamellar vesicles (LUV) 0.1 – 1μm in size , Multilamellar vesicles (MLV) 1-5 μm in size , According to the size:Small Niosomes (100 nm – 200 nm) , Large Niosomes (800 nm – 900 nm),Big Niosomes (2 μm – 4 μm)
An overview of Microspheres including Advantages, Types, Method of preparation, Materials used in preparations, Characterization or Evaluation and Applications.
PHARMACOSOME, METHODS OF PREPARATION OF PHARMACOSOME, APPLICATIONS, ADVANTAGES, DISADAVNTAGES OF PHARMACOSOME, FORMULATION OF PHARMACOSOME, COMPONENTS OF PHARMACOSOME, ACTION OF PHARMACOSOME
Vesicular systems have been realized as extremely useful carrier systems in various scientific domains. Over the years, vesicular systems have been investigated as a major drug delivery system, due to their flexibility to be tailored for varied desirable purposes. In spite of certain drawbacks, the vesicular delivery systems still play an important role in the selective targeting, and the controlled delivery of various drugs. Researchers all over the world continue to put in their efforts in improving the vesicular system by making them steady in nature, in order to prevent leaching of contents, oxidation, and their uptake by natural defense mechanisms.
Introduction,Drug- Excipient Compatibility Experimental Design ,Excipient role in drug destabilization,DRUG EXCIPIENT COMPATIBILTY IN PARENTERAL PRODUCTS.This topic are described.
Formulation and evaluation of Muco adhesive Buccal Tablets of Ramprildoddaapurupa
The buccal mucosa lines the inner cheek and Buccal formulations are placed in the mouth between upper gingiva(gums) and cheek to treat local and systemic conditions.
Drugs which undergoes Extensive first pass metabolism and drug degradation in acidic media, GI tract can be administered through buccal route.
The oral cavity has been used as a site for local and systemic drug delivery.
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Polymers are becoming increasingly important in the field of drug delivery. The pharmaceutical applications of polymers range from their use as binders in tablets to viscosity and flow controlling agents in liquids, suspensions and emulsions. Polymers can be used as film coatings to disguise the unpleasant taste of a drug, to enhance drug stability and to modify drug release characteristics.
As a consequence, increasing attention has been focused on methods of giving drugs continually for a prolonged time periods and in a controlled fashion.
This technology now spans many fields and includes pharmaceutical, food and agricultural applications, pesticides, cosmetics, and household products.
ATUL CHAUDHARY
STUDENT (M.PHARM, PHARMACEUTICS)
ISF, COLLEGE OF PHARMACY, GHALKALAN MOGA, PUNJAB
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Pharmacosomes
1. A Seminar on Pharmacosomes
By:
Shakeel Shaikh Shaikh Quader
M.Pharm (Pharmacuetics)
shakeelpharma4@gmail.com
Under the Guidance of
Prof. Siraj Shaikh
HOD of Pharmacuetics
Ali Allana COP Akkalkuwa
14/12/2017 1Shaikh Shakeel (AACOP Akkalkuwa)
3. Introduction:
To achieve targeted and controlled drug delivery various
approaches are used.
In which carrier mediated targeted DDS is best.
Different types of pharmaceutical carriers are polymeric,
articulate, micromolecules and cellular carriers.
Particulate types of carriers are also k/a Colloidal carrier
system which include lipids vesicles like liposomes,
niosomes, pharmacosomes and transferosomes.
14/12/2017 3Shaikh Shakeel (AACOP Akkalkuwa)
4. Most of the drugs, particularly chemotherapeutic agents,
have shown to have narrow therapeutic window, and their
clinical use is limited. Thus, their therapeutic effectiveness may
be increased by incorporating them in an advantageous manner.
In the past few decades, considerable attention had been
focused on the development of novel drug delivery system
(NDDS).
Lipid based drug delivery systems have been examined in
various studies and exhibited their potential in controlled and
targeted drug delivery. Pharmacosomes, a novel vesicular
drug delivery system, offering a unique advantage over
liposomes and niosomes, and serve as potential alternative to
these conventional vesicles.
14/12/2017 4Shaikh Shakeel (AACOP Akkalkuwa)
5. Pharmacosomes are the colloidal dispersions of drugs covalently
bound to lipids, and may exist as ultrafine vesicular, micellar, or
hexagonal aggregates, depending on the chemical structure of drug-
lipid complex.
As the system is formed by binding the drug (pharmakon) to
carrier (soma), they are termed as pharmacosomes.
The development of pharmacosomes depend upon the bulk and
surface interaction of the lipids with the particular drug.
Any drug having the active hydrogen atom like –COOH, -OH,
-NH2 etc are esterified to lipid with or without help of spacer chain
which strongly result in the formation of amphiphilic compound, that
can help in the cell wall transfer in the organism.
14/12/2017 5Shaikh Shakeel (AACOP Akkalkuwa)
7. Pharmacosomes impart better biopharmaceutical
properties to the drug, resulting in improved
bioavailability.
Pharmacosomes have been prepared for various non-
steroidal anti-inflammatory drugs, proteins,
cardiovascular and antineoplastic drugs.
Developing the pharmacosomes of the drugs has been
found to improve the absorption and minimize the
gastrointestinal toxicity.
14/12/2017 7Shaikh Shakeel (AACOP Akkalkuwa)
8. Advantage of Pharmacosomes
1. No leaching of drug takes place because the drug is
covalently bound to the carrier.
2. Drugs can be delivered directly to the site of infection.
3. Drug release from pharmacosomes is generally
governed by hydrolysis (including enzymatic).
4. Their degradation velocity into active drug molecule,
after absorption depends on their size and functional
groups of the drug molecule, the chain length of the
lipids, and spacer.
14/12/2017 8Shaikh Shakeel (AACOP Akkalkuwa)
9. 5. Reduced cost of therapy.
6. Suitable for both hydrophilic and lipophilic drugs. The
aqueous solution of these amphiphiles exhibits
concentration dependant aggregation.
7. High and predetermined entrapment efficiency of drug
and carrier are covalently linked together.
8. Volume of inclusion doesn’t influence on entrapment
efficiency.
9. No need of removing the free un-entrapped drug from
the formulation which is required in case of liposomes.
10. Improves bioavailability especially in case of poorly
soluble drugs.
11.Reduction in adverse effects and toxicity.
14/12/2017 9Shaikh Shakeel (AACOP Akkalkuwa)
10. 1. Pharamcosomes have some importance in escaping the tedious
steps of removing the free unentrapped drug from the
formulation.
2. Pharmacosomes provide an efficient method for delivery of drug
directly to the site of infection, leading to reduction of drug
toxicity with no adverse effects and also reduces the cost of
therapy by improved bioavailability of medication, especially in
case of poorly soluble drugs.
3. Pharmacosomes are suitable for incorporating both hydrophilic
and lipophilic drugs.
4. Entrapment efficiency is not only high but predetermined,
because drug itself in conjugation with lipids forms vesicles.
5. There is no need of following the tedious, time-consuming step
for removing the free, unentrapped drug from the formulation.
Important
14/12/2017 10Shaikh Shakeel (AACOP Akkalkuwa)
11. 6. Since the drug is covalently linked, loss due to leakage of drug,
does not take place.
7. No problem of drug incorporation
8. Encaptured volume and drug-bilayer interactions do not
influence entrapment efficiency, in case of pharmacosomes.
9. In pharmacosomes, membrane fluidity depends upon the phase
transition temperature of the drug lipid complex, but it does not
affect release rate since the drug is covalently bound.
10. The drug is released from pharmacosome by hydrolysis
(including enzymatic).
11. The physicochemical stability of the pharmacosome depends
upon the physicochemical properties of the drug-lipid complex.
14/12/2017 11Shaikh Shakeel (AACOP Akkalkuwa)
12. Limitations
1. Synthesis of a compound depends upon its amphiphilic
nature.
2. It requires surface and bulk interaction of lipids with
drugs.
3. It requires covalent bonding to protect the leakage of
drugs.
4. Pharmacosomes, on storage, undergo fusion and
aggregation, as well as chemical hydrolysis.
14/12/2017 12Shaikh Shakeel (AACOP Akkalkuwa)
13. Components used for the formulation of
pharmacosomes
There are three essential components for pharmacosomes
preparation.
Drugs
Drugs containing active hydrogen atom (-COOH, OH, NH2)
can be esterified to the lipid, with or without spacer chain and
they form amphiphilic complex which in turn facilitate
membrane, tissue, cell wall transfer in the organisms.
Solvents
For the preparation of pharmacosomes, the solvents should
have high purity and volatile in nature. A solvent with
intermediate polarity is selected for pharmacosomes
preparation.
14/12/2017 13Shaikh Shakeel (AACOP Akkalkuwa)
14. Lipids
Phospholipids are the major structure component of
biological membranes, where two types of phospholipids
such as phosphoglycerides and spingolipids are generally
used. The most common phospholipid is phosphotidyl
choline moiety. Phosphotidyl choline is an amphiphilic
molecule in which a glycerol bridges links a pair of
hydrophobic acyl hydrocarbon chains, with a hydrophilic
polar head group phosphocholine.
14/12/2017 14Shaikh Shakeel (AACOP Akkalkuwa)
15. Preparation:
Two methods have been used to prepare vesicles:
1.The hand-shaking method
2. The ether-injection method
14/12/2017 15Shaikh Shakeel (AACOP Akkalkuwa)
16. The hand-shaking method
In the hand-shaking method, the dried film of the drug–
lipid complex (with or without egg lecithin) is deposited
in a round-bottom flask and upon hydration with aqueous
medium, readily gives a vesicular suspension.
Surfactant / cholestrol mixture dissolve in the
diethylether in a round bottom flask and ether was
removed at room temperature under reduce pressure in a
rotatory evaporator.
The dried surfactant film was hydrated with an aqueous
phase at 50-60 C with gentle agitation.
14/12/2017 16Shaikh Shakeel (AACOP Akkalkuwa)
17. This method produce multilamellar vesicle with a large
diameter.
The lipophilic surfactant like span 40, span 60, span 80,
cholesterol and diacetyl phasphate can also be used in
hand shaking method.
14/12/2017 17Shaikh Shakeel (AACOP Akkalkuwa)
18. Fig. Hand Shaking method14/12/2017 18Shaikh Shakeel (AACOP Akkalkuwa)
19. Ether-injection method
In the ether-injection method, an organic solution of the
drug–lipid complex is injected slowly into the hot aqueous
medium, wherein the vesicles are readily formed.
14/12/2017 19Shaikh Shakeel (AACOP Akkalkuwa)
21. Drug salt was converted into the acid form to provide an active
hydrogen site
for complexation.
Drug acid was prepared by acidification of an aqueous solution
of drug salt, extraction into chloroform, and subsequent recrystallization.
Drug -PC complex was prepared by associating drug acid with an
equimolar
concentration of PC.
The equimolar concentration of PC and drug acid were placed in a 100-
mL round bottom flask and dissolved in dichloromethane.
The solvent was evaporated under vacuum at 40°C in a rotary vacuum
evaporator.
The pharmacosomes were collected as the dried residue and placed in a
vacuum desiccator overnight and then subjected to characterization.
Formulation of pharmacosomes:
14/12/2017 21Shaikh Shakeel (AACOP Akkalkuwa)
22. Pharmacosomes are evaluated for the following parameters.
Solubility:
To determine the change in solubility due to complexation,
solubility of drug acid and drug-PC complex was determined in pH
6.8 phosphate buffer and n-octanol by the shake-flask method.
Drug acid (50 mg) (and 50 mg equivalent in case of complex)
was placed in a 100-mL conical flask. Phosphate buffer pH 6.8 (50
mL) was added and then stirred for 15 minutes.
The suspension was then transferred to a 250 mL separating
funnel with 50 mL n-octanol and was shaken well for 30 minutes.
Then the separating funnel was kept still for about 30 minutes.
Concentration of the drug was determined from the aqueous layer
spectrophotometrically at 276 nm.
Evaluation of pharmacosomes:
14/12/2017 22Shaikh Shakeel (AACOP Akkalkuwa)
23. Drug content:
To determine the drug content in pharmacosomes of drug (e.g.:
diclofenac-PC complex), a complex equivalent to 50 mg diclofenac
was weighed and added into a volumetric flask with 100 mL of pH
6.8 phosphate buffer.
Then the volumetric flask was stirred continuously for 24 h on a
magnetic stirrer. At the end of 24 h, suitable dilutions were made
and measured for the drug content at 276 nm UV
spectrophotometrically.
Scanning electron microscopy (SEM):
To detect the surface morphology of the pharmacosomes, SEM of
the complex was recorded on a scanning electron microscope.
14/12/2017 23Shaikh Shakeel (AACOP Akkalkuwa)
24. Differential scanning calorimetry (DSC):
Thermograms of drug acid, phosphatidylcholine (80 %) and the
drug -PC complex were recorded using a 2910 Modulated
Differential Scanning Calorimeter V4.4E (TA Instruments, USA).
The thermal behavior was studied by heating 2.0 ± 0.2 mg of
each individual sample in a covered sample pan under nitrogen gas
flow. The investigations were carried out over the temperature
range 25–250 °C at a heating rate of 10 °C min–1.
14/12/2017 24Shaikh Shakeel (AACOP Akkalkuwa)
25. X-ray powder diffraction (XRPD):
The crystalline state of drug in the different samples was
evaluated using X-ray powder diffraction. Diffraction
patterns were obtained on a Bruker Axs- D8 Discover
Powder X-ray diffractometer, Germany.
The X-ray generator was operated at 40 kV tube
voltages and 40 mA tube current, using lines of copper as
the radiation source. The scanning angle ranged from 1
to 60° of 2q in the step scan mode (step width 0.4° min–
1).
Drug acid, phosphatidylcholine 80 % (Lipoid S-80) and
the prepared complex were analyzed.
14/12/2017 25Shaikh Shakeel (AACOP Akkalkuwa)
26. Dissolution study:
In vitro dissolution studies of drug –PC complex as well as plain
diclofenac acid were performed in triplicate in a USP (8) six station
dissolution test apparatus, type II (Veego Model No. 6 DR, India)
at 100 rpm and at 37 °C.
An accurately weighed amount of the complex equivalent to 100
mg of drug acid was put into 900 mL of pH 6.8 phosphate buffer.
Samples (3 mL each) of dissolution fluid were withdrawn at
different intervals and replaced with an equal volume of fresh
medium to maintain sink conditions.
Withdrawn samples were filtered (through a 0.45-mm membrane
filter), diluted
suitably and then analyzed spectrophotometrically at 276 nm.
14/12/2017 26Shaikh Shakeel (AACOP Akkalkuwa)
27. Entrapment efficiancy
E.F =Amount entraped / total amount x 100
VESICLES DIAMETER:
Using freeze fracture electron microscope and light
microscope
14/12/2017 27Shaikh Shakeel (AACOP Akkalkuwa)
28. Applications:
Targeted Drug delivery:
Delivery of Peptide drug:
E.g: 9-desglycinamide, 8-arginine vasopressin.
Carrier for FB
Development of Novel ophthalmic DDS
Pharmacosomes elicit greater shelf stability.
The approach has successfully improved the therapeutic
performance of various drugs i.e. pindolol maleate,
bupranolol hydrochloride, taxol, acyclovir, etc.
The phase transition temperature of pharmacosomes in the
vesicular and Micellar state could have significant
influence on their interaction with membranes.14/12/2017 28Shaikh Shakeel (AACOP Akkalkuwa)
29. Pharmacosomes can interact with bimembranes enabling a
better transfer of active ingredient. This interaction leads to
change in phase transition temperature of bimembranes
thereby improving the membrane fluidity leading to enhance
permeations.
Pharmacosomes have greater degree of selectivity for action
on specific target cells.
14/12/2017 29Shaikh Shakeel (AACOP Akkalkuwa)
30. Drug Therapeutic Application of
Drugs after incorporation
with Pharmacosomes.
Pindolol diglyceride Three to five fold increase in
plasma concentration Lower
renal clearance [
Amoxicillin Improved cytoprotection and
treatment of H.pylori infections
in male rats.
Taxol Improved biological activity
Cytarbin Improved biological activity
Dermatan sulfate Improved biological activity
Bupranolol hydrochloride Enhanced effect on intraocular
pressure.
Enhance lymph transport
14/12/2017 30Shaikh Shakeel (AACOP Akkalkuwa)
31. Conclusion:
Vesicular systems have been realized as extremely useful carrier
systems in various scientific domains. Over the years, vesicular
systems have been investigated as a major drug delivery system,
due to their flexibility to be tailored for varied desirable purposes.
In spite of certain drawbacks, the vesicular delivery systems still
play an important role in the selective targeting, and the controlled
delivery of various drugs. Researchers all over the world continue
to put in their efforts in improving the vesicular system by making
them steady in nature, in order to prevent leaching of contents,
oxidation, and their uptake by natural defense mechanisms.
14/12/2017 31Shaikh Shakeel (AACOP Akkalkuwa)
32. References:
Dr. Dheeraj T. Baviskar and Dr. Dinesh K. Jain, “Novel drug delivery
System” Second Edition, March 2015, Published by Nirali Prakashan, PGE
No: 14.37-14.40
Vyas .S.P “Theory and practical in novel drug delivery system” First edition
2009, published by CBS publisher and distributors, page no: 148-160
Y. Jin et al., "Self-Assembled Drug Delivery Systems-Properties and In Vitro
–In Vivo Behaviour of Acyclovir Self-Assembled Nanoparticles (san)," Int. J.
Pharm. 309 (1–2), 199–207 (2006).
P. Goyal et al., "Liposomal Drug Delivery Systems: Clinical Applications,"
Acta Pharm. 55, 1–25 (2005).
H.A. Lieberman, M.M. Rieger, and G.S. Banker, Pharmaceutical Dosage
Forms: Disperse Systems (Informa Healthcare, London, England, 1998), p.
163.
. S.S. Biju et al., "Vesicular Systems: An Overview," Ind. Jour. Pharm. Sci. 68
(2), 141–153 (2006).
14/12/2017 32Shaikh Shakeel (AACOP Akkalkuwa)