This document outlines a study on the histopathological findings of gastrointestinal endoscopic biopsies. The study will involve collecting approximately 600 biopsy samples from patients undergoing endoscopy at MGMCRI in India from 2016-2018. Biopsy samples will be examined histopathologically and findings will be analyzed. The aim is to study the spectrum of gastrointestinal lesions identified and correlate histological findings with clinical and endoscopic details. Statistical analysis will be performed to compare results with other studies. The study involves collection of human biopsy samples and aims to improve diagnosis of gastrointestinal diseases.

1. “A CLINICOPATHOLOGICAL STUDY OF
GASTROINTESTINAL ENDOSCOPIC BIOPSIES”
CANDIDATE :
Dr. JANANI MATHILAGAN
1st YEAR POST GRADUATE
DEPARTMENT OF PATHOLOGY
MGMCRI
GUIDE:
Dr. DHANANJAY S. KOTASTHANE MD
PROFESSOR AND HEAD
DEPARTMENT OF PATHOLOGY,
MGMCRI
Co-GUIDE:
DR. TIROU AROUL MS
PROFESSOR
DEPARTMENT OF GENERAL
SURGERY, MGMCRI
Co-GUIDE:
DR.SAJEETH MANIKANDA PRABU DM
ASSISTANT PROFESSOR
DEPARTMENT OF MEDICAL
GASTROENTEROLOGY, MGMCRI

2. INTRODUCTION
• GI endoscopy in combination with biopsy plays an
important role in the early diagnosis of GI neoplasms.
• Endoscopic screening may detect gastric mucosal lesions
at an early stage so as to prevent progression of lesions
to invasive cancer.
• Biopsy and histological assessment provide a critical
adjunct to endoscopic assessment of the gastrointestinal
tract.
• In diseases such as cancer, coeliac disease and chronic
inflammatory bowel disease, pathological diagnosis
remains the gold standard.

3. REVIEW OF LITERATURE
• Shanmugasamy K,et al. conducted a study on Clinical correlation of upper
gastrointestinal endoscopic biopsies with histopathological findings and
found there were 24 neoplastic lesions (21%) and 91 non-neoplastic
lesions (79%) were observed out of 115 cases.
• Shefali.H.Karve et al. conducted a clinico-pathological study of colonic
biopsies in which out of 159 colonoscopic biopsies performed, 68 cases
were diagnosed as non-neoplastic lesions, out of which 32 cases of Non-
specific colitis, 11 cases of ulcerative colitis, 5 cases of juvenile polyps, 5
cases of hyperplastic polyps, 4 cases of SRUS, 3 cases of Granulomatous
inflammation, 3 cases of Retention polyp, 2 cases of Crohn’s disease and
one case each of Acute inflammation, Inflammatory polyp and
Endometriosis.
• Rangaswamy R et al conducted a Clinico-colonoscopic and
Histomorphological study on Spectrum of Colonic Diseases and found that
out of 107 biopsies, 76.64% biopsies were non-neoplastic and 23.36%
biopsies were neoplastic lesions.

4. AIMS & OBJECTIVES
1. To study the histopathological spectrum of endoscopic
gastrointestinal biopsies in MGMCRI, Pondicherry.
2. To correlate histopathological findings with clinical
history and endoscopic findings.

5. • STUDY SUBJECTS
This study involves HUMANS.
• TYPE OF STUDY
This is a descriptive study.
• PERIOD OF STUDY
From December 2016 to May 2018
• STUDY POPULATION
All patients reporting with gastrointestinal lesions and whose
tissues were removed via endoscope for diagnostic purposes.
• INCLUSION CRITERIA
Endoscopic biopsy for lesions present in upper GIT.
Colonoscopic biopsy for lesions present in lower GIT
• EXCLUSION CRITERIA
Not Applicable.
• SAMPLE SIZE
Approximately 600 cases.

6. METHODS
`
Brief clinical data regarding age, sex, clinical findings and endoscopic findings
are noted
Specimen received in 10% formalin will be fixed and subjected to gross
examination.
Sections will be processed followed by paraffin embedding
3-5 micrometers thin multiple sections will be taken & stained with
hematoxylin and eosin.
Findings will be analyzed & compared with that of other authors.
Histopathology of gastrointestinal lesions will be studied.

7. STATISTICS
• Statistical methods such as Pie charts and Bar diagram will be
used for descriptive purpose.
• Chi-Square test will be used to determine significance
between parameters observed in this study with similar
studies of other authors.
• P < 0.05 will be accepted as significant.

8. ETHICAL ISSUES
The Study Involves:
Collection of biopsy