BioPerl is an active open source software project supported by the Open Bioinformatics Foundation.
BioPerl is a product of community effort to produce Perl code which is useful in biology.
BioPerl is a collection of Perl modules
It has played an integral role in the Human Genome Project
Secondary Structure Prediction of proteins Vijay Hemmadi
Secondary structure prediction has been around for almost a quarter of a century. The early methods suffered from a lack of data. Predictions were performed on single sequences rather than families of homologous sequences, and there were relatively few known 3D structures from which to derive parameters. Probably the most famous early methods are those of Chou & Fasman, Garnier, Osguthorbe & Robson (GOR) and Lim. Although the authors originally claimed quite high accuracies (70-80 %), under careful examination, the methods were shown to be only between 56 and 60% accurate (see Kabsch & Sander, 1984 given below). An early problem in secondary structure prediction had been the inclusion of structures used to derive parameters in the set of structures used to assess the accuracy of the method.
Some good references on the subject:
Secondary Structure Prediction of proteins Vijay Hemmadi
Secondary structure prediction has been around for almost a quarter of a century. The early methods suffered from a lack of data. Predictions were performed on single sequences rather than families of homologous sequences, and there were relatively few known 3D structures from which to derive parameters. Probably the most famous early methods are those of Chou & Fasman, Garnier, Osguthorbe & Robson (GOR) and Lim. Although the authors originally claimed quite high accuracies (70-80 %), under careful examination, the methods were shown to be only between 56 and 60% accurate (see Kabsch & Sander, 1984 given below). An early problem in secondary structure prediction had been the inclusion of structures used to derive parameters in the set of structures used to assess the accuracy of the method.
Some good references on the subject:
protein structure prediction methods. homology modelling, fold recognition, threading, ab initio methods. in short and easy form slides. after one time read you can easily understand methods for protein structure prediction.
After sequencing of the genome has been done, the first thing that comes to mind is "Where are the genes?". Genome annotation is the process of attaching information to the biological sequences. It is an active area of research and it would help scientists a lot to undergo with their wet lab projects once they know the coding parts of a genome.
As an interdisciplinary field of science, bioinformatics combines biology, computer science, information engineering, mathematics and statistics to analyze and interpret the biological data.
This presentation gives you a detailed information about the swiss prot database that comes under UniProtKB. It also covers TrEMBL: a computer annotated supplement to Swiss-Prot.
Ab Initio Protein Structure Prediction is a method to determine the tertiary structure of protein in the absence of experimentally solved structure of a similar/homologous protein. This method builds protein structure guided by energy function.
I had prepared this presentation for an internal project during my masters degree course.
Clustering and Visualisation using R programmingNixon Mendez
Clustering Analysis is a collection of patterns into clusters based on similarity.
Here we will discuss on the following :
Microarray Data of Yeast Cell Cycle
Clustering Analysis :-
Principal Component Analysis (PCA)
Multidimensional Scaling (MDS)
K-Means
Self-Organizing Maps (SOM)
Hierarchical Clustering
protein structure prediction methods. homology modelling, fold recognition, threading, ab initio methods. in short and easy form slides. after one time read you can easily understand methods for protein structure prediction.
After sequencing of the genome has been done, the first thing that comes to mind is "Where are the genes?". Genome annotation is the process of attaching information to the biological sequences. It is an active area of research and it would help scientists a lot to undergo with their wet lab projects once they know the coding parts of a genome.
As an interdisciplinary field of science, bioinformatics combines biology, computer science, information engineering, mathematics and statistics to analyze and interpret the biological data.
This presentation gives you a detailed information about the swiss prot database that comes under UniProtKB. It also covers TrEMBL: a computer annotated supplement to Swiss-Prot.
Ab Initio Protein Structure Prediction is a method to determine the tertiary structure of protein in the absence of experimentally solved structure of a similar/homologous protein. This method builds protein structure guided by energy function.
I had prepared this presentation for an internal project during my masters degree course.
Clustering and Visualisation using R programmingNixon Mendez
Clustering Analysis is a collection of patterns into clusters based on similarity.
Here we will discuss on the following :
Microarray Data of Yeast Cell Cycle
Clustering Analysis :-
Principal Component Analysis (PCA)
Multidimensional Scaling (MDS)
K-Means
Self-Organizing Maps (SOM)
Hierarchical Clustering
Lyme disease, the most common vector-borne illness in the United States, is a multisystem illness caused by infection with the spirochete Borrelia burgdorferi and the body's immune response to the infection. The disease is transmitted to humans via tick bites, from infected ticks of the genus Ixodes.
Structural Bioinformatics - Homology modeling & its ScopeNixon Mendez
Homology modeling also known as comparative modeling uses homologous sequences with known 3D structures for the modelling and prediction of the structure of a target sequence
Homology modeling is one of the most best performing prediction methods that gives “accurate” predicted models.
Errors and Limitaions of Next Generation SequencingNixon Mendez
High throughput sequencing technologies has made whole genome sequencing and resequencing available to many more researchers and projects.
Cost and time have been greatly reduced.
The error profiles and limitations of the new platforms differ significantly from those of previous sequencing technologies.
The selection of an appropriate sequencing platform for particular types of experiments is an important consideration.
NGS sequencing errors focuses mainly on the following points:
1.Low quality bases
2.PCR errors
3.High Error rate
NGS has inherent limitations they are as follows :
1.Sequence properties and algorithmic challenges
2.Contamination or new insertions
3.Repeat content
4.Segmental duplications
5.Missing and fragmented genes
6.Reference index
Addressing the shortage of medical doctors in zambiaNixon Mendez
An expert system is a computer system that emulates the decision making ability of a human expert.
Zambia faces a severe shortage of medical personnel, especially medical doctors due to migration and limited number of medical schools. Rural areas are mostly affected.
Other causes of the inadequate number of trained health personnel.
Early retirement by health.
Change in careers.
Some tend to join politics.
Some specialist health workers get infected with HIV- AIDS.
PowerMV is a software environment for statistical analysis, molecular viewing, descriptor generation, and similarity search.
In this presentation we will study about two modules nearest neighbor search an molecular descriptor generation.
Mascot is a software package from Matrix Science that interprets mass spectral data into protein identities.
In this presentation we will study about MASCOT and also on how to use it.
2D-PAGE is a method is used for the separation and identification of proteins in a complex mixture using two separate dimensions that are run perpendicular to one another.
2D-DIGE is an advanced version of classical two-dimensional gel electrophoresis (2D-PAGE).
The protein samples are labeled with fluorescent dyes and then separated by 2D-PAGE.
Cytoscape plugins - GeneMania and CentiScapeNixon Mendez
Cytoscape is an open source software platform. It provides features for data integration, analysis and visualization.
Additional features are available in the form of apps (plugins).
These apps can be used for network analyses, molecular scripting and connection with databases.These apps are available in Cytoscape App Store.
Collaboratively Creating the Knowledge Graph of LifeChris Mungall
Overview of collaborative projects in the life sciences building out the necessary ontologies, schemas, and knowledge graphs for describing biological knowledge
Conservation of Scientific Workflow Infrastructures by Using Semantics - 2012Idafen Santana Pérez
Slides from a weekly talk at OEG in December 2012, focusing on defining the main concepts for my thesis, including what we understand by reproducibility, replicability, conservation, and preservation.
What can you learn from molecular modeling?digitalbio
A presentation given by Dr. Sandra Porter at the Biotechnology Educators Conference, Virginia Bioinformatics Institute, July 2015
For more information, see http://DigitalWorldBiology.com
We have established an unassailable reputation for our Project Training and Professional Certification Programs on the sheer virtue of our intensive focus on making our students “Industry-Ready”.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
2. • BioPerl is an active open source software project supported by
the Open Bioinformatics Foundation.
• BioPerl is a product of community effort to produce Perl code which is
useful in biology.
• BioPerl is a collection of Perl modules
• It has played an integral role in the Human Genome Project
• Sequence objects
• Alignment objects
• Database searching objects
• They interact with each other
BioPerl
3. • Object Oriented
• Modules and Objects
• Flexible and Simple
• Deals with Biological Data
• Objects are created by Modules
• Objects are the container of data
Bioperl
5. • It is central Module
• Using Bio::Seq module we can create Bio::Seq object
Flavours of Seq objects
- Bio::PrimarySeq
- Bio::SeqFeature
- Bio::Seq
Bio::Seq
6. Example 1:
use Bio::Seq;
$seq_obj = Bio::Seq->new(-seq => "aaaatgggggggggggccccgtt",
-display_id => "#12345",
-desc => "example 1",
-alphabet => "dna" );
print $seq_obj->seq();
Creating a sequence and an Object
8. • Handler for SeqIO Formats
• Used for biological file handles
• Read or Write sequences
• Supported formats include fasta, genbank, embl, swiss
(SwissProt), Entrez Gene and tracefile formats such as abi (ABI)
and scf. quence objects
Bio::SeqIO
10. Example 3:
use Bio::Seq;
use Bio::SeqIO;
$seq_obj = Bio::Seq->new(-seq =>
"aaaatgggggggggggccccgtt",
-display_id => "#12345",
-desc => "example 1",
-alphabet => "dna" );
$seqio_obj = Bio::SeqIO->new(-file => '>sequence.fasta', -
format => 'fasta' );
$seqio_obj->write_seq($seq_obj);
Writing a sequence to a file
11. Example 4:
use Bio::SeqIO;
$seqio_obj = Bio::SeqIO->new(-file => "sequence.fasta", -format
=> "fasta" );
$seq_obj = $seqio_obj->next_seq;
print $seq_obj->seq,"n";
Retrieving a sequence from a file
12. Example 5:
use Bio::SeqIO;
$seqio_obj = Bio::SeqIO->new(-file => "sequence.fasta", -format
=> "fasta" );
while ($seq_obj = $seqio_obj->next_seq)
{
print $seq_obj->seq,"n";
}
Retrieving multiple sequence from a
file