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Phytochemistry
S.Y.B.Sc. Botany Paper III
Unit 1
Part 2
Phytochemistry
Tannins
Phytochemistry
Tannins
Phytochemistry
Tannins
Phytochemistry
Tannins
Phytochemistry
Tannins
Definition
• The substances present in plant extracts which
are able to combine with protein of animal
hides, prevent their putrification and convert
them into leather
• The substances which tan leather are referred
to as Tannins
Phytochemistry
Tannins
• Recently, tannins have been defined as the
derivatives of polyhydroxy benzoic acid
capable of combining with proteins
Phytochemistry
Tannins
Structure
Benzoic Acid
Protocatechuic Acid Gallic Acid Glucogallin
Ellagic Acid Hexahydroxydiphenic Acid
Phytochemistry
Tannins
Distribution – (Plant Source)
– Large group of complex substances
– Usually found in largest quantity in dead and dying
cells
– Localized in specific plant parts such as Leaves,
fruits, bark and stem
– Probably serve for the protection of the plant
during certain stages of growth
– Destroyed or deposited as end product
Phytochemistry
Tannins
Plant sources – (In general)
– Bark – Cinnamon, Cinchona, Acacia,
Pomegranate
– Roots & Rhizome – Fern
– Leaves – Tea, especially Green tea,
Eucalyptus
– Seeds – Cocoa, Kola and Areca
– Flower&floral parts – Clove, Red rose petals
– Fruits – Myrobalans (Terminalia chebula,
T. belerica) Pomegranate rind
Phytochemistry
Tannins
Extraction of Tannins
Depending upon
various sources
Different
Methods are
employed
Solvents Used
Ether
Methanol
Acetone
Ethyl acetate
Water
Clear extract
dried under
vaccum
Phytochemistry
Tannins
Classification of Tannins
– Hydrolysable Tannins or Pyrogallotannins
– Condensed Tannins or Phlobatannins
– Pseudotannins
Phytochemistry
Tannins
Hydrolysable Tannins or Pyrogallotannins
– They are readily hydrolysed by acids or enzymes such as
Tannase
– They are formed from several molecules of phenolic
acids such as Gallic acid, Ellagic acid etc.
Hydrolysable tannins + HCl boil Gallic acid or Ellagic Acid
H. Tannins + FeCl3 Blue Colour
H. Tannins + Br2 water No ppt.
Examples : Clove, Eucalyptus leaves, Harda, Behda
Phytochemistry
Tannins
Condensed Tannins or Pyrocatechol or Phlobatannins
• These include all other true tannins
• Their molecules are more resistant to breakage than hydrolysable
tannins
• They yield catechol on dry distillation therefore they are called as
catechol tannins
Condensed tannins + HCl boil Red insoluble Phlobophene
C. Tannins + FeCl3 Green Colour
C. Tannins + Br2 water Forms ppt.
Examples : Cinnamon, Cinchona, Acacia, Cocoa Seed, Areca Seed,
Kola nut & Green tea leaves
Phytochemistry
Tannins
Pseudotannins
– They are compounds of low molecular weight
than true tannins
– They do not tan leather
Pseudo Tannins + FeCl3 Purple to Violet Colour
Example : Catechin present in Catechu (Acacia catechu)
Chlorogenic Acid in Coffee ( mostly unroasted)
Nux vomica seeds
Phytochemistry
Tannins
Properties of Tannins
– Non crystallizable
– Form colloidal solution with water
– Soluble in dil. alkali, alcohol & acetone
– Solution give acid reaction
– Sharp astringent taste
– With ferric salts they form dark blue or greenish
black soluble compounds
– They precipitate proteins
Phytochemistry
Tannins
Uses of Tannins:
– Used as antiseptic in inflammation, piles, burns
etc.( Phenol group is responsible)
– It is also used in gastrointestinal diseases
(Diarrhoea)
– Carcinogenic on prolong use
Pharmacognosy part 2

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Pharmacognosy part 2